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PFAS Analysis by U.S. USDA CLG-PFAS 2.04 for Bovine, Porcine, Poultry, Siluriformes Muscle, and Bovine Plasma

03 Oct 2025

feature FSAR4290
  • Sample Matrix: bovine, porcine, poultry, and Siluriformes muscle and bovine plasma
  • Sample Preparation: protein precipitation extraction
  • Detection Technique: UHPLC-MS/MS

Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS

USDA Method CLG-PFAS 2.04 is used for the analysis of 16 PFAS in bovine, porcine, poultry, Siluriformes muscle samples, and in bovine plasma samples. It combines a methanol-based protein precipitation extraction with UHPLC-MS/MS analysis on a C18 column. The method is performance based and allows equivalent equipment, reagents, or solutions to be used, and some instrument parameters can be optimized as detailed in the method to improve chromatography and detection. The full analyte list and recommended products for PFAS analysis in bovine, porcine, poultry, Siluriformes muscle, and bovine plasma samples by CLG-PFAS 2.04 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for USDA CLG-PFAS 2.04 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. USDA CLG-PFAS 2.04 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFDoAPerfluorododecanoic acid307-55-1
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFTeDAPerfluorotetradecanoic acid376-06-7
PFTrDAPerfluorotridecanoic acid72629-94-8
PFUnAPerfluoroundecanoic acid2058-94-8
PFPeAPerfluoropentanoic acid2706-90-3
PFDSPerfluoro-1-decanesulfonic acid335-77-3
PFHxDAPerfluoro-n-hexadecanoic acid67905-19-5
PFODAPerfluoro-n-octadecanoic acid16517-11-6

Recommended Products for U.S. USDA CLG-PFAS for PFAS Analysis

Analytical LC Columns:


Colonna HPLC Raptor C18, 2,7 µm, 50 x 2,1 mm
Colonna LC Force C18, 1,8 µm, 50 x 2,1 mm

Delay Columns:


PFAS Delay Column, 5 µm, 50 x 2,1 mm, colonna HPLC

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


Filtro precolonna UHPLC UltraShield, frit 0,2 µm, cad.
Holder EXP a connessione diretta per cartucce per precolonne EXP, comprende raccordo e ferrule
Cartuccia per precolonna Raptor C18 EXP, 2,7 µm, 5 x 2,1 mm, 3 pz.

Standards:


Standard di calibrazione PFAS EPA 537.1, 2 μg/mL, metanolo (1 mM KOH), 1 mL/ampolla
Discontinued: Standard di calibrazione PFAS EPA 533, 2 μg/mL, metanolo (1 mM KOH), 1 mL/ampolla

Sample Prep:


Centrifuga multispeed Q-sep per QuEChERS

Sample Reservoirs:


Provette vuote SPE, serbatoio per campioni, 75 mL, polipropilene, 12 pz.
Connettore SPE, 1, 3, 6, 10 o 15 mL, polipropilene, 15 pz.

Vacuum Manifolds:


Vacuum Manifold Resprep QR-24
Vacuum Manifold Resprep QR-12

Vacuum Pumps:


Pompa da vuoto in PTFE Chemker 300, 20 l/min, CA 110 V, 60 Hz
Pompa da vuoto in PTFE Chemker 300, 18 l/min, CA 220 V, 50 Hz

General Lab Supplies:


Vial a volume ridotto in polipropilene con filettatura, 9 mm, 700 µL, 12 x 32 mm, 100 pz.
Vial a volume ridotto in polipropilene con filettatura, 9 mm, 700 µL, 12 x 32 mm, 1000 pz.
Tappi in polietilene da 2,0 mL con top solido di 9 mm, filettatura, membrana spessore 10 mm, trasparente, 100 pz.
Tappi in polietilene da 2,0 mL con top solido di 9 mm, filettatura, membrana spessore 10 mm, trasparente, 1000 pz.
Provette da centrifuga vuote da 50 ml, Polipropilene con tappo, 50 provette, 50-conf.
Provette da centrifuga vuote da 50 ml, Polipropilene con tappo, 50 provette, 500-conf.
Siringa di Plastica Norm-Ject, Luer Lock Tip, graduazione fino a 12 mL, 10 mL, Conf. da 100
Siringa di Plastica Norm-Ject, Luer Lock Tip, graduazione fino a 6 mL, 5 mL, Conf. da 100

Discover More Solutions for PFAS Analysis!

EZLC modeler PFAS Placeholder Image

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  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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FSAR4290
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