{"id":43835,"date":"2021-11-11T00:00:00","date_gmt":"2021-11-11T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/raptor-polar-x-analysez-une-large-gamme-de-composes-polaires-a-laide-dune-nouvelle-phase-stationnaire-hybride\/"},"modified":"2026-01-28T21:28:13","modified_gmt":"2026-01-28T21:28:13","slug":"raptor-polar-x-analysez-une-large-gamme-de-composes-polaires-a-laide-dune-nouvelle-phase-stationnaire-hybride","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-fr\/gnss3195-fr\/raptor-polar-x-analysez-une-large-gamme-de-composes-polaires-a-laide-dune-nouvelle-phase-stationnaire-hybride","title":{"rendered":"Raptor Polar X : analysez une large gamme de compos\u00e9s polaires \u00e0 l\u2019aide d\u2019une nouvelle phase stationnaire hybride"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Pas de d\u00e9rivation chronophage, ni technique complexe d\u2019appariement d\u2019ions.<\/li>\n\n\n\n<li>Basculez entre les modes de r\u00e9tention HILIC et \u00c9change Ionique par de simples changements de phase mobile et avec de courts temps d\u2019\u00e9quilibration.<\/li>\n\n\n\n<li>Id\u00e9ales pour am\u00e9liorer la s\u00e9lectivit\u00e9 et la sensibilit\u00e9 des analyses LC-MS.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Simplifiez vos analyses de compos\u00e9s polaires<\/h2>\n\n\n\n<p>Analyser des compos\u00e9s polaires en chromatographie liquide a toujours \u00e9t\u00e9 un d\u00e9fi. Leur faible r\u00e9tention, leur forme de pics d\u00e9grad\u00e9e, le recours \u00e0 des phases mobiles complexes pas forc\u00e9ment compatibles avec la LC-MS\/MS, de longs temps d\u2019\u00e9quilibration, une faible sensibilit\u00e9 ou encore l\u2019utilisation de d\u00e9rivations chronophages sont autant de challenges qui impactent l\u2019efficacit\u00e9 et la productivit\u00e9 des laboratoires. Mais le d\u00e9veloppement d\u2019une nouvelle colonne sp\u00e9cialement con\u00e7ue pour l\u2019analyse d\u2019une large gamme de compos\u00e9s polaires permet aux analystes de s\u2019affranchir de ces contraintes, en profitant pleinement des capacit\u00e9s de leur \u00e9quipement.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Utilisez la v\u00e9ritable puissance de la chromatographie<\/h2>\n\n\n\n<p>L\u2019un des aspects les plus importants des performances d\u2019une m\u00e9thode, souvent n\u00e9glig\u00e9, est d\u2019utiliser la phase stationnaire ad\u00e9quate. Pour l\u2019analyse des compos\u00e9s polaires, les colonnes de phase inverse ne fournissent pas assez de r\u00e9tention sauf \u00e0 utiliser des phases mobiles complexes ou \u00e0 d\u00e9river l\u2019\u00e9chantillon pour compenser le manque d\u2019interactions entre les analytes et la colonne. Mais en faisant correspondre les analytes \u00e0 une phase stationnaire sp\u00e9cialement d\u00e9velopp\u00e9e pour leur analyse, vous pourrez \u00e9viter les proc\u00e9dures complexes de pr\u00e9paration d\u2019\u00e9chantillons, diminuer les risques d\u2019erreur et gagner en productivit\u00e9.<\/p>\n\n\n\n<p>La phase stationnaire de la colonne Raptor Polar X est sp\u00e9cialement con\u00e7ue pour retenir de fa\u00e7on s\u00e9lective les analytes polaires gr\u00e2ce \u00e0 2 m\u00e9canismes de r\u00e9tention combin\u00e9s. Cette phase hybride, unique, est id\u00e9ale pour l\u2019analyse d\u2019une grande vari\u00e9t\u00e9 de compos\u00e9s polaires, notamment lorsqu\u2019elle est coupl\u00e9e \u00e0 la spectrom\u00e9trie de masse. Simplifiez vos analyses de compos\u00e9s polaires gr\u00e2ce au pouvoir de r\u00e9solution des colonnes Restek Raptor Polar X.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Une double s\u00e9lectivit\u00e9 sur une seule et m\u00eame colonne<\/h2>\n\n\n\n<p>Les approches HILIC (Hydrophilic Interaction LIquid Chromatography) et \u00c9change Ionique sont 2 des m\u00e9canismes de r\u00e9tention les plus utilis\u00e9s pour retenir et analyser les compos\u00e9s polaires. Restek a d\u00e9velopp\u00e9 une nouvelle chimie de phase stationnaire conjuguant ces 2 m\u00e9canismes sur un seul et unique greffon. Ce greffon \u00e9tant combin\u00e9 \u00e0 des particules superficiellement poreuses, les colonnes Raptor Polar X retiennent et s\u00e9parent de mani\u00e8re fiable et efficace une large gamme de compos\u00e9s polaires.<\/p>\n\n\n\n<p>Les colonnes d\u00e9di\u00e9es \u00e0 une application favorisent souvent l\u2019un ou l\u2019autre des m\u00e9canismes et les am\u00e9liorations obtenues pour certains compos\u00e9s polaires se font au d\u00e9triment des autres. A l\u2019inverse, la chimie sp\u00e9cifique en cours de brevet de la colonne Raptor X permet l\u2019utilisation ind\u00e9pendante ou simultan\u00e9e des 2 modes disponibles, offrant une r\u00e9tention optimale et ajustable (Figure 1). De simples modifications des conditions de phase mobile permettent de basculer d\u2019un mode \u00e0 l\u2019autre, pour une r\u00e9tention s\u00e9lective des compos\u00e9s d\u2019int\u00e9r\u00eat, sans avoir besoin de longs temps d\u2019\u00e9quilibration au d\u00e9but et entre chaque analyse.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1 :<\/strong>\u00a0La colonne Raptor Polar X peut rapidement basculer entre diff\u00e9rents modes de r\u00e9tention par de simples modifications des conditions de phase mobile, offrant la possibilit\u00e9 de retenir et s\u00e9parer une grande vari\u00e9t\u00e9 de compos\u00e9s polaires, en une unique analyse.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"826\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-01-1024x826.jpg\" alt=\"\" class=\"wp-image-23304\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1024x826.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-300x242.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-768x620.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1536x1239.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Comment passer d\u2019un mode de r\u00e9tention \u00e0 l\u2019autre ?<\/h2>\n\n\n\n<p>Lorsque l\u2019on utilise une phase mobile contenant un fort pourcentage d\u2019ac\u00e9tonitrile et un faible pourcentage d\u2019eau, une couche aqueuse se forme \u00e0 la surface de la silice dans laquelle les compos\u00e9s polaires peuvent interagir avec le ligand greff\u00e9 \u00e0 la surface de la silice (Figure 2). La sp\u00e9cificit\u00e9 du greffon de la nouvelle colonne Raptor Polar X simplifie ces m\u00e9canismes, permettant des mises en route et des temps d\u2019\u00e9quilibration plus rapides que jamais. Cela signifie que les colonnes sont pr\u00eates \u00e0 \u00eatre utilis\u00e9es tr\u00e8s rapidement, elles permettent ainsi d\u2019augmenter la cadence des analyses\/<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2 :<\/strong>\u00a0La formation rapide d\u2019une couche aqueuse \u00e0 la surface de la particule de silice permet \u00e0 une grande vari\u00e9t\u00e9 de compos\u00e9s polaires de partitionner entre l\u2019ac\u00e9tonitrile pr\u00e9sent dans la phase mobile et la couche aqueuse cr\u00e9\u00e9e. Ce ph\u00e9nom\u00e8ne de partage associ\u00e9 aux interactions avec la phase stationnaire, d\u00e9finit le mode de r\u00e9tention HILIC.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"531\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-gnss3195-02_fr-1024x531.jpg\" alt=\"\" class=\"wp-image-23316\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-gnss3195-02_fr-1024x531.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-gnss3195-02_fr-300x156.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-gnss3195-02_fr-768x398.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-gnss3195-02_fr-1536x797.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-gnss3195-02_fr-2048x1062.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<p>Le pourcentage \u00e9lev\u00e9 d\u2019ac\u00e9tonitrile dans la phase mobile g\u00e9n\u00e8re de fortes interactions entre la phase stationnaire et les petits analytes polaires et charg\u00e9s, offrant une excellente r\u00e9tention de ces compos\u00e9s. Pour affiner cette r\u00e9tention, il suffit d\u2019augmenter la quantit\u00e9 de phase aqueuse dans la phase mobile. Les compos\u00e9s polaires et charg\u00e9s, entra\u00een\u00e9s par la phase mobile, seront alors \u00e9lu\u00e9s. Avec l\u2019utilisation d\u2019une phase mobile contenant un pourcentage de phase aqueuse plus important, la r\u00e9tention due au m\u00e9canisme HILIC diminue et les caract\u00e9ristiques d\u2019\u00c9change Ionique de la phase stationnaire deviennent pr\u00e9pond\u00e9rantes, favorisant un m\u00e9canisme de r\u00e9tention par \u00c9change Ionique (Figure 3).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3 :<\/strong>\u00a0Dans des conditions de phase mobile \u00e0 fort pourcentage de phase aqueuse, le m\u00e9canisme d\u2019\u00c9change Ionique devient le mode de r\u00e9tention pr\u00e9pond\u00e9rant dans l\u2019analyse des compos\u00e9s polaires.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"489\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-03-1024x489.jpg\" alt=\"\" class=\"wp-image-23328\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1024x489.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-300x143.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-768x367.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1536x734.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Exemple de passage d\u2019un mode de r\u00e9tention \u00e0 l\u2019autre<\/h2>\n\n\n\n<p>Afin d\u2019illustrer ces principes, la figure 4 montre comment deux types diff\u00e9rents de compos\u00e9s polaires interagissent avec la phase stationnaire, et comment leurs r\u00e9ponses peuvent \u00eatre modifi\u00e9es par de simples changements de phase mobile. Dans cet exemple, nous avons analys\u00e9 deux vitamines hydrosolubles : la vitamine B3 (niacine), un acide organique, et la vitamine B1 (thiamine) qui, elle, a une charge positive permanente.<\/p>\n\n\n\n<p>Sur le chromatogramme du haut, une phase mobile avec de forts pourcentages de solvant organique a \u00e9t\u00e9 utilis\u00e9e, favorisant le m\u00e9canisme de r\u00e9tention HILIC. En utilisant la colonne Raptor Polar X dans ces conditions, une couche aqueuse se forme rapidement \u00e0 la surface des particules de silice. Cela permet aux compos\u00e9s polaires pr\u00e9sents de se r\u00e9partir entre la phase organique (ac\u00e9tonitrile) et la phase aqueuse o\u00f9 ils peuvent alors interagir avec la phase stationnaire. Les compos\u00e9s polaires sont retenus sur la phase stationnaire par des interactions hydrophiles mais restent n\u00e9anmoins \u00e9lu\u00e9s dans des conditions de phase mobile \u00e0 fort pourcentage de solvant organique. Les analyses LC-MS\/MS sont ici grandement favoris\u00e9es gr\u00e2ce \u00e0 une meilleure d\u00e9solvatation et une efficacit\u00e9 d\u2019ionisation accrue.<\/p>\n\n\n\n<p>Le chromatogramme du bas met en \u00e9vidence les modifications chromatographiques que l\u2019on peut observer lorsque l\u2019on exp\u00e9rimente un autre m\u00e9canisme de r\u00e9tention en utilisant une phase mobile avec un pourcentage de phase aqueuse plus important. Ces conditions vont favoriser le m\u00e9canisme de r\u00e9tention d\u2019\u00c9change Ionique au d\u00e9triment du mode HILIC, entrainant une perte de r\u00e9tention des vitamines B1 et B3. L\u2019ordre d\u2019\u00e9lution est \u00e9galement modifi\u00e9 dans ces conditions, la vitamine B1 \u00e9tant moins retenue que la vitamine B3 dans ces conditions d\u2019\u00c9change Ionique.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4 :<\/strong>\u00a0Par la modification de la composition des phases mobiles &#8211; simples \u00e0 r\u00e9aliser et totalement compatibles avec la LC-MS\/MS, il est possible de favoriser l\u2019un ou l\u2019autre des m\u00e9canismes de r\u00e9tention disponibles avec la nouvelle chimie de phase stationnaire des colonnes Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.png\" alt=\"Raptor Polar X Retention Mode Comparison: Buffered B Vitamins with 95% vs. 60% Mobile Phase B\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0652<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B3\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-67-6\/Vitamin B3\" target=\"_blank\" rel=\"noopener\">Vitamin B3<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-43-8\/Vitamin B1\" target=\"_blank\" rel=\"noopener\">Vitamin B1<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0652\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in acetonitrile<\/td><\/tr><td><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.5 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Mobile phase A: Water, 5 mM ammonium formate, 0.1% formic acid<br \/>Mobile phase B: Acetonitrile, 0.1% formic acid<br \/>Top chromatogram: 95% B, 10 min run, 2 \u03bcL injection (100 ppm B3, 0.01 ppm B1)<br \/>Bottom chromatogram: 60% B, 5 min run, 0.5 \u03bcL injection (100 ppm B3, 0.1 ppm B1)<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Une r\u00e9tention hybride vous permettant d\u2019analyser un spectre d\u2019analytes plus large avec une seule et m\u00eame m\u00e9thode<\/h2>\n\n\n\n<p>Les colonnes HPLC se concentrent g\u00e9n\u00e9ralement sur un seul type d\u2019interactions, au d\u00e9triment des autres. Cette approche est efficace lorsque les analytes ont des caract\u00e9ristiques similaires. Cependant, les compos\u00e9s polaires ayant des propri\u00e9t\u00e9s chimiques qui varient consid\u00e9rablement, cette strat\u00e9gie implique de recourir \u00e0 des m\u00e9thodes et des colonnes diff\u00e9rentes pour chaque type d\u2019analytes. Comme le montrent les exemples suivants, la chimie hybride de la phase stationnaire des colonnes Raptor Polar X est l\u2019alternative id\u00e9ale de par son profil de r\u00e9tention &#8220;multimode&#8221;, permettant l\u2019analyse d\u2019une grande vari\u00e9t\u00e9 de compos\u00e9s sur une seule et m\u00eame m\u00e9thode.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Liste de contaminants polaires &#8211; m\u00e9thode de type QuPPe<\/h2>\n\n\n\n<p>La m\u00e9thode europ\u00e9enne QuPPe (Quick Polar Pesticides) comprend des compos\u00e9s polaires allant d\u2019herbicides polaires anioniques, tels que le glyphosate et ses compos\u00e9s apparent\u00e9s, \u00e0 une vari\u00e9t\u00e9 de contaminants oxychlor\u00e9s comme le chlorate et le perchlorate. Comme le montre la figure 5, la colonne Raptor Polar X permet de s\u00e9parer rapidement ce m\u00e9lange complexe avec un cycle d\u2019analyse de seulement 13 minutes, le dernier compos\u00e9 \u00e9luant autour de 10.5 minutes. Cette s\u00e9paration est r\u00e9alis\u00e9e \u00e0 l\u2019aide de phases mobiles simples, non tamponn\u00e9es, seulement acidifi\u00e9es avec 0.5% d\u2019acide formique pour am\u00e9liorer la forme des pics. La m\u00e9thode illustr\u00e9e en figure5 permet \u00e9galement de s\u00e9parer des compos\u00e9s ayant les m\u00eames fragments en MS, comme l\u2019AMPA et le N-acetyl AMPA, ou le fosetyl aluminium et les acides phosponique et phosphorique (ce dernier \u00e9tant reconnu comme une source fr\u00e9quente d\u2019interf\u00e9rences analytiques).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 5 :\u00a0<\/strong>Dans cette analyse de compos\u00e9s polaires, un m\u00e9lange complexe d\u2019analytes peut \u00eatre totalement r\u00e9solu en une seule et m\u00eame m\u00e9thode gr\u00e2ce aux caract\u00e9ristiques uniques de r\u00e9tention de la colonne Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.png\" alt=\"17 Polar Pesticide Panel on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0546<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><th style=\"text-align: center;width: 75px\">Precursor 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Aminomethylphosphonic acid (AMPA)<\/td><td class=\"oth\">0.805<\/td><td class=\"oth\">200<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Bialophos<\/td><td class=\"oth\">0.847<\/td><td class=\"oth\">100<\/td><td class=\"oth\">322.2<\/td><td class=\"oth\">88.2<\/td><td class=\"oth\">216.1<\/td><td class=\"oth\">134.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perchlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14797-73-0\/Perchlorate\" target=\"_blank\" rel=\"noopener\">Perchlorate<\/a><\/td><td class=\"oth\">2.593<\/td><td class=\"oth\">5<\/td><td class=\"oth\">101.0<\/td><td class=\"oth\">84.95<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">98.9<\/td><td class=\"oth\">83<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">3.376<\/td><td class=\"oth\">200<\/td><td class=\"oth\">180.2<\/td><td class=\"oth\">85.2<\/td><td class=\"oth\">95.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">3-(Methylphophinico) propionic acid (MPPA)<\/td><td class=\"oth\">4.076<\/td><td class=\"oth\">100<\/td><td class=\"oth\">151.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">107.1<\/td><td class=\"oth\">133.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoracetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoracetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoracetic acid (TFA)<\/a><\/td><td class=\"oth\">4.423<\/td><td class=\"oth\">20<\/td><td class=\"oth\">113.0<\/td><td class=\"oth\">69.1<\/td><td class=\"oth\">19.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">2-Hydroxyethane phosphonic acid (HEPA)<\/td><td class=\"oth\">4.969<\/td><td class=\"oth\">100<\/td><td class=\"oth\">125.1<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">63<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Difluoroacetic acid (DFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/381-73-7\/Difluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Difluoroacetic acid (DFA)<\/a><\/td><td class=\"oth\">5.018<\/td><td class=\"oth\">200<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">51.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14866-68-3\/Chlorate\" target=\"_blank\" rel=\"noopener\">Chlorate<\/a><\/td><td class=\"oth\">5.542<\/td><td class=\"oth\">100<\/td><td class=\"oth\">85.0<\/td><td class=\"oth\">69.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">83.0<\/td><td class=\"oth\">67.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethephon\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/16672-87-0\/Ethephon\" target=\"_blank\" rel=\"noopener\">Ethephon<\/a><\/td><td class=\"oth\">5.564<\/td><td class=\"oth\">200<\/td><td class=\"oth\">143.1<\/td><td class=\"oth\">107.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">6.113<\/td><td class=\"oth\">200<\/td><td class=\"oth\">168.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\">Bromide<\/td><td class=\"oth\">6.423<\/td><td class=\"oth\">2000<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">79.0<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Bromate<\/td><td class=\"oth\">6.771<\/td><td class=\"oth\">600<\/td><td class=\"oth\">129.0<\/td><td class=\"oth\">113<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">127<\/td><td class=\"oth\">111.1<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\">N-acetyl AMPA<\/td><td class=\"oth\">6.932<\/td><td class=\"oth\">200<\/td><td class=\"oth\">152.1<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\">Fosetyl aluminum<\/td><td class=\"oth\">7.775<\/td><td class=\"oth\">80<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">81.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">78.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\">Phosphonic acid<\/td><td class=\"oth\">8.275<\/td><td class=\"oth\">500<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\">N-acetyl glufosinate<\/td><td class=\"oth\">9.980<\/td><td class=\"oth\">200<\/td><td class=\"oth\">222.2<\/td><td class=\"oth\">136.1<\/td><td class=\"oth\">134.1<\/td><td class=\"oth\">59.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0546\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>13<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p>En plus de la m\u00e9thode d\u00e9velopp\u00e9e par Restek, illustr\u00e9e en figure 5, les pr\u00e9colonnes et colonnes analytiques Raptor Polar X ont chacune \u00e9t\u00e9 \u00e9valu\u00e9es et incluses dans la m\u00e9thode QuPPe elle-m\u00eame [1]. Elles sont utilis\u00e9es dans une m\u00e9thode de d\u00e9tection par LC-MS\/MS en mode ESI n\u00e9gatif d\u2019une grande vari\u00e9t\u00e9 de pesticides polaires.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Acides amin\u00e9s non-d\u00e9riv\u00e9s<\/h2>\n\n\n\n<p>Les acides amin\u00e9s sont un groupe h\u00e9t\u00e9rog\u00e8ne de compos\u00e9s polaires, g\u00e9n\u00e9ralement analys\u00e9s en phase inverse ou par \u00c9change Ionique en utilisant des d\u00e9rivations pr\u00e9- ou post-colonne. L\u2019analyse directe des acides amin\u00e9s non-d\u00e9riv\u00e9s est difficile en raison d\u2019une faible r\u00e9tention et de performances chromatographiques m\u00e9diocres. Cependant, les acides amin\u00e9s non-d\u00e9riv\u00e9s avec des cha\u00eenes lat\u00e9rales polaires, non polaires, charg\u00e9es positivement ou n\u00e9gativement sont tous retenus et tr\u00e8s bien s\u00e9par\u00e9s sur une seule et m\u00eame m\u00e9thode avec la colonne Raptor Polar X. La figure 6 montre l\u2019analyse de 21 acides amin\u00e9s, non d\u00e9riv\u00e9s, incluant un suppl\u00e9ment de taurine, dans une pr\u00e9paration pour nourrissons, apr\u00e8s une simple pr\u00e9cipitation de prot\u00e9ines et analyse directe de l\u2019extrait.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 6 :<\/strong>\u00a0Des compos\u00e9s aux polarit\u00e9s vari\u00e9es, tels que les acides amin\u00e9s repr\u00e9sent\u00e9s, peuvent \u00eatre analys\u00e9s sur une m\u00eame m\u00e9thode avec la colonne Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.png\" alt=\"Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0579<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tryptophan\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54-12-6\/Tryptophan\" target=\"_blank\" rel=\"noopener\">Tryptophan<\/a><\/td><td class=\"oth\">1.17<\/td><td class=\"oth\">205.07<\/td><td class=\"oth\">146.08<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenylalanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-91-2\/Phenylalanine\" target=\"_blank\" rel=\"noopener\">Phenylalanine<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">166.13<\/td><td class=\"oth\">120.10<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Leucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-90-5\/Leucine\" target=\"_blank\" rel=\"noopener\">Leucine<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Isoleucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73-32-5\/Isoleucine\" target=\"_blank\" rel=\"noopener\">Isoleucine<\/a><\/td><td class=\"oth\">1.55<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methionine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-51-8\/Methionine\" target=\"_blank\" rel=\"noopener\">Methionine<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">150.07<\/td><td class=\"oth\">104.10<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tyrosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/556-03-6\/Tyrosine\" target=\"_blank\" rel=\"noopener\">Tyrosine<\/a><\/td><td class=\"oth\">1.69<\/td><td class=\"oth\">182.10<\/td><td class=\"oth\">136.08<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Taurine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/107-35-7\/Taurine\" target=\"_blank\" rel=\"noopener\">Taurine<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">126.07<\/td><td class=\"oth\">108.07<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Valine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/516-06-3\/Valine\" target=\"_blank\" rel=\"noopener\">Valine<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">118.13<\/td><td class=\"oth\">72.11<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Proline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/609-36-9\/Proline\" target=\"_blank\" rel=\"noopener\">Proline<\/a><\/td><td class=\"oth\">2.29<\/td><td class=\"oth\">116.13<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-72-7\/Alanine\" target=\"_blank\" rel=\"noopener\">Alanine<\/a><\/td><td class=\"oth\">3.00<\/td><td class=\"oth\">90.03<\/td><td class=\"oth\">44.10<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Threonine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-68-2\/Threonine\" target=\"_blank\" rel=\"noopener\">Threonine<\/a><\/td><td class=\"oth\">3.12<\/td><td class=\"oth\">120.13<\/td><td class=\"oth\">74.08<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glycine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-40-6\/Glycine\" target=\"_blank\" rel=\"noopener\">Glycine<\/a><\/td><td class=\"oth\">3.62<\/td><td class=\"oth\">76.10<\/td><td class=\"oth\">30.11<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-85-9\/Glutamine\" target=\"_blank\" rel=\"noopener\">Glutamine<\/a><\/td><td class=\"oth\">3.87<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.07<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Serine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-84-1\/Serine\" target=\"_blank\" rel=\"noopener\">Serine<\/a><\/td><td class=\"oth\">3.93<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">60.09<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Asparagine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70-47-3\/Asparagine\" target=\"_blank\" rel=\"noopener\">Asparagine<\/a><\/td><td class=\"oth\">4.08<\/td><td class=\"oth\">133.13<\/td><td class=\"oth\">74.07<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Arginine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/74-79-3\/Arginine\" target=\"_blank\" rel=\"noopener\">Arginine<\/a><\/td><td class=\"oth\">4.47<\/td><td class=\"oth\">175.17<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Histidine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-00-1\/Histidine\" target=\"_blank\" rel=\"noopener\">Histidine<\/a><\/td><td class=\"oth\">4.66<\/td><td class=\"oth\">156.07<\/td><td class=\"oth\">110.16<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lysine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-87-1\/Lysine\" target=\"_blank\" rel=\"noopener\">Lysine<\/a><\/td><td class=\"oth\">4.97<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.13<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-65-2\/Glutamic acid\" target=\"_blank\" rel=\"noopener\">Glutamic acid<\/a><\/td><td class=\"oth\">5.89<\/td><td class=\"oth\">148.10<\/td><td class=\"oth\">84.10<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cystine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-89-3\/Cystine\" target=\"_blank\" rel=\"noopener\">Cystine<\/a><\/td><td class=\"oth\">6.10<\/td><td class=\"oth\">241.13<\/td><td class=\"oth\">152.00<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aspartic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-45-8\/Aspartic acid\" target=\"_blank\" rel=\"noopener\">Aspartic acid<\/a><\/td><td class=\"oth\">7.12<\/td><td class=\"oth\">134.07<\/td><td class=\"oth\">74.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0579\" rel=\"noopener\">cat.# 9311A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>20:80 Water:acetonitrile, 0.01 N HCl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>  Endogenous amino acids<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Mobile Phase B: 9:1 Acetonitrile:20 mM ammonium formate in water (pH 3.0) (The ammonium formate concentration is 20 mM relative to the total volume of mobile phase B. See preparation notes for instructions on diluting a 200 mM aqueous starting solution.) <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>3.50<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>8.01<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>10.0<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A 200 \u00b5L aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 \u00b5L of acetonitrile and 10 \u00b5L of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><i>Mobile Phase B Preparation:<\/i> To make 500 mL of mobile phase B, measure ~45 mL of water into a small beaker and add 1 mL of 10 M ammonium formate solution. Adjust pH to 3.0 by adding formic acid and then bring the volume to 50 mL with water. Combine this 50 mL ammonium formate solution (pH 3.0) with 450 mL of acetonitrile to complete the preparation.   <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">PFAS \u00e0 cha\u00eenes ultra-courtes et cha\u00eenes longues<\/h2>\n\n\n\n<p>Le dernier exemple de la capacit\u00e9 de la colonne Raptor Polar X \u00e0 maximiser la productivit\u00e9 d\u2019une m\u00e9thode est une application qui ouvre la voie \u00e0 de nouveaux tests. Les m\u00e9thodes LC-MS\/MS actuelles pour l\u2019analyse des PFAS (&#8220;PolyFluoroAlkyl Substances&#8221;) se focalisent sur les cha\u00eenes courtes (C4 \u2013 C6), les cha\u00eenes longues (C8 et au-del\u00e0) et les compos\u00e9s alternatifs mais elles n\u2019incluent pas les compos\u00e9s \u00e9mergents \u00e0 cha\u00eenes ultra-courtes (C2 et C3). Les m\u00e9thodes actuelles en phase inverse ne fournissent pas assez de r\u00e9tention pour les PFAS \u00e0 cha\u00eenes ultra-courtes, tandis que les m\u00e9thodes d\u2019\u00e9change anionique en fournissent trop, avec pour r\u00e9sultat des performances chromatographiques m\u00e9diocres. Gr\u00e2ce au double m\u00e9canisme de r\u00e9tention de la colonne Raptor Polar X, les PFAS \u00e0 cha\u00eenes ultra-courtes et ceux \u00e0 cha\u00eenes longues peuvent tous \u00eatre analys\u00e9s sur une m\u00eame m\u00e9thode isocratique de 5 minutes (Figure 7).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 7 :<\/strong>\u00a0Les PFAS \u00e0 cha\u00eenes ultra-courtes, \u00e0 cha\u00eenes longues et alternatifs, sont tous analys\u00e9s sur une seule et m\u00eame m\u00e9thode gr\u00e2ce \u00e0 la nouvelle phase stationnaire de la colonne Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.png\" alt=\"Ultrashort- Through Long-Chain and Alternative PFAS on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11CL-PF3OUdS)<\/td><td class=\"oth\">1.25<\/td><td class=\"oth\">400<\/td><td class=\"oth\">630.78<\/td><td class=\"oth\">450.80<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)<\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">400<\/td><td class=\"oth\">530.78<\/td><td class=\"oth\">350.85<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">400<\/td><td class=\"oth\">498.84<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanesulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">400<\/td><td class=\"oth\">398.90<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanesulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanesulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">1.64<\/td><td class=\"oth\">400<\/td><td class=\"oth\">298.97<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoropropanesulfonic acid (PFPrS)<\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">400<\/td><td class=\"oth\">248.97<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">Perfluoroethanesulfonic acid (PFEtS)<\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">400<\/td><td class=\"oth\">198.98<\/td><td class=\"oth\">79.92<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hexafluoropropylene oxide dimer acid (HFPO-DA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13252-13-6\/Hexafluoropropylene oxide dimer acid\" target=\"_blank\" rel=\"noopener\">Hexafluoropropylene oxide dimer acid (HFPO-DA)<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">284.97<\/td><td class=\"oth\">168.92<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">400<\/td><td class=\"oth\">412.90<\/td><td class=\"oth\">368.91<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/919005-14-4\/Ammonium 4,8-dioxa-3H-perfluorononanoate\" target=\"_blank\" rel=\"noopener\">Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)<\/a><\/td><td class=\"oth\">2.15<\/td><td class=\"oth\">400<\/td><td class=\"oth\">376.90<\/td><td class=\"oth\">250.93<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">2.36<\/td><td class=\"oth\">400<\/td><td class=\"oth\">312.97<\/td><td class=\"oth\">268.90<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanoic acid (PFBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-22-4\/Perfluorobutanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanoic acid (PFBA)<\/a><\/td><td class=\"oth\">2.76<\/td><td class=\"oth\">400<\/td><td class=\"oth\">212.97<\/td><td class=\"oth\">168.97<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Perfluoropropionic acid (PFPrA)<\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">163.03<\/td><td class=\"oth\">119.01<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoroacetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoroacetic acid (TFA)<\/a><\/td><td class=\"oth\">3.77<\/td><td class=\"oth\">400<\/td><td class=\"oth\">113.03<\/td><td class=\"oth\">69.01<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0569\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>400  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 10 mM ammonium formate, 0.05% formic acid   <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>60:40 Acetonitrile:methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Passiver le syst\u00e8me : est-ce n\u00e9cessaire ?<\/h2>\n\n\n\n<p>Le glyphosate peut \u00eatre difficile \u00e0 analyser \u00e0 l\u2019\u00e9tat de traces car il se ch\u00e9late aux ions m\u00e9talliques pr\u00e9sents dans le syst\u00e8me LC. La colonne Raptor X est livr\u00e9e d\u00e9j\u00e0 trait\u00e9e et pr\u00eate \u00e0 l\u2019emploi, mais il peut \u00eatre utile de passiver les autres compos\u00e9s m\u00e9talliques de votre syst\u00e8me LC en contact avec l\u2019\u00e9chantillon. La n\u00e9cessit\u00e9 d\u2019une passivation d\u00e9pendra de l\u2019application et de la configuration de votre syst\u00e8me LC, mais pour l\u2019analyse de compos\u00e9s polaires connus pour se ch\u00e9later, tel que le glyphosate, Restek recommande de traiter votre syst\u00e8me avec une solution de passivation LC (r\u00e9f. 32475) avant d\u2019effectuer vos analyses.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Une nouvelle phase stationnaire d\u00e9velopp\u00e9e selon les crit\u00e8res de qualit\u00e9 stricts des colonnes de la gamme Raptor<\/h2>\n\n\n\n<p>La colonne Raptor Polar X a \u00e9t\u00e9 con\u00e7ue, fabriqu\u00e9e et test\u00e9e selon les m\u00eames standards rigoureux qui ont fait des colonnes Raptor un synonyme de qualit\u00e9. Qu\u2019il s\u2019agisse de leur r\u00e9p\u00e9tabilit\u00e9 sans faille (injection apr\u00e8s injection) lors d\u2019analyses d\u2019\u00e9chantillons r\u00e9els dans des matrices complexes comme les \u00e9pinards (figure 8), ou de leur parfaite reproductibilit\u00e9 (colonne \u00e0 colonne, lot \u00e0 lot) quand il est finalement temps de changer la colonne (figure 9), vous pouvez toujours compter sur la qualit\u00e9 de la gamme Raptor. De plus, le recours \u00e0 un ligand unique pour la r\u00e9tention hybride de la colonne Raptor Polar X permet de garantir que chaque colonne est fabriqu\u00e9e de fa\u00e7on uniforme et peut r\u00e9sister \u00e0 des conditions agressives comme des injections de larges volumes d\u2019eau, sans perte de phase stationnaire.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 8 :<\/strong>\u00a0D\u2019excellentes formes de pics et une parfaite stabilit\u00e9 des temps de r\u00e9tention, m\u00eame apr\u00e8s des centaines d\u2019injections d\u2019extraits d\u2019\u00e9pinards, d\u00e9montrent les performances robustes des colonnes Raptor Polar X pour l\u2019analyse des compos\u00e9s polaires.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 9 :<\/strong>\u00a0Les colonnes Raptor Polar X offrent une parfaite reproductibilit\u00e9 de lot \u00e0 lot.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.png\" alt=\"Lot-to-Lot Comparison: Polar Pesticides in Solvent on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0543<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0543\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Colonnes Raptor Polar X : une r\u00e9volution dans l\u2019analyse des compos\u00e9s polaires<\/h2>\n\n\n\n<p>L\u2019analyse des compos\u00e9s polaires s\u2019est jusqu\u2019\u00e0 pr\u00e9sent r\u00e9v\u00e9l\u00e9e comme \u00e9tant un d\u00e9fi pour la qualit\u00e9 des donn\u00e9es et la productivit\u00e9 des laboratoires : signal faible, proc\u00e9dures complexes de pr\u00e9paration d\u2019\u00e9chantillons, faible r\u00e9tention&#8230; Les colonnes Raptor Polar X, gr\u00e2ce \u00e0 leur nouvelle chimie de phase stationnaire permettant les modes HILIC et \u00c9change Ionique sur un seul greffon, simplifient consid\u00e9rablement l\u2019analyse de ces compos\u00e9s complexes et vari\u00e9s. En greffant ce ligand unique sur des particules superficiellement poreuses (SPP), Restek a cr\u00e9\u00e9 une phase qui retient et s\u00e9pare efficacement un large spectre de compos\u00e9s polaires. La synergie de ces caract\u00e9ristiques fournit aux scientifiques de toutes industries un outil polyvalent, puissant et productif.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">R\u00e9f\u00e9rences<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>M. Anastassiades, A.-K. Wachtler, D. I. Kolberg, E. Eichhorn, H. Marks, A. Benkenstein, S. Zechmann; D. Mack, C. Wildgrube, A. Barth, I. Sigalov, S. G\u00f6rlich, D. D\u00f6rk, G. Cerchia, Quick method for the analysis of highly polar pesticides in food involving extraction with acidified methanol and LC &#8211; or ICMS\/MS Measurement &#8211; I. Food of Plant Origin (QuPPe-PO-Method)\u2013Version 12 (published on EURL-SRM website on July 23, 2021).\u00a0<a href=\"https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">                                Catalog No. 32475                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Solution de Passivation LC, Acide m\u00e9thyl\u00e8nediphosphonique (Acide M\u00e9dronique), 1760 \u00b5g\/ml, m\u00e9thanol (qualit\u00e9 HPLC)\/eau (50:50), ampoule de 1 ml<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>La phase stationnaire hybride unique des colonnes Raptor Polar X combine les modes de r\u00e9tention HILIC et Echange Ionique, ce qui la rend id\u00e9ale pour l&#8217;analyse d&#8217;une large gamme de compos\u00e9s polaires, notamment lorsqu\u2019elle est coupl\u00e9e \u00e0 la spectrom\u00e9trie de masse.<\/p>\n","protected":false},"author":11,"featured_media":7419,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2463,2445],"resource-technique":[2318,2354,2362],"ppma_author":[414],"class_list":["post-43835","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-fr","product-library-colonnes-lc","product-library-produits-pour-la-chromatographie-en-phase-liquide","resource-technique-chromatographie-en-phase-liquide","resource-technique-hilic-fr","resource-technique-ms-ms-fr"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"product-library":[{"value":2463,"label":"Colonnes 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