{"id":43626,"date":"2021-06-25T14:30:00","date_gmt":"2021-06-25T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/revive-in-line-sample-preparation-ilsp-une-approche-plus-rapide-pour-lanalyse-multi-residus-de-pesticides-dans-les-aliments\/"},"modified":"2026-01-28T21:46:16","modified_gmt":"2026-01-28T21:46:16","slug":"revive-in-line-sample-preparation-ilsp-une-approche-plus-rapide-pour-lanalyse-multi-residus-de-pesticides-dans-les-aliments","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-fr\/fsss3549-fr\/revive-in-line-sample-preparation-ilsp-une-approche-plus-rapide-pour-lanalyse-multi-residus-de-pesticides-dans-les-aliments","title":{"rendered":"Revive In-Line Sample Preparation (ILSP): Une approche plus rapide pour l\u2019analyse multi-r\u00e9sidus de pesticides dans les aliments"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>La purification en ligne et automatis\u00e9e des \u00e9chantillons r\u00e9duit consid\u00e9rablement leur temps de pr\u00e9paration.<\/li>\n\n\n\n<li>Le nettoyage de la cartouche ILSP se fait pendant l\u2019analyse, ce qui permet d\u2019encha\u00eener les \u00e9chantillons tr\u00e8s rapidement.<\/li>\n\n\n\n<li>Alternative simple et rapide aux techniques QuEChERS ou SPE pour l\u2019analyse multi-r\u00e9sidus de pesticides dans les aliments.<\/li>\n<\/ul>\n\n\n\n<p>La pr\u00e9paration d\u2019\u00e9chantillons en ligne ILSP est une technique de pr\u00e9paration d\u2019\u00e9chantillons id\u00e9ale pour les laboratoires de s\u00e9curit\u00e9 alimentaire qui cherchent \u00e0 \u00e9conomiser du temps et de l\u2019argent sur leurs analyses LC-MS\/MS sans sacrifier les performances. Une cartouche Revive ILSP Pesticides s\u00e9pare les analytes d\u2019int\u00e9r\u00eat des interf\u00e9rences matricielles potentielles, comme le font les m\u00e9thodes traditionnelles SPE et QuEChERS, mais elle remplace les proc\u00e9dures manuelles fastidieuses par un processus automatis\u00e9 et&nbsp;<em>en ligne<\/em>&nbsp;de purification puis d\u2019analyse de l\u2019\u00e9chantillon. Gr\u00e2ce \u00e0 l\u2019efficacit\u00e9 de leurs m\u00e9canismes de r\u00e9tention (par exemple des interactions de phase inverse) et de la conception sp\u00e9cifique de leurs particules, les cartouches Revive ILSP Pesticides promettent une purification chromatographique efficace et automatis\u00e9e d\u2019\u00e9chantillons complexes ayant lieu en m\u00eame temps que l\u2019analyse de ces \u00e9chantillons.<\/p>\n\n\n\n<p>Afin de d\u00e9montrer les avantages d\u2019une pr\u00e9paration en ligne, la Figure 1 compare une proc\u00e9dure utilisant la technologie Revive ILSP \u00e0 une proc\u00e9dure QuEChERS typique pour l\u2019analyse de r\u00e9sidus de pesticides dans les \u00e9pinards. L\u2019approche ILSP a permis d\u2019\u00e9conomiser 1,5h avec moins de transfert d\u2019\u00e9chantillons, ce qui a donc entra\u00een\u00e9 une augmentation significative de la productivit\u00e9 en termes de pr\u00e9paration d\u2019\u00e9chantillons. La technologie ILSP diminue \u00e9galement le risque d\u2019erreurs manuelles et fournit des r\u00e9sultats analytiques \u00e9quivalents ou sup\u00e9rieurs aux techniques QuEChERS. Une \u00e9tude exhaustive de cette comparaison a \u00e9t\u00e9 publi\u00e9e par Lupo et al. [1].<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1 :<\/strong>\u00a0La proc\u00e9dure de pr\u00e9paration d\u2019\u00e9chantillons en ligne Revive est 1,5h plus rapide que la proc\u00e9dure traditionnelle QuEChERS pour 14 \u00e9chantillons d\u2019\u00e9pinards.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"790\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-FSSS3549-01-790x1024.jpg\" alt=\"\" class=\"wp-image-20256\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01-790x1024.jpg 790w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01-231x300.jpg 231w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01-768x996.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01-1185x1536.jpg 1185w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01-1579x2048.jpg 1579w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-01.jpg 1800w\" sizes=\"auto, (max-width: 790px) 100vw, 790px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Pr\u00e9paration d\u2019\u00e9chantillons en ligne Revive : comment \u00e7a marche ?<\/h2>\n\n\n\n<p>Comme le montre la Figure 2, la technologie Revive ILSP int\u00e8gre une cartouche de purification de l\u2019\u00e9chantillon directement dans le circuit d\u2019injection LC. Une fois que les analytes ont atteint la colonne analytique, la cartouche de purification ILSP est alors rinc\u00e9e (&#8220;revived&#8221;) par &#8220;backflush&#8221; et remise en condition pour l\u2019analyse suivante pendant que le premier \u00e9chantillon est quant \u00e0 lui analys\u00e9. Ces r\u00e9g\u00e9n\u00e9ration et analyse simultan\u00e9es sont rendues possibles gr\u00e2ce \u00e0 l\u2019utilisation d\u2019une vanne 6 voies et d\u2019une pompe isocratique standard ind\u00e9pendante. Notre\u00a0<a href=\"https:\/\/discover.restek.com\/fsar3550-fr\/developpement-de-methodes-ilsp-in-line-sample-preparation\/\" data-type=\"post\" data-id=\"43595\">article \u00e0 propos du d\u00e9veloppement des m\u00e9thodes ILSP<\/a>\u00a0vous fournira tous les conseils n\u00e9cessaires et d\u00e9taill\u00e9s sur la configuration de l\u2019instrument, le r\u00e9glage des param\u00e8tres de la m\u00e9thode (par exemple la synchronisation de la vanne ou le d\u00e9bit de solvant utilis\u00e9 pour le rin\u00e7age de la cartouche) ou encore comment identifier le solvant de rin\u00e7age le plus appropri\u00e9. Les \u00e9conomies quotidiennes r\u00e9alis\u00e9es sur les co\u00fbts d\u2019exploitation compenseront tr\u00e8s rapidement l\u2019investissement dans une pompe standard et le temps pass\u00e9 \u00e0 configurer la m\u00e9thode, en particulier pour les laboratoires ayant un nombre \u00e9lev\u00e9 d\u2019\u00e9chantillons \u00e0 analyser ou pour ceux ayant des ressources limit\u00e9es au niveau de la pr\u00e9paration des \u00e9chantillons.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2 :<\/strong>\u00a0Principe de fonctionnement de la technologie ILSP<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"897\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-FSSS3549-02-1024x897.jpg\" alt=\"\" class=\"wp-image-20268\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-02-1024x897.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-02-300x263.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-02-768x672.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-02-1536x1345.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-02.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Des r\u00e9sultats r\u00e9p\u00e9tables et reproductifs, des analyses plus rentables et de plus longues dur\u00e9es de vie<\/h2>\n\n\n\n<p>Au-del\u00e0 du gain de temps, une exp\u00e9rience men\u00e9e sur la dur\u00e9e de vie des cartouches avec 300 injections d\u2019extraits d\u2019avocat fournit un excellent exemple de la constance des r\u00e9sultats et des \u00e9conomies substantielles qu\u2019offre la technologie ILSP. L\u2019avocat, un produit riche en mati\u00e8res grasses, a \u00e9t\u00e9 dop\u00e9 avec un m\u00e9lange repr\u00e9sentatif de 61 pesticides ayant des caract\u00e9ristiques chimiques et des temps de r\u00e9tention diff\u00e9rents. Les \u00e9chantillons ont ensuite \u00e9t\u00e9 extraits et analys\u00e9s en suivant la proc\u00e9dure optimis\u00e9e illustr\u00e9e dans la Figure 3. La m\u00e9thode de pr\u00e9paration en ligne des \u00e9chantillons \u00e9tait significativement plus rapide que la proc\u00e9dure QuEChERS comparable, et une seule et unique cartouche Revive ILSP Pesticides a pu fournir une purification efficace et des r\u00e9sultats chromatographiques constants et coh\u00e9rents tout au long de la dur\u00e9e de l\u2019exp\u00e9rience.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3 :<\/strong>\u00a0Un exemple de protocole de pr\u00e9paration d\u2019\u00e9chantillons en ligne ILSP pour des \u00e9chantillons d\u2019avocats riches en mati\u00e8res grasses.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"922\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-FSSS3549-03-1024x922.jpg\" alt=\"\" class=\"wp-image-20280\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-03-1024x922.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-03-300x270.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-03-768x691.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-03-1536x1382.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FSSS3549-03.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<p>Les trois pesticides mis en avant dans la Figure 4 sont repr\u00e9sentatifs des compos\u00e9s ayant une faible r\u00e9tention, une r\u00e9tention moyenne et une forte r\u00e9tention. La stabilit\u00e9 des temps de r\u00e9tention, des formes de pics et des r\u00e9ponses, sur plusieurs centaines d\u2019injections de matrice, d\u00e9montre la robustesse de la purification des cartouches Revive ILSP Pesticides. Sur les 61 pesticides analys\u00e9s sous forme d\u2019une s\u00e9rie de triple injection d\u2019un seul extrait [2], 95% d\u2019entre eux ont satisfait aux crit\u00e8res de performance \u00e9tablis par les directives SANTE\/12682\/2019 des Laboratoires de R\u00e9f\u00e9rence de l\u2019Union Europ\u00e9enne (EURL) \u2013 rendements entre 70 et 120% et %RSD \u2264 20%.<\/p>\n\n\n\n<p>En plus des gains en vitesse et des performances robustes, la technologie ILSP permet \u00e9galement une r\u00e9duction des co\u00fbts assez significative. Par exemple, si nous supposons que le co\u00fbt d\u2019un \u00e9chantillon trait\u00e9 par la technique QuEChERS (sachet de sels d\u2019extraction et tube dSPE) co\u00fbte environ 4$, alors les 300 \u00e9chantillons d\u2019avocat seraient revenus \u00e0 1200 $ rien qu&#8217;en produits QuEChERS. La proc\u00e9dure de la pr\u00e9paration en ligne Revive utilise une simple extraction liquide-solide, sans sels d\u2019extraction ou de dSPE, ce qui \u00e9limine donc le co\u00fbt li\u00e9 \u00e0 ces produits. Une seule et unique cartouche Revive ILSP Pesticides a pu purifier efficacement les 300 \u00e9chantillons d\u2019avocat, tout en maintenant ses performances et sans avoir \u00e0 la changer avant la fin de l\u2019exp\u00e9rience, et ce pour un co\u00fbt quatre fois moindre qu&#8217;en utilisant la technique QuEChERS.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4 :<\/strong>\u00a0Chromatographie r\u00e9p\u00e9table m\u00eame apr\u00e8s 300 injections de matrice d\u2019avocat(s ?) sur une m\u00eame cartouche Revive ILSP.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0584.png\" alt=\"ILSP Lifetime Study with Avocado\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0584<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/g)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Imidacloprid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/138261-41-3\/Imidacloprid\" target=\"_blank\" rel=\"noopener\">Imidacloprid<\/a><\/td><td class=\"oth\">2.678<\/td><td class=\"oth\">50<\/td><td class=\"oth\">256.1<\/td><td class=\"oth\">175.0<\/td><td class=\"oth\">209.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fenhexamid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/126833-17-8\/Fenhexamid\" target=\"_blank\" rel=\"noopener\">Fenhexamid<\/a><\/td><td class=\"oth\">4.557<\/td><td class=\"oth\">50<\/td><td class=\"oth\">302.1<\/td><td class=\"oth\">97.1<\/td><td class=\"oth\">55.05<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Eprinomectin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/123997-26-2\/Eprinomectin\" target=\"_blank\" rel=\"noopener\">Eprinomectin<\/a><\/td><td class=\"oth\">6.396<\/td><td class=\"oth\">50<\/td><td class=\"oth\">914.6<\/td><td class=\"oth\">186.1<\/td><td class=\"oth\">154.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0584\" rel=\"noopener\">cat.# 9314A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0584\" rel=\"noopener\">cat.# 9314A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile, 0.1% acetic acid<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>3 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.2% formic acid, 2 mM ammonium formate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.2% formic acid, 2 mM ammonium formate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">C:<\/th><td>Methanol, 0.2% formic acid, 10 mM ammonium formate <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>2<\/td><td>0.4<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>4<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>6<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>8<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>8.01<\/td><td>0.4<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>10<\/td><td>0.4<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td><i>Sample Fortification and Extraction<\/i><br \/>Avocado was peeled and homogenized, and 5 g of sample was weighed into a 50 mL polypropylene tube. 10 mL of acetonitrile containing 0.1% acetic acid was added to the sample and vortexed. Samples were shaken on a shaker table for 10 minutes and then centrifuged at 4200 rpm for 10 minutes. An aliquot was transferred to a vial and fortified with analytes for a final concentration of 50 ng\/g. The sample was vortexed, and an aliquot transferred to a 0.2 \u03bcm PTFE Thomson filter vial cat.# 25893 and filtered prior to injection. <br \/><br \/><i>In-Line Sample Preparation (ILSP)<\/i><br \/>The UHPLC system was equipped with an auxiliary pump; 6-port, high-pressure switching valve; and dual-directional 5 x 2.1 mm Revive ILSP Pesticides cartridge and holder (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/27882?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0584\" rel=\"noopener\">cat.# 27882<\/a>). At 5.5 min, after the target compounds had all eluted from the ILSP cartridge and were undergoing analysis, valve switching was used as described below to flush the ILSP cartridge and wash trapped matrix components to waste. At 7.0 min, the original valve configuration, where the ILSP cartridge is in-line with the analytical column, was restored and the system was brought back to equilibrium prior to the next injection.<br \/>\u2022 0 min; valve position 0<br \/>\u2022 0 min; C flow= 0 mL\/min<br \/>\u2022 5.49 min; C flow= 0 mL\/min<br \/>\u2022 5.5 min; valve position 1<br \/>\u2022 5.5 min; C flow= 1 mL\/min<br \/>\u2022 6.9 min; C flow=1 mL\/min<br \/>\u2022 7 min; C flow= 0 mL\/min<br \/>\u2022 7 min; valve position 0<br \/><br \/><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Thomson SINGLE StEP standard filter vials cat.# 25893 were used to produce this chromatogram, but have since been discontinued. For assistance choosing a replacement for this application, contact Restek Technical Service or your local Restek representative.<br \/><\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0584.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Revive ILSP : Analyse multi-r\u00e9sidus de pesticides dans diff\u00e9rents aliments<\/h2>\n\n\n\n<p>L\u2019analyse de pesticides dans les denr\u00e9es alimentaires est particuli\u00e8rement complexe de par la grande vari\u00e9t\u00e9 des structures chimiques des analytes mais aussi de par la grande diversit\u00e9 des types de matrices (riches en graisses, riches en sucres, riches en pigments, \u00e0 faible teneur en eau, etc.). Pour tenter de r\u00e9soudre ces probl\u00e8mes, de nombreux produits QuEChERS ont \u00e9t\u00e9 d\u00e9velopp\u00e9s et formul\u00e9s pour assurer une extraction et une purification efficaces dans ces diff\u00e9rentes situations. Ici, une seule et unique cartouche Revive ILSP Pesticides peut fournir d\u2019excellents r\u00e9sultats sur la m\u00eame large gamme d\u2019analytes et de matrices.&nbsp;<\/p>\n\n\n\n<p>Pour d\u00e9montrer cette large gamme d\u2019application de la technologie ILSP, une \u00e9tude de rendement a \u00e9t\u00e9 men\u00e9e sur six aliments couvrant 5 des groupes d\u2019aliments d\u00e9crits dans les directives SANTE\/12682\/2019 (Tableau I). Ces aliments ont \u00e9t\u00e9 dop\u00e9s avec 61 pesticides repr\u00e9sentatifs des diff\u00e9rentes chimies des compos\u00e9s et \u00e9luant \u00e0 diff\u00e9rents temps de r\u00e9tention (pr\u00e9coces, moyens, tardifs) sur un chromatogramme typique d\u2019un screening multi-r\u00e9sidus de pesticides (Tableau II).<\/p>\n\n\n\n<p><strong>Tableau I :<\/strong>&nbsp;<strong>Aliments utilis\u00e9s pour d\u00e9velopper les m\u00e9thodes Revive ILSP<\/strong>.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Groupe de produits alimentaires<\/strong><\/td><td><strong>Echantillon<\/strong><\/td><\/tr><tr><td>A teneurs \u00e9lev\u00e9es en eau et en pigments<\/td><td>Epinards<\/td><\/tr><tr><td>A teneurs \u00e9lev\u00e9es en acide et en eau<\/td><td>Orange enti\u00e8re<\/td><\/tr><tr><td>A teneur \u00e9lev\u00e9e en lipides et tr\u00e8s faible teneur en eau<\/td><td>Plat \u00e0 base de soja<\/td><\/tr><tr><td>A teneur \u00e9lev\u00e9e en lipides et teneur en eau interm\u00e9diaire<\/td><td>Avocat<\/td><\/tr><tr><td rowspan=\"2\">&#8220;Produits difficiles ou uniques&#8221;<\/td><td>Th\u00e9 \u00e0 l&#8217;hibiscus<\/td><\/tr><tr><td>Th\u00e9 noir<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>Tableau II :<\/strong>&nbsp;<strong>Les \u00e9tudes de rendements sur les cartouches Revive ILSP ont \u00e9t\u00e9 men\u00e9es en utilisant 61 pesticides qui diff\u00e9raient \u00e0 la fois en termes de caract\u00e9ristiques chimiques&nbsp; et en termes de temps de r\u00e9tention (pr\u00e9coces, moyens, tardifs) dans les m\u00e9thodes typiques de screening&nbsp;multi-r\u00e9sidus de pesticides.<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td colspan=\"3\"><strong>Liste des pesticides<\/strong><\/td><\/tr><tr><td>Cyromazine<\/td><td>Flutolanil<\/td><td>Benzoximate<\/td><\/tr><tr><td>Dinotefuran<\/td><td>Mepronil<\/td><td>Trifloxystrobin<\/td><\/tr><tr><td>Nitenpyram<\/td><td>Myclobutanil<\/td><td>Metaflumizone<\/td><\/tr><tr><td>Imidacloprid<\/td><td>Methoxyfenozide<\/td><td>Fluazinam<\/td><\/tr><tr><td>Acetamiprid<\/td><td>Triadimefon<\/td><td>Tebufenpyrad<\/td><\/tr><tr><td>Oxadixyl<\/td><td>Mepanipyrim<\/td><td>Pyriproxyfen<\/td><\/tr><tr><td>Carbetamide<\/td><td>Fluoxastrobin<\/td><td>Piperonyl Butoxide<\/td><\/tr><tr><td>Pyracarbolid<\/td><td>Fenhexamid<\/td><td>Quinoxyfen<\/td><\/tr><tr><td>Secbumeton<\/td><td>Butafenacil<\/td><td>Amitraz<\/td><\/tr><tr><td>Prometon<\/td><td>Cyprodinil<\/td><td>Fenpyroximate<\/td><\/tr><tr><td>Terbumeton<\/td><td>Picoxystrobin<\/td><td>Eprinomectin<\/td><\/tr><tr><td>Ametryn<\/td><td>Rotenone<\/td><td>Abamectin B1a<\/td><\/tr><tr><td>Metalaxyl<\/td><td>Tebufenozide<\/td><td>Fenazaquin<\/td><\/tr><tr><td>Chlorantraniliprole<\/td><td>Dimoxystrobin<\/td><td>Doramectin<\/td><\/tr><tr><td>Pyrimethanil<\/td><td>Carfentrazone-ethyl +NH4<\/td><td>Ivermectin<\/td><\/tr><tr><td>Spiroxamine<\/td><td>Kresoxym-methyl<\/td><td>Moxidectin<\/td><\/tr><tr><td>Azoxystrobin<\/td><td>Zoxamide<\/td><td>Imazalil<\/td><\/tr><tr><td>Halofenozide<\/td><td>Famoxadone<\/td><td>Pymetrozine<\/td><\/tr><tr><td>Furalaxyl<\/td><td>Benalaxyl<\/td><td>Fludioxinol<\/td><\/tr><tr><td>Boscalid<\/td><td>Clofentezine<\/td><td>&nbsp;<\/td><\/tr><tr><td>Mandipropamid<\/td><td>Prochloraz<\/td><td>&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Extraction ILSP<\/h3>\n\n\n\n<p>Pour la partie extraction, une homog\u00e9n\u00e9isation de l\u2019\u00e9chantillon suivie d\u2019une extraction liquide-solide ont bien fonctionn\u00e9 pour les matrices alimentaires \u00e9tudi\u00e9es. Les param\u00e8tres d\u2019extraction (solvant, volume, temps d\u2019agitation, filtration, etc.) ont \u00e9t\u00e9 optimis\u00e9s pour chaque matrice, mais la proc\u00e9dure globale reste simple, rapide et efficace&nbsp;: homog\u00e9n\u00e9isation, ajout d\u2019un solvant, agitation et filtration de l\u2019\u00e9chantillon si n\u00e9cessaire. Et comme il n\u2019y a pas d\u2019\u00e9tape de purification par SPE dispersive comme c\u2019est le cas avec les m\u00e9thodes QuEChERS, les m\u00e9thodes ILSP minimisent le risque de perte d\u2019analyte pendant la purification. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Rendements ILSP pour divers produits alimentaires<\/h3>\n\n\n\n<p>Quasiment tous les pesticides \u00e9tudi\u00e9s ont \u00e9t\u00e9 r\u00e9cup\u00e9r\u00e9s et retrouv\u00e9s dans les six diff\u00e9rentes matrices, dans les crit\u00e8res de performance d\u00e9crits par les directives SANTE\/12682\/2019, en utilisant une cha\u00eene LC-MS\/MS de sensibilit\u00e9 faible \u00e0 moyenne (Tableau III). Ces r\u00e9sultats d\u00e9montrent l\u2019\u00e9norme potentiel de la pr\u00e9paration d\u2019\u00e9chantillons en ligne Revive pour l\u2019analyse multi-r\u00e9sidus de pesticides dans les denr\u00e9es alimentaires et les aliments pour animaux.<\/p>\n\n\n\n<p>De plus, lorsque la technologie Revive ILSP a \u00e9t\u00e9 compar\u00e9e \u00e0 des m\u00e9thodes QuEChERS analogues, l\u2019ILSP a donn\u00e9 des r\u00e9sultats \u00e0 minima comparables sinon meilleurs, dans tous les cas \u00e9tudi\u00e9s. Et dans les cas o\u00f9 certains compos\u00e9s tombent hors de la gamme id\u00e9ale de rendement de 70-120%, les directives telles que SANTE\/12682\/2019 pr\u00e9voient des dispositions pour rapporter ces donn\u00e9es, si les r\u00e9sultats sont robustes et r\u00e9p\u00e9tables comme c\u2019est le cas avec la purification automatis\u00e9e et reproductible ILSP.<\/p>\n\n\n\n<p><strong>Tableau III :<\/strong>&nbsp;<strong>Rendements et performance des m\u00e9thodes Revive ILSP d\u00e9velopp\u00e9es pour six produits alimentaires diff\u00e9rents<\/strong>.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Produit alimentaire<\/strong><\/td><td><strong>Pourcentage des compos\u00e9s avec rendements entre 70 et 120 % et RSD (concentration) \u2264 20 %<\/strong><\/td><\/tr><tr><td>Epinards<\/td><td>85.7% (5 ng\/g); 95.2% (100 ng\/g)<\/td><\/tr><tr><td>Orange enti\u00e8re<\/td><td>87% (10 ng\/g)<\/td><\/tr><tr><td>Plat \u00e0 base de soja<\/td><td>97% (10 ng\/g)<\/td><\/tr><tr><td>Avocat<\/td><td>95% (10 ng\/g)<\/td><\/tr><tr><td>Th\u00e9 \u00e0 l&#8217;hibiscus<\/td><td>92% (10 ng\/g)<\/td><\/tr><tr><td>Th\u00e9 noir<\/td><td>98% (10 ng\/g)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Effets de matrice &amp; ILSP<\/h3>\n\n\n\n<p>Comme pour toute analyse d\u2019\u00e9chantillons r\u00e9els, certains compos\u00e9s de la matrice peuvent \u00eatre extraits avec les analytes cibles. A cet \u00e9gard, l\u2019ILSP n\u2019est pas diff\u00e9rente des autres techniques de pr\u00e9paration d\u2019\u00e9chantillons et l\u2019utilisation de standards de calibration adapt\u00e9s \u00e0 la matrice (&#8220;matrix-matched&#8221;) est le meilleur moyen de s\u2019assurer de quantifications pr\u00e9cises. Ceci est d\u2019autant plus vrai pour les compos\u00e9s \u00e9luant au d\u00e9but de l\u2019analyse l\u00e0 o\u00f9 les compos\u00e9s de la matrice non ou peu retenus sont les plus susceptibles d\u2019appara\u00eetre et de causer un gain ou une perte de signal. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<\/p>\n\n\n\n<p>En plus d\u2019affecter la quantification, les compos\u00e9s interf\u00e9rents provenant de la matrice peuvent \u00e9galement contaminer les instruments. Dans les m\u00e9thodes de pr\u00e9paration d\u2019\u00e9chantillons en ligne, l\u2019\u00e9chantillon inject\u00e9 est non purifi\u00e9, il est donc important de s\u2019assurer que des rin\u00e7ages internes et externes de l\u2019aiguille sont effectu\u00e9s entre chaque injection pour \u00e9viter la contamination entre \u00e9chantillons et emp\u00eacher l\u2019accumulation des compos\u00e9s qui pourrait entra\u00eener des temps d\u2019arr\u00eat machine pour &#8220;nettoyage&#8221;.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Pr\u00e9paration d\u2019\u00e9chantillons en ligne Revive : une approche plus rapide pour l\u2019analyse multi-r\u00e9sidus de pesticides dans les aliments<\/h2>\n\n\n\n<p>La pr\u00e9paration d\u2019\u00e9chantillons en ligne ILSP utilise la puissance de vos cha\u00eenes LC-MS\/MS pour automatiser la purification des \u00e9chantillons. Avec une cartouche Revive ILSP Pesticides, une vanne 6 voies, et une pompe isocratique ind\u00e9pendante, votre instrument peut \u00eatre transform\u00e9 en un outil analytique ultra-performant, combinant la purification de l\u2019\u00e9chantillon et son analyse en une seule et m\u00eame m\u00e9thode efficace. En int\u00e9grant Revive ILSP dans vos m\u00e9thodes actuelles d\u2019analyse multi-r\u00e9sidus de pesticides dans les aliments, vous r\u00e9duirez le temps de pr\u00e9paration de vos \u00e9chantillons, vous r\u00e9duirez vos co\u00fbts associ\u00e9s aux produits de pr\u00e9paration d\u2019\u00e9chantillons \u00e0 usage unique et vous r\u00e9duirez les erreurs potentielles li\u00e9es aux proc\u00e9dures manuelles.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h5 class=\"wp-block-heading\"><strong>R\u00e9f\u00e9rences<\/strong><\/h5>\n\n\n\n<ol class=\"wp-block-list\">\n<li>A. Lupo, R.L. Romesberg, X. Lu, Automated inline pigment removal for the analysis of pesticide residues in spinach by liquid chromatography tandem mass spectrometry, J. Chromatogr. A 1629 (2020) 461477.&nbsp;<a href=\"https:\/\/doi.org\/10.1016\/j.chroma.2020.461477\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.chroma.2020.461477<\/a><\/li>\n\n\n\n<li>EU Reference Laboratories for Residues of Pesticides, SANTE\/2019\/12682, Analytical quality control and method validation procedures for pesticide residues analysis in food and feed (2020).&nbsp;<a href=\"https:\/\/www.eurl-pesticides.eu\/userfiles\/file\/EurlALL\/AqcGuidance_SANTE_2019_12682.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.eurl-pesticides.eu\/userfiles\/file\/EurlALL\/AqcGuidance_SANTE_2019_12682.pdf<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>La pr\u00e9paration d&#8217;\u00e9chantillons en ligne Revive utilise un processus automatis\u00e9 et en ligne de purification puis d\u2019analyse de l\u2019\u00e9chantillon, ce qui acc\u00e9l\u00e8re consid\u00e9rablement l&#8217;analyse LC-MS\/MS des  multi-r\u00e9sidus de  pesticides  dans les aliments.<\/p>\n","protected":false},"author":11,"featured_media":7065,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[],"industries-application":[2214,2216],"post-badge":[],"resource-type":[],"product-library":[2473,2447],"resource-technique":[2318],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43626","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-fr","industries-application-industrie-agroalimentaire","industries-application-securite-alimentaire","product-library-preparation-dechantillons-in-line-ilsp","product-library-produits-pour-la-preparation-dechantillons","resource-technique-chromatographie-en-phase-liquide"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"industries-application":[{"value":2214,"label":"Industrie 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