{"id":51844,"date":"2025-03-24T00:00:00","date_gmt":"2025-03-24T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/using-the-ezlc-modeler-for-cannabinoid-separations-part-4\/"},"modified":"2026-01-28T16:02:58","modified_gmt":"2026-01-28T16:02:58","slug":"using-the-ezlc-modeler-for-cannabinoid-separations-part-4","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/blogs\/gnbl4851\/using-the-ezlc-modeler-for-cannabinoid-separations-part-4","title":{"rendered":"Using the <em>EZ<\/em>LC Modeler for Cannabinoid Separations\u2013Part 4: Using <em>EZ<\/em>LC Software to Harness the Full Separation Capability of Your Column"},"content":{"rendered":"\n<p><style>     ul li { margin-top: 5px; }     ol li { margin-top: 5px; }  <\/style><\/p>\n\n\n\n<p>If you read part three, <a href=\"https:\/\/discover.restek.com\/GNBL4850\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3\/\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/GNBL4850\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3\/\">What You\u2019re Not Monitoring for Still Matters<\/a>, of this four-part blog series, it discussed why it is important to look at additional analytes during method development to ensure there will not be any coelution with analytes that may be in your sample. In this final blog, focus will be placed on utilizing a column to its fullest potential.<\/p>\n\n\n\n<p>It is critical that your current column is capable of resolving all analytes in your current panel. However, it is also important to know if new analytes can be added later using the same column, without sacrificing time, or consumables.<\/p>\n\n\n\n<p>To demonstrate the ability of a column to separate analytes, varying column dimensions were used to model a set of 27 cannabinoids with one set of experimental conditions.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Peak #<\/strong><\/td><td><strong>Analyte<\/strong><\/td><td><strong>Synonym<\/strong><\/td><\/tr><tr><td>1<\/td><td>Cannabidiorcin<\/td><td>CBDO<\/td><\/tr><tr><td>2<\/td><td>Cannabidiethanol<\/td><td>CBDE<\/td><\/tr><tr><td>3<\/td><td>Cannabidivarinic acid<\/td><td>CBDVA<\/td><\/tr><tr><td>4<\/td><td>Cannabigerovarinic acid<\/td><td>CBGVA<\/td><\/tr><tr><td>5<\/td><td>Cannabidibutolic acid<\/td><td>CBDA<\/td><\/tr><tr><td>6<\/td><td>Cannabidibutol<\/td><td>CBDB<\/td><\/tr><tr><td>7<\/td><td>Cannabidiolic acid<\/td><td>CBDA<\/td><\/tr><tr><td>8<\/td><td>Cannabigerolic acid<\/td><td>CBGA<\/td><\/tr><tr><td>9<\/td><td>Cannabigerol<\/td><td>CBG<\/td><\/tr><tr><td>10<\/td><td>Cannabidiol<\/td><td>CBD<\/td><\/tr><tr><td>11<\/td><td>Tetrahydrocannabivarin<\/td><td>THCV<\/td><\/tr><tr><td>12<\/td><td>Cannabigerohexol<\/td><td>CBGH<\/td><\/tr><tr><td>13<\/td><td>Cannabichromevarin<\/td><td>CBCV<\/td><\/tr><tr><td>14<\/td><td>Tetrahydrocannabivarinic acid<\/td><td>THCVA<\/td><\/tr><tr><td>15<\/td><td>Cannabinol<\/td><td>CBN<\/td><\/tr><tr><td>16<\/td><td>Cannabigerophorol<\/td><td>CBGP<\/td><\/tr><tr><td>17<\/td><td>Cannabinolic acid<\/td><td>CBNA<\/td><\/tr><tr><td>18<\/td><td>\u03949-Tetrahydrocannabinol<\/td><td>\u03949-THC<\/td><\/tr><tr><td>19<\/td><td>\u03948-Tetrahydrocannabinol<\/td><td>\u03948-THC<\/td><\/tr><tr><td>20<\/td><td>(6aR,9S)-\u039410-Tetrahydrocannabinol<\/td><td>(6aR,9S)-\u039410-THC<\/td><\/tr><tr><td>21<\/td><td>9(R)-\u03946a,10a Tetrahydrocannabinol<\/td><td>9R-\u03946a,10a &#8211; THC<\/td><\/tr><tr><td>22<\/td><td>Cannabichromene<\/td><td>CBC<\/td><\/tr><tr><td>23<\/td><td>Tetrahydrocannabinolic acid A<\/td><td>THCA-A<\/td><\/tr><tr><td>24<\/td><td>Cannabichromenic acid<\/td><td>CBCA<\/td><\/tr><tr><td>25<\/td><td>Cannabicyclolic acid<\/td><td>CBLA<\/td><\/tr><tr><td>26<\/td><td>Cannabidiorcin<\/td><td>CBDO<\/td><\/tr><tr><td>27<\/td><td>Cannabidiethanol<\/td><td>CBDE<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td colspan=\"3\"><strong>Conditions<\/strong><\/td><\/tr><tr><td>Analytical Column:<\/td><td colspan=\"2\">Raptor ARC-18, 2.7 \u00b5m (varying dimensions)<\/td><\/tr><tr><td>Flow (mL\/min):<\/td><td colspan=\"2\">1<\/td><\/tr><tr><td>Temperature (\u00b0C)<\/td><td colspan=\"2\">30<\/td><\/tr><tr><td>% Mobile Phase A:<\/td><td colspan=\"2\">Water, 3 mM ammonium formate, 0.1 % formic acid<\/td><\/tr><tr><td>% Mobile Phase B:<\/td><td colspan=\"2\">Acetonitrile, 0.1 % formic acid<\/td><\/tr><tr><td>Gradient:<\/td><td colspan=\"2\">Isocratic 26:74<\/td><\/tr><tr><td rowspan=\"3\">Cycle:<\/td><td>Time (min)<\/td><td>(%) B<\/td><\/tr><tr><td>0.00<\/td><td>74<\/td><\/tr><tr><td>10.00<\/td><td>74<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>&nbsp;<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Modeled chromatogram of 27 cannabinoids using 150 x 2.1 mm ID column dimension<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-4-using-EZLC-cannabinoid-separations-01.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>&nbsp;By using a 150 x 2.1 mm column, the modeler indicates that a shortened run time can be achieved, however, full resolution of analytes CBDB and CBDA cannot be achieved. Altering the method parameters causes additional coelution of analytes. Additionally, this column often has a higher pressure profile due to its dimensions along with smaller peak volumes leading to the need for a UHPLC for analysis.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Modeled chromatogram of 27 cannabinoids using 150 x 4.6 mm ID column dimension<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-4-using-EZLC-cannabinoid-separations-02.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>&nbsp;In Figure 2, using a 150 x 4.6 mm column allows for full separation of analytes, but with the caveat of a substantial increase in total run time. However, the run time can be reduced by adjusting the flow to 2.5 mL\/min, giving a full cycle time of approximately nine minutes assuming the instrument can support the increase in backpressure.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0706.png\" alt=\"Column Separation Capability: 27 Cannabinoids on Raptor ARC-18 (2.7 \u03bcm, 150 mm x 3.0 mm ID)\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0706<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Experimental t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Modeled t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Difference (sec)<\/th><th style=\"text-align: center;width: 75px\">Analytical Run Time Difference (%)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiorcin (CBDO)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/35482-50-9\/Cannabidiorcin\" target=\"_blank\" rel=\"noopener\">Cannabidiorcin (CBDO)<\/a><\/td><td class=\"oth\">1.06<\/td><td class=\"oth\">1.06<\/td><td class=\"oth\">0.36<\/td><td class=\"oth\">0.07<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiethanol (CBDE)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2552798-14-6\/Cannabidiethanol\" target=\"_blank\" rel=\"noopener\">Cannabidiethanol (CBDE)<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">1.20<\/td><td class=\"oth\">4.56<\/td><td class=\"oth\">0.84<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidivarinic acid (CBDVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31932-13-5\/Cannabidivarinic acid\" target=\"_blank\" rel=\"noopener\">Cannabidivarinic acid (CBDVA)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">1.28<\/td><td class=\"oth\">6.12<\/td><td class=\"oth\">1.13<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerovarinic acid (CBGVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/64924-07-8\/Cannabigerovarinic acid\" target=\"_blank\" rel=\"noopener\">Cannabigerovarinic acid (CBGVA)<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">1.37<\/td><td class=\"oth\">6.90<\/td><td class=\"oth\">1.28<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidibutolic acid (CBDBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2413020-50-3\/Cannabidibutolic acid\" target=\"_blank\" rel=\"noopener\">Cannabidibutolic acid (CBDBA)<\/a><\/td><td class=\"oth\">1.71<\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">12.96<\/td><td class=\"oth\">2.40<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidibutol (CBDB)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60113-11-3\/Cannabidibutol\" target=\"_blank\" rel=\"noopener\">Cannabidibutol (CBDB)<\/a><\/td><td class=\"oth\">1.80<\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">4.32<\/td><td class=\"oth\">0.80<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiolic acid (CBDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1244-58-2\/Cannabidiolic acid\" target=\"_blank\" rel=\"noopener\">Cannabidiolic acid (CBDA)<\/a><\/td><td class=\"oth\">1.94<\/td><td class=\"oth\">1.81<\/td><td class=\"oth\">7.62<\/td><td class=\"oth\">1.41<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerolic acid (CBGA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25555-57-1\/Cannabigerolic acid\" target=\"_blank\" rel=\"noopener\">Cannabigerolic acid (CBGA)<\/a><\/td><td class=\"oth\">2.04<\/td><td class=\"oth\">1.94<\/td><td class=\"oth\">6.06<\/td><td class=\"oth\">1.12<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerol (CBG)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25654-31-3\/Cannabigerol\" target=\"_blank\" rel=\"noopener\">Cannabigerol (CBG)<\/a><\/td><td class=\"oth\">2.14<\/td><td class=\"oth\">2.05<\/td><td class=\"oth\">5.64<\/td><td class=\"oth\">1.04<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiol (CBD)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13956-29-1\/Cannabidiol\" target=\"_blank\" rel=\"noopener\">Cannabidiol (CBD)<\/a><\/td><td class=\"oth\">2.33<\/td><td class=\"oth\">2.17<\/td><td class=\"oth\">9.48<\/td><td class=\"oth\">1.76<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarin (THCV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31262-37-0\/Tetrahydrocannabivarin\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarin (THCV)<\/a><\/td><td class=\"oth\">2.60<\/td><td class=\"oth\">2.36<\/td><td class=\"oth\">14.70<\/td><td class=\"oth\">2.72<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerohexol (CBGH)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2871775-07-2\/Cannabigerohexol\" target=\"_blank\" rel=\"noopener\">Cannabigerohexol (CBGH)<\/a><\/td><td class=\"oth\">2.86<\/td><td class=\"oth\">2.69<\/td><td class=\"oth\">10.26<\/td><td class=\"oth\">1.90<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromevarin (CBCV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/41408-19-9\/Cannabichromevarin\" target=\"_blank\" rel=\"noopener\">Cannabichromevarin (CBCV)<\/a><\/td><td class=\"oth\">2.99<\/td><td class=\"oth\">2.89<\/td><td class=\"oth\">5.88<\/td><td class=\"oth\">1.09<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Experimental t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Modeled t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Difference (sec)<\/th><th style=\"text-align: center;width: 75px\">Analytical Run Time Difference (%)<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarinic acid (THCVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/39986-26-0\/Tetrahydrocannabivarinic acid\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarinic acid (THCVA)<\/a><\/td><td class=\"oth\">3.24<\/td><td class=\"oth\">3.02<\/td><td class=\"oth\">13.20<\/td><td class=\"oth\">2.44<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinol (CBN)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/521-35-7\/Cannabinol\" target=\"_blank\" rel=\"noopener\">Cannabinol (CBN)<\/a><\/td><td class=\"oth\">3.44<\/td><td class=\"oth\">3.27<\/td><td class=\"oth\">9.84<\/td><td class=\"oth\">1.82<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerophorol (CBGP)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55824-15-2\/Cannabigerophorol\" target=\"_blank\" rel=\"noopener\">Cannabigerophorol (CBGP)<\/a><\/td><td class=\"oth\">3.69<\/td><td class=\"oth\">3.46<\/td><td class=\"oth\">13.74<\/td><td class=\"oth\">2.54<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinolic acid (CBNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2808-39-1\/Cannabinolic acid\" target=\"_blank\" rel=\"noopener\">Cannabinolic acid (CBNA)<\/a><\/td><td class=\"oth\">3.96<\/td><td class=\"oth\">4.01<\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">0.50<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03949-Tetrahydrocannabinol (\u03949-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1972-08-3\/\u03949-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03949-Tetrahydrocannabinol (\u03949-THC)<\/a><\/td><td class=\"oth\">4.10<\/td><td class=\"oth\">4.16<\/td><td class=\"oth\">3.24<\/td><td class=\"oth\">0.60<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03948-Tetrahydrocannabinol (\u03948-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5957-75-5\/\u03948-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03948-Tetrahydrocannabinol (\u03948-THC)<\/a><\/td><td class=\"oth\">4.24<\/td><td class=\"oth\">4.32<\/td><td class=\"oth\">4.86<\/td><td class=\"oth\">0.90<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for (6aR,9S)-\u039410-Tetrahydrocannabinol ((6aR,9S)-\u220610-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/95588-87-7\/(6aR,9S)-\u039410-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">(6aR,9S)-\u039410-Tetrahydrocannabinol ((6aR,9S)-\u220610-THC)<\/a><\/td><td class=\"oth\">4.80<\/td><td class=\"oth\">4.84<\/td><td class=\"oth\">2.46<\/td><td class=\"oth\">0.46<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\">9(R)-\u22066a,10a Tetrahydrocannabinol  (9R-\u22066a,10a &#8211; THC)<\/td><td class=\"oth\">5.01<\/td><td class=\"oth\">5.06<\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">0.50<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromene (CBC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20675-51-8\/Cannabichromene\" target=\"_blank\" rel=\"noopener\">Cannabichromene (CBC)<\/a><\/td><td class=\"oth\">5.19<\/td><td class=\"oth\">5.28<\/td><td class=\"oth\">5.28<\/td><td class=\"oth\">0.98<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\">Tetrahydrocannabinolic acid A (THCA-A) <\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">5.52<\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">0.67<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromenic acid (CBCA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/185505-15-1\/Cannabichromenic acid\" target=\"_blank\" rel=\"noopener\">Cannabichromenic acid (CBCA)<\/a><\/td><td class=\"oth\">6.20<\/td><td class=\"oth\">6.28<\/td><td class=\"oth\">5.04<\/td><td class=\"oth\">0.93<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabicyclolic acid (CBLA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/40524-99-0\/Cannabicyclolic acid\" target=\"_blank\" rel=\"noopener\">Cannabicyclolic acid (CBLA)<\/a><\/td><td class=\"oth\">6.47<\/td><td class=\"oth\">6.54<\/td><td class=\"oth\">4.02<\/td><td class=\"oth\">0.74<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\">\u22069-Tetrahydrocannabiphorol (\u22069-THCP)<\/td><td class=\"oth\">7.96<\/td><td class=\"oth\">8.11<\/td><td class=\"oth\">8.94<\/td><td class=\"oth\">1.66<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabicitran (CBT)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31508-71-1\/Cannabicitran\" target=\"_blank\" rel=\"noopener\">Cannabicitran (CBT)<\/a><\/td><td class=\"oth\">8.22<\/td><td class=\"oth\">8.42<\/td><td class=\"oth\">12.48<\/td><td class=\"oth\">2.31<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A6E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0706\" rel=\"noopener\">cat.# 9314A6E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Cannabinoids acids 7 standard, 1000 \u00b5g\/mL, acetonitrile with 1% DIPEA and 0.05% ascorbic acid (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34144?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0706\" rel=\"noopener\">cat.# 34144<\/a>)<\/td><\/tr><tr><td><\/td><td>Cannabinoids neutrals 9 standard, 1000 \u00b5g\/mL, P&amp;T methanol, 1 mL\/ampul (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34132?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0706\" rel=\"noopener\">cat.# 34132<\/a>)<\/td><\/tr><tr><td><\/td><td>All other cannabinoids were obtained separately. <\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ppm<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 3 mM ammonium formate, 0.1 % formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.1 % formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.00<\/td><td>26<\/td><td>74<\/td><\/tr><tr><td>9.00<\/td><td>1.00<\/td><td>26<\/td><td>74<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 228 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Cell Size:<\/th><td>500 nL <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Working standard was prepared in a 2 mL, 9 mm amber vial (cat. 21142) by diluting 50 \u00b5L of each standard into 900 \u00b5L acetonitrile and capped with a 9 mm short screw cap (cat. 24497).<br \/><\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0706.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p>&nbsp;Finally, testing a 150 x 3.0 mm column dimension not only allows for full chromatographic separation, but also conserves analysis time as well as solvent consumption. By utilizing <em>EZ<\/em>LC software, it is possible to determine if an existing column is capable of adding additional analytes with the current methodology, or if modifications need to be made, or a new column needs to be purchased.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Check Out the Full Blog Series in the Related Resources below.<\/strong><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The final in a four-part series, this blog explores how to use <em>EZ<\/em>LC chromatogram modeling software to determine if new cannabinoids can be added to your current method and, if so, what modifications may be needed for optimal results.<\/p>\n","protected":false},"author":39,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2281,2243],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2334],"hf_cat_post":[623],"ppma_author":[459],"class_list":["post-51844","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-cannabis-it","industries-application-cannabis-terapeutica-ricreativa","resource-technique-cromatografia-liquida-lc"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2281,"label":"Cannabis"},{"value":2243,"label":"Cannabis 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