{"id":76397,"date":"2021-03-24T00:00:00","date_gmt":"2021-03-24T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76397"},"modified":"2026-02-06T13:52:59","modified_gmt":"2026-02-06T13:52:59","slug":"antiviral-analysis-remdesivir-and-its-metabolites","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/blogs\/gnbl5467\/antiviral-analysis-remdesivir-and-its-metabolites","title":{"rendered":"Antiviral Analysis: Remdesivir and its Metabolites"},"content":{"rendered":"\n<p>Remdesivir is a broad-spectrum antiviral drug that has shown promise in the treatment of Ebola, SARS, Marburg, MERS, and SARS-CoV-2 (COVID-19).<sup>1<\/sup>&nbsp;It is marketed under the trade name of VEKLURY and advertised as \u201cthe first and only antiviral treatment FDA approved for COVID-19.\u201d<sup>2<\/sup>&nbsp;In the body, Remdesivir is metabolized to GS-704277, GS-441524, and GS-443902, with GS-441524 being the major metabolite.<sup>3<\/sup>&nbsp;GS-441524 is less effective than the activated form of Remdesivir (triphosphate GS-443902) for the treatment of Ebola, Hepatitis C, and respiratory syncytial virus, but showed good efficacy&nbsp;<em>in vitro<\/em>&nbsp;against SARS-CoV and MERS-CoV.<sup>3<\/sup><\/p>\n\n\n\n<p>Figure 1. Metabolic pathway proposed for Remdesivir.<sup>3<\/sup><\/p>\n\n\n<style>.kb-image76397_646750-ae:not(.kb-image-is-ratio-size) .kb-img, .kb-image76397_646750-ae.kb-image-is-ratio-size{padding-top:var(--global-kb-spacing-xs, 1rem);padding-bottom:var(--global-kb-spacing-xs, 1rem);}.kb-image76397_646750-ae .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image76397_646750-ae size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"442\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-01-1024x442.png\" alt=\"\" class=\"kb-img wp-image-77041\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-01-1024x442.png 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-01-300x130.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-01-768x332.png 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-01.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Looking at these structures, the first thing I notice is the presence of phosphate groups. Compounds containing phosphate groups can potentially chelate and interact with metal components in your LC system. The best way to overcome this obstacle is to passivate the system before use. The use of medronic acid is a popular technique for system passivation. Restek offers a ready-made passivation solution for your convenience (<a href=\"https:\/\/www.restek.com\/p\/32475\/\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/p\/32475\/\" target=\"_blank\" rel=\"noreferrer noopener\">LC Passivation Solution &#8211; Catalog No.&nbsp;32475<\/a>&nbsp;and <a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/480-01-001.pdf\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/wp-content\/uploads\/480-01-001.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">instruction sheet<\/a>).<\/p>\n\n\n\n<p>After passivating the system, a method was developed to analyze Remdesivir, GS-704277 (alanine metabolite), Remdesivir nucleoside monophosphate, and GS-441524 in plasma in 4.5 min. To prepare the sample, 50 \u00b5L of spiked plasma was added to a centrifuge tube. 200 \u00b5L of cold acetonitrile was added to the sample and vortexed. The sample was centrifuged for 10 min. at 4200 rpm, and an aliquot of the supernatant was transferred to a vial. The extract was then evaporated to dryness under a gentle stream of nitrogen. The sample was reconstituted with 100 \u00b5L of water containing 0.1% acetic acid prior to vortexing.<\/p>\n\n\n<figure class=\"wp-block-table table-borderless\">\n<table style=\"border-style: none;\">\n<tbody>\n<tr>\n<td><strong>Column:<\/strong><\/td>\n<td colspan=\"2\">Raptor Biphenyl (cat.# 9309A52)<\/td>\n<\/tr>\n<tr>\n<td><strong>Dimensions:<\/strong><\/td>\n<td colspan=\"2\">50 mm x 2.1 mm ID<\/td>\n<\/tr>\n<tr>\n<td><strong>Particle Size:<\/strong><\/td>\n<td colspan=\"2\">2.7 \u00b5m<\/td>\n<\/tr>\n<tr>\n<td><strong>Guard Column:<\/strong><\/td>\n<td colspan=\"2\">Raptor Biphenyl EXP guard column cartridge 2.7 \u00b5m (cat.# 9309A0252)<\/td>\n<\/tr>\n<tr>\n<td><strong>Temp.:<\/strong><\/td>\n<td colspan=\"2\">30 \u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Conc.:<\/strong><\/td>\n<td colspan=\"2\">Metabolites 500 ppb; Remdesivir 50 ppb<\/td>\n<\/tr>\n<tr>\n<td><strong>Inj. Vol.:<\/strong><\/td>\n<td colspan=\"2\">2 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td><strong>Mobile Phase<\/strong><\/td>\n<td colspan=\"2\">\u00a0<\/td>\n<\/tr>\n<tr>\n<td><strong>A:<\/strong><\/td>\n<td colspan=\"2\">Water, 20 mM ammonium acetate, 0.1% acetic acid<\/td>\n<\/tr>\n<tr>\n<td><strong>B:<\/strong><\/td>\n<td colspan=\"2\">Methanol, 20 mM ammonium acetate, 0.1% acetic acid<\/td>\n<\/tr>\n<tr>\n<td>\n<div><strong>Time (min)<\/strong><\/div>\n<\/td>\n<td>\n<div><strong>Flow (mL\/min)<\/strong><\/div>\n<\/td>\n<td>\n<div><strong>%B<\/strong><\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>0.00<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>0<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>1.50<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>55<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>2.00<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>100<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>3.00<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>100<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>3.01<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>0<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<div>4.50<\/div>\n<\/td>\n<td>\n<div>0.5<\/div>\n<\/td>\n<td>\n<div>0<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td><strong>Ion Mode<\/strong><\/td>\n<td colspan=\"2\">ESI (+)<\/td>\n<\/tr>\n<tr>\n<td><strong>Detector<\/strong><\/td>\n<td colspan=\"2\">MS\/MS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table table table-bordered\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<p><strong>Peaks<\/strong><\/p>\n<\/td><td>\n<p><strong>Retention Time (min)<\/strong><\/p>\n<\/td><td>\n<p><strong>Precursor Ion<\/strong><\/p>\n<\/td><td>\n<p><strong>Product Ion 1<\/strong><\/p>\n<\/td><td>\n<p><strong>Product Ion 2<\/strong><\/p>\n<\/td><td>\n<p><strong>Product Ion 3<\/strong><\/p>\n<\/td><\/tr><tr><td>\n<p>1. Remdesivir nucleoside monophosphate<\/p>\n<\/td><td>\n<p>0.8<\/p>\n<\/td><td>\n<p>372.1<\/p>\n<\/td><td>\n<p>202.1<\/p>\n<\/td><td>\n<p>147.2<\/p>\n<\/td><td>\n<p>229.1<\/p>\n<\/td><\/tr><tr><td>\n<p>2. GS-704277<\/p>\n<\/td><td>\n<p>1.1<\/p>\n<\/td><td>\n<p>443.1<\/p>\n<\/td><td>\n<p>202.2<\/p>\n<\/td><td>\n<p>124.1<\/p>\n<\/td><td>\n<p>229.2<\/p>\n<\/td><\/tr><tr><td>\n<p>3. GS-441524<\/p>\n<\/td><td>\n<p>2.1<\/p>\n<\/td><td>\n<p>292.0<\/p>\n<\/td><td>\n<p>202.1<\/p>\n<\/td><td>\n<p>163.1<\/p>\n<\/td><td>\n<p>147.1<\/p>\n<\/td><\/tr><tr><td>\n<p>4. Remdesivir<\/p>\n<\/td><td>\n<p>2.9<\/p>\n<\/td><td>\n<p>603.2<\/p>\n<\/td><td>\n<p>200.1<\/p>\n<\/td><td>\n<p>229.1<\/p>\n<\/td><td>\n<p>402.2<\/p>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n<style>.kb-image76397_2a9a9c-d9:not(.kb-image-is-ratio-size) .kb-img, .kb-image76397_2a9a9c-d9.kb-image-is-ratio-size{padding-top:var(--global-kb-spacing-xs, 1rem);padding-bottom:var(--global-kb-spacing-xs, 1rem);}.kb-image76397_2a9a9c-d9 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image76397_2a9a9c-d9 size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"532\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-02.png\" alt=\"\" class=\"kb-img wp-image-77035\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-02.png 1000w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-02-300x160.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-antiviral-analysis-remdesivir-and-its-metabolites-02-768x409.png 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/figure>\n\n\n\n<p>This method of analysis is enabled by the use of a Raptor Biphenyl 50 x 2.1 mm, 2.7 \u00b5m column. Good selectivity and excellent peak shapes were observed for Remdesivir and three of its metabolites in human plasma in an analysis time of only 4.5 min. Is your lab currently running or interested in this analysis? <a href=\"https:\/\/www.restek.com\/contact-us\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/contact-us\" target=\"_blank\" rel=\"noreferrer noopener\">Let us know!<\/a><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Malin JJ, Su\u00e1rez I, Priesner V, F\u00e4tkenheuer G, Rybniker J. Remdesivir against COVID-19 and Other Viral Diseases. Clin Microbiol Rev. 2020 Oct 14;34(1):e00162-20. doi: 10.1128\/CMR.00162-20. PMID: 33055231; PMCID: PMC7566896. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33055231\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/pubmed.ncbi.nlm.nih.gov\/33055231\/<\/a><\/li>\n\n\n\n<li><a data-uw-rm-brl=\"false\" aria-label=\"https:\/\/www.veklury.com\/?gclid=EAIaIQobChMI6LXDvKni7gIVPweICR1FEwI7EAAYAiAAEgJTGfD_BwE&amp;gclsrc=aw.ds - opens in new tab\" data-uw-rm-ext-link=\"\" href=\"https:\/\/www.veklury.com\/?gclid=EAIaIQobChMI6LXDvKni7gIVPweICR1FEwI7EAAYAiAAEgJTGfD_BwE&amp;gclsrc=aw.ds\" target=\"_blank\" rel=\"noopener\">https:\/\/www.veklury.com\/?gclid=EAIaIQobChMI6LXDvKni7gIVPweICR1FEwI7EAAYAiAAEgJTGfD_BwE&amp;gclsrc=aw.ds<\/a><\/li>\n\n\n\n<li>YingjunLi,&nbsp;LiuCao,&nbsp;Ge&nbsp;Li,&nbsp;Feng&nbsp;Cong,&nbsp;Yunfeng&nbsp;Li,&nbsp;Jing&nbsp;Sun,&nbsp;Yinzhu&nbsp;Luo,&nbsp;Guijiang&nbsp;Chen, Guanguan&nbsp;Li,&nbsp;Ping&nbsp;Wang,&nbsp;Fan&nbsp;Xing,&nbsp;Yanxi&nbsp;Ji,&nbsp;Jincun&nbsp;Zhao,&nbsp;Yu&nbsp;Zhang,&nbsp;Deyin&nbsp;Guo,&nbsp;Xumu&nbsp;Zhang. Remdesivir Metabolite GS-441524 Effectively Inhibits SARS-CoV-2 Infection in Mice Models. bioRxiv&nbsp;2020.10.26.353300;&nbsp;doi:&nbsp;<a data-uw-rm-brl=\"false\" aria-label=\"https:\/\/doi.org\/10.1101\/2020.10.26.353300 - opens in new tab\" data-uw-rm-ext-link=\"\" href=\"https:\/\/doi.org\/10.1101\/2020.10.26.353300\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1101\/2020.10.26.353300<\/a>&nbsp;<br><a data-uw-rm-brl=\"false\" aria-label=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.10.26.353300v1.full - opens in new tab\" data-uw-rm-ext-link=\"\" href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.10.26.353300v1.full\" target=\"_blank\" rel=\"noopener\">https:\/\/www.biorxiv.org\/content\/10.1101\/2020.10.26.353300v1.full<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">                                Catalog No. 32475                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Solution de Passivation LC, Acide m\u00e9thyl\u00e8nediphosphonique (Acide M\u00e9dronique), 1760 \u00b5g\/ml, m\u00e9thanol (qualit\u00e9 HPLC)\/eau (50:50), ampoule de 1 ml<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A52\">                                Catalog No. 9309A52                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonnes Raptor Biphenyl, 2,7\u00b5m 50 x 2.1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A52\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A0252\">                                Catalog No. 9309A0252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Cartouche de garde Raptor Biphenyl EXP, 2.7 \u00b5m, L 5\u00a0x DI 2.1 mm, lot de 3<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A0252\">Voir le produit<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Remdesivir is a broad-spectrum antiviral drug that has shown promise in the treatment of Ebola, SARS, Marburg, MERS, and SARS-CoV-2 (COVID-19).1 It is marketed under the trade name of VEKLURY and advertised as &ldquo;the first and only antiviral treatment FDA approved for COVID-19.&rdquo; Looking at these structures, the first thing I notice is the presence of phosphate groups. Compounds containing phosphate groups can potentially chelate and interact with metal components in your LC system. The best way to overcome this obstacle is to passivate the system before use.<\/p>\n","protected":false},"author":47,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[623],"ppma_author":[579],"class_list":["post-76397","post","type-post","status-publish","format-standard","hentry","category-blogs"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jamie L. York, PhD","author_link":"https:\/\/discover.restek.com\/fr\/author\/jamie-l-york-phd\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":442,"filter":"raw","cat_ID":9,"category_count":442,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76397","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/47"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=76397"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76397\/revisions"}],"predecessor-version":[{"id":87146,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76397\/revisions\/87146"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=76397"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=76397"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=76397"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=76397"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=76397"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=76397"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=76397"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=76397"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/hf_cat_post?post=76397"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=76397"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}