- Sample Matrix: food and feed (validated in lettuce, chocolate milk, salmon, bread, eggs, clams, blueberries, silage, and corn snaplage)
- Sample Preparation: QuEChERS, filtration, solid-phase extraction
- Detection Technique: LC-MS/MS
Determination of 30 Per- and Polyfluoroalkyl Substances (PFAS) in Food and Feed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
U.S. FDA Method C-010.03 is used for the analysis of 30 PFAS in food and feed. It combines QuEChERS, filtration, and solid phase extraction sample preparation with LC-MS/MS analysis on a C18 column. The method also uses LC-HRMS for verification of PFBA and PFPeA detection in order to address the potential for false positives due to matrix interferences since these compounds each have only one MS/MS transition. The full analyte list and recommended products for PFAS analysis in food and feed by Method C-010.03 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for FDA C-010.03 for PFAS Samples
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. FDA C-010.03 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
| PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| PFBA | Perfluorobutanoic acid | 375-22-4 |
| PFPeA | Perfluoropentanoic acid | 2706-90-3 |
| PFDS | Perfluoro-1-decanesulfonic acid | 335-77-3 |
| PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
| PFPeS | Perfluoro-1-pentanesulfonic acid | 2706-91-4 |
| FOSA | Perfluoro-1-octanesulfonamide | 754-91-6 |
| 4:2 FTS | 1H,1H,2H,2H-Perfluorohexane sulfonic acid | 757124-72-4 |
| 6:2 FTS | 1H,1H,2H,2H-Perfluorooctane sulfonic acid | 27619-97-2 |
| 8:2 FTS | 1H,1H,2H,2H-Perfluorodecane sulfonic acid | 39108-34-4 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| 11Cl-PF3OUdS | 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) | 763051-92-9 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
| PFNS | Perfluoro-1-nonanesulfonic acid | 68259-12-1 |
| PFDoS | Perfluorododecane sulfonate | 79780-39-5 |
| 10:2 FTS | 1H,1H,2H,2H-perfluorododecane sulfonate (10:2) | 120226-60-0 |
| PFUnDS | Perfluoroundecane sulfonic acid | 749786-16-1 |
| PFTrDS | Perfluorotridecane sulfonic acid | 791563-89-8 |
Recommended Products for FDA C-010.03 for PFAS Analysis
Analytical LC Columns:
PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
Guard Columns:
Sample Prep:
Thomson SINGLE StEP nano filter vials: 0.2 µm, nylon w/preslit cap (cat.# 25886) (Note that this U.S. FDA method uses nano filter vial without caps. Cat#. 25886 comes with preslit caps, but polyethylene caps (cat.#s 23244 and 23247) can be ordered separately.)
Vacuum Manifolds:
Vacuum Pumps:
General Lab Supplies:
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