{"id":51649,"date":"2024-02-28T00:00:00","date_gmt":"2024-02-28T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/column-selection-for-usp-467-using-proezgc\/"},"modified":"2026-01-28T15:51:09","modified_gmt":"2026-01-28T15:51:09","slug":"column-selection-for-usp-467-using-proezgc","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/blog-fr\/gnbl4630\/column-selection-for-usp-467-using-proezgc","title":{"rendered":"Column selection for USP <467> using ProEZ<\/em>GC"},"content":{"rendered":"\n

There are a lot of decisions to be made when performing USP <467>. With three classes of solvents to potentially monitor, two phase materials to choose from, two dimensions for each phase, and the option to select and validate your column of choice, it can be daunting to start thinking about analysis. In addition, unless you are making reference standards in-house, residual solvent stocks are sub-divided into a series of mixtures to be run individually, rather than one \u2018megamix\u2019. This helps improve separations, save time, and make your lab more efficient, because one run with fewer compounds is faster and easier to analyze, but it also means adjusting methodology when it comes time to monitor a new solvent, which can cause a rift in efficiency. This is where Restek\u2019s ProEZ<\/em>GC can help.<\/p>\n\n\n\n

Reference standard selection:<\/h2>\n\n\n\n

The first challenge to overcome is what reference standards are needed. The most up-to-date stock solutions offered at Restek are:<\/p>\n\n\n\n

Table 1: USP <467> reference standards available through Restek, color coordinated with Tables 3 and 5.<\/strong><\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n
Cat.#<\/strong><\/td>\nMix<\/strong><\/td>\n<\/tr>\n
36279<\/a><\/td>\nClass 1<\/td>\n<\/tr>\n
36015<\/a><\/td>\nClass 2 Mix A<\/td>\n<\/tr>\n
36280<\/a><\/td>\nClass 2 Mix B<\/td>\n<\/tr>\n
36273<\/a><\/td>\nClass 2 Mix C<\/td>\n<\/tr>\n
36016<\/a><\/td>\nClass 3 Mix A<\/td>\n<\/tr>\n
36014<\/a><\/td>\nClass 3 Mix B<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n

These mixes represent changes that have been implemented in chapter <467> as recently as 2020. They were also used to build our ProEZ<\/em>GC libraries using the recommended Rxi-624sil MS (G43) and Stabilwax (G16) phases. <\/p>\n\n\n\n

Column selection:<\/h2>\n\n\n\n

USP <467> recommends four possible columns, comprised of two phases: G16 and G43, and two dimensions for each phase. The Restek equivalents for these columns are as follows:<\/p>\n\n\n\n

Table 2: Restek columns available in recommended USP <467> phases and dimensions.<\/strong><\/p>\n\n\n\n

Cat.#<\/strong><\/td>Mix<\/strong><\/td><\/tr>
10624<\/a><\/td>(G16) Stabilwax GC Capillary Column, 30 m, 0.32 mm ID, 0.25 \u00b5m<\/td><\/tr>
10625<\/a><\/td>(G16) Stabilwax GC Capillary Column, 30 m, 0.53 mm ID, 0.25 \u00b5m<\/td><\/tr>
13870<\/a><\/td>(G43) Rxi-624Sil MS GC Capillary Column, 30 m, 0.32 mm ID, 1.80 \u00b5m<\/td><\/tr>
13871<\/a><\/td>(G43) Rxi-624Sil MS GC Capillary Column, 30 m, 0.53 mm ID, 3.00 \u00b5m<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

To start exploring separations on these phases and dimensions, go to ProEZ<\/em>GC<\/a>. The simplest way to get started is to copy\/paste your list of compounds either by name or CAS#, into the appropriate textbox. You can search mix by mix, or search for all mixes at once. For this example, I have searched for all compounds at once:<\/p>\n\n\n\n

The first column recommended is a G16 phase. By going to the stationary phase results dropdown, we see the Rxi-624sil MS is recommended next. So here is where you get to decide how to proceed \u2013 you can move forward with either phase! But, for the sake of this article, I moved forward with the Stabilwax.<\/p>\n\n\n\n

\"The<\/figure>\n\n\n\n

Figure 1: The search window for ProEZ<\/em>GC. Arrows indicate the solve button, and dropdown of column phases with solvent libraries available for modeling.<\/strong><\/p>\n\n\n\n

The default dimensions recommended did not match the USP <467>, and I was curious why not. So, I went into the conditions tab, selected the recommended dimensions, and optimized some separations for maximum column efficiency. I then compared separations between recommended Stabilwax column dimensions, and the default dimensions offered.<\/p>\n\n\n\n

\"ProEZGC<\/figure>\n\n\n\n

Figure 2: ProEZ<\/em>GC conditions tab with default options. Arrows indicate the conditions tab, column dimensions dropdown menu, USP <467> recommended column dimensions, oven refinement and optimization button, and radio button to optimize by efficiency.<\/strong><\/p>\n\n\n\n

When comparing the separations across columns, I primarily looked at separations within mixes. While there were many co-elutions between mixes, those can be easily separated by running just one mix at a time. The separations within mixes offer a more complex challenge. <\/p>\n\n\n\n

Below is a table showing the predicted separations across different Stabilwax (G16) columns, where co-elutions within mixes are boxed in red. Different colored rows represent different mixes, and red-cells highlight all co-elutions determined by ProEZ<\/em>GC. After optimization, the recommended column dimensions, cat.# 10624<\/a> and 10625<\/a>, each had four within-mix co-elutions, while the default recommendation by ProEZGC (cat.# 10654<\/a>) had only two.<\/p>\n\n\n\n

Table 3: Comparison of G16 column dimensions by co-elution. Colors represent different mixes (see Table 1), red represents co-elutions, red-boxes represent within-mix co-elutions.<\/strong><\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
#<\/strong><\/td>\nStabilwax Compound<\/strong><\/td>\nStabilwax <\/strong>cat.# 10624<\/strong><\/a><\/td>\nStabilwax <\/strong>cat.# 10625<\/strong><\/a><\/td>\nStabilwax <\/strong>cat.# 10654<\/strong><\/a><\/td>\n<\/tr>\n
Compound<\/strong><\/td>\nRT<\/strong><\/td>\nRes<\/strong><\/td>\nRT<\/strong><\/td>\nRes<\/strong><\/td>\nRT<\/strong><\/td>\n<\/tr>\n
1<\/td>\nn<\/em>-Pentane<\/td>\n1.74<\/td>\n4<\/td>\n2.36<\/td>\n1.9<\/td>\n1.86<\/td>\n14.4<\/td>\n<\/tr>\n
2<\/td>\nC6<\/td>\n1.82<\/td>\n2.4<\/td>\n2.43<\/td>\n1.2<\/td>\n2.18<\/td>\n7.4<\/td>\n<\/tr>\n
3<\/td>\nDiethyl ether<\/td>\n1.87<\/td>\n2.4<\/td>\n2.47<\/td>\n1.2<\/td>\n2.37<\/td>\n7.4<\/td>\n<\/tr>\n
4<\/td>\nMethyl-tert-butyl ether<\/td>\n1.99<\/td>\n1.2<\/td>\n2.57<\/td>\n0.6<\/td>\n2.84<\/td>\n2.9<\/td>\n<\/tr>\n
5<\/td>\nn<\/em>-Heptane<\/td>\n2.01<\/td>\n1.2<\/td>\n2.59<\/td>\n0.6<\/td>\n2.94<\/td>\n2.9<\/td>\n<\/tr>\n
6<\/td>\nCyclohexane<\/td>\n2.1<\/td>\n2.4<\/td>\n2.66<\/td>\n1.2<\/td>\n3.28<\/td>\n5.3<\/td>\n<\/tr>\n
7<\/td>\n1,1-Dichloroethene<\/td>\n2.15<\/td>\n2.4<\/td>\n2.71<\/td>\n1.2<\/td>\n3.5<\/td>\n5.3<\/td>\n<\/tr>\n
8<\/td>\nMethylcyclohexane<\/td>\n2.31<\/td>\n6.7<\/td>\n2.88<\/td>\n3.5<\/td>\n4.13<\/td>\n13.9<\/td>\n<\/tr>\n
9<\/td>\nAcetone<\/td>\n2.65<\/td>\n4.4<\/td>\n3.12<\/td>\n2.5<\/td>\n5.5<\/td>\n7.1<\/td>\n<\/tr>\n
10<\/td>\nEthyl formate<\/td>\n2.79<\/td>\n0.3<\/td>\n3.23<\/td>\n0.1<\/td>\n6.02<\/td>\n0.4<\/td>\n<\/tr>\n
11<\/td>\nMethyl acetate<\/td>\n2.79<\/td>\n0.3<\/td>\n3.24<\/td>\n0.1<\/td>\n6.06<\/td>\n0.4<\/td>\n<\/tr>\n
12<\/td>\nTetrahydrofuran<\/td>\n3.05<\/td>\n1.3<\/td>\n3.45<\/td>\n0.7<\/td>\n7.03<\/td>\n1.6<\/td>\n<\/tr>\n
13<\/td>\nTriethylamine<\/td>\n3.09<\/td>\n1.3<\/td>\n3.48<\/td>\n0.7<\/td>\n7.17<\/td>\n1.6<\/td>\n<\/tr>\n
14<\/td>\ntrans<\/em>-1,2-Dichloroethene<\/td>\n3.35<\/td>\n0.4<\/td>\n3.7<\/td>\n0.3<\/td>\n7.95<\/td>\n1.2<\/td>\n<\/tr>\n
15<\/td>\n1,1,1-Trichloroethane<\/td>\n3.37<\/td>\n0.1<\/td>\n3.71<\/td>\n0.1<\/td>\n8.05<\/td>\n0.1<\/td>\n<\/tr>\n
16<\/td>\nCarbon tetrachloride<\/td>\n3.38<\/td>\n0.1<\/td>\n3.72<\/td>\n0.1<\/td>\n8.07<\/td>\n0.1<\/td>\n<\/tr>\n
17<\/td>\nEthyl acetate<\/td>\n3.49<\/td>\n2.9<\/td>\n3.81<\/td>\n1.8<\/td>\n8.37<\/td>\n3.7<\/td>\n<\/tr>\n
18<\/td>\nMethanol<\/td>\n3.66<\/td>\n1.9<\/td>\n3.99<\/td>\n1.1<\/td>\n8.79<\/td>\n2.5<\/td>\n<\/tr>\n
19<\/td>\n2-Butanone<\/td>\n3.75<\/td>\n1.9<\/td>\n4.03<\/td>\n1.1<\/td>\n8.98<\/td>\n2.5<\/td>\n<\/tr>\n
20<\/td>\nIsopropyl acetate<\/td>\n4.12<\/td>\n0.1<\/td>\n4.33<\/td>\n—<\/td>\n9.51<\/td>\n3.5<\/td>\n<\/tr>\n
21<\/td>\n1,2-Dimethoxyethane<\/td>\n4.12<\/td>\n0.1<\/td>\n4.34<\/td>\n—<\/td>\n9.75<\/td>\n3.3<\/td>\n<\/tr>\n
22<\/td>\n2-Propanol<\/td>\n4.29<\/td>\n1.3<\/td>\n4.48<\/td>\n0.8<\/td>\n10<\/td>\n1.9<\/td>\n<\/tr>\n
23<\/td>\nMethylene chloride<\/td>\n4.36<\/td>\n1.3<\/td>\n4.54<\/td>\n0.8<\/td>\n10.14<\/td>\n1.9<\/td>\n<\/tr>\n
24<\/td>\nBenzene<\/td>\n4.48<\/td>\n2.2<\/td>\n4.63<\/td>\n1.4<\/td>\n10.39<\/td>\n3.3<\/td>\n<\/tr>\n
25<\/td>\nEthanol<\/td>\n4.81<\/td>\n6.1<\/td>\n4.91<\/td>\n4<\/td>\n10.81<\/td>\n5.7<\/td>\n<\/tr>\n
26<\/td>\nPropyl acetate<\/td>\n5.59<\/td>\n6.3<\/td>\n5.56<\/td>\n4.5<\/td>\n11.82<\/td>\n5.8<\/td>\n<\/tr>\n
27<\/td>\nTrichloroethene<\/td>\n6.02<\/td>\n5.3<\/td>\n5.94<\/td>\n3.7<\/td>\n12.23<\/td>\n5.7<\/td>\n<\/tr>\n
28<\/td>\nAcetonitrile<\/td>\n6.38<\/td>\n1.3<\/td>\n6.27<\/td>\n0.9<\/td>\n12.57<\/td>\n1.5<\/td>\n<\/tr>\n
29<\/td>\n4-Methyl-2-pentanone<\/td>\n6.47<\/td>\n1.3<\/td>\n6.35<\/td>\n0.9<\/td>\n12.65<\/td>\n1.5<\/td>\n<\/tr>\n
30<\/td>\nIsobutyl acetate<\/td>\n6.7<\/td>\n3.3<\/td>\n6.55<\/td>\n2.3<\/td>\n12.77<\/td>\n2.2<\/td>\n<\/tr>\n
31<\/td>\nChloroform<\/td>\n6.94<\/td>\n2.3<\/td>\n6.76<\/td>\n1.7<\/td>\n12.91<\/td>\n0.6<\/td>\n<\/tr>\n
32<\/td>\n2-Butanol<\/td>\n7.1<\/td>\n2.3<\/td>\n6.9<\/td>\n1.7<\/td>\n12.94<\/td>\n0.6<\/td>\n<\/tr>\n
33<\/td>\nToluene<\/td>\n7.34<\/td>\n1.9<\/td>\n7.09<\/td>\n1.5<\/td>\n13.15<\/td>\n4<\/td>\n<\/tr>\n
34<\/td>\n1-Propanol<\/td>\n7.47<\/td>\n1.9<\/td>\n7.2<\/td>\n1.5<\/td>\n13.32<\/td>\n4<\/td>\n<\/tr>\n
35<\/td>\n1,4-Dioxane<\/td>\n7.96<\/td>\n7.3<\/td>\n7.58<\/td>\n5.2<\/td>\n13.66<\/td>\n3.2<\/td>\n<\/tr>\n
36<\/td>\n1,2-Dichloroethane<\/td>\n8.44<\/td>\n—<\/td>\n7.94<\/td>\n—<\/td>\n13.79<\/td>\n—<\/td>\n<\/tr>\n
37<\/td>\nButyl acetate<\/td>\n8.45<\/td>\n—<\/td>\n7.95<\/td>\n—<\/td>\n13.8<\/td>\n—<\/td>\n<\/tr>\n
38<\/td>\n2-Hexanone<\/td>\n8.64<\/td>\n3<\/td>\n8.09<\/td>\n2.1<\/td>\n13.95<\/td>\n2.1<\/td>\n<\/tr>\n
39<\/td>\nIsobutanol<\/td>\n9.12<\/td>\n7.5<\/td>\n8.43<\/td>\n5.4<\/td>\n14.03<\/td>\n2.1<\/td>\n<\/tr>\n
40<\/td>\nEthylbenzene<\/td>\n9.95<\/td>\n3.5<\/td>\n9<\/td>\n2.5<\/td>\n14.65<\/td>\n1.5<\/td>\n<\/tr>\n
41<\/td>\np<\/em>-Xylene<\/td>\n10.17<\/td>\n2.8<\/td>\n9.14<\/td>\n2<\/td>\n14.7<\/td>\n1.5<\/td>\n<\/tr>\n
42<\/td>\nm<\/em>-Xylene<\/td>\n10.35<\/td>\n2.8<\/td>\n9.26<\/td>\n2<\/td>\n14.75<\/td>\n1.5<\/td>\n<\/tr>\n
43<\/td>\n1-Butanol<\/td>\n10.66<\/td>\n4.9<\/td>\n9.45<\/td>\n3.4<\/td>\n14.83<\/td>\n2.2<\/td>\n<\/tr>\n
44<\/td>\nNitromethane<\/td>\n11.01<\/td>\n4.3<\/td>\n9.66<\/td>\n3<\/td>\n14.95<\/td>\n3.5<\/td>\n<\/tr>\n
45<\/td>\nIsopropylbenzene<\/td>\n11.26<\/td>\n2.2<\/td>\n9.81<\/td>\n1.5<\/td>\n15.17<\/td>\n0.1<\/td>\n<\/tr>\n
46<\/td>\nPyridine<\/td>\n11.39<\/td>\n1.5<\/td>\n9.88<\/td>\n0.9<\/td>\n15.17<\/td>\n0.1<\/td>\n<\/tr>\n
47<\/td>\n2-Methoxyethanol<\/td>\n11.48<\/td>\n1.5<\/td>\n9.93<\/td>\n0.9<\/td>\n15.26<\/td>\n2.7<\/td>\n<\/tr>\n
48<\/td>\n3-Methyl-1-butanol<\/td>\n12.19<\/td>\n2<\/td>\n10.31<\/td>\n1.6<\/td>\n15.35<\/td>\n2.8<\/td>\n<\/tr>\n
49<\/td>\nChlorobenzene<\/td>\n12.28<\/td>\n2<\/td>\n10.38<\/td>\n1.6<\/td>\n15.55<\/td>\n2.5<\/td>\n<\/tr>\n
50<\/td>\n2-Ethoxyethanol<\/td>\n12.39<\/td>\n2.5<\/td>\n10.44<\/td>\n1.6<\/td>\n15.63<\/td>\n2.5<\/td>\n<\/tr>\n
51<\/td>\n1-Pentanol<\/td>\n12.9<\/td>\n12.5<\/td>\n10.78<\/td>\n8.7<\/td>\n15.72<\/td>\n3<\/td>\n<\/tr>\n
52<\/td>\nN,N-Dimethylformamide<\/td>\n13.83<\/td>\n4.9<\/td>\n11.46<\/td>\n3.2<\/td>\n16.6<\/td>\n2.4<\/td>\n<\/tr>\n
53<\/td>\nAnisole<\/td>\n13.97<\/td>\n4.9<\/td>\n11.57<\/td>\n3.2<\/td>\n16.67<\/td>\n2.4<\/td>\n<\/tr>\n
54<\/td>\nN,N-Dimethylacetamide<\/td>\n14.49<\/td>\n17.6<\/td>\n11.97<\/td>\n11.8<\/td>\n17.13<\/td>\n5.3<\/td>\n<\/tr>\n
55<\/td>\nAcetic acid<\/td>\n14.95<\/td>\n17.6<\/td>\n12.34<\/td>\n11.5<\/td>\n17.29<\/td>\n5.3<\/td>\n<\/tr>\n
56<\/td>\nTetralin<\/td>\n15.36<\/td>\n9.9<\/td>\n12.66<\/td>\n6.6<\/td>\n17.93<\/td>\n4.1<\/td>\n<\/tr>\n
57<\/td>\nDimethyl sulfoxide<\/td>\n15.6<\/td>\n1.6<\/td>\n12.86<\/td>\n1.5<\/td>\n18.04<\/td>\n4.1<\/td>\n<\/tr>\n
58<\/td>\nFormic acid<\/td>\n15.64<\/td>\n1.6<\/td>\n12.9<\/td>\n1.5<\/td>\n18.22<\/td>\n1.5<\/td>\n<\/tr>\n
59<\/td>\nEthylene glycol<\/td>\n15.82<\/td>\n8.8<\/td>\n13.04<\/td>\n5.4<\/td>\n18.27<\/td>\n1.5<\/td>\n<\/tr>\n
60<\/td>\nN-Methylpyrrolidone<\/td>\n16.16<\/td>\n15.5<\/td>\n13.3<\/td>\n9.5<\/td>\n18.9<\/td>\n9<\/td>\n<\/tr>\n
61<\/td>\nFormamide<\/td>\n16.6<\/td>\n18.5<\/td>\n13.67<\/td>\n12.8<\/td>\n19.17<\/td>\n9<\/td>\n<\/tr>\n
62<\/td>\nSulfolane<\/td>\n16.65<\/td>\n94.8<\/td>\n15.35<\/td>\n62.6<\/td>\n24.18<\/td>\n168<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n

The optimized GC conditions for each column are as follows:<\/p>\n\n\n\n

Table 4: GC Conditions for columns in Table 3.<\/strong><\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Column:<\/td>\nStabilwax, 30.00 m, 0.32 mm ID, 0.25 \u00b5m (cat.# 10624<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 1.79 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n29.48 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 5.5 min) to 70 \u00b0C @ 6.5 \u00b0C\/min to 225 \u00b0C @ 26 \u00b0C\/min (hold 3 min)<\/td>\n<\/tr>\n
Column:<\/td>\nStabilwax, 30.00 m, 0.53 mm ID, 0.25 \u00b5m (cat.# 10625<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 2.97 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n21.48 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 6 min) to 75 \u00b0C @ 13.5 \u00b0C\/min to 225 \u00b0C @ 30 \u00b0C\/min (hold 3 min)<\/td>\n<\/tr>\n
Column:<\/td>\nStabilwax, 30.00 m, 0.32 mm ID, 1.00 \u00b5m (cat.# 10654<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 1.79 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n29.65 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 6.5 min) to 65 \u00b0C @ 6.5 \u00b0C\/min to 225 \u00b0C @ 23 \u00b0C\/min (hold 7 min)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n

I repeated this experiment for the Rxi-624sil MS to preview what separations I would encounter on the alternative set of phases and dimensions. Below is a table showing the predicted separations across different columns, this time using the Rxi-624sil MS phase. Different colored rows represent different mixes, and red-cells highlight all co-elutions determined by ProEZ<\/em>GC. After optimization, the recommended column dimension cat.# 13870<\/a> has two within-mix co-elutions, and cat.# 13871<\/a> had four. The default recommendation by ProEZGC (cat.# 13868<\/a>) also only had two within-mix co-elutions, but provided improved separation of isopropyl acetate and m-xylene relative to cat.# 13870<\/a>.<\/p>\n\n\n\n

Table 5: Comparison of G43 column dimensions by co-elution. Colors represent different mixes (see Table 1), red represents co-elutions, red-boxes represent within-mix co-elutions.<\/strong><\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
#<\/strong><\/td>\nRxi-624Sil MS Compound<\/strong><\/td>\nRxi-624Sil MS <\/strong>cat.# 13870<\/strong><\/a><\/td>\nRxi-624Sil MS <\/strong>cat.# 13871<\/strong><\/a><\/td>\nRxi-624Sil MS <\/strong>cat.# 13868<\/strong><\/a><\/td>\n<\/tr>\n
RT<\/strong><\/td>\nRes<\/strong><\/td>\nRT<\/strong><\/td>\nRes<\/strong><\/td>\nRT<\/strong><\/td>\nRes<\/strong><\/td>\n<\/tr>\n
1<\/td>\nEthylbenzene<\/td>\n2.48<\/td>\n22.9<\/td>\n3.22<\/td>\n15.7<\/td>\n2.39<\/td>\n28<\/td>\n<\/tr>\n
2<\/td>\nAnisole<\/td>\n3.33<\/td>\n4.6<\/td>\n4.33<\/td>\n3.1<\/td>\n3.21<\/td>\n5.5<\/td>\n<\/tr>\n
3<\/td>\np<\/em>-Xylene<\/td>\n3.57<\/td>\n2.4<\/td>\n4.64<\/td>\n1.6<\/td>\n3.44<\/td>\n2.9<\/td>\n<\/tr>\n
4<\/td>\n1,2-Dichloroethane<\/td>\n3.7<\/td>\n2.4<\/td>\n4.81<\/td>\n1.6<\/td>\n3.56<\/td>\n2.9<\/td>\n<\/tr>\n
5<\/td>\nEthylene glycol<\/td>\n4.06<\/td>\n2.1<\/td>\n5.28<\/td>\n1.5<\/td>\n3.92<\/td>\n2.6<\/td>\n<\/tr>\n
6<\/td>\n4-Methyl-2-pentanone<\/td>\n4.2<\/td>\n2.1<\/td>\n5.46<\/td>\n1.5<\/td>\n4.05<\/td>\n2.6<\/td>\n<\/tr>\n
7<\/td>\nIsopropyl acetate<\/td>\n4.52<\/td>\n1.2<\/td>\n5.88<\/td>\n0.8<\/td>\n4.35<\/td>\n1.4<\/td>\n<\/tr>\n
8<\/td>\nm<\/em>-Xylene<\/td>\n4.6<\/td>\n1.2<\/td>\n5.98<\/td>\n0.8<\/td>\n4.43<\/td>\n1.4<\/td>\n<\/tr>\n
9<\/td>\nMethylcyclohexane<\/td>\n4.77<\/td>\n2.3<\/td>\n6.2<\/td>\n1.6<\/td>\n4.59<\/td>\n2.8<\/td>\n<\/tr>\n
10<\/td>\nToluene<\/td>\n4.96<\/td>\n2.4<\/td>\n6.46<\/td>\n1.7<\/td>\n4.78<\/td>\n2.9<\/td>\n<\/tr>\n
11<\/td>\nChlorobenzene<\/td>\n5.15<\/td>\n2.4<\/td>\n6.7<\/td>\n1.7<\/td>\n4.96<\/td>\n2.9<\/td>\n<\/tr>\n
12<\/td>\nPropyl acetate<\/td>\n5.8<\/td>\n0.3<\/td>\n7.47<\/td>\n0.2<\/td>\n5.59<\/td>\n0.4<\/td>\n<\/tr>\n
13<\/td>\nn<\/em>-Pentane<\/td>\n5.84<\/td>\n0.3<\/td>\n7.5<\/td>\n0.2<\/td>\n5.62<\/td>\n0.4<\/td>\n<\/tr>\n
14<\/td>\n2-Methoxyethanol<\/td>\n6.57<\/td>\n8.2<\/td>\n8.06<\/td>\n4.9<\/td>\n6.29<\/td>\n8.4<\/td>\n<\/tr>\n
15<\/td>\nEthyl formate<\/td>\n7.39<\/td>\n6.8<\/td>\n8.55<\/td>\n5.2<\/td>\n6.84<\/td>\n8.4<\/td>\n<\/tr>\n
16<\/td>\nTetrahydrofuran<\/td>\n7.94<\/td>\n2.7<\/td>\n9.01<\/td>\n1.9<\/td>\n7.28<\/td>\n3.2<\/td>\n<\/tr>\n
17<\/td>\nIsobutyl acetate<\/td>\n8.12<\/td>\n1.7<\/td>\n9.17<\/td>\n0.9<\/td>\n7.43<\/td>\n1.8<\/td>\n<\/tr>\n
18<\/td>\nC6<\/td>\n8.22<\/td>\n1.5<\/td>\n9.24<\/td>\n0.8<\/td>\n7.51<\/td>\n1.6<\/td>\n<\/tr>\n
19<\/td>\n1,2-Dimethoxyethane<\/td>\n8.32<\/td>\n1.5<\/td>\n9.31<\/td>\n0.8<\/td>\n7.58<\/td>\n1.6<\/td>\n<\/tr>\n
20<\/td>\n2-Ethoxyethanol<\/td>\n8.52<\/td>\n1.9<\/td>\n9.53<\/td>\n0.6<\/td>\n7.78<\/td>\n1.7<\/td>\n<\/tr>\n
21<\/td>\nCyclohexane<\/td>\n8.66<\/td>\n0.5<\/td>\n9.58<\/td>\n0.6<\/td>\n7.85<\/td>\n0.9<\/td>\n<\/tr>\n
22<\/td>\nPyridine<\/td>\n8.66<\/td>\n0.5<\/td>\n9.64<\/td>\n0.8<\/td>\n7.89<\/td>\n0.9<\/td>\n<\/tr>\n
23<\/td>\nTetralin<\/td>\n8.84<\/td>\n1.4<\/td>\n9.84<\/td>\n1<\/td>\n8.07<\/td>\n1.7<\/td>\n<\/tr>\n
24<\/td>\nTriethylamine<\/td>\n8.94<\/td>\n1.4<\/td>\n9.92<\/td>\n1<\/td>\n8.15<\/td>\n1.7<\/td>\n<\/tr>\n
25<\/td>\n3-Methyl-1-butanol<\/td>\n9.06<\/td>\n2.2<\/td>\n10.02<\/td>\n1.4<\/td>\n8.25<\/td>\n2.5<\/td>\n<\/tr>\n
26<\/td>\nButyl acetate<\/td>\n9.29<\/td>\n0.1<\/td>\n10.22<\/td>\n0.3<\/td>\n8.46<\/td>\n0.2<\/td>\n<\/tr>\n
27<\/td>\n1,4-Dioxane<\/td>\n9.3<\/td>\n0.1<\/td>\n10.24<\/td>\n—<\/td>\n8.47<\/td>\n0.2<\/td>\n<\/tr>\n
28<\/td>\nSulfolane<\/td>\n9.33<\/td>\n0.4<\/td>\n10.24<\/td>\n—<\/td>\n8.49<\/td>\n0.3<\/td>\n<\/tr>\n
29<\/td>\nN,N-Dimethylacetamide<\/td>\n9.35<\/td>\n0.3<\/td>\n10.25<\/td>\n—<\/td>\n8.5<\/td>\n0.3<\/td>\n<\/tr>\n
30<\/td>\nEthyl acetate<\/td>\n9.37<\/td>\n0.3<\/td>\n10.3<\/td>\n0.7<\/td>\n8.53<\/td>\n0.7<\/td>\n<\/tr>\n
31<\/td>\nn<\/em>-Heptane<\/td>\n9.46<\/td>\n1.7<\/td>\n10.36<\/td>\n0.7<\/td>\n8.61<\/td>\n1.9<\/td>\n<\/tr>\n
32<\/td>\ncis<\/em>-1,2-Dicholoroethene<\/td>\n9.54<\/td>\n1.7<\/td>\n10.46<\/td>\n1.5<\/td>\n8.69<\/td>\n2.2<\/td>\n<\/tr>\n
33<\/td>\ntrans<\/em>-1,2-Dichloroethene<\/td>\n9.7<\/td>\n3.5<\/td>\n10.61<\/td>\n2.2<\/td>\n8.84<\/td>\n3.9<\/td>\n<\/tr>\n
34<\/td>\nMethyl-tert-butyl ether<\/td>\n10.02<\/td>\n0.4<\/td>\n10.9<\/td>\n0.9<\/td>\n9.13<\/td>\n0.9<\/td>\n<\/tr>\n
35<\/td>\nCarbon tetrachloride<\/td>\n10.04<\/td>\n0.4<\/td>\n10.96<\/td>\n0.9<\/td>\n9.16<\/td>\n0.9<\/td>\n<\/tr>\n
36<\/td>\n2-Hexanone<\/td>\n10.28<\/td>\n2.1<\/td>\n11.21<\/td>\n1.1<\/td>\n9.39<\/td>\n2.3<\/td>\n<\/tr>\n
37<\/td>\nDiethyl ether<\/td>\n10.37<\/td>\n1.8<\/td>\n11.29<\/td>\n0.8<\/td>\n9.47<\/td>\n1.9<\/td>\n<\/tr>\n
38<\/td>\nEthanol<\/td>\n10.45<\/td>\n1.8<\/td>\n11.34<\/td>\n0.8<\/td>\n9.54<\/td>\n1.9<\/td>\n<\/tr>\n
39<\/td>\nMethanol<\/td>\n10.68<\/td>\n5.9<\/td>\n11.6<\/td>\n4.1<\/td>\n9.77<\/td>\n7<\/td>\n<\/tr>\n
40<\/td>\n2-Propanol<\/td>\n11.05<\/td>\n0.7<\/td>\n11.98<\/td>\n0.8<\/td>\n10.13<\/td>\n1<\/td>\n<\/tr>\n
41<\/td>\nAcetone<\/td>\n11.08<\/td>\n0.7<\/td>\n12.03<\/td>\n0.4<\/td>\n10.17<\/td>\n1<\/td>\n<\/tr>\n
42<\/td>\nChloroform<\/td>\n11.14<\/td>\n1.5<\/td>\n12.06<\/td>\n0.4<\/td>\n10.22<\/td>\n1.5<\/td>\n<\/tr>\n
43<\/td>\n1-Propanol<\/td>\n11.22<\/td>\n0.6<\/td>\n12.16<\/td>\n0.3<\/td>\n10.31<\/td>\n0.4<\/td>\n<\/tr>\n
44<\/td>\n1-Butanol<\/td>\n11.25<\/td>\n0.6<\/td>\n12.19<\/td>\n0.3<\/td>\n10.32<\/td>\n0.4<\/td>\n<\/tr>\n
45<\/td>\n1-Pentanol<\/td>\n11.34<\/td>\n2.5<\/td>\n12.28<\/td>\n1.5<\/td>\n10.42<\/td>\n3.1<\/td>\n<\/tr>\n
46<\/td>\nBenzene<\/td>\n11.55<\/td>\n5.4<\/td>\n12.5<\/td>\n3.6<\/td>\n10.62<\/td>\n6.5<\/td>\n<\/tr>\n
47<\/td>\n1,1,1-Trichloroethane<\/td>\n11.75<\/td>\n2.6<\/td>\n12.72<\/td>\n1.6<\/td>\n10.83<\/td>\n3.1<\/td>\n<\/tr>\n
48<\/td>\nAcetonitrile<\/td>\n11.85<\/td>\n2.6<\/td>\n12.82<\/td>\n1.6<\/td>\n10.92<\/td>\n3.1<\/td>\n<\/tr>\n
49<\/td>\nMethylene chloride<\/td>\n12<\/td>\n4.1<\/td>\n12.98<\/td>\n2.5<\/td>\n11.07<\/td>\n4.9<\/td>\n<\/tr>\n
50<\/td>\nFormamide<\/td>\n12.39<\/td>\n1.8<\/td>\n13.44<\/td>\n1.1<\/td>\n11.47<\/td>\n2.1<\/td>\n<\/tr>\n
51<\/td>\n1,1-Dichloroethene<\/td>\n12.46<\/td>\n1.8<\/td>\n13.51<\/td>\n1.1<\/td>\n11.55<\/td>\n2.1<\/td>\n<\/tr>\n
52<\/td>\nNitromethane<\/td>\n12.56<\/td>\n—<\/td>\n13.62<\/td>\n—<\/td>\n11.65<\/td>\n—<\/td>\n<\/tr>\n
53<\/td>\nIsobutanol<\/td>\n12.56<\/td>\n—<\/td>\n13.62<\/td>\n—<\/td>\n11.65<\/td>\n—<\/td>\n<\/tr>\n
54<\/td>\n2-Butanol<\/td>\n13.07<\/td>\n1.8<\/td>\n14.18<\/td>\n1.3<\/td>\n12.17<\/td>\n2.2<\/td>\n<\/tr>\n
55<\/td>\n2-Butanone<\/td>\n13.14<\/td>\n1.7<\/td>\n14.26<\/td>\n1.1<\/td>\n12.24<\/td>\n2<\/td>\n<\/tr>\n
56<\/td>\nTrichloroethene<\/td>\n13.2<\/td>\n1.7<\/td>\n14.33<\/td>\n\n

 <\/p>\n

1.1<\/p>\n<\/td>\n

12.3<\/td>\n2<\/td>\n<\/tr>\n
57<\/td>\nMethyl acetate<\/td>\n14.81<\/td>\n18.4<\/td>\n16.14<\/td>\n12.6<\/td>\n13.96<\/td>\n22.3<\/td>\n<\/tr>\n
58<\/td>\nN-Methylpyrrolidone<\/td>\n15.49<\/td>\n18.4<\/td>\n16.92<\/td>\n12.6<\/td>\n14.66<\/td>\n22.3<\/td>\n<\/tr>\n
59<\/td>\nIsopropylbenzene<\/td>\n16.59<\/td>\n28.9<\/td>\n18.17<\/td>\n19.7<\/td>\n15.8<\/td>\n34.6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n

The optimized GC conditions for each column are as follows:<\/p>\n\n\n\n

Table 6: GC Conditions for columns in Table 5.<\/strong><\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Column:<\/td>\nStabilwax, 30.00 m, 0.32 mm ID, 0.25 \u00b5m (cat.# 10624<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 1.79 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n29.48 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 5.5 min) to 70 \u00b0C @ 6.5 \u00b0C\/min to 225 \u00b0C @ 26 \u00b0C\/min (hold 3 min)<\/td>\n<\/tr>\n
Column:<\/td>\nStabilwax, 30.00 m, 0.53 mm ID, 0.25 \u00b5m (cat.# 10625<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 2.97 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n21.48 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 6 min) to 75 \u00b0C @ 13.5 \u00b0C\/min to 225 \u00b0C @ 30 \u00b0C\/min (hold 3 min)<\/td>\n<\/tr>\n
Column:<\/td>\nStabilwax, 30.00 m, 0.32 mm ID, 1.00 \u00b5m (cat.# 10654<\/a>)<\/td>\n<\/tr>\n
Carrier Gas:<\/td>\nHelium, Constant Flow @ 1.79 mL\/min<\/td>\n<\/tr>\n
Average Velocity:<\/td>\n29.65 cm\/sec<\/td>\n<\/tr>\n
Outlet Pressure (abs):<\/td>\n14.70 psi<\/td>\n<\/tr>\n
Oven Temp.:<\/td>\n30 \u00b0C (hold 6.5 min) to 65 \u00b0C @ 6.5 \u00b0C\/min to 225 \u00b0C @ 23 \u00b0C\/min (hold 7 min)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n

Overall, this comparison provided some important insight into the separations to expect when changing column dimensions. The ProEZ<\/em>GC default dimensions certainly seemed to provide better solutions then the USP <467> recommended dimensions for both phases. Depending on your analysis, this might save you a significant amount of trial-and-error trying to determine which of the suggested columns will work best.<\/p>\n\n\n\n

If we choose to compare instead between the phases, using results from the ProEZ<\/em>GC recommended phases, we see that each column results in two within-mix co-elutions. Using the Stabilwax, we can expect ethyl formate and methyl acetate from Class 3 Mix A, and 1,1,1-Trichloroethane and carbon tetrachloride from Class 1 mix, to co-elute. Using the Rxi-624sil MS, we can expect isopropyl acetate and m-xylene from Class 3 Mix A, and nitromethane and isobutanol from Class 2 Mix A, to co-elute. All other co-elutions can be overcome by running one mix at a time. Depending on which solvents are more relevant to your lab, either column may be ideal.<\/p>\n\n\n\n

Conclusions:<\/h2>\n\n\n\n

With so many options to sift through, and so many opportunities to tailor your lab efficiency, implementing USP <467> can be complicated. With the help of ProEZ<\/em>GC, Restek can helps your lab get running as fast as possible, and with as little troubleshooting as possible, as you build GC methods that match your labs needs.<\/p>\n\n\n\n

<\/div>\n\n\n
\n

Products Mentioned<\/h3>\n
\n
\n
\n
\n Catalog No. 36279 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels – Classe 1, Dans le dim\u00e9thylsulfoxyde, 1 ml\/ampoule<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 36015 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels de Classe 2 – M\u00e9lange A (r\u00e9vision 2020), 0.35-22.5 mg\/mL dans le DMSO (dimethylsulfoxide), 1 mL\/ampoule<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 36280 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels, Classe 2 – M\u00e9lange B, DMSO, 1 ml\/ampoule<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 36273 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels – Classe 2 – M\u00e9lange C, Dans le dim\u00e9thylsulfoxyde, 1 ml\/ampoule<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 36016 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels \u2013 Classe 3 \u2013 M\u00e9lange A (r\u00e9vision 2020), 5 000 \u00b5g\/ml de chaque compos\u00e9 dans le N,N-dim\u00e9thylformamide, 1 ml\/ampoule<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 36014 <\/a> <\/div>\n
\n
Solvants r\u00e9siduels de Classe 3 – Solution \u00e9talon B, 5000\u00a0\u00b5g\/ml de chaque compos\u00e9, N,N-dim\u00e9thylformamide, ampoule de 1\u00a0ml<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 10624 <\/a> <\/div>\n
\n
Colonne capillaire Stabilwax, L 30\u00a0m, DI 0.32\u00a0mm, 0.25 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 10625 <\/a> <\/div>\n
\n
Colonne capillaire Stabilwax, L 30\u00a0m, DI 0.53\u00a0mm, 0.25 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 10654 <\/a> <\/div>\n
\n
Colonne capillaire Stabilwax, L 30\u00a0m, DI 0.32\u00a0mm, 1.00 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13870 <\/a> <\/div>\n
\n
Colonne capillaire Rxi-624Sil MS, L 30\u00a0m, DI 0.32\u00a0mm, 1.80 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13871 <\/a> <\/div>\n
\n
Colonne capillaire Rxi-624Sil MS, L 30\u00a0m, DI 0.53\u00a0mm, 3.00 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13868 <\/a> <\/div>\n
\n
Colonne capillaire Rxi-624Sil MS, L 30\u00a0m, DI 0.25\u00a0mm, 1.40 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

There are a lot of decisions to be made when performing USP <467>. With three classes of solvents to potentially monitor, two phase materials to choose from, two dimensions for each phase, and the option to select and validate your column of choice, it can be daunting to start thinking about analysis. This is where Restek’s Pro EZGC software can help.<\/p>\n","protected":false},"author":34,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[791],"tags":[],"industries-application":[2219],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2316],"ppma_author":[454],"class_list":["post-51649","post","type-post","status-publish","format-standard","hentry","category-blog-fr","industries-application-pharmaceutique-biopharmaceutique","resource-technique-chromatographie-en-phase-gazeuse-gc"],"acf":[],"taxonomy_info":{"category":[{"value":791,"label":"Blog"}],"industries-application":[{"value":2219,"label":"Pharmaceutique et biopharmaceutique"}],"resource-technique":[{"value":2316,"label":"Chromatographie en phase gazeuse (GC)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Erica Pack, PhD","author_link":"https:\/\/discover.restek.com\/fr\/author\/erica-pack-phd\/"},"comment_info":0,"category_info":[{"term_id":791,"name":"Blog","slug":"blog-fr","term_group":0,"term_taxonomy_id":791,"taxonomy":"category","description":"","parent":0,"count":444,"filter":"raw","cat_ID":791,"category_count":444,"category_description":"","cat_name":"Blog","category_nicename":"blog-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":454,"user_id":34,"is_guest":0,"slug":"erica-pack-phd","display_name":"Erica Pack, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-pack-erica.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-pack-erica.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/51649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/34"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=51649"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/51649\/revisions"}],"predecessor-version":[{"id":85265,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/51649\/revisions\/85265"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=51649"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=51649"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=51649"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=51649"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=51649"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=51649"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=51649"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=51649"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=51649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}