{"id":43400,"date":"2020-10-21T14:30:00","date_gmt":"2020-10-21T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/ajoutez-les-chaines-ultra-courtes-a-votre-analyse-totale-de-pfas\/"},"modified":"2026-01-28T19:03:29","modified_gmt":"2026-01-28T19:03:29","slug":"ajoutez-les-chaines-ultra-courtes-a-votre-analyse-totale-de-pfas","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/notes-dapplication\/evan3073-fr\/ajoutez-les-chaines-ultra-courtes-a-votre-analyse-totale-de-pfas","title":{"rendered":"Ajoutez les cha\u00eenes ultra-courtes \u00e0 votre analyse totale de PFAS"},"content":{"rendered":"\n

R\u00e9sum\u00e9<\/h2>\n\n\n\n

Une m\u00e9thode LC-MS\/MS simple, par injection directe, a \u00e9t\u00e9 d\u00e9velopp\u00e9e et \u00e9valu\u00e9e pour l\u2019analyse simultan\u00e9e des PFAS (Substances Per- et PolyFluoroAlkyl\u00e9es) r\u00e9glement\u00e9s, alternatifs et \u00e0 cha\u00eenes ultra-courtes dans diff\u00e9rents types d\u2019eaux. Cette m\u00e9thode est recommand\u00e9e pour les laboratoires souhaitant n\u2019utiliser qu\u2019une seule proc\u00e9dure pour analyser ces trois cat\u00e9gories de PFAS dans les eaux potables et non-potables.<\/p>\n\n\n\n

Introduction<\/h2>\n\n\n\n

Les m\u00e9thodes LC-MS\/MS pour l\u2019analyse des PFAS (Substances Per- et PolyFluoroAlkyl\u00e9es) r\u00e9glement\u00e9s \u00e0 cha\u00eenes courtes (C4, C5) et longues (>C5), bas\u00e9es sur la chromatographie de phase inverse sont bien \u00e9tablies. Avec des modifications appropri\u00e9es, ces m\u00e9thodes peuvent souvent \u00eatre utilis\u00e9es pour l\u2019analyse LC-MS\/MS de PFAS alternatifs, tels que l\u2019HFPO-DA (GenX) ou l\u2019ADONA, qui sont des acides perfluoroalkyl-\u00e9ther-carboxyliques utilis\u00e9s comme substituts au PFOA. De la m\u00eame mani\u00e8re, le F-53B est une alternative au PFOS, produite en Chine et qui contient deux compos\u00e9s polyfluoroalkyl-\u00e9ther-sulfonate, le 9Cl-PF3ONS et le 11Cl-PF3OUdS, qui sont inclus dans la liste d\u2019analytes de la m\u00e9thode EPA 537.1 suite \u00e0 sa derni\u00e8re r\u00e9vision. Cependant, les m\u00e9thodes LC actuelles peuvent ne pas convenir pour l\u2019analyse des PFAS \u00e0 cha\u00eenes ultra-courtes, PFAS qui suscitent de plus en plus d\u2019int\u00e9r\u00eat, principalement en raison d\u2019une r\u00e9tention trop faible sur les colonnes usuelles de phase inverse.<\/p>\n\n\n\n

Bien que l\u2019utilisation des PFAS \u00e0 cha\u00eenes courtes (PFBA et PFBS) soit intentionnelle, de nombreuses \u00e9tudes ont montr\u00e9 l\u2019omnipr\u00e9sence des PFAS C2 et C3 dans des \u00e9chantillons environnementaux aqueux [1,2]. Ceux-ci comprennent l\u2019acide trifluoroac\u00e9tique (TFA), l\u2019acide perfluoropropano\u00efque (PFPrA), le perfluoro\u00e9thane-sulfonate (PFEtS) et le perfluoropropane-sulfonate (PFPrS). Il a \u00e9t\u00e9 d\u00e9montr\u00e9 que le PFPrA est le PFAS pr\u00e9dominant (jusqu\u2019\u00e0 45% du total des PFAS d\u00e9tectables) dans des \u00e9chantillons de pluie et de neige pr\u00e9lev\u00e9s aux \u00c9tats-Unis, en France et au Japon [3]. \u00c0 ce jour, les sources de ces contaminations par les PFAS \u00e0 cha\u00eenes ultra-courtes et leurs niveaux ne sont pas enti\u00e8rement d\u00e9finis, mais une \u00e9tude r\u00e9cente a d\u00e9tect\u00e9 du PFEtS et du PFPrS dans des mousses filmog\u00e8nes aqueuses (AFFF) et des eaux souterraines de 11 bases militaires aux \u00c9tats-Unis (souvent utilis\u00e9es pour des exercices de formation des pompiers) [4], indiquant que ces mousses anti-incendie peuvent \u00eatre une source de PFAS \u00e0 cha\u00eene ultra-courte.<\/p>\n\n\n\n

Actuellement, les m\u00e9thodes permettant l\u2019analyse combin\u00e9e des PFAS \u00e0 cha\u00eenes ultra-courtes avec les PFAS r\u00e9glement\u00e9s et alternatifs sont tr\u00e8s rares. Pour combler ce manque, nous avons d\u00e9velopp\u00e9 une m\u00e9thode pour la quantification simultan\u00e9e d\u2019une large gamme de longueurs de cha\u00eene et de structures, y compris les PFAS C3, C4, C8 et alternatifs, dans diff\u00e9rents types d\u2019eaux.<\/p>\n\n\n\n

Proc\u00e9dure<\/h2>\n\n\n\n

Pr\u00e9paration des \u00e9chantillons<\/h4>\n\n\n\n

Dans un flacon en polypropyl\u00e8ne, 250 \u00b5l d\u2019\u00e9chantillon d\u2019eau ont \u00e9t\u00e9 m\u00e9lang\u00e9s avec 250 \u00b5L d\u2019une solution eau\/m\u00e9thanol 40\/60 et 5 \u00b5L d\u2019une solution de standards internes (5 ng\/mL de 13<\/sup>C2<\/sub>-PFHxA, 13<\/sup>C2<\/sub>-PFOA, 13<\/sup>C4<\/sub>-PFOS dans le m\u00e9thanol). Le flacon a \u00e9t\u00e9 ferm\u00e9 \u00e0 l\u2019aide d\u2019un bouchon en poly\u00e9thyl\u00e8ne et son contenu ensuite inject\u00e9 pour analyse.<\/p>\n\n\n\n

\u00c9talons et \u00c9chantillons CQ<\/h4>\n\n\n\n

Les \u00e9talons ont \u00e9t\u00e9 pr\u00e9par\u00e9s en dopant une eau de qualit\u00e9 LC-MS (Optima) avec 10 PFAS sur une gamme allant de 5 \u00e0 400 ng\/L. Les solutions \u00e9talons ont ensuite \u00e9t\u00e9 trait\u00e9es comme d\u00e9crit dans la proc\u00e9dure de pr\u00e9paration des \u00e9chantillons<\/p>\n\n\n\n

\u00c9chantillons d\u2019eau dop\u00e9s<\/h4>\n\n\n\n

De l\u2019eau du robinet de chez Restek ainsi que trois autres types d\u2019eau diff\u00e9rents (eau de la rivi\u00e8re Chicago, eau souterraine et eau de sortie d\u2019usine de traitement publique [POTW]) fournis par l\u2019EPA des \u00c9tats-Unis ont \u00e9t\u00e9 inclus dans cette \u00e9tude. Chaque type d\u2019eau a \u00e9t\u00e9 dop\u00e9 \u00e0 10 (20 ppt pour le PFPrA) et 80 ppt, en double par lot avec un total de 3 lots \u00e9tant pr\u00e9par\u00e9s et analys\u00e9s \u00e0 des jours diff\u00e9rents. Les \u00e9chantillons dop\u00e9s et non-dop\u00e9s ont \u00e9t\u00e9 trait\u00e9s en suivant la proc\u00e9dure de pr\u00e9paration des \u00e9chantillons, pour analyse chromatographique et quantification.<\/p>\n\n\n\n

Conditions instrumentales<\/h4>\n\n\n\n

L’analyse simultan\u00e9e des PFAS \u00e0 cha\u00eenes ultra-courtes avec les PFAS r\u00e9glement\u00e9s et alternatifs a \u00e9t\u00e9 r\u00e9alis\u00e9e \u00e0 l\u2019aide d\u2019une colonne analytique Raptor C18 et d\u2019une cha\u00eene LC Shimadzu Nexera X2 coupl\u00e9e \u00e0 une MS\/MS SCIEX 4500. Une “colonne-retard” (r\u00e9f. 27854) a \u00e9t\u00e9 install\u00e9e entre le mixer et l\u2019injecteur afin d\u2019\u00e9viter la co\u00e9lution de tout PFAS provenant de l\u2019instrument avec des PFAS pr\u00e9sents dans l\u2019\u00e9chantillon. Les conditions instrumentales \u00e9taient les suivantes et les transitions MRM des analytes sont fournies dans le Tableau I.<\/p>\n\n\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Colonne analytique :<\/td>\nRaptor C18 2.7 \u00b5m, 3.0 mm x 100 mm (r\u00e9f. 9304A1E)<\/td>\n<\/tr>\n
“Colonne-retard” :<\/td>\n“colonne-retard” PFAS (r\u00e9f. 27854)<\/td>\n<\/tr>\n
Phase mobile A :<\/td>\nAc\u00e9tate d\u2019ammonium 5 mM dans l\u2019eau<\/td>\n<\/tr>\n
Phase mobile B :<\/td>\nM\u00e9thanol<\/td>\n<\/tr>\n
Gradient<\/td>\nTemps (min)<\/td>\n%B<\/td>\n<\/tr>\n
\u00a0<\/td>\n0.00<\/td>\n20<\/td>\n<\/tr>\n
\u00a0<\/td>\n7.00<\/td>\n95<\/td>\n<\/tr>\n
\u00a0<\/td>\n9.00<\/td>\n95<\/td>\n<\/tr>\n
\u00a0<\/td>\n9.01<\/td>\n20<\/td>\n<\/tr>\n
\u00a0<\/td>\n11.0<\/td>\n20<\/td>\n<\/tr>\n
D\u00e9bit :<\/td>\n0.25 mL\/min<\/td>\n<\/tr>\n
Temps d’analyse :<\/td>\n11 min<\/td>\n<\/tr>\n
Volume d’injection :<\/td>\n10 \u00b5L<\/td>\n<\/tr>\n
Temp. de la colonne :<\/td>\n40 \u00b0C<\/td>\n<\/tr>\n
Mode d’ionisation :<\/td>\nESI-<\/td>\n<\/tr>\n
Tension du spray:<\/td>\n-2000<\/td>\n<\/tr>\n
Temp. de la source :<\/td>\n450 \u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n

<\/p>\n\n\n\n

Tableau I :<\/strong> Transitions MRM pour l\u2019analyse LC-MS\/MS simultan\u00e9e des PFAS r\u00e9glement\u00e9s, alternatifs et \u00e0 cha\u00eenes ultra-courtes.<\/p>\n\n\n\n

N\u00b0 du pic<\/strong><\/td>Compos\u00e9<\/strong><\/td>Ion Pr\u00e9curseur (parent)<\/strong><\/td>Ion Produit (fils)<\/strong><\/td>Standard Interne<\/strong><\/td><\/tr>
1<\/td>PFPrA<\/td>162.9<\/td>119.0<\/td>13<\/sup>C2<\/sub>-PFHxA<\/td><\/tr>
2<\/td>PFBA<\/td>212.8<\/td>169.0<\/td>13<\/sup>C2<\/sub>-PFOA<\/td><\/tr>
3<\/td>PFPrS<\/td>248.8<\/td>79.6<\/td>13<\/sup>C2<\/sub>-PFHxA<\/td><\/tr>
4<\/td>PFBS<\/td>298.8<\/td>79.9<\/td>13<\/sup>C2<\/sub>-PFHxA<\/td><\/tr>
5<\/td>13<\/sup>C2<\/sub>-PFHxA<\/td>314.9<\/td>270.0<\/td>\u2014<\/td><\/tr>
6<\/td>HFPO-DA<\/td>285.0<\/td>168.9<\/td>13<\/sup>C2<\/sub>-PFOA<\/td><\/tr>
7<\/td>ADONA<\/td>376.9<\/td>250.7<\/td>13<\/sup>C2<\/sub>-PFOA<\/td><\/tr>
8<\/td>PFOA<\/td>413.1<\/td>368.9<\/td>13<\/sup>C2<\/sub>-PFOA<\/td><\/tr>
9<\/td>13<\/sup>C2<\/sub>-PFOA<\/td>415.0<\/td>370.0<\/td>\u2014<\/td><\/tr>
10<\/td>PFOS<\/td>498.8<\/td>80.0<\/td>13<\/sup>C4<\/sub>-PFOS<\/td><\/tr>
11<\/td>13<\/sup>C4<\/sub>-PFOS<\/td>503.0<\/td>80.0<\/td>\u2014<\/td><\/tr>
12<\/td>9Cl-PF3ONS<\/td>530.8<\/td>350.7<\/td>13<\/sup>C4<\/sub>-PFOS<\/td><\/tr>
13<\/td>11Cl-PF3OUdS<\/td>630.7<\/td>451.0<\/td>13<\/sup>C4<\/sub>-PFOS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

R\u00e9sultats et Discussion<\/h2>\n\n\n\n

Performances chromatographiques<\/h4>\n\n\n\n

La forme des pics, leur r\u00e9tention et leur intensit\u00e9 \u00e9taient similaires dans les \u00e9chantillons d\u2019eau LC-MS et ceux d\u2019eaux de terrain. Il y avait une ligne de base plus \u00e9lev\u00e9e pour le signal du PFPrA dans tous les \u00e9chantillons d\u2019eaux de terrain, mais cela n\u2019a pas eu d\u2019impact n\u00e9gatif sur l\u2019int\u00e9gration et la quantification du PFPrA. Aucune interf\u00e9rence provenant de la matrice n\u2019a \u00e9t\u00e9 observ\u00e9e pour tous les \u00e9chantillons d\u2019eaux pr\u00e9par\u00e9s par dilution au \u00bd.<\/p>\n\n\n

Figure 1 :<\/strong>\u00a0Des pics tr\u00e8s fins et des r\u00e9tentions ad\u00e9quates ont \u00e9t\u00e9 obtenus pour l\u2019analyse LC-MS\/MS simultan\u00e9e des PFAS \u00e0 cha\u00eenes ultra-courtes avec les PFAS r\u00e9glement\u00e9s et alternatifs dans diff\u00e9rents types d\u2019eaux.<\/div>
\n\n

80 ppt Reagent Water Standard<\/strong><\/p>\n\n\n

\"Ultra-short<\/div>

LC_EV0555<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/L)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>\n
1.<\/td>Perfluoropropanoic acid (PFPrA)<\/td>2.74<\/td>80<\/td>162.9<\/td>119.0<\/td><\/tr>\n
2.<\/td>Perfluorobutanoic acid (PFBA)<\/a><\/td>4.69<\/td>80<\/td>212.8<\/td>169.0<\/td><\/tr>\n
3.<\/td>Perfluoropropanesulfonic acid (PFPrS)<\/td>5.13<\/td>80<\/td>248.8<\/td>79.6<\/td><\/tr>\n
4.<\/td>Perfluorobutanesulfonic acid (PFBS)<\/a><\/td>6.14<\/td>80<\/td>298.8<\/td>79.9<\/td><\/tr>\n
5.<\/td>Perfluoro-n-[1,2-13<\/sup>C2<\/sub>]hexanoic acid (13<\/sup>C2<\/sub>-PFHxA)<\/td>6.75<\/td>50<\/td>314.9<\/td>270.0<\/td><\/tr>\n
6.<\/td>Hexafluoropropylene oxide-dimer acid (HFPO-DA)<\/a><\/td>6.92<\/td>80<\/td>285.0<\/td>168.9<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/L)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>
7.<\/td>Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)<\/a><\/td>7.33<\/td>80<\/td>376.9<\/td>250.7<\/td><\/tr>\n
8.<\/td>Perfluorooctanoic acid (PFOA)<\/a><\/td>7.70<\/td>80<\/td>413.1<\/td>368.9<\/td><\/tr>\n
9.<\/td>Perfluoro-[1,2-13<\/sup>C2<\/sub>]octanoic acid (13<\/sup>C2<\/sub>-PFOA)<\/td>7.70<\/td>50<\/td>415.0<\/td>370.0<\/td><\/tr>\n
10.<\/td>Perfluorooctanesulfonic acid (PFOS)<\/a><\/td>8.01<\/td>80<\/td>498.8<\/td>80.0<\/td><\/tr>\n
11.<\/td>Perfluoro-[1,2,3,4-13<\/sup>C4<\/sub>]octanesulfonic acid (13<\/sup>C4<\/sub>-PFOS)<\/td>8.01<\/td>50<\/td>503.0<\/td>80.0<\/td><\/tr>\n
12.<\/td>9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)<\/a><\/td>8.15<\/td>80<\/td>530.8<\/td>350.7<\/td><\/tr>\n
13.<\/td>11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11Cl-PF3OUdS)<\/td>8.61<\/td>80<\/td>630.7<\/td>451.0<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n

80 ppt Fortified Groundwater Sample<\/strong><\/p>\n\n\n

\"Ultrashort<\/div>

LC_EV0556<\/p>

Conditions<\/h4>
Column<\/th>Raptor C18 (cat.# 9304A1E<\/a>)<\/td><\/tr>
Dimensions:<\/th>100 mm x 3 mm ID<\/td><\/tr>
Particle Size:<\/th>2.7 \u00b5m<\/td><\/tr>
Pore Size:<\/th>90 \u00c5<\/td><\/tr>
<\/td>
Temp.:<\/th>40 \u00b0C<\/td><\/tr>
Standard\/Sample<\/th><\/td><\/tr>
Conc.:<\/th>80 ppt<\/td><\/tr><\/td><\/tr>
Inj. Vol.:<\/th>10 \u00b5L <\/td><\/tr>
Mobile Phase<\/th><\/td><\/tr>
A:<\/th>Water, 5 mM ammonium acetate <\/td><\/tr>
B:<\/th>Methanol <\/td><\/tr>
<\/td>
Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
0.00<\/td>0.25<\/td>80<\/td>20<\/td><\/tr>
7.00<\/td>0.25<\/td>5<\/td>95<\/td><\/tr>
9.00<\/td>0.25<\/td>5<\/td>95<\/td><\/tr>
9.01<\/td>0.25<\/td>80<\/td>20<\/td><\/tr>
11.0<\/td>0.25<\/td>80<\/td>20<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table>
Detector<\/th>MS\/MS<\/td><\/tr>
Ion Mode:<\/th>ESI- <\/td><\/tr>
Mode:<\/th>MRM <\/td><\/tr>
Instrument<\/th>UHPLC<\/td><\/tr>
Sample Preparation<\/th>In a polypropylene vial, 250 \u00b5L of groundwater sample (fortified at 80 ppt) was mixed with 250 \u00b5L of 40:60 reagent water:methanol and 5 \u00b5L of internal standard solution (5 ng\/mL of 13<\/sup>C2<\/sub>-PFHxA, 13<\/sup>C2<\/sub>-PFOA, 13<\/sup>C4<\/sub>-PFOS in methanol). The vial was capped with a polyethylene cap prior to analysis. <\/td><\/tr>
Notes<\/th>A PFAS delay column (cat.# 27854<\/a>) was installed between the pump mixer and the injector.

<\/td><\/tr><\/table><\/div><\/div><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

Lin\u00e9arit\u00e9<\/h4>\n\n\n\n

Dans cette m\u00e9thode LC-MS\/MS pour l\u2019analyse simultan\u00e9e des PFAS \u00e0 cha\u00eenes ultra-courtes avec les PFAS r\u00e9glement\u00e9s et alternatifs, la gamme d\u2019\u00e9talonnage allait de 10 \u00e0 400 ppt pour le PFPrA et de 5 \u00e0 400 ppt pour tous les autres analytes. Tous les compos\u00e9s ont montr\u00e9 une lin\u00e9arit\u00e9 acceptable avec des valeurs de r \u2265 0.999 et des \u00e9carts relatifs <20%. Le 11Cl-PF3OUdS est le seul analyte qui a \u00e9t\u00e9 quantifi\u00e9 en utilisant une courbe d\u2019\u00e9talonnage \u00e0 r\u00e9gression quadratique (pond\u00e9r\u00e9e 1\/x). Tous les autres analytes ont \u00e9t\u00e9 quantifi\u00e9s en utilisant une r\u00e9gression lin\u00e9aire pond\u00e9r\u00e9e 1\/x (Figure 2).<\/p>\n\n\n

Figure 2 :<\/strong>\u00a0Courbes d\u2019\u00e9talonnage.<\/div>
\n
\n
\"\"<\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

Exactitude et Pr\u00e9cision<\/h4>\n\n\n\n

Les \u00e9chantillons d\u2019eaux non-dop\u00e9s ont montr\u00e9 (pr\u00e9sentaient ?) diff\u00e9rents niveaux de PFAS en C3, C4 et C8 mais sans PFPrS, ADONA, HFPO-DA, 9Cl-PF3ONS et 11Cl-PF3OUdS d\u00e9tectables (Tableau II). Pour le calcul de l\u2019exactitude (% rendement), la quantit\u00e9 mesur\u00e9e d\u2019analyte dans l\u2019\u00e9chantillon dop\u00e9 a \u00e9t\u00e9 ajust\u00e9e en fonction de la concentration de l\u2019\u00e9chantillon non-dop\u00e9. Le Tableau III montre les r\u00e9sultats d\u2019exactitude et de pr\u00e9cision calcul\u00e9s pour les trois lots de donn\u00e9es. L\u2019exactitude de la m\u00e9thode a \u00e9t\u00e9 d\u00e9montr\u00e9e par les valeurs des rendements qui se trouvaient \u00e0 +\/- 20% de la concentration nominale pour les deux niveaux de dopage ainsi que pour le standard pr\u00e9par\u00e9 dans l\u2019eau LC-MS ayant pour concentration la limite basse de quantification (LLOQ). L\u2019\u00e9cart-type relatif \u00e9tait <15%, indiquant une pr\u00e9cision acceptable de la m\u00e9thode.<\/p>\n\n\n\n

Tableau II :<\/strong> Concentrations des PFAS dans les \u00e9chantillons d\u2019eau non-dop\u00e9s.<\/p>\n\n\n\n

Concentration D\u00e9tect\u00e9e (ng\/L)<\/strong><\/td><\/tr>
 <\/td>PFPrA<\/strong><\/td>PFBA<\/strong><\/td>PFPrS<\/strong><\/td>PFBS<\/strong><\/td>HFPO-DA<\/strong><\/td>ADONA<\/strong><\/td>PFOA<\/strong><\/td>PFOS<\/strong><\/td>9Cl-PF3ONS<\/strong><\/td>11Cl-PF3OUdS<\/strong><\/td><\/tr>
Eau du robinet<\/td>ND<\/td>1.1<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td><\/tr>
Eau de rivi\u00e8re<\/td>ND<\/td>1.6<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td><\/tr>
Eaux souterraines<\/td>9.0<\/td>3.4<\/td>ND<\/td>2.6<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td>ND<\/td><\/tr>
Eaux us\u00e9es<\/td>11.7<\/td>10.6<\/td>ND<\/td>3.0<\/td>ND<\/td>ND<\/td>15.0<\/td>6.0<\/td>ND<\/td>ND<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Tableau III:<\/strong> Exactitude et Pr\u00e9cision.<\/p>\n\n\n\n

Conc. (ng\/L)<\/strong><\/td>Average %Recovery (%RSD)<\/strong><\/td><\/tr>
Eau du robinet<\/strong><\/td>Eau de rivi\u00e8re<\/strong><\/td>Eau souterraine<\/strong><\/td>Eaux us\u00e9es<\/strong><\/td>Eau r\u00e9active<\/strong><\/td><\/tr>
10*<\/strong><\/td>80<\/strong><\/td>10*<\/strong><\/td>80<\/strong><\/td>10*<\/strong><\/td>80<\/strong><\/td>10*<\/strong><\/td>80<\/strong><\/td>5**
(LLOQ)<\/strong><\/td><\/tr>
PFPrA<\/td>96.9 (11.0)<\/td>105 (3.91)<\/td>105 (6.57)<\/td>95.4 (6.84)<\/td>92.0 (9.54)<\/td>99.4 (7.40)<\/td>94.2 (5.29)<\/td>87.2 (8.18)<\/td>103
(10.9)<\/td><\/tr>
PFBA<\/td>99.3 (9.19)<\/td>108 (1.81)<\/td>108 (5.20)<\/td>110 (1.70)<\/td>104 (8.21)<\/td>108 (6.68)<\/td>108 (8.12)<\/td>97.1 (8.17)<\/td>97.9
(12.0)<\/td><\/tr>
PFPrS<\/td>100 (4.24)<\/td>107 (3.14)<\/td>103 (6.71)<\/td>105 (2.64)<\/td>105 (8.48)<\/td>109 (6.68)<\/td>109 (5.65)<\/td>103 (9.28)<\/td>99.1
(8.59)<\/td><\/tr>
PFBS<\/td>101 (5.20)<\/td>106 (1.84)<\/td>99.7 (7.54)<\/td>105 (2.10)<\/td>100 (6.57)<\/td>106 (2.82)<\/td>103 (1.93)<\/td>97.8 (5.85)<\/td>96.0
(8.75)<\/td><\/tr>
HFPO-DA<\/td>96.2 (7.86)<\/td>102 (4.64)<\/td>96.2 (4.99)<\/td>105 (3.94)<\/td>95.0 (3.59)<\/td>101 (8.92)<\/td>92.9 (4.87)<\/td>90.3 (7.77)<\/td>99.3
(8.54)<\/td><\/tr>
ADONA<\/td>101 (6.23)<\/td>106 (3.82)<\/td>97.6 (6.36)<\/td>106 (2.32)<\/td>98.4 (2.68)<\/td>105 (4.08)<\/td>98.2 (7.09)<\/td>98.2 (7.09)<\/td>102
(10.3)<\/td><\/tr>
PFOA<\/td>105 (8.65)<\/td>105 (3.70)<\/td>108 (12.1)<\/td>107 (3.63)<\/td>108 (9.66)<\/td>105 (5.26)<\/td>99.9 (10.5)<\/td>94.5 (7.24)<\/td>100
(9.05)<\/td><\/tr>
PFOS<\/td>99.3 (2.10)<\/td>108 (4.24)<\/td>112 (1.87)<\/td>107 (4.93)<\/td>101 (2.96)<\/td>102 (2.31)<\/td>104 (4.46)<\/td>98.3 (5.82)<\/td>94.3 (8.85)<\/td><\/tr>
9Cl-PF3ONS<\/td>95.6 (4.60)<\/td>106 (5.93)<\/td>105 (5.37)<\/td>110 (8.20)<\/td>97.2 (4.52)<\/td>107 (7.41)<\/td>101 (6.52)<\/td>99.8 (4.89)<\/td>98.8
(5.47)<\/td><\/tr>
11Cl-PF3OUdS<\/td>114 (8.78)<\/td>112 (8.91)<\/td>102 (15.0)<\/td>91.5 (2.34)<\/td>96.7 (5.99)<\/td>105 (15.2)<\/td>115 (2.67)<\/td>103 (8.45)<\/td>105
(8.04)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*20 ng\/L for PFPrA
**10 ng\/L for PFPrA<\/p>\n\n\n\n

Conclusion<\/h2>\n\n\n\n

Une m\u00e9thode LC-MS\/MS robuste, pour quantifier une large gamme de PFAS de longueurs de cha\u00eenes et structures diff\u00e9rentes, et utilisant une approche simple d\u2019injection directe, a \u00e9t\u00e9 \u00e9valu\u00e9e avec succ\u00e8s dans diff\u00e9rents types d\u2019eaux. \u00c0 l\u2019aide d\u2019une colonne Raptor C18 3.0 x 100 mm (2.7 \u00b5m) et d\u2019une “colonne-retard” PFAS, la m\u00e9thode analytique s\u2019est av\u00e9r\u00e9e rapide, robuste et sensible avec une exactitude et une pr\u00e9cision acceptables. Cette m\u00e9thode convient aux laboratoires devant analyser une liste \u00e9tendue de PFAS, incluant les PFAS \u00e0 cha\u00eenes ultra-courtes, dans les eaux potables et non-potables.<\/p>\n\n\n\n

<\/div>\n\n\n\n

References<\/h2>\n\n\n\n
    \n
  1. S. Taniyasu, K. Kannan, L.W.Y. Yeung, K.Y. Kwok, P.K.S Lam, N. Yamashita, Analysis of trifluoroacetic acid and other short-chain perfluorinated acids (C2-C4) in precipitation by liquid chromatography-tandem mass spectrometry: comparison to patterns of long-chain perfluorinated acids (C5-C18), Anal. Chim. Acta. 619 (2008) 221-230.<\/li>\n\n\n\n
  2. J. Janda, K. Nodler, H-J. Brauch, C. Zwiener, F.T. Lange, Robust trace analysis of polar (C2-C8) perfluorinated carboxylic acids by liquid chromatography-tandem mass spectrometry: method development and application to surface water, groundwater, and drinking water, Environ. Sci. Pollut.R. 26 (2018) 7326-7336.<\/li>\n\n\n\n
  3. K.Y. Kwok, S. Taniyasu, L.W.Y. Yeung, M.B. Murphy, P.K.S. Lam, Y. Horii, K. Kannan, G. Petrick, R.K. Sinha, N. Yamashita, Flux of perfluorinated chemicals through wet deposition in Japan, the United States, and other countries, Environ. Sci. Technol. 44 (2010) 7043-7049.<\/li>\n\n\n\n
  4. K.A. Barzen-Hanson, J.A. Field, Discovery and implications of C2 and C3 perfluoroalkyl sulfonates in aqueous film-forming foams and groundwater, Environ. Sci. Technol. Lett. 5 (2015) 95-99.<\/li>\n<\/ol>\n\n\n\n
    <\/div>\n\n\n
    \n

    Products Mentioned<\/h3>\n
    \n
    \n
    \n
    \n Catalog No. 27854 <\/a> <\/div>\n
    \n
    \u201cColonne-retard\u201d PFAS HPLC, 5 \u00b5m, 50 x 2.1 mm<\/div>\n <\/div>\n
    \n Voir le produit<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 9304A1E <\/a> <\/div>\n
    \n
    Colonne Raptor C18 2.7\u00b5m, 100 x 3.0 mm<\/div>\n <\/div>\n
    \n Voir le produit<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Une m\u00e9thode LC-MS\/MS simple, par injection directe, a \u00e9t\u00e9 d\u00e9velopp\u00e9e et \u00e9valu\u00e9e pour l\u2019analyse simultan\u00e9e des PFAS (Substances Per- et PolyFluoroAlkyl\u00e9es) r\u00e9glement\u00e9s, alternatifs et \u00e0 cha\u00eenes ultra-courtes dans diff\u00e9rents types d\u2019eaux. Cette m\u00e9thode est recommand\u00e9e pour les laboratoires souhaitant n\u2019utiliser qu\u2019une seule proc\u00e9dure pour analyser ces trois cat\u00e9gories de PFAS dans les eaux potables et non-potables.<\/p>\n","protected":false},"author":11,"featured_media":6529,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[782],"tags":[],"industries-application":[2212,2208],"post-badge":[],"resource-type":[],"product-library":[2463,2477,2445,2447,2474],"resource-technique":[2318,2362],"ppma_author":[414],"class_list":["post-43400","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-notes-dapplication","industries-application-analyse-de-pfas","industries-application-environnement","product-library-colonnes-lc","product-library-flacons-accessoires","product-library-produits-pour-la-chromatographie-en-phase-liquide","product-library-produits-pour-la-preparation-dechantillons","product-library-spe-sle-fr","resource-technique-chromatographie-en-phase-liquide","resource-technique-ms-ms-fr"],"acf":[],"taxonomy_info":{"category":[{"value":782,"label":"Notes 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