{"id":43426,"date":"2021-01-13T00:00:00","date_gmt":"2021-01-13T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/analyse-lc-msms-de-lacrylamide-dans-leau-potable-avec-injection-de-larges-volumes\/"},"modified":"2026-01-28T19:15:51","modified_gmt":"2026-01-28T19:15:51","slug":"analyse-lc-msms-de-lacrylamide-dans-leau-potable-avec-injection-de-larges-volumes","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-fr\/evar3184-fr\/analyse-lc-msms-de-lacrylamide-dans-leau-potable-avec-injection-de-larges-volumes","title":{"rendered":"Analyse LC-MS\/MS de l\u2019Acrylamide dans l\u2019eau potable avec injection de larges volumes"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Atteint les niveaux de sensibilit\u00e9 requis sans pr\u00e9-concentration chronophage de l\u2019\u00e9chantillon.<\/li>\n\n\n\n<li>Combinaison entre r\u00e9tention ad\u00e9quate et cycle d\u2019analyse court avec la colonne Allure Acrylamide 3.0 x 150 mm.<\/li>\n\n\n\n<li>Volume d\u2019injection de 100 \u00b5l pour augmenter le signal et am\u00e9liorer la sensibilit\u00e9 tout en \u00e9vitant les interf\u00e9rents provenant de la matrice.<\/li>\n<\/ul>\n\n\n\n<p>L\u2019acrylamide a de nombreuses applications, que ce soit en tant que r\u00e9actif dans la synth\u00e8se de compos\u00e9s chimiques organiques ou en tant que monom\u00e8re dans la production de polym\u00e8res de polyacrylamide. Cependant, il est \u00e9galement associ\u00e9 \u00e0 des probl\u00e8mes importants de sant\u00e9 publique\u202f: il peut endommager le syst\u00e8me nerveux humain, de nombreux organismes l\u2019ont donc class\u00e9 comme canc\u00e9rog\u00e8ne probable pour l\u2019homme. Toute personne peut \u00eatre expos\u00e9e \u00e0 l\u2019acrylamide \u00e0 travers la consommation de nourriture ayant \u00e9t\u00e9 cuite \u00e0 l\u2019aide de techniques \u00e0 haute temp\u00e9rature, telle que la friture ou le r\u00f4tissage, mais une exposition beaucoup plus large, \u00e0 l\u2019\u00e9chelle de la population, peut r\u00e9sulter de la contamination des r\u00e9serves d\u2019eau. L\u2019analyse de l\u2019acrylamide dans l\u2019eau potable est en cons\u00e9quence une priorit\u00e9 croissante pour les laboratoires du monde entier.<\/p>\n\n\n\n<p>Les polyacrylamides sont utilis\u00e9s comme agents de floculation dans le traitement des d\u00e9chets et de l\u2019eau potable. L\u2019eau entrante peut avoir une concentration \u00e9lev\u00e9e de particules en suspension, et les usines de traitement utilisent des produits chimiques pour d\u00e9stabiliser ces suspensions (coagulation) puis en provoquer l\u2019agr\u00e9gation une fois d\u00e9stabilis\u00e9es, formant des particules de plus en plus grosses qui se d\u00e9posent alors plus facilement hors de l\u2019eau (floculation). Les agents de floculation \u00e0 base de polyacrylamides sont particuli\u00e8rement efficaces, mais ils peuvent lib\u00e9rer de l\u2019acrylamide monom\u00e9rique, donc pour \u00eatre utilis\u00e9s, ils ne doivent lib\u00e9rer que de tr\u00e8s faibles niveaux d\u2019acrylamide. Traditionnellement, la teneur en acrylamide dans l\u2019eau \u00e9tait calcul\u00e9e \u00e0 partir du pourcentage d\u2019acrylamide monom\u00e9rique r\u00e9siduelle dans le(s) polyacrylamide(s) utilis\u00e9(s) pour le proc\u00e9d\u00e9 de floculation. Mais les techniques de chromatographie liquide ayant consid\u00e9rablement \u00e9volu\u00e9, elles permettent d\u00e9sormais d\u2019analyser l\u2019acrylamide directement dans l\u2019eau potable. De nombreux laboratoires environnementaux utilisent maintenant ces analyses \u00ab\u202fdirectes\u202f\u00bb et on remarque un int\u00e9r\u00eat grandissant pour les techniques tr\u00e8s sensibles, telles que l\u2019injection de larges volumes.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Atteindre les niveaux de sensibilit\u00e9 requis<\/h2>\n\n\n\n<p>L\u2019analyse de l\u2019acrylamide dans l\u2019eau potable est g\u00e9n\u00e9ralement faite en LC-MS\/MS, mais il est difficile de retenir l\u2019acrylamide et de la s\u00e9parer des compos\u00e9s interf\u00e9rents provenant de la matrice avec la plupart des colonnes LC de phase inverse. Cette analyse de l\u2019acrylamide dans l\u2019eau potable est d\u2019autant plus difficile que les limites de d\u00e9tection et de quantification dans cette matrice sont tr\u00e8s faibles, et les niveaux requis de l\u2019ordre du de la ppt peuvent \u00eatre difficiles \u00e0 atteindre, en particulier sur des syst\u00e8mes LC-MS\/MS d\u2019ancienne g\u00e9n\u00e9ration.<\/p>\n\n\n\n<p>La premi\u00e8re \u00e9tape pour atteindre les niveaux de sensibilit\u00e9 requis consiste \u00e0 utiliser une colonne dont la phase stationnaire et le type de particules offrent la r\u00e9tention ad\u00e9quate pour l\u2019acrylamide ainsi qu\u2019une s\u00e9paration efficace des interf\u00e9rents provenant de la matrice tout en gardant des temps d\u2019analyse les plus courts possibles. C\u2019est justement ce que vous offrent les colonnes Restek Allure Acrylamide\u202f! Elles ont \u00e9t\u00e9 d\u00e9velopp\u00e9es sp\u00e9cifiquement pour cette analyse et contiennent des particules avec une phase stationnaire greff\u00e9e ayant une affinit\u00e9 particuli\u00e8re avec les mol\u00e9cules polaires mais \u00e9galement des pores de petites tailles qui vont augmenter la r\u00e9tention des petites mol\u00e9cules polaires, telle que l\u2019acrylamide.<\/p>\n\n\n\n<p>La deuxi\u00e8me \u00e9tape pour atteindre les niveaux de sensibilit\u00e9 requis consiste \u00e0 d\u00e9terminer laquelle des deux strat\u00e9gies les plus couramment utilis\u00e9es convient le mieux \u00e0 votre laboratoire\u202f: (1) pr\u00e9-concentration hors ligne avec extraction en phase solide (SPE), ou (2) injection de larges volumes. Pour les laboratoires utilisant la pr\u00e9-concentration SPE, une colonne Allure Acrylamide en 2.1 x 50 mm est un bon choix. De nombreux laboratoires utilisent plut\u00f4t l\u2019injection de larges volumes, car cela leur permet de gagner du temps et de l\u2019argent tout en r\u00e9duisant potentiellement les risques d\u2019erreurs\u202f; mais d\u2019autres crit\u00e8res rentrent alors en compte, comme les effets matrices par exemple. Pour cette strat\u00e9gie, une colonne Allure Acrylamide en 3.0 x 150 mm sera un choix ad\u00e9quat car elle fournit la capacit\u00e9 n\u00e9cessaire et utilise un d\u00e9bit optimal suffisamment faible pour \u00e9viter une perte de sensibilit\u00e9 en MS. Si vous utilisez une colonne aux dimensions trop grandes, les gains en capacit\u00e9 seraient perdus soit \u00e0 cause d\u2019une perte de sensibilit\u00e9 en MS (si vous utilisez le d\u00e9bit optimal de la colonne, car il est sup\u00e9rieur au d\u00e9bit MS optimal), soit par une perte d\u2019efficacit\u00e9 et de temps (si vous utilisez le d\u00e9bit MS optimal, car il est plus faible\u202fque le d\u00e9bit optimal de la colonne). Il faut enfin \u00e9galement garder \u00e0 l\u2019esprit que la sensibilit\u00e9 inh\u00e9rente \u00e0 l\u2019instrument utilis\u00e9 peut aussi dicter la quantit\u00e9 d\u2019\u00e9chantillon \u00e0 injecter.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Minimiser les effets matrice<\/h2>\n\n\n\n<p>Les compos\u00e9s interf\u00e9rents provenant de la matrice peuvent r\u00e9duire la sensibilit\u00e9 et la pr\u00e9cision de la m\u00e9thode, en particulier lorsque l\u2019on travaille \u00e0 de faibles concentrations, et leurs effets peuvent \u00eatre exacerb\u00e9s lorsque l\u2019on utilise des volumes d\u2019injection (tr\u00e8s) \u00e9lev\u00e9s. Pour d\u00e9montrer ces effets, l\u2019analyse LC-MS\/MS de l\u2019acrylamide dans l\u2019eau potable illustr\u00e9e en figure 1 compare l\u2019influence de la matrice sur trois volumes d\u2019injection diff\u00e9rents. Les effets matrices peuvent varier selon l\u2019\u00e9chantillon, mais nous observons ici que la r\u00e9gion de suppression ionique est de plus en plus grande au fur et \u00e0 mesure que les volumes d\u2019injection augmentent. Les compos\u00e9s de la matrice \u00e9luant t\u00f4t sont g\u00e9n\u00e9ralement envoy\u00e9s \u00e0 la poubelle pour ne pas contaminer la MS, mais nous les montrons ici pour illustrer ces effets matrices et souligner l\u2019importance d\u2019une r\u00e9tention ad\u00e9quate des analytes. Avec un \u00ab\u202fpetit\u202f\u00bb volume d\u2019injection (50 \u00b5l dans ce cas), la zone de suppression ionique se termine bien avant l\u2019\u00e9lution de l\u2019acrylamide, mais le volume est trop faible pour atteindre les niveaux de sensibilit\u00e9 souhait\u00e9s. En passant sur un volume d\u2019injection de 100\u2009\u00b5l, on augmente le signal de l\u2019acrylamide, atteignant la sensibilit\u00e9 requise tout en maintenant une forme de pic acceptable. Malheureusement, il y a des limites \u00e0 la quantit\u00e9 d\u2019\u00e9chantillon qui peut \u00eatre inject\u00e9e tout en gardant de bonnes performances chromatographiques. Par exemple, lorsqu\u2019on injecte 200 \u00b5l, la zone de suppression devient nettement plus importante et commence \u00e0 empi\u00e9ter sur le pic d\u2019acrylamide, r\u00e9sultant en une augmentation du signal plus faible que celle attendue en doublant le volume d\u2019injection. L\u00e0 o\u00f9 une colonne aux dimensions suffisantes pour s\u2019accommoder d\u2019un volume d\u2019injection de 200 \u00b5l aura des soucis li\u00e9s au d\u00e9bit de travail (comme discut\u00e9 auparavant) et aux effets matrice, des injections de 100\u00b5l sur une colonne Allure Acrylamide en 3.0 x 150 mm minimiseront les effets matrice et permettront de r\u00e9pondre aux exigences de sensibilit\u00e9.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1 :<\/strong>\u00a0Le volume d\u2019injection doit \u00eatre suffisamment important pour atteindre les niveaux de sensibilit\u00e9 requis, mais pas trop \u00e9lev\u00e9 pour que la zone de suppression ne vienne pas empi\u00e9ter sur le pic d\u2019acrylamide et ne fasse diminuer le signal.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0565.png\" alt=\"Injection Volume Test of Acrylamide in Tap Water using the Allure Acrylamide LC Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0565<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acrylamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-06-1\/Acrylamide\" target=\"_blank\" rel=\"noopener\">Acrylamide<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">55.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Allure Acrylamide  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916756E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0565\" rel=\"noopener\">cat.# 916756E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 3 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>60 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Allure Acrylamide guard cartridge 10 mm, 2.1 mm ID, 5 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916750212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0565\" rel=\"noopener\">cat.# 916750212<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Acrylamide (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/30494?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0565\" rel=\"noopener\">cat.# 30494<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Tap water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>variable \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.001% Formic acid in water <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>100<\/td><\/tr><tr><td>5.5<\/td><td>0.8<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Tap water fortified with an acrylamide standard to a final concentration of 100 ng\/L (ppt), which was then directly injected at 50, 100, and 200 \u00b5L injection volumes.  <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Note the encroaching suppression zone resulting from ions present in the tap water.  In 50 and 100 \u00b5L injection volumes, acrylamide is well separated from this matrix effect.  At 200 \u00b5L injection volume, the matrix interference affects the acrylamide peak shape and signal.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0565.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<h2 class=\"wp-block-heading\">Performances de la m\u00e9thode<\/h2>\n\n\n\n<p>D\u2019apr\u00e8s nos recherches et essais, des injections de 100 \u00b5l sur une colonne Allure Acrylamide 3.0 x 150 mm dans les conditions indiqu\u00e9es dans la figure 2 se sont r\u00e9v\u00e9l\u00e9es \u00eatre le choix optimal pour l\u2019analyse de l\u2019acrylamide dans l\u2019eau potable par injection directe de larges volumes. Cette m\u00e9thode \u00e9quilibre parfaitement les besoins simultan\u00e9s de capacit\u00e9, de sensibilit\u00e9 et de\u202ftemps d\u2019analyse. Sur un instrument LC-MS\/MS de sensibilit\u00e9 moyenne, nous avons pu obtenir une gamme d\u2019\u00e9talonnage de 30 \u00e0 1000 ppt, atteignant ais\u00e9ment une limite de quantification (LOQ) de 100 ppt et une limite de d\u00e9tection (LOD) de 30 ppt (figures 2 et 3).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2 :<\/strong>\u00a0Une limite de quantification (LOQ) de 100 ppt pour l\u2019analyse de l\u2019acrylamide dans l\u2019eau peut \u00eatre ais\u00e9ment atteinte \u00e0 l\u2019aide de cette m\u00e9thode d\u2019injection de larges volumes.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0566.png\" alt=\"Comparison of 100 ppt Acrylamide in Tap Water to Unfortified Tap Water on Allure Acrylamide\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0566<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acrylamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-06-1\/Acrylamide\" target=\"_blank\" rel=\"noopener\">Acrylamide<\/a><\/td><td class=\"oth\">4.586<\/td><td class=\"oth\">100<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">55.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Allure Acrylamide  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916756E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0566\" rel=\"noopener\">cat.# 916756E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 3 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>60 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Allure Acrylamide guard cartridge 10 mm, 2.1 mm ID, 5 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916750212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0566\" rel=\"noopener\">cat.# 916750212<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Acrylamide (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/30494?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0566\" rel=\"noopener\">cat.# 30494<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Tap water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>100 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.001% Formic acid in water <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>100<\/td><\/tr><tr><td>5.5<\/td><td>0.8<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Tap water samples (1) fortified with 100 ppt acrylamide and (2) neat.  <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Direct injection with 100 \u00b5L injection volume.  <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0566.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3 :<\/strong>\u00a0Courbe d\u2019\u00e9talonnage (30 \u2013 1000 ppt) pour l\u2019acrylamide dans l\u2019eau potable (encart : \u00e9chantillon LOD 30 ppt).<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"657\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-EVAR3184A-03-1024x657.jpg\" alt=\"\" class=\"wp-image-19530\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3184A-03-1024x657.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3184A-03-300x193.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3184A-03-768x493.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3184A-03-1536x986.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3184A-03.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<p>En conclusion, alors que la demande pour l\u2019analyse en injection directe de l\u2019acrylamide dans l\u2019eau potable \u00e0 l\u2019\u00e9tat de traces augmente, l\u2019injection de larges volumes est un moyen rapide et rentable pour r\u00e9pondre aux exigences de sensibilit\u00e9 en comparaison \u00e0 une analyse avec pr\u00e9-concentration SPE. Comme nous l\u2019avons vu dans cet article , utiliser un volume d\u2019injection de 100 \u00b5l sur une colonne Allure Acrylamide 3.0 x 150 mm est un moyen efficace pour employer cette approche.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Cette analyse LC-MS\/MS d&#8217;injection de larges volumes permet d&#8217;atteindre les limites de d\u00e9tection et de quantification de l&#8217;ordre du ppt requises pour l&#8217;analyse de l&#8217;acrylamide dans l&#8217;eau 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