{"id":60341,"date":"2025-11-05T19:12:20","date_gmt":"2025-11-05T19:12:20","guid":{"rendered":"https:\/\/discover.restek.com\/?p=60341"},"modified":"2026-01-08T14:21:26","modified_gmt":"2026-01-08T14:21:26","slug":"comparing-c18-type-stationary-phases-to-biphenyl-using-an-lc-virtual-method-development-tool","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/posters-presentations\/gnot5298\/comparing-c18-type-stationary-phases-to-biphenyl-using-an-lc-virtual-method-development-tool","title":{"rendered":"Comparing C18-Type Stationary Phases to Biphenyl Using an LC Virtual Method Development Tool"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">As LC-MS\/MS continues to grow as the dominant separation technique within a host of industries, more laboratories are trying to run broad panels of analytes from single injections, requiring robust chromatography capable of separating analytes, isobars, and matrix elements suitably to afford suitable detector sensitivity for qualification and quantitation. Column selection is the single most impactful factor when it comes to tackling these issues, and yet the most common method development approach globally remains scanning gradients using a C18-type column.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In this poster we leverage a virtual method development tool to compare the selectivity of a Biphenyl column against several C18-type phases for a panel of pesticide standards. Initial runs were performed to verify modelled retention times against real-world LC-MS\/MS data utilising a Biphenyl phase, RT differences were compared, with results demonstrating no greater than 10.8s across a 14 minute programmed gradient. Various C18-type columns were then similarly modelled, with the Biphenyl column demonstrating enhanced retention and selectivity for molecules containing phenyl rings, and increased retention for compounds which eluted early into the run (which may typically overlap with matrix elements otherwise).<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/Poster_2025-HPLC_Comparing-C18-Type-Stationary-Phases-to-Biphenyl.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of Poster_2025 HPLC_Comparing C18-Type Stationary Phases to Biphenyl.\"><\/object><a id=\"wp-block-file--media-f3c327f5-82e3-4abe-acf9-6122c1a1a1de\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/Poster_2025-HPLC_Comparing-C18-Type-Stationary-Phases-to-Biphenyl.pdf\">Poster_2025 HPLC_Comparing C18-Type Stationary Phases to Biphenyl<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/Poster_2025-HPLC_Comparing-C18-Type-Stationary-Phases-to-Biphenyl.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-f3c327f5-82e3-4abe-acf9-6122c1a1a1de\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>As LC-MS\/MS continues to grow as the dominant separation technique within a host of industries, more laboratories are trying to run broad panels of analytes from single injections, requiring robust chromatography capable of separating analytes, isobars, and matrix elements suitably to afford suitable detector sensitivity for qualification and quantitation. Column selection is the single most impactful factor when it comes to tackling these issues, and yet the most common method development approach globally remains scanning gradients using a C18-type column.<\/p>\n<p>In this poster we leverage a virtual method development tool to compare the selectivity of a Biphenyl column against several C18-type phases for a panel of pesticide standards. Initial runs were performed to verify modelled retention times against real-world LC-MS\/MS data utilising a Biphenyl phase, RT differences were compared, with results demonstrating no greater than 10.8s across a 14 minute programmed gradient. Various C18-type columns were then similarly modelled, with the Biphenyl column demonstrating enhanced retention and selectivity for molecules containing phenyl rings, and increased retention for compounds which eluted early into the run (which may typically overlap with matrix elements otherwise).<\/p>\n","protected":false},"author":23,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[443,459],"class_list":["post-60341","post","type-post","status-publish","format-standard","hentry","category-posters-presentations"],"acf":[],"taxonomy_info":{"category":[{"value":12,"label":"Posters &amp; Presentations"}]},"featured_image_src_large":false,"author_info":{"display_name":"John Gallant","author_link":"https:\/\/discover.restek.com\/fr\/author\/john-gallant\/"},"comment_info":0,"category_info":[{"term_id":12,"name":"Posters &amp; Presentations","slug":"posters-presentations","term_group":0,"term_taxonomy_id":12,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":12,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations","category_parent":0}],"tag_info":false,"authors":[{"term_id":443,"user_id":23,"is_guest":0,"slug":"john-gallant","display_name":"John Gallant","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-gallant-john.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-gallant-john.jpg"},"author_category":"1","first_name":"John","last_name":"Gallant","user_url":"","job_title":"","description":"John holds a master's degree in chemistry from the University of Sheffield with a thesis on the very interesting topic of energetic materials (typically high nitrogen content chemical compounds\u2013traditionally explosives or solid fuels).\u00a0He previously worked in the QC lab of Ecolab (manufacturer of Licensed Medicinal Products, and Medical Devices) before becoming a field engineer at JayTee Biosciences where he mainly covered HPLC and GC maintenance for instruments from Agilent, Waters, Thermo, and Shimadzu, covering a large area in the UK. Later, he joined Agilent as a remote support engineer, where he helped customers to solve their problems with instrumentation. Troubleshooting chromatography methods was one important part of this work. John is now an LC Specialist for Restek in the United Kingdom."},{"term_id":459,"user_id":39,"is_guest":0,"slug":"melinda-urich","display_name":"Melinda Urich","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-ulrich-melinda.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-ulrich-melinda.jpg"},"author_category":"1","first_name":"Melinda","last_name":"Urich","user_url":"https:\/\/www.linkedin.com\/in\/melinda-urich-997b81225\/","job_title":"","description":"Melinda \u201cMel\u201d Urich is an applications scientist in the LC Solutions department. Her primary focus is on the development of novel applications in the cannabis and food markets. In her previous role at Restek as an LC manufacturing chemist, she led the synthesis of silica, bonding of stationary phases as well as new process implementations and improvements. Mel attended Juniata College where she earned her BS in Chemistry and performed research in Atomic Force Microscopy AFM)."}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/60341","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/23"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=60341"}],"version-history":[{"count":1,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/60341\/revisions"}],"predecessor-version":[{"id":60346,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/60341\/revisions\/60346"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=60341"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=60341"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=60341"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=60341"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=60341"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=60341"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=60341"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=60341"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=60341"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}