{"id":43411,"date":"2020-10-14T14:30:00","date_gmt":"2020-10-14T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/rapid-perfluorinated-alkyl-acid-analysis-by-lc-msms-increases-sample-throughput\/"},"modified":"2026-01-28T16:35:51","modified_gmt":"2026-01-28T16:35:51","slug":"rapid-perfluorinated-alkyl-acid-analysis-by-lc-msms-increases-sample-throughput","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles\/evar2397\/rapid-perfluorinated-alkyl-acid-analysis-by-lc-msms-increases-sample-throughput","title":{"rendered":"Rapid Perfluorinated Alkyl Acid Analysis by LC-MS\/MS Increases Sample Throughput"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Raptor C18 SPP 5 \u00b5m core-shell silica particle columns offer excellent resolution for fluorochemicals with short total cycle times.<\/li>\n\n\n\n<li>Meets the requirements for EPA Method 537 and ISO 25101.<\/li>\n\n\n\n<li>Unique, robust Raptor C18 column design increases instrument uptime.<\/li>\n<\/ul>\n\n\n\n<p>Perfluorinated alkyl acids are man-made fluorochemicals used as surface-active agents in the manufacture of a variety of products, such as firefighting foams, coating additives, textiles, and cleaning products. They have been detected in the environment globally and are used in very large quantities around the world. These fluorochemicals are extremely persistent and resistant to typical environmental degradation processes. As a result, they are widely distributed across the higher trophic levels and are found in soil, air, groundwater, municipal refuse, and landfill leachates. The toxicity, mobility, and bioaccumulation potential of perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), in particular, pose potential adverse effects for the environment and human health.<\/p>\n\n\n\n<p>Due to exposure risk and the likelihood of these detrimental impacts, PFOS and PFOA have both come under regulatory scrutiny, and many countries have restricted their use and are establishing threshold levels in drinking water and other matrices. In Directive 2013\/39\/EU (an amendment to the Water Framework Directive), PFOS is named as a \u201cPriority Substance\u201d with very challenging environmental quality standards. EU Directive 2006\/122\/ECOF now prohibits the manufacture and use of PFOS in the European Union, and this directive also considers PFOA to present a risk profile that is comparable to PFOS. Perfluorinated alkyl substances (PFAS) in general are not currently regulated in the United States, but the U.S. EPA has issued a Health Advisory for PFOS and PFOA, and most U.S. states and cities monitor for the presence of PFAS in drinking water.<\/p>\n\n\n\n<p>Perfluorinated alkyl acid analysis can be challenging because these compounds are chemically different from most other environmental contaminants. They are difficult to quantify because some are more volatile than others, and they also tend to be more hydrophilic and somewhat reactive. In addition, fluorochemicals are present in polytetrafluoroethylene (PTFE) materials, so excluding the use of any PTFE labware throughout the sampling and analytical processes (including HPLC solvent inlet tubing) is essential for accurate analysis. Typically, perfluorinated alkyl acids are analyzed by LC-MS\/MS methods, such as EPA Method 537, ISO 25101, DIN 38407-42, ASTM D7979, or ASTM D7968, but long analysis times can significantly limit sample throughput. These methods all provide example chromatographic conditions, with analysis times ranging anywhere from 15-27 minutes in length, depending on the standard. However, each method also permits flexibility when defining analytical parameters and choosing a column, which allows laboratories to optimize their conditions to meet method requirements as efficiently as possible.<\/p>\n\n\n\n<p>Taking advantage of the faster, more efficient separations that are possible with superficially porous particle (SPP) columns, the method presented here offers a fast analysis with sufficient resolution to permit adequate MS dwell time for all compounds within a specific retention time window. As shown in Figure 1, all target perfluorinated alkyl acids were analyzed on a Raptor C18 column with a total cycle time of 9 minutes without sacrificing peak resolution or selectivity. All analytes elute as highly symmetrical peaks that can be accurately identified, integrated, and quantified by MS\/MS. This method also easily resolves PFHxS and PFOS branched isomers for standards requiring their summation with the unbranched compounds as a part of reporting (e.g., EPA Method 537).<\/p>\n\n\n\n<p>If PFOA and PFOS are the only target fluorochemicals, the chromatographic conditions can be further optimized. The chromatogram in Figure 2 demonstrates that a fast, &lt;2-minute separation with a total cycle time of just 4.5 minutes can be achieved using a 2.1 x 100 mm Raptor C18 column. Alternatively, a shorter column (2.1 x 50 mm) or a column with smaller particles (e.g., 2.7 \u00b5m particles) can be used for an even faster analysis.<\/p>\n\n\n\n<p>Whether labs conducting perfluorinated alkyl acid analysis by LC use longer target analyte lists or focus just on PFOA and PFOS, the excellent peak shapes and separations achieved here result in consistent, accurate quantification with much shorter analysis times. By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Decrease analysis time for comprehensive perfluorinated alkyl acid analysis using a 5 \u00b5m Raptor C18 column and these instrument conditions.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0548.png\" alt=\"Perfluorinated Alkyl Acids on Raptor C18 by LC-MS\/MS for EPA Method 537\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0548<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanesulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanesulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">10<\/td><td class=\"oth\">299.10<\/td><td class=\"oth\">79.99<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]hexanoic acid (<sup>13<\/sup>C-PFHxA)<\/td><td class=\"oth\">2.92<\/td><td class=\"oth\">10<\/td><td class=\"oth\">315.13<\/td><td class=\"oth\">270.13<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">2.93<\/td><td class=\"oth\">10<\/td><td class=\"oth\">313.10<\/td><td class=\"oth\">269.12<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroheptanoic acid (PFHpA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-85-9\/Perfluoroheptanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroheptanoic acid (PFHpA)<\/a><\/td><td class=\"oth\">4.00<\/td><td class=\"oth\">5<\/td><td class=\"oth\">363.16<\/td><td class=\"oth\">319.09<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanesulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">4.14<\/td><td class=\"oth\">10<\/td><td class=\"oth\">399.13<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoro-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]octanoic acid (<sup>13<\/sup>C-PFOA)<\/td><td class=\"oth\">4.83<\/td><td class=\"oth\">5<\/td><td class=\"oth\">415.13<\/td><td class=\"oth\">370.10<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">4.83<\/td><td class=\"oth\">5<\/td><td class=\"oth\">413.16<\/td><td class=\"oth\">369.10<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononanoic acid (PFNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-95-1\/Perfluorononanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononanoic acid (PFNA)<\/a><\/td><td class=\"oth\">5.50<\/td><td class=\"oth\">5<\/td><td class=\"oth\">463.16<\/td><td class=\"oth\">419.19<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\">Perfluoro-1-[1,2,3,4-<sup>13<\/sup>C<sub>4<\/sub>]octanesulfonic acid (<sup>13<\/sup>C-PFOS)<\/td><td class=\"oth\">5.54<\/td><td class=\"oth\">5<\/td><td class=\"oth\">503.13<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">5.54<\/td><td class=\"oth\">10<\/td><td class=\"oth\">499.17<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]decanoic acid (<sup>13<\/sup>C-PFDA)<\/td><td class=\"oth\">6.06<\/td><td class=\"oth\">5<\/td><td class=\"oth\">515.17<\/td><td class=\"oth\">470.17<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecanoic acid (PFDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-76-2\/Perfluorodecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecanoic acid (PFDA)<\/a><\/td><td class=\"oth\">6.07<\/td><td class=\"oth\">5<\/td><td class=\"oth\">513.17<\/td><td class=\"oth\">469.16<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)<\/td><td class=\"oth\">6.28<\/td><td class=\"oth\">5<\/td><td class=\"oth\">573.23<\/td><td class=\"oth\">419.15<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2355-31-9\/N-methyl perfluorooctanesulfonamidoacetic acid\" target=\"_blank\" rel=\"noopener\">N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)<\/a><\/td><td class=\"oth\">6.28<\/td><td class=\"oth\">5<\/td><td class=\"oth\">570.20<\/td><td class=\"oth\">419.17<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\">N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)<\/td><td class=\"oth\">6.52<\/td><td class=\"oth\">5<\/td><td class=\"oth\">589.27<\/td><td class=\"oth\">419.11<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2991-50-6\/N-ethyl perfluorooctanesulfonamidoacetic acid\" target=\"_blank\" rel=\"noopener\">N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)<\/a><\/td><td class=\"oth\">6.52<\/td><td class=\"oth\">5<\/td><td class=\"oth\">584.20<\/td><td class=\"oth\">419.18<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroundecanoic acid (PFUnA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2058-94-8\/Perfluoroundecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroundecanoic acid (PFUnA)<\/a><\/td><td class=\"oth\">6.56<\/td><td class=\"oth\">5<\/td><td class=\"oth\">563.23<\/td><td class=\"oth\">519.24<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorododecanoic acid (PFDoA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-55-1\/Perfluorododecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorododecanoic acid (PFDoA)<\/a><\/td><td class=\"oth\">6.98<\/td><td class=\"oth\">5<\/td><td class=\"oth\">613.23<\/td><td class=\"oth\">569.19<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorotridecanoic acid (PFTrDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72629-94-8\/Perfluorotridecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorotridecanoic acid (PFTrDA)<\/a><\/td><td class=\"oth\">7.35<\/td><td class=\"oth\">5<\/td><td class=\"oth\">663.23<\/td><td class=\"oth\">619.21<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorotetradecanoic acid (PFTA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/376-06-7\/Perfluorotetradecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorotetradecanoic acid (PFTA)<\/a><\/td><td class=\"oth\">7.67<\/td><td class=\"oth\">5<\/td><td class=\"oth\">713.23<\/td><td class=\"oth\">669.23<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304552?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0548\" rel=\"noopener\">cat.# 9304552<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>96:4 Methanol:water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5\u201310 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>5 mM ammonium acetate in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>8.00<\/td><td>0.4<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>8.01<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0548.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<p><strong>Figure 2:<\/strong>&nbsp;Separate PFOS and PFOA to baseline in a fast, &lt;2 min analysis (4.5 min total cycle time).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0536.png\" alt=\"Perfluorooctanoic Acid (PFOA) and Perfluorooctanesulfonic Acid (PFOS) on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0536<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Perfluoro-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]octanoic acid (<sup>13<\/sup>C-PFOA)<\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">10<\/td><td class=\"oth\">414.8<\/td><td class=\"oth\">369.8<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">5<\/td><td class=\"oth\">412.7<\/td><td class=\"oth\">368.8<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">Perfluoro-1-[1,2,3,4-<sup>13<\/sup>C<sub>4<\/sub>]octanesulfonic acid (<sup>13<\/sup>C-PFOS)<\/td><td class=\"oth\">1.65<\/td><td class=\"oth\">10<\/td><td class=\"oth\">502.7<\/td><td class=\"oth\">79.9<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">1.65<\/td><td class=\"oth\">5<\/td><td class=\"oth\">498.7<\/td><td class=\"oth\">79.9<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304512?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0536\" rel=\"noopener\">cat.# 9304512<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:methanol (50:50)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5-10 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>5 mM ammonium acetate in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>2.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>2.51<\/td><td>0.4<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>4.50<\/td><td>0.4<\/td><td>40<\/td><td>60<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0536.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Whether labs conducting perfluorinated alkyl acidACA analysis by LC use longer target analyte lists or focus just on PFOA and PFOS, the excellent peak shapes and separations achieved here result in consistent, accurate quantification in much faster analysis times. By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.<\/p>\n","protected":false},"author":46,"featured_media":6589,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2231,2232,2230,2668,2235],"post-badge":[],"resource-type":[],"product-library":[2499,2481],"resource-technique":[2326,2328,2363],"hf_cat_post":[650],"ppma_author":[578],"class_list":["post-43411","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","industries-application-luftprufung","industries-application-trinkwasser-abwasseruntersuchungen","industries-application-umweltbereich","industries-application-semivolatiles","industries-application-testung-von-boden-sediment-organismen","product-library-lc-saulen","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie","resource-technique-massenspektrometrie-ms","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2231,"label":"Luftpr\u00fcfung"},{"value":2232,"label":"Trinkwasser- und Abwasseruntersuchungen"},{"value":2230,"label":"Umweltbereich"},{"value":2668,"label":"Semivolatiles"},{"value":2235,"label":"Testung von Boden, Sediment, Organismen"}],"product-library":[{"value":2499,"label":"LC S\u00e4ulen"},{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"},{"value":2328,"label":"Massenspektrometrie (MS)"},{"value":2363,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-EVAR2397-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Shun-Hsin Liang, 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