{"id":43488,"date":"2026-08-28T13:38:50","date_gmt":"2026-08-28T13:38:50","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/innovativa-fase-stazionaria-per-unanalisi-completa-dei-pfas-composti-a-catena-ultra-corta-c2-c3-alternativi-e-tradizionali\/"},"modified":"2026-04-29T13:49:07","modified_gmt":"2026-04-29T13:49:07","slug":"innovativa-fase-stazionaria-per-unanalisi-completa-dei-pfas-composti-a-catena-ultra-corta-c2-c3-alternativi-e-tradizionali","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/articoli\/evfa3197-it\/innovativa-fase-stazionaria-per-unanalisi-completa-dei-pfas-composti-a-catena-ultra-corta-c2-c3-alternativi-e-tradizionali","title":{"rendered":"Innovativa fase stazionaria per un\u2019analisi completa dei PFAS: composti a catena ultra-corta (C2, C3), alternativi e tradizionali"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Una fase stazionaria unica per una corretta ritenzione cromatografica di PFAS piccoli, polari a catena ultra-corta e di PFAS a catena corta e a catena lunga.<\/li>\n\n\n\n<li>Un metodo LC-MS\/MS isocratico facile e veloce che permette un\u2019elevata produttivit\u00e0 nell\u2019analisi dei PFAS.<\/li>\n\n\n\n<li>Una prestazione costante della colonna garantisce risultati accurati.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Gli attuali metodi LC-MS\/MS per l\u2019analisi di sostanze per- e polifluoroalchiliche (PFAS) sono centrati sui composti a catena corta (C4-C6), a catena lunga (C8 e oltre) e alternativi o di sostituzione, e non includono i composti a catena ultra-corta ora maggiormente diffusi (C2 e C3). I PFAS a catena ultra-corta stanno acquisendo sempre maggiore importanza perch\u00e9 sono spesso presenti nelle acque ambientali (per es., pioggia, fiumi e acque sotterranee) e si \u00e8 riscontrato che compongono almeno il 40% dei PFAS totali nei campioni d\u2019acqua. I PFAS a catena ultra-corta comprendono l\u2019acido trifluoroacetico (TFA), che \u00e8 il pi\u00f9 abbondante e al contempo uno dei pi\u00f9 difficili da analizzare, l\u2019acido perfluoropropionico (PFPrA), l\u2019acido perfluoroetansolfonico (PFEtS) e l\u2019acido perfluoropropansolfonico (PFPrS). Per un programma di monitoraggio dell\u2019acqua \u00e8 di fondamentale importanza la completezza dei metodi di analisi dei PFAS, che devono garantire risultati affidabili per i composti a catena ultracorta (TFA incluso) e per i PFAS alternativi e tradizionali.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">L\u2019aspetto critico dell\u2019analisi del TFA \u00e8 la ritenzione limitata e\/o la scarsa resa cromatografica sulle colonne a fase inversa e HILIC. Altre colonne che enfatizzano forti meccanismi di scambio ionico, invece, presentano il problema opposto, ovvero troppa ritenzione e forme del picco di bassa qualit\u00e0. Invece, il TFA pu\u00f2 essere trattenuto e analizzato in maniera affidabile sulle colonne Raptor Polar X grazie alla presenza di un unico ligando ibrido che combina i meccanismi di ritenzione HILIC e a scambio ionico. Come qui illustrato, \u00e8 stato delineato un metodo LC-MS\/MS facile e veloce per l\u2019analisi completa dei PFAS, compresi C2, C3, C4, C6, C8 e i PFAS alternativi. \u00c8 possibile includere anche i PFAS a catena pi\u00f9 lunga. Questo metodo pu\u00f2 essere applicato all\u2019analisi dell\u2019acqua sia potabile che non potabile e offre un set-up ideale, garantendo un\u2019elevata produttivit\u00e0 ai laboratori interessati ad aggiungere composti a catena ultra-corta a un\u2019analisi dei PFAS esistente.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Analisi completa dei PFAS a catena ultra-corta (C2,C3), dei PFAS convenzionali e dei composti alternativi.<\/strong><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.png\" alt=\"Ultrashort- Through Long-Chain and Alternative PFAS on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11CL-PF3OUdS)<\/td><td class=\"oth\">1.25<\/td><td class=\"oth\">400<\/td><td class=\"oth\">630.78<\/td><td class=\"oth\">450.80<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)<\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">400<\/td><td class=\"oth\">530.78<\/td><td class=\"oth\">350.85<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">400<\/td><td class=\"oth\">498.84<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanesulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">400<\/td><td class=\"oth\">398.90<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanesulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanesulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">1.64<\/td><td class=\"oth\">400<\/td><td class=\"oth\">298.97<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoropropanesulfonic acid (PFPrS)<\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">400<\/td><td class=\"oth\">248.97<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">Perfluoroethanesulfonic acid (PFEtS)<\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">400<\/td><td class=\"oth\">198.98<\/td><td class=\"oth\">79.92<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hexafluoropropylene oxide dimer acid (HFPO-DA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13252-13-6\/Hexafluoropropylene oxide dimer acid\" target=\"_blank\" rel=\"noopener\">Hexafluoropropylene oxide dimer acid (HFPO-DA)<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">284.97<\/td><td class=\"oth\">168.92<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">400<\/td><td class=\"oth\">412.90<\/td><td class=\"oth\">368.91<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/919005-14-4\/Ammonium 4,8-dioxa-3H-perfluorononanoate\" target=\"_blank\" rel=\"noopener\">Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)<\/a><\/td><td class=\"oth\">2.15<\/td><td class=\"oth\">400<\/td><td class=\"oth\">376.90<\/td><td class=\"oth\">250.93<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">2.36<\/td><td class=\"oth\">400<\/td><td class=\"oth\">312.97<\/td><td class=\"oth\">268.90<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanoic acid (PFBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-22-4\/Perfluorobutanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanoic acid (PFBA)<\/a><\/td><td class=\"oth\">2.76<\/td><td class=\"oth\">400<\/td><td class=\"oth\">212.97<\/td><td class=\"oth\">168.97<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Perfluoropropionic acid (PFPrA)<\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">163.03<\/td><td class=\"oth\">119.01<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoroacetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoroacetic acid (TFA)<\/a><\/td><td class=\"oth\">3.77<\/td><td class=\"oth\">400<\/td><td class=\"oth\">113.03<\/td><td class=\"oth\">69.01<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>400  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 10 mM ammonium formate, 0.05% formic acid   <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>60:40 Acetonitrile:methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>To achieve more stable and robust performance with Polar X columns, the recommended LC conditions have changed from those that were used here. The new recommended conditions are an aqueous mobile phase (mobile phase A) consisting of 5 mM or 10 mM ammonium formate with 0.1% formic acid in water and an organic mobile phase (mobile phase B) of 100% acetonitrile. Higher ammonium formate concentrations will result in reduced analyte retention. Therefore, laboratories can optimize retention by adjusting both the ammonium formate concentration and the flow rate to best meet their analytical requirements. In addition, it is recommended that labs evaluate different isocratic conditions by varying the amount of mobile phase B between 70% and 85% acetonitrile. This approach can help labs identify the most suitable separation conditions for their specific target analytes and sample matrices of interest.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9311A52<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>PFAS<\/li><li>PFAS-library<\/li><li>ultrashort-chain<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Innovativa fase stazionaria per un\u2019analisi completa dei PFAS: composti a catena ultra-corta (C2, C3), alternativi e tradizionali<\/p>\n","protected":false},"author":11,"featured_media":6719,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":"","_ppma_block_editor_authors":""},"categories":[788],"tags":[2782],"industries-application":[2255,2253,2257,2766],"post-badge":[],"resource-type":[2768],"product-library":[2535,2517],"resource-technique":[2334,2364],"ppma_author":[414],"class_list":["post-43488","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articoli","tag-pfas","industries-application-acque-potabili-acque-reflue","industries-application-ambientale","industries-application-analisi-dei-pfas","industries-application-semivolatiles","resource-type-featured-application","product-library-colonne-lc","product-library-prodotti-per-cromatografia-liquida","resource-technique-cromatografia-liquida-lc","resource-technique-ms-ms-it"],"acf":[],"taxonomy_info":{"category":[{"value":788,"label":"Articoli"}],"post_tag":[{"value":2782,"label":"PFAS"}],"industries-application":[{"value":2255,"label":"Acque Potabili e Acque Reflue"},{"value":2253,"label":"Ambientale"},{"value":2257,"label":"Analisi dei PFAS"},{"value":2766,"label":"Semivolatiles"}],"resource-type":[{"value":2768,"label":"Featured Application"}],"product-library":[{"value":2535,"label":"Colonne LC"},{"value":2517,"label":"Prodotti per Cromatografia Liquida"}],"resource-technique":[{"value":2334,"label":"Cromatografia Liquida (LC)"},{"value":2364,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-EVFA3197-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/it\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":788,"name":"Articoli","slug":"articoli","term_group":0,"term_taxonomy_id":788,"taxonomy":"category","description":"","parent":0,"count":511,"filter":"raw","cat_ID":788,"category_count":511,"category_description":"","cat_name":"Articoli","category_nicename":"articoli","category_parent":0}],"tag_info":[{"term_id":2782,"name":"PFAS","slug":"pfas","term_group":0,"term_taxonomy_id":2782,"taxonomy":"post_tag","description":"","parent":0,"count":124,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"author_category":"1","first_name":"Restek","last_name":"Corporation","user_url":"https:\/\/www.linkedin.com\/company\/restek-corporation\/posts\/?feedView=all","job_title":"Chromatography Products and Solutions","description":"Restek is a leading provider of chromatography columns, accessories, and certified reference materials. 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