{"id":42435,"date":"2019-10-01T04:00:00","date_gmt":"2019-10-01T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/condizionare-la-colonna-e-lo-strumento-lc-per-metodi-hilic\/"},"modified":"2025-12-20T15:56:52","modified_gmt":"2025-12-20T15:56:52","slug":"condizionare-la-colonna-e-lo-strumento-lc-per-metodi-hilic","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/video\/gnav3575-it\/condizionare-la-colonna-e-lo-strumento-lc-per-metodi-hilic","title":{"rendered":"Condizionare la Colonna e lo Strumento LC per Metodi HILIC"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Conditioning Your LC Instrument and Column for HILIC\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/znIAVFtVjOI?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<p>Sottotitoli disponibili in italiano<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Descrizione<\/h2>\n\n\n\n<p>La cromatografia liquida a interazione idrofilica, o HILIC, \u00e8 un\u2019efficace tecnica della cromatografia liquida (LC) che utilizza fasi stazionarie polari e condizioni iniziali della fase mobile ad elevata percentuale di solvente organico per la separazione degli analiti polari. I tamponi sono molto importanti per nei metodi HILIC perch\u00e9 lo stato di ionizzazione, sia degli analiti che della fase stazionaria, avr\u00e0 un impatto sulla separazione. Qui vedremo quali sono gli step per ottimizzare le concentrazioni del tampone.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Altre Risorse<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/discover.restek.com\/gnar2716\/how-to-avoid-common-problems-with-hilic-methods\/\" data-type=\"post\" data-id=\"43644\">How to Avoid Common Problems with HILIC Methods<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/discover.restek.com\/gnss2687\/raptor-hilic-si-simplify-the-switch-to-hilic\/\" data-type=\"post\" data-id=\"43822\">Simplify the Switch to HILIC with Raptor HILIC-Si Columns<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Strumenti e ricambi raccomandati da restek<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/global\/it\/search\/hilic-si\/_\/N-1673632971?Ns=restekProduct.x_productBadge%7C0\" target=\"_blank\" rel=\"noopener\">Raptor HILIC-Si Columns<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>In this Restek Tip it\u2019s time to condition your column and instrument for HILIC. Liquid chromatography.<\/p>\n","protected":false},"author":11,"featured_media":41026,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[773],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2535,2517],"resource-technique":[2334,2356],"ppma_author":[414],"class_list":["post-42435","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-video","product-library-colonne-lc","product-library-prodotti-per-cromatografia-liquida","resource-technique-cromatografia-liquida-lc","resource-technique-hilic-it"],"acf":[],"taxonomy_info":{"category":[{"value":773,"label":"Video"}],"product-library":[{"value":2535,"label":"Colonne LC"},{"value":2517,"label":"Prodotti per Cromatografia Liquida"}],"resource-technique":[{"value":2334,"label":"Cromatografia Liquida (LC)"},{"value":2356,"label":"HILIC"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3575-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/it\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":773,"name":"Video","slug":"video","term_group":0,"term_taxonomy_id":773,"taxonomy":"category","description":"","parent":0,"count":133,"filter":"raw","cat_ID":773,"category_count":133,"category_description":"","cat_name":"Video","category_nicename":"video","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=42435"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42435\/revisions"}],"predecessor-version":[{"id":76102,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42435\/revisions\/76102"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media\/41026"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=42435"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=42435"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=42435"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=42435"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=42435"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=42435"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=42435"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=42435"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=42435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}