{"id":43822,"date":"2020-12-22T23:00:00","date_gmt":"2020-12-22T23:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/raptor-hilic-si-simplify-the-switch-to-hilic\/"},"modified":"2026-02-17T19:57:33","modified_gmt":"2026-02-17T19:57:33","slug":"raptor-hilic-si-simplify-the-switch-to-hilic","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles\/gnss2687\/raptor-hilic-si-simplify-the-switch-to-hilic","title":{"rendered":"Raptor HILIC-Si: Simplify the Switch to HILIC"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Retain polar compounds without ion-pairing reagents.<\/li>\n\n\n\n<li>2.7 \u03bcm Raptor core-shell particles provide the speed of SPP.<\/li>\n\n\n\n<li>Ideal for increasing sensitivity and selectivity in LC-MS analyses.<\/li>\n\n\n\n<li>Fully compatible with both HPLC and UHPLC.<\/li>\n<\/ul>\n\n\n\n<p>With Raptor LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or \u201ccore-shell\u201d particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Restek is extending the speed and reliability of Raptor to the HILIC realm with the addition of the Raptor HILIC-Si column.<\/p>\n\n\n\n<p>Hydrophilic-interaction chromatography (HILIC) [1] is an increasingly popular alternative to reversed-phase LC for challenging polar analytes because it provides better retention of water-soluble compounds that are separated by polar differences (Figure 1). The Raptor HILIC-Si column simplifies the switch to HILIC because it delivers rugged Raptor performance, provides SPP column speed for faster analyses than traditional FPP silica columns, retains polar compounds without ion-pairing reagents, and is fully reliable, efficient, and selective with LC-MS compatible mobile phases.<\/p>\n\n\n\n<p class=\"has-small-font-size\">1. A.J. Alpert, Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds, J. Chromatogr. 499 (1990) 177\u2013196.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Column Description:<\/h2>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"432\" height=\"72\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnss2687a-01.jpg\" alt=\"\" class=\"wp-image-12262\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnss2687a-01.jpg 432w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnss2687a-01-300x50.jpg 300w\" sizes=\"auto, (max-width: 432px) 100vw, 432px\" \/><\/figure>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<style>.kb-image43822_838d2d-ae.kb-image-is-ratio-size, .kb-image43822_838d2d-ae .kb-image-is-ratio-size{max-width:207px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43822_838d2d-ae.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43822_838d2d-ae .kb-image-is-ratio-size{align-self:unset;}.kb-image43822_838d2d-ae figure{max-width:207px;}.kb-image43822_838d2d-ae .image-is-svg, .kb-image43822_838d2d-ae .image-is-svg img{width:100%;}.kb-image43822_838d2d-ae .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43822_838d2d-ae\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"422\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-422x1024.jpg\" alt=\"\" class=\"kb-img wp-image-61581\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-422x1024.jpg 422w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-124x300.jpg 124w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-768x1863.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-633x1536.jpg 633w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-844x2048.jpg 844w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-gnss2687a-01-1-scaled.jpg 1055w\" sizes=\"auto, (max-width: 422px) 100vw, 422px\" \/><\/figure><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Pore Size:<\/h4>\n\n\n\n<p>90 \u00c5<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Particle:<\/h4>\n\n\n\n<p>2.7 \u03bcm superficially porous particle (SPP or \u201ccore-shell\u201d particle) silica<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Surface Area:<\/h4>\n\n\n\n<p>130 m<sup>2<\/sup>\/g<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">End-Cap:<\/h4>\n\n\n\n<p>No<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Carbon Load:<\/h4>\n\n\n\n<p>NA<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">USP Phase Code:<\/h4>\n\n\n\n<p>L3<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Phase Category:<\/h4>\n\n\n\n<p>Bare silica<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Ligand Type:<\/h4>\n\n\n\n<p>None<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Recommended Usage:<\/h4>\n\n\n\n<p>pH Range: 1.0\u20138.0<br>Maximum Temperature: 80 \u00b0C<br>Maximum Pressure:&nbsp;600 bar\/8700 psi<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Properties:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Compatible with both HPLC and UHPLC instruments.<\/li>\n\n\n\n<li>Restek\u2019s 2.7 \u03bcm core-shell particles provide Raptor&nbsp;performance and the speed of SPP<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Switch to a Raptor HILIC-Si LC column when:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Increased retention of small polar compounds<br>is needed.<\/li>\n\n\n\n<li>You want to avoid using ion-pairing reagents.<\/li>\n\n\n\n<li>You want retention and sensitivity for hydrophilic&nbsp;compounds by LC-MS.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Use HILIC when greater retention of polar analytes is needed.\u00a0In HILIC mode, the aqueous mobile phase is the strong (or eluting) solvent\u00a0versus the more familiar reversed-phase mode, where elution is\u00a0the result of the organic solvent strength.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43822_f4c241-4c .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43822_f4c241-4c\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"911\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-768x911.jpg\" alt=\"\" class=\"kb-img wp-image-72866\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-768x911.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-253x300.jpg 253w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-863x1024.jpg 863w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-1295x1536.jpg 1295w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-01-1-1727x2048.jpg 1727w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Raptor Technology Ensures Rugged, Reproducible Performance in the HILIC Realm<\/h2>\n\n\n\n<p>Raptor LC columns are well known for their rugged dependability, and the new Raptor HILIC-Si column brings the consistency of Raptor performance to HILIC. Lot to lot, column to column, and injection to injection, every Raptor HILIC-Si column gives a consistent performance that you can count on (Figures 2 and 3). Simplify your move to HILIC with the reliability of Raptor HILIC-Si columns.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Raptor HILIC-Si columns maintain efficiency at any dimension, even at operating pressures up to 575 bar so you can run at high linear velocities with confidence.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43822_3fba3a-96 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43822_3fba3a-96\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"473\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-1024x473.jpg\" alt=\"\" class=\"kb-img wp-image-72874\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-1024x473.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-300x138.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-768x354.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-1536x709.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnss2687a-02-1-2048x945.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n\n\n<p>All testing performed using 2.7 \u03bcm Raptor HILIC-Si columns.<\/p>\n\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong>\u00a0Strict quality control guarantees that rugged Raptor HILIC-Si columns provide reproducible results lot after lot and injection after injection.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-737659fae28b2853a59f4c921157bd00\" style=\"color:#68bd45\">Consistent Performance across Lots<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0690.png\" alt=\"Raptor HILIC-Si Lot Comparison Metanephrine, Normetanephrine, and 3-Methoxytyramine Analysis\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0690<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3-Methoxytyramine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">151.00<\/td><td class=\"oth\">119.00<\/td><td class=\"oth\">91.02<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Metanephrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5001-33-2\/Metanephrine\" target=\"_blank\" rel=\"noopener\">Metanephrine<\/a><\/td><td class=\"oth\">1<\/td><td class=\"oth\">179.94<\/td><td class=\"oth\">148.22<\/td><td class=\"oth\">165.01<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">Normetanephrine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">166.00<\/td><td class=\"oth\">134.02<\/td><td class=\"oth\">121.01<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0690\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase A:mobile phase B (10:90)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>1 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 100 mM ammonium formate, pH 3.0 <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>5.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>80 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0690.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-bc7d9adb618eea331a1eb0f6e500e83f\" style=\"color:#68bd45\">Stable Results Injection after Injection<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0701.png\" alt=\"Raptor HILIC-Si Injection Consistency for Metanephrine, Normetanephrine, and 3-Methoxytyramine\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0701<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">1st Injection (t<sub>R<\/sub>)<\/th><th style=\"text-align: center;width: 75px\">135th Injection (t<sub>R<\/sub>)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3-Methoxytyramine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">151.00<\/td><td class=\"oth\">119.00<\/td><td class=\"oth\">91.02<\/td><td class=\"oth\">1.80<\/td><td class=\"oth\">1.83<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Metanephrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5001-33-2\/Metanephrine\" target=\"_blank\" rel=\"noopener\">Metanephrine<\/a><\/td><td class=\"oth\">1<\/td><td class=\"oth\">179.94<\/td><td class=\"oth\">148.22<\/td><td class=\"oth\">165.01<\/td><td class=\"oth\">2.03<\/td><td class=\"oth\">2.07<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">Normetanephrine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">166.00<\/td><td class=\"oth\">134.02<\/td><td class=\"oth\">121.01<\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">2.15<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0701\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase A:mobile phase B (10:90)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>1 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 100 mM ammonium formate, pH 3.0 <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>5.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0701.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Raptor SPP + HILIC = More Speed, More Sensitivity<\/h2>\n\n\n\n<p>What makes Raptor HILIC-Si columns special? The answer is simple: you get the speed of a Raptor SPP column with the unique separating power of the HILIC retention mechanism. The benefits of superficially porous particles (SPP) are well known. SPP columns are characterized by a layer of porous silica bonded to a solid silica core, which gives faster, more efficient analyses. As shown in Figure 4, when you keep instrument parameters constant (flow, gradient, temperature) and compare a 3 \u03bcm fully porous particle (FPP) silica column to a 2.7 \u03bcm Raptor HILIC-Si SPP column, the benefits become clear. Raptor HILIC-Si columns combine faster analysis times with higher sensitivity so you can increase sample throughput and lower limits of quantification (LOQs) for difficult-to-retain polar analytes.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4:<\/strong>\u00a0Raptor HILIC-Si columns provide the speed of SPP so you can analyze more samples per day.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-61a661f20be85d73e2fee1c36d26d878\" style=\"color:#68bd45\">The Raptor HILIC-Si Advantage<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>30% Decrease in elution time = increased sample throughput<\/li>\n\n\n\n<li>Elution in high % organic for efficient desolvation<\/li>\n\n\n\n<li>Narrower peaks = higher response, lower LOQs<\/li>\n\n\n\n<li>Excellent retention of difficult polar analytes<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0693.png\" alt=\"SPP vs. FPP Comparison: Metanephrine, Normetanephrine, and 3-Methoxytyramine\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0693<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precusor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Raptor t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Typical FPP t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3-Methoxytyramine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">151.00<\/td><td class=\"oth\">119.00<\/td><td class=\"oth\">1.84<\/td><td class=\"oth\">2.64<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Metanephrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5001-33-2\/Metanephrine\" target=\"_blank\" rel=\"noopener\">Metanephrine<\/a><\/td><td class=\"oth\">1<\/td><td class=\"oth\">179.94<\/td><td class=\"oth\">148.22<\/td><td class=\"oth\">2.08<\/td><td class=\"oth\">2.96<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">Normetanephrine<\/td><td class=\"oth\">1<\/td><td class=\"oth\">166.00<\/td><td class=\"oth\">134.02<\/td><td class=\"oth\">2.16<\/td><td class=\"oth\">3.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0693\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase A:mobile phase B (10:90)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>1 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 100 mM ammonium formate, pH 3.0 <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>5.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0693.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Raptor HILIC-Si Performance Gains vs. RP: Say Good-bye to Ion-Pairing Reagents<\/h2>\n\n\n\n<p>HILIC methods are becoming more common as analysts search for better solutions to challenging reversed-phase (RP) analyses. But, when is HILIC a beneficial alternative to a standard RP approach? HILIC should be considered when analyzing small polar compounds that are difficult to retain in RP mode without the use of ion-pairing reagents in the mobile phase. For example, paraquat and diquat are highly charged quaternary amine herbicides that are often analyzed in RP mode using ion-pairing reagents (Figure 5). But, these reagents can contaminate your LC-MS\/MS and require the system be taken off-line frequently for extensive cleaning. With Raptor HILIC-Si columns, ion-pairing reagents are not needed and paraquat and diquat are quickly retained and resolved with MS-friendly solvents and buffers so your instrument stays up and running longer (Figure 6).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 5:<\/strong>\u00a0RP Analysis of Paraquat and Diquat with Ion-Pairing Mobile Phase Reagent<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-38f16c9a37eff9c47ccfc9e8dcab74d3\" style=\"color:#68bd45\">50 ng Injected<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0383.png\" alt=\"Paraquat and Diquat on Fully Porous Reversed-Phase Quat Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0383<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion (amu)<\/th><th style=\"text-align: center;width: 75px\">Fragment Ion (amu)<\/th><th style=\"text-align: center;width: 75px\">DP (V)<\/th><th style=\"text-align: center;width: 75px\">Collision Energy (eV)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2761-72-9\/Diquat\" target=\"_blank\" rel=\"noopener\">Diquat<\/a><\/td><td class=\"oth\">183<\/td><td class=\"oth\">157<\/td><td class=\"oth\">30<\/td><td class=\"oth\">30<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Paraquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/4685-14-7\/Paraquat\" target=\"_blank\" rel=\"noopener\">Paraquat<\/a><\/td><td class=\"oth\">185<\/td><td class=\"oth\">170<\/td><td class=\"oth\">20<\/td><td class=\"oth\">20<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Fully Porous Reversed-Phase Quat Column<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>3 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>ambient<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>DI Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5 \u00b5g\/mL each component<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>10 mM heptafluorobutyric acid:acetonitrile (95:5)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.3 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Applied Biosystems\/MDS Sciex LC\/MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Model #:<\/th><td>API 3200\u2122 MS\/MS system <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Spray Voltage:<\/th><td>5.5 kV<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Curtain Gas:<\/th><td>15  psi (103.4 kPa)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Gas 1:<\/th><td>70  psi (482.6 kPa)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Gas 2:<\/th><td>60  psi (413.7 kPa)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Source Temp.:<\/th><td>600 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dwell Time:<\/th><td>200 ms<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Applied Biosystems\/MDS Sciex LC-MS\/MS System<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Collision exit potential:  3V<br \/>Q1\/Q3:  unit resolution<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Acknowledgement<\/th><td>Data courtesy of Houssain El Aribi, Ph.D., LC-MS Product and Application Specialist, MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0383.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 6:<\/strong>\u00a0Raptor HILIC-Si Analysis of Paraquat and Diquat with MS-Friendly Mobile Phases<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-4249a4b72db7f4cbe18c4039733b0857\" style=\"color:#68bd45\">0.25 ng Injected<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0512.png\" alt=\"Paraquat and Diquat on Raptor HILIC-Si\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0512<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/6385-62-2\/Diquat\" target=\"_blank\" rel=\"noopener\">Diquat<\/a><\/td><td class=\"oth\">3.25<\/td><td class=\"oth\">183.3<\/td><td class=\"oth\">157.2<\/td><td class=\"oth\">183.3<\/td><td class=\"oth\">130.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Paraquat\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1910-42-5\/Paraquat\" target=\"_blank\" rel=\"noopener\">Paraquat<\/a><\/td><td class=\"oth\">3.46<\/td><td class=\"oth\">185.3<\/td><td class=\"oth\">170.3<\/td><td class=\"oth\">171.3<\/td><td class=\"oth\">77.2<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0512\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>45 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Paraquat &amp; diquat calibration mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/32437?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0512\" rel=\"noopener\">cat.# 32437<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase B<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 50 mM ammonium formate, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>25:75 Water:acetonitrile, 50 mM ammonium formate, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>4.00<\/td><td>0.6<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>4.01<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>7.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0512.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Raptor HILIC-Si Improves the Performance of Your LC-MS\/MS<\/h2>\n\n\n\n<p>One of the most striking ways that HILIC analyses differ from standard RP analyses is that in HILIC methods the aqueous mobile phase is the stronger solvent. It provides differential selectivity that helps retain small polar analytes until after the sample matrix has eluted. In addition, the higher concentration organic mobile phases used in HILIC methods improve solvent evaporation during electrospray ionization, leading to increased sensitivity for LC-MS\/MS methods. The following applications illustrate the decrease in matrix interference and increase in LC-MS\/MS sensitivity that can be obtained using HILIC conditions and a Raptor HILIC-Si column.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Folic Acid and Metabolites<\/h3>\n\n\n\n<p>Folate deficiency is considered a risk factor for a wide range of human health problems including neural tube defects in newborns, cardiovascular diseases, Alzheimer\u2019s disease, and certain forms of cancer. The levels of folic acid and its metabolites in plasma are used as biomarkers to diagnose folate deficiency. However, when folic acid and its metabolites are extracted from plasma, phospholipids are also extracted and can cause matrix effects that make accurate quantitation difficult. Even with a good sample preparation protocol, 100% removal of phospholipids is difficult, and even at low levels they can interfere with the target analytes and also contaminate the MS source. By switching to a HILIC separation with a Raptor HILIC-Si column, you can quickly and easily separate the matrix components from the target analytes and accurately quantitate these important biomarkers for folate deficiency (Figure 7).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 7:<\/strong>\u00a0Folate Deficiency Biomarkers in Human Plasma<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-068975bfb11c66254e688d5319fd4e3b\" style=\"color:#68bd45\">More resolution between analytes and matrix components lets you divert matrix to waste, which keeps your MS cleaner longer.<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0698.png\" alt=\"Folate Deficiency Biomarkers in Human Plasma on Raptor HILIC-Si by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0698<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">5-Formyl tetrahydrofolate<\/td><td class=\"oth\">2.23<\/td><td class=\"oth\">50<\/td><td class=\"oth\">474.2<\/td><td class=\"oth\">327.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Folic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-30-3\/Folic acid\" target=\"_blank\" rel=\"noopener\">Folic acid<\/a><\/td><td class=\"oth\">2.23<\/td><td class=\"oth\">25<\/td><td class=\"oth\">442.2<\/td><td class=\"oth\">295.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">5-Methyltetrahydrofolic acid<\/td><td class=\"oth\">2.25<\/td><td class=\"oth\">25<\/td><td class=\"oth\">460.3<\/td><td class=\"oth\">313.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A5E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0698\" rel=\"noopener\">cat.# 9310A5E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>20 mM Ammonium acetate in acetonitrile:water (80:20) containing 10 mg\/mL 2-mercaptoethanol<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>50:50 Water:acetonitrile, 20 mM ammonium acetate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>20:80 Water:acetonitrile, 20 mM ammonium acetate <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>100<\/td><\/tr><tr><td>3.00<\/td><td>0.5<\/td><td>0<\/td><\/tr><tr><td>3.20<\/td><td>0.5<\/td><td>0<\/td><\/tr><tr><td>3.21<\/td><td>0.5<\/td><td>100<\/td><\/tr><tr><td>5.21<\/td><td>0.5<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>344 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>1. Aliquot 380 \u03bcL of human plasma (K2EDTA, 2x charcoal stripped) containing 100 \u03bcg\/mL 2-mercaptoethanol and add 20 \u03bcL fortification solution. <br \/>2. Vortex for 2 min and centrifuge for 2 min at 4000 rpm.<br \/>3. Condition EVOLUTE EXPRESS WAX 30 mg SPE plate (Biotage 604-0030-PX01) with 1 mL methanol and then equilibrate with 1 mL 2% formic acid in water. Apply vacuum to dry the plate completely.<br \/>4. Load 400 \u03bcL of sample onto the plate and apply vacuum to initiate the flow.<br \/>5. Wash the plate with 1 mL water. Apply vacuum to dry the plate completely.<br \/>6. Elute samples with 300 \u03bcL 5% ammonium hydroxide in methanol containing 10 mg\/mL 2-mercaptoethanol. Apply vacuum for elution.<br \/>7. Evaporate extracts to dryness under nitrogen at 30 \u00b0C.<br \/>8. Reconstitute in 200 \u03bcL mobile phase B containing 10 mg\/mL 2-mercaptoethanol.<br \/><br \/>Note: The whole sample preparation process was performed under dim light.<br \/><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Endogenous peaks for phospholipids and lysophospholipids are displayed because they are common matrix interferences and are known to suppress ionization efficiency.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0698.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Monoamine Neurotransmitters and Metabolites<\/h3>\n\n\n\n<p>Measurements of monoamine neurotransmitters and their metabolites in plasma and urine are commonly used for clinical diagnosis and monitoring of neuroblastoma and pheochromocytoma. Quantifying free metanephrine and normetanephrine is the most sensitive and accurate test for this purpose, but analysis of these polar compounds using reversed-phase LC is problematic due to very limited retention and poor sensitivity. As shown in Figure 8, these polar metabolites can be adequately retained on a Raptor HILIC-Si column and detected at 50 pg\/mL in human plasma, providing the sensitivity needed for clinical purposes (Figure 8).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 8:<\/strong>\u00a0Trace-Level Metanephrine, Normetanephrine, and 3-Methoxytyramine in Human Plasma<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-c3d3502af2cb144b014ea35b1337fb48\" style=\"color:#68bd45\">Even at 50 pg\/mL, good signal-to-noise ratios mean lower LOQs and accurate results at trace levels.<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0689.png\" alt=\"Metanephrine, Normetanephrine, and 3-Methoxytyramine in Human Plasma on Raptor HILIC-Si\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0689<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc. (pg\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3-Methoxytyramine-d4 (IS)<\/td><td class=\"oth\">1.80<\/td><td class=\"oth\">400<\/td><td class=\"oth\">155.07<\/td><td class=\"oth\">122.93<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">3-Methoxytyramine<\/td><td class=\"oth\">1.80<\/td><td class=\"oth\">50<\/td><td class=\"oth\">151.00<\/td><td class=\"oth\">119.00<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">Metanephrine-d3 (IS)<\/td><td class=\"oth\">2.03<\/td><td class=\"oth\">200<\/td><td class=\"oth\">183.00<\/td><td class=\"oth\">151.15<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Metanephrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5001-33-2\/Metanephrine\" target=\"_blank\" rel=\"noopener\">Metanephrine<\/a><\/td><td class=\"oth\">2.03<\/td><td class=\"oth\">50<\/td><td class=\"oth\">179.94<\/td><td class=\"oth\">148.22<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">Normetanephrine-d3 (IS)<\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">400<\/td><td class=\"oth\">169.00<\/td><td class=\"oth\">136.96<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Normetanephrine<\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">50<\/td><td class=\"oth\">166.00<\/td><td class=\"oth\">134.02<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0689\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Mobile phase A:mobile phase B (10:90)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 100 mM ammonium formate, pH 3.0 <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>5.00<\/td><td>0.3<\/td><td>10<\/td><td>90<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Charcoal stripped human plasma was fortified with analytes at 50 pg\/mL (0.25, 0.27, and 0.30 nmol\/L for metanephrine, normetanephrine, and 3-methoxytyramine, respectively). Internal standard (IS) was prepared at 4 ng\/mL for metanephrine-d3 and at 8 ng\/mL for normetanephrine-d3 and 3-methoxytyramine-d4 in methanol. The plasma sample (200 \u03bcL) was mixed with 10 \u03bcL of IS solution and 600  \u03bcL of 50 mM ammonium acetate solution. The mixture was loaded in an EVOLUTE EXPRESS WCX 96-well plate (30 mg) and washed with 1 mL water and 1 mL methanol:acetonitrile (50:50). The elution was performed twice with 0.9 mL of 5% formic acid in methanol:acetonitrile (50:50) and evaporated to dryness at 55 \u00b0C under a gentle stream of nitrogen. Dried extract was reconstituted with 100 \u03bcL of diluent and injected (10 \u03bcL) for analysis.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0689.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Food Adulterants<\/h3>\n\n\n\n<p>Foods that contain a high protein content command a higher price, which can result in the illegal practice of food adulteration using nitrogen-rich compounds, such as melamine, to make the protein content appear higher than the actual value. Due to its potential for toxicity, testing for melamine and other structurally related compounds is required in many countries for foods, feed materials, and pharmaceutical components. The method shown in Figure 9 provides excellent retention of these highly polar analytes with a separation time of only 3.5 minutes and a complete cycle time of just 8 minutes using a Raptor HILIC-Si column.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 9:<\/strong>\u00a0Food Adulterants on Raptor HILIC-Si<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-1082e40b7cd2871be7a27a0f4e36a130\" style=\"color:#68bd45\">The Raptor HILIC-Si column simplifies the analysis of difficult-to-retain polar analytes, such as melamine and related compounds.<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0515.png\" alt=\"Food Adulterants on Raptor HILIC-Si\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0515<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Polarity<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cyanuric acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108-80-5\/Cyanuric acid\" target=\"_blank\" rel=\"noopener\">Cyanuric acid<\/a><\/td><td class=\"oth\">0.47<\/td><td class=\"oth\">127.8<\/td><td class=\"oth\">84.9<\/td><td class=\"oth\">42.1<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cyromazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66215-27-8\/Cyromazine\" target=\"_blank\" rel=\"noopener\">Cyromazine<\/a><\/td><td class=\"oth\">0.52<\/td><td class=\"oth\">167.0<\/td><td class=\"oth\">68.2<\/td><td class=\"oth\">85.1<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Melamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108-78-1\/Melamine\" target=\"_blank\" rel=\"noopener\">Melamine<\/a><\/td><td class=\"oth\">0.89<\/td><td class=\"oth\">127.2<\/td><td class=\"oth\">85.0<\/td><td class=\"oth\">68.3<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammelide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/645-93-2\/Ammelide\" target=\"_blank\" rel=\"noopener\">Ammelide<\/a><\/td><td class=\"oth\">2.18<\/td><td class=\"oth\">129.1<\/td><td class=\"oth\">86.1<\/td><td class=\"oth\">70.2<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammeline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/645-92-1\/Ammeline\" target=\"_blank\" rel=\"noopener\">Ammeline<\/a><\/td><td class=\"oth\">2.97<\/td><td class=\"oth\">128.2<\/td><td class=\"oth\">86.2<\/td><td class=\"oth\">69.1<\/td><td class=\"oth\">+<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor HILIC-Si  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9310A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0515\" rel=\"noopener\">cat.# 9310A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>5:95 Water:acetonitrile, 10 mM ammonium formate, 0.1% formic acid<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 10 mM ammonium formate, 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>5:95 Water:acetonitrile, 10 mM ammonium formate, 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>0.50<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>3.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>3.51<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>8.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+\/ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>Scheduled MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0515.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Where\u2019s my 5 \u03bcm column?<\/h3>\n\n\n\n<p>Because HILIC methods use highly organic mobile phases, they generate very low backpressures. We\u2019ve simplified your move to HILIC by offering the Raptor HILIC-Si column in a 2.7 \u03bcm particle size only. Our testing and applications development demonstrated that 2.7 \u03bcm columns offer higher efficiency than 5 \u03bcm columns, and they are compatible with any HPLC or UHPLC instrument you have in your lab.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Experience <em>Selectivity Accelerated<\/em>. Order the Raptor HILIC-Si LC Column today.<\/h2>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Related Products<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9310A52\">                                Catalog No. 9310A52                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonne HPLC Raptor HILIC-Si, 2.7\u00b5m, 50 x 2.1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n             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class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9310A0252\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25937\">                                Catalog No. 25937                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">\u00c9crou EXP \u00e0 t\u00eate molet\u00e9e (\u00e9crou + ferrule)<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25937\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25808\">                                Catalog No. 25808                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Support de cartouche \u00e0 connexion directe EXP, livr\u00e9 avec un \u00e9crou et 2 ferrules<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25808\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Raptor HILIC-Si column simplifies the switch to HILIC because it delivers rugged Raptor performance, provides SPP column speed for faster analyses, ensures retention of polar compounds without ion pairing reagents, and is fully reliable, efficient, and selective with LC-MS compatible mobile phases.<\/p>\n","protected":false},"author":11,"featured_media":7399,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2391,2373],"resource-technique":[2297,2299,2301],"ppma_author":[414],"class_list":["post-43822","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-hilic","resource-technique-liquid-chromatography","resource-technique-mass-spectrometry-ms"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"product-library":[{"value":2391,"label":"LC 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