{"id":43522,"date":"2020-10-20T00:00:00","date_gmt":"2020-10-20T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/analyse-du-paraquat-et-du-diquat-par-lc-msms-sans-reactif-dappariement-dions\/"},"modified":"2026-02-18T18:21:55","modified_gmt":"2026-02-18T18:21:55","slug":"analyse-du-paraquat-et-du-diquat-par-lc-msms-sans-reactif-dappariement-dions","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-fr\/evss2791-fr\/analyse-du-paraquat-et-du-diquat-par-lc-msms-sans-reactif-dappariement-dions","title":{"rendered":"Analyse du Paraquat et du Diquat par LC-MS\/MS sans r\u00e9actif d\u2019appariement d\u2019ions"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Bonne r\u00e9tention et bonne forme de pic sans contamination du syst\u00e8me par des r\u00e9actifs d&#8217;appariement d&#8217;ions.<\/li>\n\n\n\n<li>S\u00e9paration et r\u00e9ponse r\u00e9guli\u00e8res avec les phases mobiles compatibles avec la MS.<\/li>\n\n\n\n<li>Rapide, cette m\u00e9thode de 7 minutes avec un gradient permet un d\u00e9bit de traitement \u00e9lev\u00e9.<\/li>\n<\/ul>\n\n\n\n<p>Le Paraquat est un herbicide de contact non s\u00e9lectif relativement bon march\u00e9. Gr\u00e2ce \u00e0 ces caract\u00e9ristiques il est tr\u00e8s utilis\u00e9 dans les cultures agricoles, dans une grande partie des pays en d\u00e9veloppement. Si les pr\u00e9cautions ad\u00e9quates sont respect\u00e9es, l\u2019application du Paraquat repr\u00e9sente peu de danger, mais le taux de mortalit\u00e9 li\u00e9 aux ingestions accidentelles ou d\u00e9lib\u00e9r\u00e9es est extr\u00eamement \u00e9lev\u00e9. C\u2019est en grande partie pour cette raison que le Paraquat fait l\u2019objet de restrictions dans de nombreux pays. Le Diquat est un herbicide de la m\u00eame famille qui est souvent utilis\u00e9 en m\u00eame temps que le Paraquat. Il est sous surveillance du fait de ses effets toxiques possibles sur le syst\u00e8me nerveux central. L\u2019analyse par LC-MS\/MS du Paraquat et du Diquat dans les cultures vivri\u00e8res \u00e0 faible teneur en eau, dans l\u2019eau potable et dans les eaux de surface est un outil important pour pr\u00e9venir ou pour limiter l\u2019exposition humaine et environnementale.<\/p>\n\n\n\n<p>Habituellement, des r\u00e9actifs d\u2019appariement d\u2019ions sont utilis\u00e9s pour analyser le Paraquat et le Diquat par LC-MS\/MS, afin d\u2019obtenir une r\u00e9ponse ad\u00e9quate en mati\u00e8re de r\u00e9tention, de r\u00e9solution et de forme des pics. Cependant, les r\u00e9actifs d\u2019appariement d\u2019ions concurrencent les analytes cibles pendant l&#8217;ionisation, ce qui r\u00e9duit habituellement la sensibilit\u00e9. Ces r\u00e9actifs peuvent aussi contaminer le syst\u00e8me LC-MS\/MS, ce qui oblige \u00e0 le d\u00e9monter fr\u00e9quemment pour un nettoyage complet.<\/p>\n\n\n\n<p>Ici, nous avons d\u00e9velopp\u00e9 une analyse du Paraquat et du Diquat par LC-MS\/MS qui n\u2019utilise pas de r\u00e9actif d\u2019appariement d\u2019ions, mais qui offre quand m\u00eame une bonne r\u00e9tention et une bonne forme de pic. La r\u00e9solution des deux analytes cibles \u00e9tait compl\u00e8te et \u00e0 50 ng\/ml (50 ppb), la sensibilit\u00e9 \u00e9tait bien meilleure qu\u2019avec les m\u00e9thodes utilisant l\u2019appariement d\u2019ions. Cette analyse rapide en 7 minutes a fourni des r\u00e9sultats constants en MS, en utilisant une phase mobile avec tampon 50 mMole, conform\u00e9ment aux concentrations de sels limites recommand\u00e9es pour r\u00e9duire au minimum la contamination et la maintenance des syst\u00e8mes LC-MS\/MS.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0512.png\" alt=\"Paraquat and Diquat on Raptor HILIC-Si\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0512<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/6385-62-2\/Diquat\" target=\"_blank\" rel=\"noopener\">Diquat<\/a><\/td><td class=\"oth\">3.25<\/td><td class=\"oth\">183.3<\/td><td class=\"oth\">157.2<\/td><td class=\"oth\">183.3<\/td><td class=\"oth\">130.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Paraquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1910-42-5\/Paraquat\" target=\"_blank\" rel=\"noopener\">Paraquat<\/a><\/td><td class=\"oth\">3.46<\/td><td class=\"oth\">185.3<\/td><td class=\"oth\">170.3<\/td><td class=\"oth\">171.3<\/td><td class=\"oth\">77.2<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0512\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>45 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Paraquat &amp; diquat calibration mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/32437?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0512\" rel=\"noopener\">cat.# 32437<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase B<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 50 mM ammonium formate, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>25:75 Water:acetonitrile, 50 mM ammonium formate, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>4.00<\/td><td>0.6<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>4.01<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>7.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0512.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>L\u2019analyse par LC-MS\/MS du Paraquat et du Diquatest un outil important pour pr\u00e9venir ou pour limiter l\u2019exposition humaine et environnementale. Habituellement, des r\u00e9actifs d\u2019appariement d\u2019ions sont utilis\u00e9s pour analyser le Paraquat et le Diquat par LC-MS\/MS, afin d\u2019obtenir une r\u00e9ponse ad\u00e9quate en mati\u00e8re de r\u00e9tention, de r\u00e9solution et de forme des pics. Cependant, les r\u00e9actifs d\u2019appariement d\u2019ions concurrencent les analytes cibles pendant l&#8217;ionisation, ce qui r\u00e9duit habituellement la sensibilit\u00e9. Ces r\u00e9actifs peuvent aussi contaminer le syst\u00e8me LC-MS\/MS, ce qui oblige \u00e0 le d\u00e9monter fr\u00e9quemment pour un nettoyage complet. L&#8217;analyse LC-MS\/MS pr\u00e9sent\u00e9e ici se r\u00e9v\u00e8le fiable pour les deux analytes cibles dans un gradient rapide de 7 minutes sans l&#8217;utilisation de r\u00e9actifs d&#8217;appariement d&#8217;ions g\u00eanants.<\/p>\n","protected":false},"author":11,"featured_media":6783,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[],"industries-application":[2210,2208,2758],"post-badge":[],"resource-type":[],"product-library":[2463,2446,2471,2445],"resource-technique":[2318,2362,2320],"ppma_author":[414],"class_list":["post-43522","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-fr","industries-application-eau-potable-eaux-usees","industries-application-environnement","industries-application-semivolatiles","product-library-colonnes-lc","product-library-etalons-de-reference","product-library-etalons-en-solution","product-library-produits-pour-la-chromatographie-en-phase-liquide","resource-technique-chromatographie-en-phase-liquide","resource-technique-ms-ms-fr","resource-technique-spectrometrie-de-masse-ms"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"industries-application":[{"value":2210,"label":"Eau 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