{"id":43490,"date":"2021-09-24T00:00:00","date_gmt":"2021-09-24T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/fast-8-minute-method-for-direct-injection-of-pfas-in-non-potable-water\/"},"modified":"2026-08-05T19:38:43","modified_gmt":"2026-08-05T19:38:43","slug":"fast-8-minute-method-for-direct-injection-of-pfas-in-non-potable-water","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/articles\/evfa3693\/fast-8-minute-method-for-direct-injection-of-pfas-in-non-potable-water","title":{"rendered":"Fast, 8-Minute Method for Direct Injection of PFAS in Non-Potable Water"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Analyze EPA Method 8327 PFAS 3x faster than method conditions.<\/li>\n\n\n\n<li>Direct injection means no time-consuming sample preparation steps.<\/li>\n\n\n\n<li>Force C18 FPP column provides extra retention and improved peak shape for PFBA.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">While methods for analyzing per- and polyfluoroalkyl substances (PFAS) in drinking water typically use SPE sample preparation (styrene divinylbenzene or weak anion exchange), non-potable waters are most often analyzed using direct injection. EPA Method 8327, for example, is a validated test method used to measure a group of 24 PFAS compounds in groundwater, surface water, and wastewater samples using LC-MS\/MS. Direct injection can be advantageous because time-consuming sample preparation and its associated costs and variability can be avoided, but the typical 21-minute analysis time limits sample throughput. To provide a faster approach for direct injection of PFAS, we have established a chromatographic method for the comprehensive analysis of the 24 target and 19 surrogate PFAS in EPA 8327 with a total cycle time of just 8 minutes. This method also provides proper detection sensitivity (10 ppt before dilution) for all analytes.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Using a 1:1 dilution with methanol (0.1% acetic acid) and the conditions shown below, all compounds were successfully analyzed on a Force C18 (50 mm x 2.1 mm, 3 \u00b5m) fully porous particle (FPP) column. The high surface area and carbon load of the FPP column provides increased chromatographic retention compared to a superficially porous particle (SPP) column, which can be advantageous when PFBA is a target analyte. As an early eluting compound, PFBA can be difficult to chromatograph, especially if there is a mismatch between the sample diluent and the initial mobile phase composition. When this occurs, PFBA peak shape can be very poor, and fronting or peak splitting may be observed. An effective way to address this is to increase the retention time of PFBA by using a Force C18 column. As shown below, labs running direct injection of PFAS can obtain excellent resolution of EPA Method 8327 compounds in a third of the time using a Force C18 column. In addition, labs dealing with poor retention and peak shape for PFBA on a superficially porous particle (SPP) column will benefit from using a Force C18 column under the conditions established here.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0578.png\" alt=\"PFAS by Direct Injection on Force C18 (3 \u00b5m) (EPA Method 8327)\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0578<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanoic acid (PFBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-22-4\/Perfluorobutanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanoic acid (PFBA)<\/a><\/td><td class=\"oth\">1.56<\/td><td class=\"oth\">80<\/td><td class=\"oth\">231.1<\/td><td class=\"oth\">169.1<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Perfluoro-n-[<sup>13<\/sup>C<sub>4<\/sub>]butanoic acid (M4PFBA)<\/td><td class=\"oth\">1.56<\/td><td class=\"oth\">40<\/td><td class=\"oth\">217.0<\/td><td class=\"oth\">172.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoropentanoic acid (PFPeA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2706-90-3\/Perfluoropentanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoropentanoic acid (PFPeA)<\/a><\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">80<\/td><td class=\"oth\">263.0<\/td><td class=\"oth\">219.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\">Perfluoro-n-[<sup>13<\/sup>C<sub>5<\/sub>]pentanoic acid (M5PFPeA)<\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">40<\/td><td class=\"oth\">268.0<\/td><td class=\"oth\">223.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutane sulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutane sulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">2.90<\/td><td class=\"oth\">80<\/td><td class=\"oth\">299.1<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoro-1-[2,3,4-<sup>13<\/sup>C<sub>3<\/sub>]butyl sulfonic acid (M3PFBS)<\/td><td class=\"oth\">2.90<\/td><td class=\"oth\">40<\/td><td class=\"oth\">302.0<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1H, 1H, 2H, 2H-perfluorohexane sulfonic acid (4:2 FTS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/757124-72-4\/1H, 1H, 2H, 2H-perfluorohexane sulfonic acid\" target=\"_blank\" rel=\"noopener\">1H, 1H, 2H, 2H-perfluorohexane sulfonic acid (4:2 FTS)<\/a><\/td><td class=\"oth\">3.43<\/td><td class=\"oth\">80<\/td><td class=\"oth\">327.1<\/td><td class=\"oth\">307.1<\/td><td class=\"oth\">80.8<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">1H, 1H, 2H, 2H-perfluoro-1-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]hexyl sulfonic acid (M2-4:2 FTS)<\/td><td class=\"oth\">3.43<\/td><td class=\"oth\">40<\/td><td class=\"oth\">329.0<\/td><td class=\"oth\">309.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">3.49<\/td><td class=\"oth\">80<\/td><td class=\"oth\">313.1<\/td><td class=\"oth\">269.1<\/td><td class=\"oth\">119.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2,3,4,6-<sup>13<\/sup>C<sub>5<\/sub>]hexanoic acid (M5PFHxA)<\/td><td class=\"oth\">3.49<\/td><td class=\"oth\">40<\/td><td class=\"oth\">318.0<\/td><td class=\"oth\">273.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoropentane sulfonic acid (PFPeS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2706-91-4\/Perfluoropentane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluoropentane sulfonic acid (PFPeS)<\/a><\/td><td class=\"oth\">3.58<\/td><td class=\"oth\">80<\/td><td class=\"oth\">349.1<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroheptanoic acid (PFHpA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-85-9\/Perfluoroheptanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroheptanoic acid (PFHpA)<\/a><\/td><td class=\"oth\">4.04<\/td><td class=\"oth\">80<\/td><td class=\"oth\">363.2<\/td><td class=\"oth\">319.1<\/td><td class=\"oth\">169.1<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2,3,4-<sup>13<\/sup>C<sub>4<\/sub>]heptanoic acid (M4PFHpA)\t<\/td><td class=\"oth\">4.04<\/td><td class=\"oth\">40<\/td><td class=\"oth\">367.0<\/td><td class=\"oth\">322.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexane sulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexane sulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">4.07<\/td><td class=\"oth\">80<\/td><td class=\"oth\">399.1<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\">Perfluoro-1-[1,2,3-<sup>13<\/sup>C<sub>3<\/sub>]hexyl sulfonic acid (M3PFHxS)<\/td><td class=\"oth\">4.07<\/td><td class=\"oth\">40<\/td><td class=\"oth\">402.0<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1H, 1H, 2H, 2H-perfluorooctane sulfonic acid (6:2 FTS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/27619-97-2\/1H, 1H, 2H, 2H-perfluorooctane sulfonic acid\" target=\"_blank\" rel=\"noopener\">1H, 1H, 2H, 2H-perfluorooctane sulfonic acid (6:2 FTS)<\/a><\/td><td class=\"oth\">4.46<\/td><td class=\"oth\">80<\/td><td class=\"oth\">427.2<\/td><td class=\"oth\">407.2<\/td><td class=\"oth\">80.7<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\">1H, 1H, 2H, 2H-perfluoro-1-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]octyl sulfonic acid (M2-6:2 FTS)<\/td><td class=\"oth\">4.46<\/td><td class=\"oth\">40<\/td><td class=\"oth\">429.0<\/td><td class=\"oth\">409.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">4.48<\/td><td class=\"oth\">80<\/td><td class=\"oth\">412.9<\/td><td class=\"oth\">369.0<\/td><td class=\"oth\">169.0<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\">Perfluoro-n-[<sup>13<\/sup>C<sub>8<\/sub>]octanoic acid (M8PFOA)<\/td><td class=\"oth\">4.48<\/td><td class=\"oth\">40<\/td><td class=\"oth\">421.0<\/td><td class=\"oth\">376.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroheptane sulfonic acid (PFHpS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-92-8\/Perfluoroheptane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroheptane sulfonic acid (PFHpS)<\/a><\/td><td class=\"oth\">4.49<\/td><td class=\"oth\">80<\/td><td class=\"oth\">449.2<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononanoic acid (PFNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-95-1\/Perfluorononanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononanoic acid (PFNA)<\/a><\/td><td class=\"oth\">4.84<\/td><td class=\"oth\">80<\/td><td class=\"oth\">463.2<\/td><td class=\"oth\">419.2<\/td><td class=\"oth\">219.1<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\">Perfluoro-n-[<sup>13<\/sup>C<sub>9<\/sub>]nonanoic acid (M9PFNA)<\/td><td class=\"oth\">4.84<\/td><td class=\"oth\">40<\/td><td class=\"oth\">472.0<\/td><td class=\"oth\">427.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctane sulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctane sulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">4.84<\/td><td class=\"oth\">80<\/td><td class=\"oth\">499.2<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\">Perfluoro-1-[<sup>13<\/sup>C<sub>8<\/sub>]octyl sulfonic acid (M8PFOS)<\/td><td class=\"oth\">4.84<\/td><td class=\"oth\">40<\/td><td class=\"oth\">506.8<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorononane sulfonic acid (PFNS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68259-12-1\/Perfluorononane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorononane sulfonic acid (PFNS)<\/a><\/td><td class=\"oth\">5.14<\/td><td class=\"oth\">80<\/td><td class=\"oth\">549.2<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1H, 1H, 2H, 2H-perfluorodecane sulfonic acid (8:2 FTS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/39108-34-4\/1H, 1H, 2H, 2H-perfluorodecane sulfonic acid\" target=\"_blank\" rel=\"noopener\">1H, 1H, 2H, 2H-perfluorodecane sulfonic acid (8:2 FTS)<\/a><\/td><td class=\"oth\">5.15<\/td><td class=\"oth\">80<\/td><td class=\"oth\">527.2<\/td><td class=\"oth\">507.2<\/td><td class=\"oth\">80.8<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\">1H, 1H, 2H, 2H-perfluoro-1-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]decyl sulfonic acid (M2-8:2 FTS)<\/td><td class=\"oth\">5.15<\/td><td class=\"oth\">40<\/td><td class=\"oth\">529.0<\/td><td class=\"oth\">509.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">28.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecanoic acid (PFDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-76-2\/Perfluorodecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecanoic acid (PFDA)<\/a><\/td><td class=\"oth\">5.15<\/td><td class=\"oth\">80<\/td><td class=\"oth\">513.2<\/td><td class=\"oth\">469.2<\/td><td class=\"oth\">219.1<\/td><\/tr>\n<tr><td class=\"num\">29.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2,3,4,5,6-<sup>13<\/sup>C<sub>6<\/sub>]decanoic acid (M6PFDA)<\/td><td class=\"oth\">5.15<\/td><td class=\"oth\">40<\/td><td class=\"oth\">518.9<\/td><td class=\"oth\">473.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">30.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-methylperfluoro-1-octanesulfonamidoacetic acid (N-MeFOSAA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2355-31-9\/N-methylperfluoro-1-octanesulfonamidoacetic acid\" target=\"_blank\" rel=\"noopener\">N-methylperfluoro-1-octanesulfonamidoacetic acid (N-MeFOSAA)<\/a><\/td><td class=\"oth\">5.28<\/td><td class=\"oth\">80<\/td><td class=\"oth\">570.2<\/td><td class=\"oth\">419.2<\/td><td class=\"oth\">483.2<\/td><\/tr>\n<tr><td class=\"num\">31.<\/td><td class=\"cmpd\">N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)<\/td><td class=\"oth\">5.28<\/td><td class=\"oth\">40<\/td><td class=\"oth\">572.9<\/td><td class=\"oth\">418.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">32.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorodecane sulfonic acid (PFDS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-77-3\/Perfluorodecane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorodecane sulfonic acid (PFDS)<\/a><\/td><td class=\"oth\">5.40<\/td><td class=\"oth\">80<\/td><td class=\"oth\">599.2<\/td><td class=\"oth\">80.0<\/td><td class=\"oth\">99.0<\/td><\/tr>\n<tr><td class=\"num\">33.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-ethylperfluoro-1-octanesulfonamidoacetic acid (N-EtFOSAA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2991-50-6\/N-ethylperfluoro-1-octanesulfonamidoacetic acid\" target=\"_blank\" rel=\"noopener\">N-ethylperfluoro-1-octanesulfonamidoacetic acid (N-EtFOSAA)<\/a><\/td><td class=\"oth\">5.42<\/td><td class=\"oth\">80<\/td><td class=\"oth\">584.2<\/td><td class=\"oth\">419.2<\/td><td class=\"oth\">483.1<\/td><\/tr>\n<tr><td class=\"num\">34.<\/td><td class=\"cmpd\">N-ethyl-d5-perfluoro-1-catanesulfonamidoacetic acid (d5-N-EtFOSAA)<\/td><td class=\"oth\">5.42<\/td><td class=\"oth\">40<\/td><td class=\"oth\">589.0<\/td><td class=\"oth\">419.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">35.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluoroundecanoic acid (PFUnA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2058-94-8\/Perfluoroundecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluoroundecanoic acid (PFUnA)<\/a><\/td><td class=\"oth\">5.42<\/td><td class=\"oth\">80<\/td><td class=\"oth\">563.2<\/td><td class=\"oth\">519.2<\/td><td class=\"oth\">269.1<\/td><\/tr>\n<tr><td class=\"num\">36.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2,3,4,5,6,7-<sup>13<\/sup>C<sub>7<\/sub>]undecanoic acid (M7PFUnA)<\/td><td class=\"oth\">5.42<\/td><td class=\"oth\">40<\/td><td class=\"oth\">569.9<\/td><td class=\"oth\">524.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">37.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctane sulfonic acid (FOSA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctane sulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctane sulfonic acid (FOSA)<\/a><\/td><td class=\"oth\">5.49<\/td><td class=\"oth\">80<\/td><td class=\"oth\">498.2<\/td><td class=\"oth\">78.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">38.<\/td><td class=\"cmpd\">Perfluoro-1-[<sup>13<\/sup>C<sub>8<\/sub>]octanesulfonamide (M8FOSA)<\/td><td class=\"oth\">5.49<\/td><td class=\"oth\">40<\/td><td class=\"oth\">505.9<\/td><td class=\"oth\">78.0<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">39.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorododecanoic acid (PFDoA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-55-1\/Perfluorododecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorododecanoic acid (PFDoA)<\/a><\/td><td class=\"oth\">5.65<\/td><td class=\"oth\">80<\/td><td class=\"oth\">613.2<\/td><td class=\"oth\">569.2<\/td><td class=\"oth\">169.1<\/td><\/tr>\n<tr><td class=\"num\">40.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2,-<sup>13<\/sup>C<sub>2<\/sub>]dodecanoic acid (M2PFDoA)<\/td><td class=\"oth\">5.65<\/td><td class=\"oth\">40<\/td><td class=\"oth\">614.8<\/td><td class=\"oth\">569.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">41.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorotridecanoic acid (PFTriA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72629-94-8\/Perfluorotridecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorotridecanoic acid (PFTriA)<\/a><\/td><td class=\"oth\">5.86<\/td><td class=\"oth\">80<\/td><td class=\"oth\">663.2<\/td><td class=\"oth\">619.2<\/td><td class=\"oth\">169.1<\/td><\/tr>\n<tr><td class=\"num\">42.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorotetradecanoic acid (PFTreA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/376-06-7\/Perfluorotetradecanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorotetradecanoic acid (PFTreA)<\/a><\/td><td class=\"oth\">6.05<\/td><td class=\"oth\">80<\/td><td class=\"oth\">713.2<\/td><td class=\"oth\">669.2<\/td><td class=\"oth\">169.1<\/td><\/tr>\n<tr><td class=\"num\">43.<\/td><td class=\"cmpd\">Perfluoro-n-[1,2-<sup>13<\/sup>C<sub>2<\/sub>]tetradecanoic acid (M2PFTreA)<\/td><td class=\"oth\">6.05<\/td><td class=\"oth\">40<\/td><td class=\"oth\">714.8<\/td><td class=\"oth\">669.7<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634352\" rel=\"noopener\">cat.# 9634352<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>3 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol, 0.1% acetic acid<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>80  ppt (40 ppt for surrogate or isotopically-labelled PFAS)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 5 mM ammonium acetate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>6.00<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>6.51<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>8.50<\/td><td>0.4<\/td><td>80<\/td><td>20<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>A PFAS delay column (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/27854\" rel=\"noopener\">cat.# 27854<\/a>) was installed before the injector.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9634352<\/li><li>27854<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>8327<\/li><li>PFAS<\/li><li>PFAS-library<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0578.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Prodotti Menzionati<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9634352\">                                N\u00b0 Catalogo 9634352                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonna LC Force C18, 3 \u00b5m, 50 x 2,1 mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9634352\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/27854\">                                N\u00b0 Catalogo 27854                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">PFAS Delay Column, 5 \u00b5m, 50 x 2,1 mm, colonna HPLC<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/27854\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div class=\"wp-block-group hub-fullwidth-container has-theme-palette-9-background-color has-background\" style=\"margin-top:0px;margin-bottom:0px;padding-top:var(--wp--preset--spacing--50);padding-bottom:var(--wp--preset--spacing--50)\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\"><style>.kadence-column99903_13002f-36 > .kt-inside-inner-col{padding-top:45px;padding-right:0px;padding-bottom:45px;padding-left:0px;}.kadence-column99903_13002f-36 > .kt-inside-inner-col,.kadence-column99903_13002f-36 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column99903_13002f-36 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column99903_13002f-36 > .kt-inside-inner-col{flex-direction:column;}.kadence-column99903_13002f-36 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column99903_13002f-36 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column99903_13002f-36{position:relative;}@media all and (max-width: 1024px){.kadence-column99903_13002f-36 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column99903_13002f-36 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column99903_13002f-36 hub-guttered-centered-container\"><div class=\"kt-inside-inner-col\"><style>.kb-row-layout-id99903_d4b38f-90 > .kt-row-column-wrap{align-content:start;}:where(.kb-row-layout-id99903_d4b38f-90 > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}.kb-row-layout-id99903_d4b38f-90 > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:20px;padding-right:20px;padding-bottom:20px;padding-left:20px;grid-template-columns:minmax(0, 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.kt-inside-inner-col,.kadence-column99903_e9827f-3e > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column99903_e9827f-3e > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column99903_e9827f-3e > .kt-inside-inner-col{flex-direction:column;}.kadence-column99903_e9827f-3e > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column99903_e9827f-3e > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column99903_e9827f-3e{position:relative;}@media all and (max-width: 1024px){.kadence-column99903_e9827f-3e > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column99903_e9827f-3e > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column99903_e9827f-3e\"><div class=\"kt-inside-inner-col\"><div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-gnlp4243-pfas-hub-contact-an-expert-leaf-graphic-1024x1024.png\" alt=\"Icon depicting customer service person.\" class=\"wp-image-95744\" style=\"width:100px\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-gnlp4243-pfas-hub-contact-an-expert-leaf-graphic-1024x1024.png 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-gnlp4243-pfas-hub-contact-an-expert-leaf-graphic-300x300.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-gnlp4243-pfas-hub-contact-an-expert-leaf-graphic-150x150.png 150w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-gnlp4243-pfas-hub-contact-an-expert-leaf-graphic-768x768.png 768w, 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mark.kt-highlight, .wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b[data-kb-block=\"kb-adv-heading99903_c05470-6b\"] mark.kt-highlight{font-style:normal;-webkit-text-fill-color:initial !important;-webkit-background-clip:initial !important;background-clip:initial !important;color:#f76a0c;-webkit-box-decoration-break:clone;box-decoration-break:clone;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b img.kb-inline-image, .wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b[data-kb-block=\"kb-adv-heading99903_c05470-6b\"] img.kb-inline-image{width:150px;vertical-align:baseline;}@media all and (max-width: 1024px){.wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b, .wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b[data-kb-block=\"kb-adv-heading99903_c05470-6b\"]{font-size:40px;}}@media all and (max-width: 767px){.wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b, .wp-block-kadence-advancedheading.kt-adv-heading99903_c05470-6b[data-kb-block=\"kb-adv-heading99903_c05470-6b\"]{font-size:36px;}}<\/style><h2 class=\"kt-adv-heading99903_c05470-6b wp-block-kadence-advancedheading\" data-kb-block=\"kb-adv-heading99903_c05470-6b\"><span class=\"kb-adv-text-inner\">Need Help with Your PFAS Analysis?<\/span><\/h2>\n\n\n<p class=\"has-text-align-center has-17-font-size has-theme-palette-3-color has-text-color has-link-color wp-elements-7b0078e0cfc8a83ec72b6ed06071decd wp-block-paragraph\" style=\"padding-right:var(--wp--preset--spacing--50);padding-left:var(--wp--preset--spacing--50);font-size:23px\">From method selection to product recommendations, our PFAS experts are here to help you navigate complex workflows and achieve accurate, reliable results.<\/p>\n\n\n<style>.wp-block-kadence-advancedbtn.kb-btns99903_cbdd3e-ae{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns99903_cbdd3e-ae .kt-button{font-weight:normal;font-style:normal;}.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button{color:#555555;border-color:#555555;}.wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns99903_cbdd3e-ae .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kb-buttons-wrap kb-btns99903_cbdd3e-ae\"><style>ul.menu .wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button{width:initial;}.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button{color:var(--global-palette9, #ffffff);background:#61b522;font-size:var(--global-kb-font-size-md, 1.25rem);border-top-left-radius:10px;border-top-right-radius:10px;border-bottom-right-radius:10px;border-bottom-left-radius:10px;border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}.kb-btn99903_1b0338-26.kb-button .kb-svg-icon-wrap{color:var(--global-palette9, #ffffff);}.kb-btn99903_1b0338-26.kb-button:hover .kb-svg-icon-wrap, .kb-btn99903_1b0338-26.kb-button:focus .kb-svg-icon-wrap{color:#61b522;}.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:hover, .wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:focus{color:#61b522;background:var(--global-palette9, #ffffff);border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}@media all and (max-width: 1024px){.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button{border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}}@media all and (max-width: 1024px){.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:hover, .wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:focus{border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}}@media all and (max-width: 767px){.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button{border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}.wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:hover, .wp-block-kadence-advancedbtn .kb-btn99903_1b0338-26.kb-button:focus{border-top:2px solid #61b522;border-right:2px solid #61b522;border-bottom:2px solid #61b522;border-left:2px solid #61b522;}}<\/style><a class=\"kb-button kt-button button kb-btn99903_1b0338-26 kt-btn-size-standard kt-btn-width-type-auto kb-btn-global-outline kt-btn-has-text-true kt-btn-has-svg-true wp-block-kadence-singlebtn\" href=\"https:\/\/explore.restek.com\/PFAS\" target=\"_blank\" rel=\"noopener\"><span class=\"kt-btn-inner-text\"><strong>Contact a PFAS Expert<\/strong><\/span><span class=\"kb-svg-icon-wrap kb-svg-icon-fe_arrowRight kt-btn-icon-side-right\"><svg viewBox=\"0 0 24 24\"  fill=\"none\" stroke=\"currentColor\" stroke-width=\"2\" stroke-linecap=\"round\" stroke-linejoin=\"round\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"  aria-hidden=\"true\"><line x1=\"5\" y1=\"12\" x2=\"19\" y2=\"12\"\/><polyline points=\"12 5 19 12 12 19\"\/><\/svg><\/span><\/a><\/div>\n<\/div><\/div>\n\n<\/div><\/div><\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The direct injection of PFAS method established here provides excellent results for EPA Method 8327 compounds in just 8 minutes. 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