{"id":43479,"date":"2020-10-27T00:00:00","date_gmt":"2020-10-27T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/method-505-analysis-of-organohalide-pesticides-and-commercial-pcbs-in-water-by-microextraction-and-gc\/"},"modified":"2026-01-02T18:25:38","modified_gmt":"2026-01-02T18:25:38","slug":"method-505-analysis-of-organohalide-pesticides-and-commercial-pcbs-in-water-by-microextraction-and-gc","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles\/evar4504\/method-505-analysis-of-organohalide-pesticides-and-commercial-pcbs-in-water-by-microextraction-and-gc","title":{"rendered":"Method 505: Analysis of Organohalide Pesticides and Commercial PCBs in Water by Microextraction and GC"},"content":{"rendered":"\n

With the advent of modern agriculture and its vast selection of chemical pest control measures, the farming community has made significant increases in productivity and efficiency. Crop yield per acre is extremely high, due in part to the role of pesticides and herbicides in mitigating the devastating effects of many plant and insect pests. However, the use of these chemicals can have drawbacks, including surface and ground water contamination. EPA Method 505 is used for organohalide pesticide analysis in drinking and ground water. The Rtx-CLPesticides and Rtx-CLPesticides2 column set works well for the analysis of organohalide compounds because the columns are highly selective for analytes that contain electronegative substituents. The optimized parallel dual-column method shown here provides fast analysis times, which improves sample throughput.<\/p>\n\n\n\n

Vendor<\/th>Column Pair<\/th>Analysis Time (min)<\/th>Coelutions<\/th>Restek Advantage<\/th><\/tr>
Restek<\/td>Rtx-CLPesticides<\/a> \/
Rtx-CLPesticides2<\/a><\/td>		18 \/ 18.5<\/td>1 \/ 1<\/td>All compounds resolved on at least one column.<\/td><\/tr>
Competitor A<\/th>column 1 \/ column 2<\/td>14 \/ 14<\/td>0 \/ 1<\/td><\/tr>
Competitor B<\/th>column 1 \/ column 2<\/td>35 \/ 36<\/td>1 \/ 2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Comparison based on published competitor data. All columns tested were 0.32 mm ID.<\/p>\n\n\n

Organohalide pesticide analysis (Method 505) on Rtx-CLPesticides and Rtx-CLPesticides2 columns.<\/strong><\/div>
\n
\"Pesticides<\/div>

GC_EV01004<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th><\/tr><\/thead>\n
1.<\/td>Hexachlorocyclopentadiene<\/a><\/td><\/tr>\n
2.<\/td>Hexachlorobenzene<\/a><\/td><\/tr>\n
3.<\/td>Simazine<\/a><\/td><\/tr>\n
4.<\/td>Atrazine<\/a><\/td><\/tr>\n
5.<\/td>\u03b3-BHC<\/a><\/td><\/tr>\n
6.<\/td>Heptachlor<\/a><\/td><\/tr>\n
7.<\/td>Alachlor<\/a><\/td><\/tr>\n
8.<\/td>Aldrin<\/a><\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th><\/tr><\/thead>
9.<\/td>Heptachlor epoxide<\/a><\/td><\/tr>\n
10.<\/td>trans-Chlordane<\/a><\/td><\/tr>\n
11.<\/td>trans-Nonachlor<\/a><\/td><\/tr>\n
12.<\/td>cis-Chlordane<\/a><\/td><\/tr>\n
13.<\/td>Dieldrin<\/a><\/td><\/tr>\n
14.<\/td>Endrin<\/a><\/td><\/tr>\n
15.<\/td>cis-Nonachlor<\/a><\/td><\/tr>\n
16.<\/td>Methoxychlor<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div>

Conditions<\/h4>
Columns<\/th>Rtx-CLPesticides 30 m, 0.32 mm ID, 0.32 \u00b5m (cat.# 11141<\/a>)
<\/td>and Rtx-CLPesticides2 30 m, 0.32 mm ID, 0.25 \u00b5m (cat.# 11324<\/a>)
<\/td>using Rxi guard column 5 m, 0.32 mm ID (cat.# 10039<\/a>)
<\/td>with deactivated universal \u201cY\u201d Press-Tight connector (cat.# 20405-261<\/a>)
<\/td>
Standard\/Sample<\/th><\/td><\/tr>
<\/td>200 ng\/mL 505 organohalide pesticide mix (cat.# 32024<\/a>)<\/td><\/tr>
<\/td>4.2 \u00b5g\/mL simazine (cat.# 32236<\/a>)<\/td><\/tr>
<\/td>4.2 \u00b5g\/mL atrazine (cat.# 32208<\/a>)<\/td><\/tr>\n
Diluent:<\/th>Methanol<\/td><\/tr>
Injection<\/th><\/td><\/tr>
Inj. Vol.:<\/th>2 \u00b5L splitless (hold 0.75 min)<\/td><\/tr>
Liner:<\/th>Cyclo double taper (4 mm) (cat.# 20896<\/a>)<\/td><\/tr>
Inj. Temp.:<\/th>250 \u00b0C<\/td><\/tr>
Oven<\/td><\/tr>
Oven Temp.:<\/th>90 °C (hold 1 min) to 310 °C at 10 °C\/min (hold 5 min)<\/td><\/tr>
Carrier Gas<\/th>He, constant flow<\/td><\/tr>
Linear Velocity:<\/th>40 cm\/sec<\/td><\/tr><\/table>
Detector<\/th>Micro-ECD @ 325 \u00b0C<\/td><\/tr>
Instrument<\/th>Agilent\/HP6890 GC<\/td><\/tr>
Notes<\/th>This chromatogram was obtained using an Agilent micro-ECD. To obtain comparable results, you will need to employ a micro-ECD in addition to confirmational dual columns connected to a 5-meter guard column using a \u201cY\u201d Press-Tight connector.<\/td><\/tr><\/table><\/div><\/div><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n

<\/p>\n\n\n\n

<\/div>\n\n\n
\n

Products Mentioned<\/h3>\n
\n
\n
\n
\n Catalog No. 11196 <\/a> <\/div>\n
\n
Kit colonne capillaire Rtx-CLPesticides, DI 0.32\u00a0mm, 0.32 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 11120 <\/a> <\/div>\n
\n
Colonne capillaire Rtx-CLPesticides, L 15\u00a0m, DI 0.25 mm, 0.25 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 11321 <\/a> <\/div>\n
\n
Colonne capillaire Rtx-CLPesticides2, L 15 m, DI 0.32 mm, 0.25 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

Rtx-CLPesticides and Rtx-CLPesticides2 columns are ideal for multiple environmental ECD methods. Here we demonstrate the effectiveness of this column pair for organohalide pesticides by EPA Method 505.<\/p>\n","protected":false},"author":11,"featured_media":6751,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2163,2161,2668],"post-badge":[],"resource-type":[],"product-library":[2372,2384,2399,2374],"resource-technique":[2294],"ppma_author":[414],"class_list":["post-43479","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","industries-application-drinking-water-wastewater-testing","industries-application-environmental","industries-application-semivolatiles","product-library-gas-chromatography-products","product-library-gc-columns","product-library-liquid-reference-standards","product-library-reference-standard-products","resource-technique-gas-chromatography-gc"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2163,"label":"Drinking Water & Wastewater Testing"},{"value":2161,"label":"Environmental"},{"value":2668,"label":"Semivolatiles"}],"product-library":[{"value":2372,"label":"Gas Chromatography Products"},{"value":2384,"label":"GC Columns"},{"value":2399,"label":"Liquid Reference Standards"},{"value":2374,"label":"Reference Standard Products"}],"resource-technique":[{"value":2294,"label":"Gas Chromatography (GC)"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-EVSS1013-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/fr\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":7,"name":"Articles","slug":"articles","term_group":0,"term_taxonomy_id":7,"taxonomy":"category","description":"","parent":0,"count":465,"filter":"raw","cat_ID":7,"category_count":465,"category_description":"","cat_name":"Articles","category_nicename":"articles","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43479","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=43479"}],"version-history":[{"count":7,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43479\/revisions"}],"predecessor-version":[{"id":79168,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43479\/revisions\/79168"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media\/6751"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=43479"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=43479"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=43479"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=43479"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=43479"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=43479"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=43479"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=43479"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=43479"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}