Method 505: Analysis of Organohalide Pesticides and Commercial PCBs in Water by Microextraction and GC

With the advent of modern agriculture and its vast selection of chemical pest control measures, the farming community has made significant increases in productivity and efficiency. Crop yield per acre is extremely high, due in part to the role of pesticides and herbicides in mitigating the devastating effects of many plant and insect pests. However, the use of these chemicals can have drawbacks, including surface and ground water contamination. EPA Method 505 is used for organohalide pesticide analysis in drinking and ground water. The Rtx-CLPesticides and Rtx-CLPesticides2 column set works well for the analysis of organohalide compounds because the columns are highly selective for analytes that contain electronegative substituents. The optimized parallel dual-column method shown here provides fast analysis times, which improves sample throughput.

Comparison based on published competitor data. All columns tested were 0.32 mm ID.

Organohalide pesticide analysis (Method 505) on Rtx-CLPesticides and Rtx-CLPesticides2 columns.

GC_EV01004

Peaks

	Peaks
1.	Hexachlorocyclopentadiene
2.	Hexachlorobenzene
3.	Simazine
4.	Atrazine
5.	γ-BHC
6.	Heptachlor
7.	Alachlor
8.	Aldrin

	Peaks
9.	Heptachlor epoxide
10.	trans-Chlordane
11.	trans-Nonachlor
12.	cis-Chlordane
13.	Dieldrin
14.	Endrin
15.	cis-Nonachlor
16.	Methoxychlor

Conditions

Columns	Rtx-CLPesticides 30 m, 0.32 mm ID, 0.32 µm (cat.# 11141)
	and Rtx-CLPesticides2 30 m, 0.32 mm ID, 0.25 µm (cat.# 11324)
	using Rxi guard column 5 m, 0.32 mm ID (cat.# 10039)
	with deactivated universal “Y” Press-Tight connector (cat.# 20405-261)
Standard/Sample
	200 ng/mL 505 organohalide pesticide mix (cat.# 32024)
	4.2 µg/mL simazine (cat.# 32236)
	4.2 µg/mL atrazine (cat.# 32208)
Diluent:	Methanol
Injection
Inj. Vol.:	2 µL splitless (hold 0.75 min)
Liner:	Cyclo double taper (4 mm) (cat.# 20896)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	90 °C (hold 1 min) to 310 °C at 10 °C/min (hold 5 min)
Carrier Gas	He, constant flow
Linear Velocity:	40 cm/sec

Detector	Micro-ECD @ 325 °C
Instrument	Agilent/HP6890 GC
Notes	This chromatogram was obtained using an Agilent micro-ECD. To obtain comparable results, you will need to employ a micro-ECD in addition to confirmational dual columns connected to a 5-meter guard column using a “Y” Press-Tight connector.

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Products Mentioned

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Catalog No. 11196

Kit colonna capillare GC Rtx-CLPesticides, ID 0,32 mm, 0,32 µm

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Catalog No. 11120

Colonna capillare GC Rtx-CLPesticides, 15 m, ID 0,25 mm, 0,25 µm

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Catalog No. 11321

Colonna capillare GC Rtx-CLPesticides2, 15 m, ID 0,32 mm, 0,25 µm

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