{"id":45288,"date":"2020-10-26T14:30:00","date_gmt":"2020-10-26T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/residual-pesticides-analysis-of-botanical-ingredients-using-gas-chromatography-triple-quadrupole-mass-spectrometry\/"},"modified":"2026-01-28T17:00:44","modified_gmt":"2026-01-28T17:00:44","slug":"residual-pesticides-analysis-of-botanical-ingredients-using-gas-chromatography-triple-quadrupole-mass-spectrometry","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/articles-zh\/fsar3080\/residual-pesticides-analysis-of-botanical-ingredients-using-gas-chromatography-triple-quadrupole-mass-spectrometry","title":{"rendered":"Residual Pesticides Analysis of Botanical Ingredients Using Gas Chromatography Triple Quadrupole Mass Spectrometry"},"content":{"rendered":"\n

Originally published by Shimadzu Corporation in collaboration with Restek Corporation.<\/em><\/p>\n\n\n\n

Introduction<\/h2>\n\n\n\n

The use of dietary supplements is increasing in the United States. These dietary supplements are made from various dried botanicals, which may contain residual pesticides from agricultural practices, and because of that, they have to be monitored to ensure their quality and prevent exposure. The ingredients are dried which requires the standard QuEChERS methods [1] to be modified to overcome this difficulty. Gas chromatography is the best technique to separate multicomponents including coextracted interferences, and because the triple quadruple mass spectrometer (GC-MS\/MS) is highly sensitive this allows analysis of trace level contamination. In this study, we analyzed over 200 compounds simultaneously using a triple quadrupole gas chromatograph mass spectrometer with the modified QuEChERS method.<\/p>\n\n\n\n

Materials and Methods<\/h2>\n\n\n\n

Pesticides standards, internal standards, quality control standards and a QuEChERS kit were obtained from Restek:<\/p>\n\n\n\n

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  • GC Multiresidue Pesticide Kit (cat.# 32562)<\/li>\n\n\n\n
  • QuEChERS Internal Standard Mix for GC-MS Analysis (cat.# 33267)<\/li>\n\n\n\n
  • SV Internal Standard Mix (cat.# 31206)<\/li>\n\n\n\n
  • Q-sep QuEChERS Extraction Kit (Original cat.# 23991 discontinued; see cat.# 25848)<\/li>\n<\/ul>\n\n\n\n

    The total number of targets was 232 compounds (220 pesticides, 6 internal standards, and 6 quality control standards). Ginseng, which can be purchased in any store, was used as a matrix. Using this ginseng, matrix-matched calibration standards (1 to 200 ng\/mL) and fortified samples (each two 10 and 50 ng\/g) were prepared. Calibration curves were generated by an internal standard method, weighted 1\/C and the internal standard was PCB52.<\/p>\n\n\n\n

    An MRM analytical method was created using the Smart Pesticides Database (Shimadzu). This database has retention indices for all registered compounds, and retention times can be predicted by running an n<\/em>-alkane sample mixture (AART: Automatic Adjustment of Retention Time). According to estimated retention times, Smart MRM creates an optimum data acquisition time program (Figure 1).<\/p>\n\n\n

    Figure 1:<\/strong>\u00a0Data Acquisition Time Program Created by Smart MRM<\/div>
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