Over the years, Restek has run applications on a long list of columns for this analysis. It does require a primary and secondary column for analysis, since there is not one column that perfectly separates all compounds on the list simultaneously. Keep in mind that EPA Method 8330B allows for alternate columns versus the ones listed if you can demonstrate proficiency by presenting valid data that meets QC acceptance criteria as described in Method 8000. Our Innovations chemists have worked hard to make column selection easier for this and get you started in the right direction for optimization.
So far, we have determined optimal conditions for two pairs of columns that we recommend above all other combinations. For traditional HPLC systems with a 400 Bar pressure limit, we suggest using an Ultra C8 as the primary column and an Ultra Aromax as the secondary (confirmatory) column.
Here are chromatograms.
Explosives by EPA 8330B on Ultra C8 with Trident Direct Guard Column
LC_EV0526
Conditions
| Ultra C8 (cat.# 9103565) |
| 150 mm x 4.6 mm ID |
| 5 µm |
| 100 Å |
|
| 30 °C |
| 8330B Nitroaromatics and nitramine mix (cat.# 33204) |
| 1,4-Dinitrobenzene (cat.# 33205) |
| Deionized water |
| 20 µg/mL |
| | 10 µL |
| 1.2 mL/min |
Explosives by EPA 8330B on Ultra Aromax with Trident Direct Guard
LC_EV0527
Conditions
| Ultra Aromax (cat.# 9127565) |
| 150 mm x 4.6 mm ID |
| 5 µm |
| 100 Å |
|
| 35 °C |
| 8330B Nitroaromatics and nitramine mix (cat.# 33204) |
| 1,4-Dinitrobenzene (cat.# 33205) |
| Deionized water |
| 20 µg/mL |
| | 10 µL |
| 1.2 mL/min |
If you have a system that has a pressure limit of at least 600 Bar, we suggest our Raptor Biphenyl as the primary column and Raptor ARC-18 as the secondary column. Here are chromatograms.
Explosives on Raptor Biphenyl by EPA Method 8330B
LC_EV0531
Conditions
| Raptor Biphenyl (cat.# 9309A65) |
| 150 mm x 4.6 mm ID |
| 2.7 µm |
|
| 35 °C |
| 8330B Nitroaromatics and nitramine mix (cat.# 33204) |
| 1,4-Dinitrobenzene (cat.# 33205) |
|
| Water |
| 20 µg/mL |
| | 5 µL |
| Water |
| Methanol |
| | Time (min) | Flow (mL/min) | %A | %B |
|---|
| 0.00 | 1.0 | 30 | 70 | | 10 | 1.0 | 30 | 70 |
|
| UV/Vis @ 210/254 nm |
| 35 °C |
| Semi-micro |
Explosives on Raptor ARC-18 by EPA Method 8330B
LC_EV0530
Conditions
| Raptor ARC-18 (cat.# 9314A1E) |
| 100 mm x 3.0 mm ID |
| 2.7 µm |
|
| 35 °C |
| 8330B Nitroaromatics and nitramine mix (cat.# 33204) |
| 1,4-Dinitrobenzene (cat.# 33205) |
|
| Water |
| 20 µg/mL |
| | 3 µL |
| Water |
| Methanol |
| | Time (min) | Flow (mL/min) | %A | %B |
|---|
| 0.00 | 1.0 | 75 | 25 | | 22.5 | 1.0 | 75 | 25 |
|
| UV/Vis @ 210/254 nm |
| 35 °C |
| Semi-micro |
I hope this clarifies some things and gets you started in the right direction. Thank you for reading.
Products Mentioned
Colonna HPLC Ultra C8, 5 µm, 150 x 4,6 mm
Cartuccia per precolonna Ultra C8, 5 µm, 10 x 4,0 mm, 3 pz.
Colonna HPLC Ultra Aromax, 5 µm, 150 x 4,6 mm
Cartuccia per precolonna Ultra Aromax, 5 µm, 10 x 4,0 mm, 3 pz.
Colonna HPLC Raptor ARC-18, 2,7 µm, 100 x 3,0 mm
Colonna HPLC Raptor bifenilica, 2,7 µm, 150 x 4,6 mm
Miscela di esplosivi nitramminici e nitroaromatici 8330B con HPLC, 1000 µg/mL, acetonitrile, 1 mL/ampolla
Standard interno 8330 (1,4-dinitrobenzene) 2000 µg/mL, acetonitrile, 1 mL/ampolla
