{"id":42488,"date":"2018-01-03T05:00:00","date_gmt":"2018-01-03T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-troubleshooting-baseline-problems\/"},"modified":"2026-01-28T15:55:52","modified_gmt":"2026-01-28T15:55:52","slug":"lc-troubleshooting-baseline-problems","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/videos-ja\/gnav3620\/lc-troubleshooting-baseline-problems","title":{"rendered":"LC Troubleshooting\u2014Baseline Problems"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Troubleshooting\u2014Baseline Problems\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/JrEs89ltQUE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n<div class=\"row\">\n<div class=\"col-12\">\n<h2 aria-level=\"3\"><span data-contrast=\"none\">Description<\/span><\/h2>\n<p><span data-contrast=\"auto\">Trouble with your liquid chromatography&nbsp;<\/span><span data-contrast=\"auto\">(LC)&nbsp;<\/span><span data-contrast=\"auto\">baseline? We share some common examples of problematic baselines that we might see with some&nbsp;<\/span><span data-contrast=\"auto\">non-MS<\/span><span data-contrast=\"auto\">&nbsp;LC detectors<\/span><span data-contrast=\"auto\">.&nbsp;<\/span><span data-contrast=\"auto\">Baseline problems may be erratic such as those caused by an air bubble, whereas a more systematic pattern would be more likely to be a pump or piston problem. Finally, a generally bad looking baseline could be telling us it is time to clean our system.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">&nbsp;<\/span><\/p>\n<h3>Additional Resources<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.restek.com\/articles\/restek-liquid-chromatography-lc-columns-cleaning-recommendations\" target=\"_blank\" rel=\"noopener\"><span data-contrast=\"auto\">Restek Liquid Chromatography Column Cleaning Recommendations<\/span><\/a><\/li>\n<\/ul>\n<h3>Restek Recommended Tools &amp; Parts<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.restek.com\/c\/1125\" target=\"_blank\" rel=\"noopener\"><span data-contrast=\"auto\">LC Maintenance Supplies<\/span><\/a><\/li>\n<\/ul>\n<div>\n<h3>Connect with Us<\/h3>\n<p><span data-contrast=\"auto\">YouTube:&nbsp;<\/span><a href=\"https:\/\/www.youtube.com\/user\/RestekCorp\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"exc\" aria-label=\"https:\/\/www.youtube.com\/user\/RestekCorp - opens in new tab\" data-uw-rm-ext-link=\"\" uw-rm-external-link-id=\"https:\/\/www.youtube.com\/user\/restekcorp$https:\/\/www.youtube.com\/user\/restekcorp\"><span data-contrast=\"none\">https:\/\/www.youtube.com\/user\/RestekCorp<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">&nbsp;<br role=\"presentation\" data-uw-rm-sr=\"\"><\/span><span data-contrast=\"auto\">LinkedIn:&nbsp;<a href=\"https:\/\/www.linkedin.com\/company\/restek-corporation\/\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"exc\" aria-label=\"https:\/\/www.linkedin.com\/company\/restek-corporation\/ - opens in new tab\" data-uw-rm-ext-link=\"\" uw-rm-external-link-id=\"https:\/\/www.linkedin.com\/company\/restek-corporation\/$https:\/\/www.linkedin.com\/company\/restek-corporation\/\">https:\/\/www.linkedin.com\/company\/restek-corporation\/<\/a><br role=\"presentation\" data-uw-rm-sr=\"\">Facebook:&nbsp;<a href=\"https:\/\/www.facebook.com\/RestekCorporation\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"false\" aria-label=\"https:\/\/www.facebook.com\/RestekCorporation - opens in new tab\" data-uw-rm-ext-link=\"\" uw-rm-external-link-id=\"https:\/\/www.facebook.com\/restekcorporation$https:\/\/www.facebook.com\/restekcorporation\">https:\/\/www.facebook.com\/RestekCorporation<\/a><\/span><\/p>\n<\/div>\n<h2 aria-level=\"3\">Transcript<\/h2>\n<p><span data-contrast=\"auto\">Thanks for joining us for the latest Restek Tip. Today, I&#8217;m going to talk about troubleshooting LC baseline. There are a couple patterns that we can look at that can help us diagnose some problems.&nbsp;<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">If we start to see something that&#8217;s very erratic, this could be indicative that you have an air bubble in your system and simply need to purge it with mobile phase to get back to normal.&nbsp;<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Secondly, if you start to see something a bit more systematic, this could be a pump or piston issue, so simply by doing some routine maintenance in your laboratory, you can get your system back up and running to normal.&nbsp;<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Lastly, if you start to see something a bit more spurious, it can be a bit more difficult to diagnose, but typically by doing a thorough system cleaning, it can get you back to normal and you get your instrument back up and running soon.<\/span><\/p>\n<\/div>\n<\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>LC baseline problems? Learn what to look for and how to fix them!<\/p>\n","protected":false},"author":11,"featured_media":41166,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[774],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2517],"resource-technique":[2334],"ppma_author":[414],"class_list":["post-42488","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-ja","product-library-prodotti-per-cromatografia-liquida","resource-technique-cromatografia-liquida-lc"],"acf":[],"taxonomy_info":{"category":[{"value":774,"label":"\u52d5\u753b"}],"product-library":[{"value":2517,"label":"Prodotti per Cromatografia Liquida"}],"resource-technique":[{"value":2334,"label":"Cromatografia Liquida (LC)"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3620-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/it\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":774,"name":"\u52d5\u753b","slug":"videos-ja","term_group":0,"term_taxonomy_id":774,"taxonomy":"category","description":"","parent":0,"count":134,"filter":"raw","cat_ID":774,"category_count":134,"category_description":"","cat_name":"\u52d5\u753b","category_nicename":"videos-ja","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=42488"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42488\/revisions"}],"predecessor-version":[{"id":85371,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/42488\/revisions\/85371"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media\/41166"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=42488"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=42488"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=42488"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=42488"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=42488"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=42488"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=42488"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=42488"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=42488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}