Please follow the recommendations in our blog “Building up Pressure on HPLC?” This will help you identify or confirm the source of pressure. If the column itself is found to be the source of difficulty, please follow the column regeneration steps given on our LC Column Cleaning Recommendations page. To help avoid future occurrences, Restek highly recommends the use of guard columns to protect your reversed-phase and normal-phase analytical LC columns from both frit blockage and column contamination.
How do I address an increase in back pressure and other signs of blockage or contamination?
31 Jul 2025
Author
GNOT5055
