Analysis of FAMEs in Biodiesel Fuel: Pro EZGC Modeling Software Ensures Proper Column Selection

Analysis of FAMEs in Biodiesel Fuel: Pro EZGC Modeling Software Ensures Proper Column Selection

ByAudrey Crater

Polar columns were evaluated for the analysis of fatty acids methyl esters (FAMEs) in finished B100 biodiesel according to method EN 14103 (2011). Using Restek’s Pro EZGC chromatogram modeler, a high cyano phase Rt-2330 column and a polyethylene glycol phase FAMEWAX column were compared. The modeling software predicted an unacceptable coelution between the internal standard (C19:0 FAME) and FAME C18:2 when using the Rt-2330 column. However, the modeler also predicted that the FAMEWAX column would separate all the compounds of interest, which was demonstrated empirically. In addition, the results on the FAMEWAX column showed excellent repeatability for both total FAMEs and the linolenic acid methyl ester component.

Analysis of Biodiesel Fatty Acid Methyl Esters (FAME) in Aviation Jet Fuels Using Method IP 585

Analysis of Biodiesel Fatty Acid Methyl Esters (FAME) in Aviation Jet Fuels Using Method IP 585

ByAudrey Crater

Accurate analysis of biodiesel FAME in jet fuel is critical for ensuring that contamination levels do not exceed allowable limits. Method IP 585 is commonly used for this analysis and here we perform the method using a Stabilwax column and GC-MS in SIM mode. Method calibration curve criteria were met and good recoveries of the total FAME content in jet fuel were obtained.

Alternative Carrier Gases for ASTM D7213 Simulated Distillation Analysis

Alternative Carrier Gases for ASTM D7213 Simulated Distillation Analysis

ByAudrey Crater

Carrier gas changes for SimDist methods, such as ASTM Method D7213, can be easily implemented in your daily process. Using MXT-1HT SimDist columns and EZGC method translation software, helium-based SimDist methods can be easily translated to alternative carrier gases while maintaining ASTM Method D7213 requirements.

Characterizing Cellular Fatty Acid Methyl Ester (FAME) Profiles to Identify Bacteria Using Gas Chromatography

ByAudrey Crater

Dr. Radomír Čabala, Head of the Toxicology Department at the General University Hospital in Prague, presents work on the potential utility of GC-TOFMS analysis of cellular fatty acid methyl esters (FAMEs) in identifying clinically relevant bacteria.

Two Options for Analyzing Potential Genotoxic Impurities in Active Pharmaceutical Ingredients

ByAudrey Crater

Two options for the analysis of PGIs in API have been developed by Merck and Restek to meet different laboratory needs. The first option is a fast method for the analysis of sulfonate esters on the Rxi-5Sil MS column. The second option is a comprehensive method for the analysis of both sulfonate esters and alkyl halides on the Rtx-200 column. Both methods require very little sample preparation, which helps increase laboratory productivity.

Retention Cross-over Phenomenon in Gas Chromatography — Can the Mystery be Revealed?

ByAudrey Crater

Dr. Werner Engewald discusses the phenomenon of cross-over, or changes in elution order following modification of the GC temperature program. This effect has been observed for decades but the physio-chemical background still is not well understood. In this article Dr. Engewald explores the theory and potential explanations for the cross-over phenomenon.

Achieving Faster GC

ByAudrey Crater

Numerous articles have been published on faster methods for gas chromatography (GC), yet uncertainty remains on how best to speed up separations. Here, Dr. Hans-Gerd Janssen clarifies the confusion surrounding fast GC and recommends a strategic approach based on 3 steps: 1) minimizing resolution to a value just sufficient, 2) maximizing the selectivity of the chromatographic system, and 3) implementing a method that reduces analysis time while holding resolution constant.