{"id":43415,"date":"2020-10-20T14:30:00","date_gmt":"2020-10-20T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/comment-saffranchir-des-pfas-provenant-dun-systeme-analytique-en-utilisant-une-colonne-retard\/"},"modified":"2026-04-15T16:31:33","modified_gmt":"2026-04-15T16:31:33","slug":"comment-saffranchir-des-pfas-provenant-dun-systeme-analytique-en-utilisant-une-colonne-retard","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-fr\/evar3001-fr\/comment-saffranchir-des-pfas-provenant-dun-systeme-analytique-en-utilisant-une-colonne-retard","title":{"rendered":"Comment s\u2019affranchir des PFAS provenant d\u2019un syst\u00e8me analytique, en utilisant une &#8220;colonne-retard&#8221;"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>La &#8220;colonne-retard&#8221; retient les PFAS contaminants provenant de l\u2019appareillage et retarde leur \u00e9lution pour \u00e9viter toute interf\u00e9rence avec les PFAS de l\u2019\u00e9chantillon.<\/li>\n\n\n\n<li>Elle facilite l\u2019identification et la quantification exacte des PFAS pr\u00e9sents au niveau de traces dans l\u2019\u00e9chantillon.<\/li>\n\n\n\n<li>Elle retient fortement les PFAS contaminants m\u00eame durant un temps d\u2019\u00e9quilibrage prolong\u00e9.<\/li>\n<\/ul>\n\n\n\n<p>Les PFAS (Poly- et perfluoroalkyl substances) font partie des polluants environnementaux les plus contr\u00f4l\u00e9s dans le Monde. Du fait de leur utilisation croissante et de leur r\u00e9manence dans l\u2019environnement, ils constituent un probl\u00e8me s\u00e9rieux \u00e0 l\u2019\u00e9chelle mondiale. Inquiets des risques que repr\u00e9sentent pour la sant\u00e9 ces contaminants, des scientifiques ont contr\u00f4l\u00e9 leur pr\u00e9sence sur l\u2019ensemble du Globe. Des \u00e9tudes men\u00e9es sur les pingouins [1] du P\u00f4le Sud jusqu\u2019aux ours du P\u00f4le Nord [2] et en divers autres points de la Terre, ont mis en \u00e9vidence l\u2019existence d\u2019une contamination de l\u2019environnement par les PFAS d\u2019un p\u00f4le \u00e0 l\u2019autre [3,4]. Il a \u00e9t\u00e9 aussi d\u00e9couvert que certains de ces compos\u00e9s sont pr\u00e9sents \u00e0 l\u2019int\u00e9rieur m\u00eame des appareils utilis\u00e9s pour l\u2019analyse des PFAS dans les \u00e9chantillons environnementaux. Ces PFAS contaminants peuvent fausser les analyses de traces de PFAS g\u00e9n\u00e9ralement recherch\u00e9es au stade de la ppt voire moins. Les PFAS pr\u00e9sents dans les syst\u00e8mes analytiques peuvent provenir des \u00e9l\u00e9ments contenant des fluoropolym\u00e8res comme le PTFE et qui migrent ensuite dans les phases mobiles. L\u2019utilisation de tubes et de r\u00e9cipients exempts de produits fluor\u00e9s et le remplacement d\u2019\u00e9l\u00e9ments en PEEK par d\u2019autres en acier inoxydable, peut diminuer cette pollution intrins\u00e8que. Ces mesures ne suffisent pas \u00e0 \u00e9liminer suffisamment cette contamination pour qu\u2019elle ne fausse pas les analyses de traces de PFAS. Une autre solution est donc indispensable.<\/p>\n\n\n\n<p>Dans le cas d\u2019un syst\u00e8me LC \u00e0 configuration classique, les PFAS provenant de l\u2019appareillage sont transport\u00e9s par la phase mobile jusqu\u2019\u00e0 la colonne analytique o\u00f9 ils sont retenus entre deux injections. Cette \u00e9tude montre qu\u2019une deuxi\u00e8me colonne dite &#8220;colonne-retard&#8221; plac\u00e9e sur le circuit de la phase mobile juste avant l\u2019injecteur, peut efficacement pi\u00e9ger les PFAS contaminants avant qu\u2019ils n\u2019atteignent la colonne analytique (Figure 1). Ainsi, seuls les PFAS pr\u00e9sents dans l\u2019\u00e9chantillon inject\u00e9 sont concentr\u00e9s en t\u00eate de la colonne analytique pour \u00eatre ensuite normalement \u00e9lu\u00e9s au cours d\u2019un gradient. Les PFAS contaminants (provenant du syst\u00e8me analytique) retenus dans la &#8220;colonne-retard&#8221; durant le temps d\u2019\u00e9quilibrage, sont \u00e9galement \u00e9lu\u00e9s \u00e0 travers la colonne analytique mais sous l\u2019effet de la &#8220;colonne-retard&#8221;, seulement apr\u00e8s les PFAS de l\u2019\u00e9chantillon. De plus, les PFAS contaminants \u00e9tant pr\u00e9sents dans le flux permanent de phase mobile et ne provenant pas d\u2019une injection, ils ne sont pas concentr\u00e9s en t\u00eate de colonne analytique. L\u2019int\u00e9r\u00eat \u00e9vident de la &#8220;colonne-retard&#8221; est qu\u2019elle permet de diff\u00e9rencier les PFAS contaminants des PFAS de l\u2019\u00e9chantillon pour une quantification exacte de ces derniers.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1 :<\/strong>\u00a0Positionnement de la &#8220;colonne-retard&#8221; pour l\u2019analyse des PFAS.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"607\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-EVAR3001-01-607x1024.jpg\" alt=\"\" class=\"wp-image-19512\" style=\"width:485px;height:auto\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-607x1024.jpg 607w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-178x300.jpg 178w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-768x1296.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-910x1536.jpg 910w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-1214x2048.jpg 1214w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3001-01-scaled.jpg 1517w\" sizes=\"auto, (max-width: 607px) 100vw, 607px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<p>La Figure 2 montre comment une contamination du syst\u00e8me peut fausser une analyse de PFAS. L\u2019ensemble analytique (sans &#8220;colonne-retard&#8221;) est ici \u00e9quilibr\u00e9 durant 10 minutes pour laisser les PFAS contaminants se concentrer en t\u00eate de colonne avant qu\u2019une injection \u00e0 blanc ne soit faite. Ce &#8220;blanc&#8221; laisse appara\u00eetre 5 compos\u00e9s-cibles potentiels parmi les contaminants. La nature des PFAS contaminants et leur concentration d\u00e9pendent du syst\u00e8me (marque et mod\u00e8le de l\u2019appareil, mat\u00e9riaux des tubes et des raccords), de la qualit\u00e9 de la phase mobile et des conditions analytiques.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2\u00a0<\/strong>: Pr\u00e9sence de PFAS contaminants dans un &#8220;blanc&#8221; r\u00e9alis\u00e9 sans &#8220;colonne-retard&#8221;.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0549.png\" alt=\"Background Perfluoroalkyl Substances in Waters ACQUITY UPLC I-Class Xevo TQ-S System\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0549<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">3.75<\/td><td class=\"oth\">413.2<\/td><td class=\"oth\">369.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononanoic acid (PFNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-95-1\/Perfluorononanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononanoic acid (PFNA)<\/a><\/td><td class=\"oth\">4.15<\/td><td class=\"oth\">463.2<\/td><td class=\"oth\">419.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecanoic acid (PFDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-76-2\/Perfluorodecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecanoic acid (PFDA)<\/a><\/td><td class=\"oth\">4.48<\/td><td class=\"oth\">513.2<\/td><td class=\"oth\">469.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroundecanoic acid (PFUnA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2058-94-8\/Perfluoroundecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroundecanoic acid (PFUnA)<\/a><\/td><td class=\"oth\">4.77<\/td><td class=\"oth\">563.2<\/td><td class=\"oth\">519.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorododecanoic acid (PFDoA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-55-1\/Perfluorododecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorododecanoic acid (PFDoA)<\/a><\/td><td class=\"oth\">5.02<\/td><td class=\"oth\">613.2<\/td><td class=\"oth\">569.2<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0549\" rel=\"noopener\">cat.# 9304252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Blank injection without delay column after 10 minutes of equilibration.<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>80:20 Water:methanol<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 5mM ammonium acetate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>6.00<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.51<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>8.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0549.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p>L\u2019utilisation d\u2019une &#8220;colonne-retard&#8221; est un moyen efficace de retenir les PFAS contaminants et de pr\u00e9venir ainsi leur co\u00e9lution avec les PFAS de l\u2019\u00e9chantillon. L\u2019exp\u00e9rience d\u00e9crite en Figure 2 est r\u00e9p\u00e9t\u00e9e apr\u00e8s l\u2019installation d\u2019une &#8220;colonne-retard&#8221;. Cette fois et apr\u00e8s une nouvelle injection \u00e0 blanc, aucun pic n\u2019est observ\u00e9 aux temps de r\u00e9tention correspondant aux PFAS contaminants pr\u00e9c\u00e9demment rep\u00e9r\u00e9s, cela malgr\u00e9 des temps d\u2019\u00e9quilibrage relativement longs. En revanche, les PFAS contaminants issus du syst\u00e8me analytique sont \u00e9lu\u00e9s sous forme d\u2019une large bande&nbsp;<em>apr\u00e8s<\/em>&nbsp;l\u2019\u00e9lution du compos\u00e9 d\u2019int\u00e9r\u00eat. Cette large bande s\u2019explique par le fait que les PFAS contaminants sont retenus sur la &#8220;colonne-retard&#8221; durant tout le temps de l\u2019\u00e9quilibrage. Leur \u00e9lution ne produit donc pas un pic bien d\u00e9fini mais une zone avec une ligne de base l\u00e9g\u00e8rement haute (Figure 4).<\/p>\n\n\n\n<p>La &#8220;colonne-retard&#8221; augmente la distance du circuit entre le m\u00e9langeur et l\u2019injecteur. Le temps de r\u00e9tention du compos\u00e9 d\u2019int\u00e9r\u00eat est donc l\u00e9g\u00e8rement plus long car il faut plus de temps \u00e0 la phase mobile pour atteindre la colonne analytique au cours d\u2019une analyse avec gradient (Figure 3). L\u2019int\u00e9r\u00eat d\u2019une &#8220;colonne-retard&#8221; est mis en \u00e9vidence en Figure 4 : les temps de r\u00e9tention des compos\u00e9s-cibles sont hors de la plage d\u2019\u00e9lution des PFAS contaminants provenant du syst\u00e8me. Il ne peut donc y avoir d\u2019interf\u00e9rence n\u00e9faste durant l\u2019analyse. A noter que les plages d\u2019\u00e9lution des PFAS contaminants du chromatogramme en Figure 4 sont \u00e9tablies en jouant sur la dur\u00e9e de l\u2019\u00e9quilibrage tout en surveillant l\u2019\u00e9l\u00e9vation de la ligne de base caus\u00e9e par l\u2019\u00e9lution des PFAS contaminants.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3 :<\/strong>\u00a0Une solution-\u00e9talon est utilis\u00e9e pour d\u00e9terminer les temps de r\u00e9tention attendus pour les PFAS de l\u2019\u00e9chantillon avec un syst\u00e8me \u00e9quip\u00e9 d\u2019une &#8220;colonne-retard&#8221;.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0554.png\" alt=\"Injection of Five Perfluoroalkyl Substances on Raptor C18 and Waters ACQUITY UPLC I-Class Xevo TQ-S System with PFAS Delay Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0554<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">3.99<\/td><td class=\"oth\">0.1<\/td><td class=\"oth\">413.2<\/td><td class=\"oth\">369.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononanoic acid (PFNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-95-1\/Perfluorononanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononanoic acid (PFNA)<\/a><\/td><td class=\"oth\">4.37<\/td><td class=\"oth\">0.1<\/td><td class=\"oth\">463.2<\/td><td class=\"oth\">419.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecanoic acid (PFDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-76-2\/Perfluorodecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecanoic acid (PFDA)<\/a><\/td><td class=\"oth\">4.70<\/td><td class=\"oth\">0.1<\/td><td class=\"oth\">513.2<\/td><td class=\"oth\">469.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroundecanoic acid (PFUnA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2058-94-8\/Perfluoroundecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroundecanoic acid (PFUnA)<\/a><\/td><td class=\"oth\">4.98<\/td><td class=\"oth\">0.1<\/td><td class=\"oth\">563.2<\/td><td class=\"oth\">519.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorododecanoic acid (PFDoA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-55-1\/Perfluorododecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorododecanoic acid (PFDoA)<\/a><\/td><td class=\"oth\">5.23<\/td><td class=\"oth\">0.1<\/td><td class=\"oth\">613.2<\/td><td class=\"oth\">569.2<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0554\" rel=\"noopener\">cat.# 9304252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>80:20 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>0.1 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 5 mM ammonium acetate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>6.00<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.51<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>8.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>A PFAS delay column (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/27854?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0554\" rel=\"noopener\">cat.# 27854<\/a>) was installed before the injector.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0554.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4 :<\/strong>\u00a0En repoussant l\u2019\u00e9lution des PFAS contaminants provenant du syst\u00e8me apr\u00e8s celle des PFAS de l\u2019\u00e9chantillons, la &#8220;colonne-retard&#8221; \u00e9vite toute interf\u00e9rence des uns avec les autres. Aucun pic n\u2019est d\u00e9tect\u00e9 aux temps de r\u00e9tention des PFAS de l\u2019\u00e9chantillon. Les fl\u00e8ches indiquent uniquement les temps de r\u00e9tention attendus.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0550.png\" alt=\"Delayed Background Perfluoroalkyl Substances in Waters ACQUITY UPLC I-Class Xevo TQ-S System\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0550<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">413.2<\/td><td class=\"oth\">369.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononanoic acid (PFNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-95-1\/Perfluorononanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononanoic acid (PFNA)<\/a><\/td><td class=\"oth\">463.2<\/td><td class=\"oth\">419.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecanoic acid (PFDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-76-2\/Perfluorodecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecanoic acid (PFDA)<\/a><\/td><td class=\"oth\">513.2<\/td><td class=\"oth\">469.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroundecanoic acid (PFUnA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2058-94-8\/Perfluoroundecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroundecanoic acid (PFUnA)<\/a><\/td><td class=\"oth\">563.2<\/td><td class=\"oth\">519.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorododecanoic acid (PFDoA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-55-1\/Perfluorododecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorododecanoic acid (PFDoA)<\/a><\/td><td class=\"oth\">613.2<\/td><td class=\"oth\">569.2<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0550\" rel=\"noopener\">cat.# 9304252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Blank injection with delay column (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/27854?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0550\" rel=\"noopener\">cat.# 27854<\/a>) after 10 minutes of equilibration.<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>80:20 Water:methanol<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 5mM ammonium acetate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>6.00<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.51<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>8.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0550.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p>En r\u00e9sum\u00e9, l\u2019utilisation d\u2019une &#8220;colonne-retard&#8221; permet d\u2019\u00e9viter que les PFAS contaminants issus du syst\u00e8me analytique ne faussent l\u2019analyse de PFAS pr\u00e9sents dans l\u2019\u00e9chantillon. La &#8220;colonne-retard&#8221; a pour effet de retarder l\u2019\u00e9lution des PFAS contaminants jusqu\u2019\u00e0 l\u2019injection de l\u2019\u00e9chantillon contenant les PFAS et le d\u00e9but du gradient. L\u2019\u00e9cart de temps entre l\u2019\u00e9lution des PFAS de l\u2019\u00e9chantillon et celle des PFAS contaminants est suffisamment grand pour \u00e9viter toute interf\u00e9rence et permettre ainsi une quantification exacte des premiers.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">R\u00e9f\u00e9rences<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>A. Schiavone, S. Corsolini, K. Kannan, L. Tao, W. Trivelpiece, et. al. Perfluorinated contaminants in fur seal pups and penguin eggs from South Shetland, Antarctica, Sci. Total., 407 (12) (2009) 3899-904.&nbsp;<a href=\"https:\/\/doi.org\/10.1016\/j.scitotenv.2008.12.058\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.scitotenv.2008.12.058<\/a><\/li>\n\n\n\n<li>S. Tartu, S. Bourgeon, J. Aars, M. Andersen, et al. Diet and metabolic state are the main factors determining concentrations of perfluoroalkyl substances in female polar bears from Svalbard, Environ. Pollut., 229 (2017) 146-158.&nbsp;<a href=\"https:\/\/doi.org\/10.1016\/j.envpol.2017.04.100\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.envpol.2017.04.100<\/a><\/li>\n\n\n\n<li>K. Rankin, S. Mabury, T, Jenkins, J. Washington, A North American and global survey of perfluoroalkyl substances in surface soils: Distribution patterns and mode of occurrence, Chemosphere, 161 (2016) 333-41.&nbsp;<a href=\"https:\/\/doi.org\/10.1016\/j.chemosphere.2016.06.109\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.chemosphere.2016.06.109<\/a><\/li>\n\n\n\n<li>B. Gonzalez-Gaya, J. Dachs, J Roscales, G. Cabellero, B. Jiminez, Perfluoroalkylated Substances in the Global Tropical and Subtropical Surface Oceans, Environ. Sci. Technol., 48 (22) (2014) 13076-84.&nbsp;<a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/es503490z\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/es503490z<\/a><\/li>\n<\/ol>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9304252\">                                Catalog No. 9304252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonne Raptor C18 1.8\u00b5m, 50 x 2.1 mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9304252\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/27854\">                                Catalog No. 27854                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">\u201cColonne-retard\u201d PFAS HPLC, 5 \u00b5m, 50 x 2.1 mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/27854\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>La colonne-retard&#8221; retient efficacement les PFAS contaminants provenant de l\u2019appareillage et retarde leur \u00e9lution pour \u00e9viter toute interf\u00e9rence avec les PFAS de l\u2019\u00e9chantillon. Cet article explique comment une &#8220;colonne-retard&#8221; PFAS facilite l\u2019identification et la quantification exacte des PFAS pr\u00e9sents au niveau de traces dans l\u2019\u00e9chantillon.&#8221;<\/p>\n","protected":false},"author":11,"featured_media":6595,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[2782],"industries-application":[2212,2208],"post-badge":[],"resource-type":[],"product-library":[2463,2445,2468],"resource-technique":[2318,2362],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43415","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-fr","tag-pfas","industries-application-analyse-de-pfas","industries-application-environnement","product-library-colonnes-lc","product-library-produits-pour-la-chromatographie-en-phase-liquide","product-library-tubes-raccords-lc","resource-technique-chromatographie-en-phase-liquide","resource-technique-ms-ms-fr"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"post_tag":[{"value":2782,"label":"PFAS"}],"industries-application":[{"value":2212,"label":"Analyse 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