{"id":43904,"date":"2023-03-09T23:00:00","date_gmt":"2023-03-09T23:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/optimized-isomer-separation-of-synthetic-cathinones-by-lc-msms\/"},"modified":"2026-01-03T17:07:32","modified_gmt":"2026-01-03T17:07:32","slug":"optimized-isomer-separation-of-synthetic-cathinones-by-lc-msms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles\/phfa4018\/optimized-isomer-separation-of-synthetic-cathinones-by-lc-msms","title":{"rendered":"Optimized Isomer Separation of Synthetic Cathinones by LC-MS\/MS"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Ensure crucial isomer separations of synthetic cathinones that are not easily separated with conventional C18 columns.<\/li>\n\n\n\n<li>Overcomes the challenge of not being able to fully separate and quantify isobaric isomers by MS\/MS.<\/li>\n\n\n\n<li>Unique stationary phase provides good retention of aromatic compounds, resulting in sharper peak shapes.<\/li>\n<\/ul>\n\n\n\n<p>In the last decade, synthetic cathinones have become widespread in recreational drug use, prompting a need for forensic toxicology laboratories to develop reliable workflows for their identification and quantification. Synthetic cathinones are derivatives of the naturally occurring compound cathinone and are a unique class of psycho-stimulants. They have an aromatic structure and contain structurally similar isomers that are challenging to separate using conventional C18 columns. A reliable analysis that achieves isomer separation is crucial because the pharmacological and toxicological properties of the various isomers differ significantly. Since the respective ortho, meta, and para isomers of methylmethcathinone (MMC) and methylethcathinone (MEC) are isobaric, they are not easily separated and quantified by MS\/MS.<\/p>\n\n\n\n<p>Here, we show the analysis of synthetic cathinone in serum samples on the Raptor Biphenyl core-shell column by LC-MS\/MS analysis. The phase of this Raptor column exhibits strong pi-interactions, making it especially selective to the separation of the differently substituted aromatic rings that make up these isomers. A deuterated internal standard (butylone-d3) was chosen for use due to its availability and cost savings. Using the method conditions specified below, accurate quantitative results and reliable separations were achieved for the synthetic cathinone isomers of MMC and MEC, in serum samples. Figure 1 shows a section of the chromatogram from a spiked serum sample at 100 ng\/mL to illustrate the isomer separations. Figure 2 shows a spiked serum sample at the limit of quantification, 5 ng\/mL.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0A spiked serum sample at 100 ng\/mL shows excellent isomer separation.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0790.png\" alt=\"Methylmethcathinone (MMC) and Methylethcathinone (MEC) Isomer Separation in Serum at 100 ng\/mL Using the Raptor Biphenyl Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0790<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Parent Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1246815-51-9\/2-MMC\" target=\"_blank\" rel=\"noopener\">2-MMC<\/a><\/td><td class=\"oth\">5.75<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1246816-62-5\/3-MMC\" target=\"_blank\" rel=\"noopener\">3-MMC<\/a><\/td><td class=\"oth\">6.15<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1189726-22-4\/4-MMC\" target=\"_blank\" rel=\"noopener\">4-MMC<\/a><\/td><td class=\"oth\">6.70<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Butylone-D3\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1231710-63-6\/Butylone-D3\" target=\"_blank\" rel=\"noopener\">Butylone-D3<\/a><\/td><td class=\"oth\">7.25<\/td><td class=\"oth\">225.1<\/td><td class=\"oth\">176.9<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2705355-07-1\/2-MEC\" target=\"_blank\" rel=\"noopener\">2-MEC<\/a><\/td><td class=\"oth\">7.77<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2493976-59-1\/3-MEC\" target=\"_blank\" rel=\"noopener\">3-MEC<\/a><\/td><td class=\"oth\">8.30<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1266688-86-1\/4-MEC\" target=\"_blank\" rel=\"noopener\">4-MEC<\/a><\/td><td class=\"oth\">8.71<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0790\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water:methanol 95:5, 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>1.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>12.00<\/td><td>0.5<\/td><td>83<\/td><td>17<\/td><\/tr><tr><td>12.01<\/td><td>0.5<\/td><td>2<\/td><td>98<\/td><\/tr><tr><td>20.00<\/td><td>0.5<\/td><td>2<\/td><td>98<\/td><\/tr><tr><td>20.01<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>23.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>SCIEX API 4000 QTRAP MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimazdu LC 20<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>200 \u00b5L of serum was spiked with 10 \u00b5L internal standard (butylone-D3, 1 \u00b5g\/mL) and prepared to a concentration of 100 ng\/mL with a stock containing the 6 synthetic cathinones. A protein precipitation was performed with 200 \u00b5L methanol. Samples were vortexed and centrifuged for 8 minutes at 1625 rpm. 50 \u00b5L of the supernatant was diluted with 150 \u00b5L water and transferred to a vial for analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>An additional equilibration time of 5 minutes was added before each injection to ensure stable retention times. <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0790.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Spike serum sample at the LOQ, 5 ng\/mL.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0789.png\" alt=\"Methylmethcathinone (MMC) and Methylethcathinone (MEC) Isomer Separation in Serum at 5 ng\/mL Using the Raptor Biphenyl Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0789<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Parent Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1246815-51-9\/2-MMC\" target=\"_blank\" rel=\"noopener\">2-MMC<\/a><\/td><td class=\"oth\">5.95<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1246816-62-5\/3-MMC\" target=\"_blank\" rel=\"noopener\">3-MMC<\/a><\/td><td class=\"oth\">6.25<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-MMC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1189726-22-4\/4-MMC\" target=\"_blank\" rel=\"noopener\">4-MMC<\/a><\/td><td class=\"oth\">6.76<\/td><td class=\"oth\">178.1<\/td><td class=\"oth\">145.1<\/td><td class=\"oth\">160.0<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Butylone-D3\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1231710-63-6\/Butylone-D3\" target=\"_blank\" rel=\"noopener\">Butylone-D3<\/a><\/td><td class=\"oth\">7.45<\/td><td class=\"oth\">225.1<\/td><td class=\"oth\">176.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2705355-07-1\/2-MEC\" target=\"_blank\" rel=\"noopener\">2-MEC<\/a><\/td><td class=\"oth\">7.99<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2493976-59-1\/3-MEC\" target=\"_blank\" rel=\"noopener\">3-MEC<\/a><\/td><td class=\"oth\">8.38<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-MEC\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1266688-86-1\/4-MEC\" target=\"_blank\" rel=\"noopener\">4-MEC<\/a><\/td><td class=\"oth\">8.90<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">174.0<\/td><td class=\"oth\">144.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0789\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water:methanol 95:5, 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>1.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>12.00<\/td><td>0.5<\/td><td>83<\/td><td>17<\/td><\/tr><tr><td>12.01<\/td><td>0.5<\/td><td>2<\/td><td>98<\/td><\/tr><tr><td>20.00<\/td><td>0.5<\/td><td>2<\/td><td>98<\/td><\/tr><tr><td>20.01<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>23.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>SCIEX API 4000 QTRAP MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimazdu LC 20<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>200 \u00b5L of serum was spiked with 10 \u00b5L internal standard (butylone-D3, 1 \u00b5g\/mL) and prepared to a concentration of 5 ng\/mL with a stock containing the 6 synthetic cathinones. A protein precipitation was performed with 200 \u00b5L methanol. Samples were vortexed and centrifuged for 8 minutes at 1625 rpm. 50 \u00b5L of the supernatant was diluted with 150 \u00b5L water and transferred to a vial for analysis. <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>The higher concentration internal standard peak is not shown in order to focus on the lower concentration target analytes. <br \/>An additional equilibration time of 5 minutes was added before each injection to ensure stable retention times.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0789.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Acknowledgements:<\/h2>\n\n\n\n<p>Data courtesy of Alexandra Maas, Ph.D., University of Bonn. Thank you to Mrs. Maas for allowing us to use this data.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">References:<\/h2>\n\n\n\n<p>A. Maas, et al., Separation of ortho, meta and para isomers of methylmethcathinone (MMC) and methylethcathinone (MEC) using LC-ESI-MS\/MS: Application to forensic serum samples, J. Chromatogr. B (2017), http:\/\/dx.doi.org\/10.1016\/j.jchromb.2017.01.046<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Related Products<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A12\">                                Catalog No. 9309A12                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonnes Raptor Biphenyl, 2,7\u00b5m 100 x 2.1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col 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                             <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/26543\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25745\">                                Catalog No. 25745                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raccord \u00e0 serrage manuel EXP2 TI-LOK tout-en-un avec ferrule int\u00e9gr\u00e9e, l\u2019unit\u00e9<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                  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