{"id":76482,"date":"2015-05-20T00:00:00","date_gmt":"2015-05-20T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76482"},"modified":"2025-12-30T23:04:00","modified_gmt":"2025-12-30T23:04:00","slug":"you-want-them-i-got-them-a-lot-of-them-pesticides-gc-msms-transitions","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/blogs-de\/gnbl5560\/you-want-them-i-got-them-a-lot-of-them-pesticides-gc-msms-transitions","title":{"rendered":"You want them, I got them…a lot of them: pesticides GC-MS\/MS transitions"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n\n

I can tell you that determining transitions (SRMs) for GC-MS\/MS can be quite challenging with so many precursor ions to evaluate. I spent a considerable amount of time optimizing transitions for our\u00a0GC Multiresidue Pesticide Kit,\u00a0which has 203 compounds. That is a lot of transitions to evaluate!<\/p>\n\n\n\n

I also tested my chromatographic method for analyte-to-analyte interferences by comparing ion ratios. The ion ratio of qualifying Q3 ion to quantification Q3 ion for each compound tested in small subsets was compared to the ion ratio determined in the presence of all 203 compounds. When the two ratios fell outside of plus\/minus thirty percent, this indicated analyte-to-analyte interference, and alternative transitions were used.<\/p>\n\n\n\n

Transitions for all compounds and notes about analyte to analyte interferences and shared transitions can be found in this\u00a0downloadable spreadsheet<\/a>.\u00a0The data was collected and optimized on our\u00a0Thermo Scientific TSQ 8000 Triple Quadrupole GC-MS System.<\/p>\n\n\n\n

The\u00a0spreadsheet\u00a0provides the method conditions (last tab), retention times, and three SRMs per compound. CAS numbers and isomers information is also included.<\/p>\n\n\n\n

I hope that this helps accelerate your method development!<\/p>\n\n\n\n

<\/div>\n\n\n
\n

Shopping list for this method:<\/h3>\n
\n
\n
\n
\n Catalog No. 32562 <\/a> <\/div>\n
\n
Kit de multi-r\u00e9sidus de pesticides GC<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13423 <\/a> <\/div>\n
\n
Colonne capillaire Rxi-5ms, L 30\u00a0m, DI 0.25 mm, 0.25 \u00b5m<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 23447 <\/a> <\/div>\n
\n
Insert Topaz Gooseneck, 4.0\u00a0mm x 6.5 x 78.5, pour GC Thermo TRACE 1300\/1310, 1600\/1610 avec injecteur SSL, avec laine de quartz, d\u00e9sactivation Premium, lot de 5<\/div>\n <\/div>\n
\n Voir le produit<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n

Internal Standards and\/or  Surrogates<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

I can tell you that determining transitions (SRMs) for GC-MS\/MS can be quite challenging with so many precursor ions to evaluate. I spent a considerable amount of time optimizing transitions for our GC Multiresidue Pesticide Kit which has 203 compounds….<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[792],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[623],"ppma_author":[594],"class_list":["post-76482","post","type-post","status-publish","format-standard","hentry","category-blogs-de"],"acf":[],"taxonomy_info":{"category":[{"value":792,"label":"Blogs"}]},"featured_image_src_large":false,"author_info":{"display_name":"Tari Elkin","author_link":"https:\/\/discover.restek.com\/fr\/author\/tari-elkinrestek-com\/"},"comment_info":0,"category_info":[{"term_id":792,"name":"Blogs","slug":"blogs-de","term_group":0,"term_taxonomy_id":792,"taxonomy":"category","description":"","parent":0,"count":442,"filter":"raw","cat_ID":792,"category_count":442,"category_description":"","cat_name":"Blogs","category_nicename":"blogs-de","category_parent":0}],"tag_info":false,"authors":[{"term_id":594,"user_id":0,"is_guest":1,"slug":"julie-kowalski","display_name":"Julie Kowalski","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76482","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=76482"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76482\/revisions"}],"predecessor-version":[{"id":78930,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/76482\/revisions\/78930"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=76482"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=76482"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=76482"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=76482"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=76482"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=76482"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=76482"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=76482"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/hf_cat_post?post=76482"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=76482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}