Yes, you can use a variety of solvents. Resprep PPT3 96-well plates are made of high-quality polypropylene housing and solvent-resistant membrane materials. Many standard solvents, such as methanol, dichloromethane etc. can be used, but different solvents will produce different protein precipitation results. Acetonitrile is recommended because it is readily available in most laboratories, and it provides strong protein precipitation capability for samples that contain high levels of protein (e.g., plasma).
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