{"id":43794,"date":"2025-10-03T21:40:57","date_gmt":"2025-10-03T21:40:57","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/methods-for-the-passivation-of-hplc-instruments-and-columns\/"},"modified":"2026-01-02T18:37:31","modified_gmt":"2026-01-02T18:37:31","slug":"methods-for-the-passivation-of-hplc-instruments-and-columns","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles\/gnot5225\/methods-for-the-passivation-of-hplc-instruments-and-columns","title":{"rendered":"Methods for the Passivation of HPLC Instruments and Columns"},"content":{"rendered":"\n

Author(s):<\/strong> Diego A. Lopez1<\/sup>, Jesse Bischof2<\/sup>
1. Restek Corporation, 2. SilcoTek Corporation<\/p>\n\n\n\n

Published By:<\/strong> LCGC North America<\/p>\n\n\n\n

Issue:<\/strong> 06, Volume 41<\/p>\n\n\n\n

Year of Publication:<\/strong> 2023<\/p>\n\n\n\n

Link:<\/strong> https:\/\/doi.org\/10.56530\/lcgc.na.pj7690e5<\/a><\/p>\n\n\n\n

Abstract:<\/strong> Metal-analyte interaction is a type of nonspecific adsorption (NSA) that occurs mainly between acidic analytes and active adsorptive sites present in the metal surfaces of the fluidic path of any chromatographic system. NSA represents a chromatographic challenge since it may lead to poor peak shape, low recovery, and poor performance from the high-performance liquid chromatography (HPLC) system. As a result, many solutions that circumvent and mitigate this type of secondary interaction have been published or commercialized in the last few years. Such solutions encompass swapping stainless steel components for non-reactive polymers, \u201cbiocompatible\u201d metals or alloys, the addition of additives into the mobile phase, pretreating the system with large amounts of sample or strong acids, and the use of coated stainless-steel materials. This article explores these solutions in more detail, particularly the recent adaptation and benefits of coated metal surfaces in LC.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Metal-analyte interaction is a type of nonspecific adsorption (NSA) that occurs mainly between acidic analytes and active adsorptive sites present in the metal surfaces of the fluidic path of any chromatographic system. This article explores how to mitigate this type of secondary interaction, particularly by using coated metal surfaces in LC columns.<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[568],"product-library":[2391,2373],"resource-technique":[2299],"ppma_author":[451,694],"class_list":["post-43794","post","type-post","status-publish","format-standard","hentry","category-articles","resource-type-third-party-peer-reviewed-publications","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"resource-type":[{"value":568,"label":"Third-Party & Peer Reviewed Publications"}],"product-library":[{"value":2391,"label":"LC Columns"},{"value":2373,"label":"Liquid Chromatography Products"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":false,"author_info":{"display_name":"Diego L\u00f3pez","author_link":"https:\/\/discover.restek.com\/fr\/author\/diego-lopez\/"},"comment_info":0,"category_info":[{"term_id":7,"name":"Articles","slug":"articles","term_group":0,"term_taxonomy_id":7,"taxonomy":"category","description":"","parent":0,"count":464,"filter":"raw","cat_ID":7,"category_count":464,"category_description":"","cat_name":"Articles","category_nicename":"articles","category_parent":0}],"tag_info":false,"authors":[{"term_id":451,"user_id":31,"is_guest":0,"slug":"diego-lopez","display_name":"Diego L\u00f3pez","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-lopez-diego.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-lopez-diego.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":694,"user_id":0,"is_guest":1,"slug":"jesse-bischof","display_name":"Jesse Bischof","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=43794"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43794\/revisions"}],"predecessor-version":[{"id":79178,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43794\/revisions\/79178"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=43794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=43794"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=43794"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=43794"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=43794"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=43794"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=43794"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=43794"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=43794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}