{"id":43623,"date":"2020-10-29T14:30:00","date_gmt":"2020-10-29T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/una-sola-analisi-una-sola-colonna-meno-di-9-minuti-per-oltre-60-antibiotici-multiclasse\/"},"modified":"2025-11-26T17:42:04","modified_gmt":"2025-11-26T17:42:04","slug":"una-sola-analisi-una-sola-colonna-meno-di-9-minuti-per-oltre-60-antibiotici-multiclasse","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/articoli\/fsss2276-it\/una-sola-analisi-una-sola-colonna-meno-di-9-minuti-per-oltre-60-antibiotici-multiclasse","title":{"rendered":"Una sola analisi, una sola colonna, meno di 9 minuti per oltre 60 antibiotici multiclasse"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Separazione dei picchi altamente efficiente e tempi di analisi rapidi.<\/li>\n\n\n\n<li>Applicabilit\u00e0 universale per l\u2019analisi residuale degli antibiotici, con possibilit\u00e0 di ottimizzare l\u2019analisi di singole classi di composti ai fini della quantificazione:\n<ul class=\"wp-block-list\">\n<li>Macrolidi, lincosamidi, e streptogramine (Figura 1)<\/li>\n\n\n\n<li>Amfenicoli e tetracicline (Figura 2)<\/li>\n\n\n\n<li>Chinoloni (Figura 3)<\/li>\n\n\n\n<li>Penicilline, cefalosporine e tetracicline (Figura 4)<\/li>\n\n\n\n<li>Sulfamidici (Figura 5) (Per gli ionofori \u00e8 stata utilizzata la Raptor bifenilica. Figura 6)<br><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<p>L&#8217;uso di antibiotici sugli animali destinati alla produzione alimentare \u00e8 un problema di salute e sicurezza pubblica in quanto potrebbero svilupparsi batteri farmacoresistenti. Molti paesi dell&#8217;Unione europea e il Canada hanno vietato l&#8217;uso di questi farmaci per scopi non terapeutici, e gli Stati Uniti stanno implementando una politica volta a ridurre l&#8217;uso di antibiotici di rilievo clinico come promotori della crescita. Per regolamentare l&#8217;uso corretto degli antibiotici veterinari, la statunitense FDA ha fissato i limiti massimi dei residui (LMR) per diversi tessuti animali e prodotti alimentari (21 CFR Part 556). Per operare in conformit\u00e0 a queste disposizioni \u00e8 necessario impiegare un metodo analitico sensibile, efficiente e affidabile per diverse classi di antibiotici, e la colonna LC Raptor C18 \u00e8 la scelta ideale.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0506.png\" alt=\"Multiclass Veterinary Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0506<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desacetyl cephapirin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/38115-21-8\/Desacetyl cephapirin\" target=\"_blank\" rel=\"noopener\">Desacetyl cephapirin<\/a><\/td><td class=\"oth\">0.70<\/td><td class=\"oth\">150<\/td><td class=\"oth\">382.03<\/td><td class=\"oth\">111.92<\/td><td class=\"oth\">124.21<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfanilamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-74-1\/Sulfanilamide\" target=\"_blank\" rel=\"noopener\">Sulfanilamide<\/a><\/td><td class=\"oth\">0.85<\/td><td class=\"oth\">200<\/td><td class=\"oth\">172.98<\/td><td class=\"oth\">93.07<\/td><td class=\"oth\">75.23<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amoxicillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26787-78-0\/Amoxicillin\" target=\"_blank\" rel=\"noopener\">Amoxicillin<\/a><\/td><td class=\"oth\">0.92<\/td><td class=\"oth\">100<\/td><td class=\"oth\">366.24<\/td><td class=\"oth\">349.10<\/td><td class=\"oth\">208.07<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cephapirin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21593-23-7\/Cephapirin\" target=\"_blank\" rel=\"noopener\">Cephapirin<\/a><\/td><td class=\"oth\">1.36<\/td><td class=\"oth\">50<\/td><td class=\"oth\">424.17<\/td><td class=\"oth\">292.08<\/td><td class=\"oth\">124.14<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tildipirosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/328898-40-4\/Tildipirosin\" target=\"_blank\" rel=\"noopener\">Tildipirosin<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">200<\/td><td class=\"oth\">734.59<\/td><td class=\"oth\">561.45<\/td><td class=\"oth\">204.15<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desfuroyl ceftiofur cysteine disulfide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/158039-15-7\/Desfuroyl ceftiofur cysteine disulfide\" target=\"_blank\" rel=\"noopener\">Desfuroyl ceftiofur cysteine disulfide<\/a><\/td><td class=\"oth\">1.40<\/td><td class=\"oth\">300<\/td><td class=\"oth\">549.16<\/td><td class=\"oth\">183.02<\/td><td class=\"oth\">126.00<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lincomycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/154-21-2\/Lincomycin\" target=\"_blank\" rel=\"noopener\">Lincomycin<\/a><\/td><td class=\"oth\">1.50<\/td><td class=\"oth\">50<\/td><td class=\"oth\">407.32<\/td><td class=\"oth\">359.23<\/td><td class=\"oth\">389.28<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadiazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68-35-9\/Sulfadiazine\" target=\"_blank\" rel=\"noopener\">Sulfadiazine<\/a><\/td><td class=\"oth\">1.57<\/td><td class=\"oth\">20<\/td><td class=\"oth\">251.18<\/td><td class=\"oth\">156.04<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefquinome\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/84957-30-2\/Cefquinome\" target=\"_blank\" rel=\"noopener\">Cefquinome<\/a><\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">200<\/td><td class=\"oth\">529.19<\/td><td class=\"oth\">134.10<\/td><td class=\"oth\">125.12<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ampicillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/69-52-3\/Ampicillin\" target=\"_blank\" rel=\"noopener\">Ampicillin<\/a><\/td><td class=\"oth\">1.78<\/td><td class=\"oth\">50<\/td><td class=\"oth\">350.19<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">160.06<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfathiazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-14-0\/Sulfathiazole\" target=\"_blank\" rel=\"noopener\">Sulfathiazole<\/a><\/td><td class=\"oth\">1.79<\/td><td class=\"oth\">10<\/td><td class=\"oth\">256.16<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Marbofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/115550-35-1\/Marbofloxacin\" target=\"_blank\" rel=\"noopener\">Marbofloxacin<\/a><\/td><td class=\"oth\">1.81<\/td><td class=\"oth\">10<\/td><td class=\"oth\">363.20<\/td><td class=\"oth\">72.11<\/td><td class=\"oth\">320.10<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefalexin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23325-78-2\/Cefalexin\" target=\"_blank\" rel=\"noopener\">Cefalexin<\/a><\/td><td class=\"oth\">1.82<\/td><td class=\"oth\">100<\/td><td class=\"oth\">348.10<\/td><td class=\"oth\">158.05<\/td><td class=\"oth\">174.05<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfapyridine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-83-2\/Sulfapyridine\" target=\"_blank\" rel=\"noopener\">Sulfapyridine<\/a><\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">10<\/td><td class=\"oth\">250.13<\/td><td class=\"oth\">156.10<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norfloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70458-96-7\/Norfloxacin\" target=\"_blank\" rel=\"noopener\">Norfloxacin<\/a><\/td><td class=\"oth\">1.96<\/td><td class=\"oth\">20<\/td><td class=\"oth\">320.23<\/td><td class=\"oth\">276.20<\/td><td class=\"oth\">233.13<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/82419-36-1\/Ofloxacin\" target=\"_blank\" rel=\"noopener\">Ofloxacin<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">10<\/td><td class=\"oth\">362.21<\/td><td class=\"oth\">318.20<\/td><td class=\"oth\">261.15<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamerazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-79-7\/Sulfamerazine\" target=\"_blank\" rel=\"noopener\">Sulfamerazine<\/a><\/td><td class=\"oth\">2.00<\/td><td class=\"oth\">20<\/td><td class=\"oth\">265.08<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefalonium\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5575-21-3\/Cefalonium\" target=\"_blank\" rel=\"noopener\">Cefalonium<\/a><\/td><td class=\"oth\">2.01<\/td><td class=\"oth\">100<\/td><td class=\"oth\">459.16<\/td><td class=\"oth\">337.03<\/td><td class=\"oth\">123.10<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxytetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-57-2\/Oxytetracycline\" target=\"_blank\" rel=\"noopener\">Oxytetracycline<\/a><\/td><td class=\"oth\">2.02<\/td><td class=\"oth\">25<\/td><td class=\"oth\">461.27<\/td><td class=\"oth\">426.15<\/td><td class=\"oth\">443.32<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ciprofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/85721-33-1\/Ciprofloxacin\" target=\"_blank\" rel=\"noopener\">Ciprofloxacin<\/a><\/td><td class=\"oth\">2.04<\/td><td class=\"oth\">20<\/td><td class=\"oth\">332.18<\/td><td class=\"oth\">288.22<\/td><td class=\"oth\">245.15<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefacetrile\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10206-21-0\/Cefacetrile\" target=\"_blank\" rel=\"noopener\">Cefacetrile<\/a><\/td><td class=\"oth\">2.09<\/td><td class=\"oth\">300<\/td><td class=\"oth\">362.07<\/td><td class=\"oth\">258.08<\/td><td class=\"oth\">178.01<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tulathromycin A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/217500-96-4\/Tulathromycin A\" target=\"_blank\" rel=\"noopener\">Tulathromycin A<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">100<\/td><td class=\"oth\">806.65<\/td><td class=\"oth\">577.42<\/td><td class=\"oth\">420.31<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-54-8\/Tetracycline\" target=\"_blank\" rel=\"noopener\">Tetracycline<\/a><\/td><td class=\"oth\">2.21<\/td><td class=\"oth\">25<\/td><td class=\"oth\">445.28<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">427.32<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Danofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/112398-08-0\/Danofloxacin\" target=\"_blank\" rel=\"noopener\">Danofloxacin<\/a><\/td><td class=\"oth\">2.23<\/td><td class=\"oth\">20<\/td><td class=\"oth\">358.22<\/td><td class=\"oth\">340.16<\/td><td class=\"oth\">314.21<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Enrofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/93106-60-6\/Enrofloxacin\" target=\"_blank\" rel=\"noopener\">Enrofloxacin<\/a><\/td><td class=\"oth\">2.32<\/td><td class=\"oth\">10<\/td><td class=\"oth\">360.29<\/td><td class=\"oth\">316.22<\/td><td class=\"oth\">245.13<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Orbifloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/113617-63-3\/Orbifloxacin\" target=\"_blank\" rel=\"noopener\">Orbifloxacin<\/a><\/td><td class=\"oth\">2.35<\/td><td class=\"oth\">10<\/td><td class=\"oth\">396.22<\/td><td class=\"oth\">352.17<\/td><td class=\"oth\">226.12<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Thiamphenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/90-91-5\/Thiamphenicol\" target=\"_blank\" rel=\"noopener\">Thiamphenicol*<\/a><\/td><td class=\"oth\">2.38<\/td><td class=\"oth\">200<\/td><td class=\"oth\">354.16<\/td><td class=\"oth\">290.04<\/td><td class=\"oth\">184.98<\/td><\/tr>\n<tr><td class=\"num\">28.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-68-1\/Sulfamethazine\" target=\"_blank\" rel=\"noopener\">Sulfamethazine<\/a><\/td><td class=\"oth\">2.39<\/td><td class=\"oth\">10<\/td><td class=\"oth\">279.23<\/td><td class=\"oth\">186.08<\/td><td class=\"oth\">124.08<\/td><\/tr>\n<tr><td class=\"num\">29.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethizole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-82-1\/Sulfamethizole\" target=\"_blank\" rel=\"noopener\">Sulfamethizole<\/a><\/td><td class=\"oth\">2.52<\/td><td class=\"oth\">10<\/td><td class=\"oth\">271.17<\/td><td class=\"oth\">156.02<\/td><td class=\"oth\">108.02<\/td><\/tr>\n<tr><td class=\"num\">30.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethoxypyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-35-3\/Sulfamethoxypyridazine\" target=\"_blank\" rel=\"noopener\">Sulfamethoxypyridazine<\/a><\/td><td class=\"oth\">2.56<\/td><td class=\"oth\">10<\/td><td class=\"oth\">281.14<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">126.07<\/td><\/tr>\n<tr><td class=\"num\">31.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sarafloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98105-99-8\/Sarafloxacin\" target=\"_blank\" rel=\"noopener\">Sarafloxacin<\/a><\/td><td class=\"oth\">2.59<\/td><td class=\"oth\">10<\/td><td class=\"oth\">386.20<\/td><td class=\"oth\">342.20<\/td><td class=\"oth\">368.15<\/td><\/tr>\n<tr><td class=\"num\">32.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Difloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98106-17-3\/Difloxacin\" target=\"_blank\" rel=\"noopener\">Difloxacin<\/a><\/td><td class=\"oth\">2.65<\/td><td class=\"oth\">10<\/td><td class=\"oth\">400.23<\/td><td class=\"oth\">356.17<\/td><td class=\"oth\">299.13<\/td><\/tr>\n<tr><td class=\"num\">33.<\/td><td class=\"cmpd\">Cefazolin<\/td><td class=\"oth\">2.75<\/td><td class=\"oth\">100<\/td><td class=\"oth\">455.10<\/td><td class=\"oth\">323.06<\/td><td class=\"oth\">295.09<\/td><\/tr>\n<tr><td class=\"num\">34.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Spiramycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/8025-81-8\/Spiramycin\" target=\"_blank\" rel=\"noopener\">Spiramycin<\/a><\/td><td class=\"oth\">2.96<\/td><td class=\"oth\">200<\/td><td class=\"oth\">843.64<\/td><td class=\"oth\">540.36<\/td><td class=\"oth\">699.48<\/td><\/tr>\n<tr><td class=\"num\">35.<\/td><td class=\"cmpd\">Pirlimycin<\/td><td class=\"oth\">3.05<\/td><td class=\"oth\">20<\/td><td class=\"oth\">411.32<\/td><td class=\"oth\">363.18<\/td><td class=\"oth\">327.21<\/td><\/tr>\n<tr><td class=\"num\">36.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlortetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-62-5\/Chlortetracycline\" target=\"_blank\" rel=\"noopener\">Chlortetracycline<\/a><\/td><td class=\"oth\">3.08<\/td><td class=\"oth\">25<\/td><td class=\"oth\">479.27<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">371.06<\/td><\/tr>\n<tr><td class=\"num\">37.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfachlorpyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-32-0\/Sulfachlorpyridazine\" target=\"_blank\" rel=\"noopener\">Sulfachlorpyridazine<\/a><\/td><td class=\"oth\">3.12<\/td><td class=\"oth\">20<\/td><td class=\"oth\">285.05<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">38.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Gamithromycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/145435-72-9\/Gamithromycin\" target=\"_blank\" rel=\"noopener\">Gamithromycin<\/a><\/td><td class=\"oth\">3.28<\/td><td class=\"oth\">100<\/td><td class=\"oth\">777.63<\/td><td class=\"oth\">619.52<\/td><td class=\"oth\">601.45<\/td><\/tr>\n<tr><td class=\"num\">39.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2447-57-6\/Sulfadoxine\" target=\"_blank\" rel=\"noopener\">Sulfadoxine<\/a><\/td><td class=\"oth\">3.39<\/td><td class=\"oth\">10<\/td><td class=\"oth\">311.17<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">40.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/723-46-6\/Sulfamethoxazole\" target=\"_blank\" rel=\"noopener\">Sulfamethoxazole<\/a><\/td><td class=\"oth\">3.46<\/td><td class=\"oth\">20<\/td><td class=\"oth\">254.18<\/td><td class=\"oth\">155.98<\/td><td class=\"oth\">147.06<\/td><\/tr>\n<tr><td class=\"num\">41.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefoperazone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/62893-19-0\/Cefoperazone\" target=\"_blank\" rel=\"noopener\">Cefoperazone<\/a><\/td><td class=\"oth\">3.52<\/td><td class=\"oth\">100<\/td><td class=\"oth\">646.26<\/td><td class=\"oth\">143.07<\/td><td class=\"oth\">148.02<\/td><\/tr>\n<tr><td class=\"num\">42.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Florfenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73231-34-2\/Florfenicol\" target=\"_blank\" rel=\"noopener\">Florfenicol*<\/a><\/td><td class=\"oth\">3.55<\/td><td class=\"oth\">200<\/td><td class=\"oth\">356.10<\/td><td class=\"oth\">336.02<\/td><td class=\"oth\">184.98<\/td><\/tr>\n<tr><td class=\"num\">43.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaethoxypyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/963-14-4\/Sulfaethoxypyridazine\" target=\"_blank\" rel=\"noopener\">Sulfaethoxypyridazine<\/a><\/td><td class=\"oth\">3.56<\/td><td class=\"oth\">20<\/td><td class=\"oth\">295.17<\/td><td class=\"oth\">267.07<\/td><td class=\"oth\">156.03<\/td><\/tr>\n<tr><td class=\"num\">44.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tilmicosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108050-54-0\/Tilmicosin\" target=\"_blank\" rel=\"noopener\">Tilmicosin<\/a><\/td><td class=\"oth\">3.64<\/td><td class=\"oth\">100<\/td><td class=\"oth\">869.72<\/td><td class=\"oth\">696.50<\/td><td class=\"oth\">522.42<\/td><\/tr>\n<tr><td class=\"num\">45.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfisoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-69-5\/Sulfisoxazole\" target=\"_blank\" rel=\"noopener\">Sulfisoxazole<\/a><\/td><td class=\"oth\">3.76<\/td><td class=\"oth\">20<\/td><td class=\"oth\">268.14<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">113.10<\/td><\/tr>\n<tr><td class=\"num\">46.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxolinic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14698-29-4\/Oxolinic acid\" target=\"_blank\" rel=\"noopener\">Oxolinic acid<\/a><\/td><td class=\"oth\">3.94<\/td><td class=\"oth\">5<\/td><td class=\"oth\">262.10<\/td><td class=\"oth\">244.06<\/td><td class=\"oth\">215.96<\/td><\/tr>\n<tr><td class=\"num\">47.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chloramphenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-75-7\/Chloramphenicol\" target=\"_blank\" rel=\"noopener\">Chloramphenicol*<\/a><\/td><td class=\"oth\">4.00<\/td><td class=\"oth\">200<\/td><td class=\"oth\">321.16<\/td><td class=\"oth\">151.99<\/td><td class=\"oth\">257.04<\/td><\/tr>\n<tr><td class=\"num\">48.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ceftiofur\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80370-57-6\/Ceftiofur\" target=\"_blank\" rel=\"noopener\">Ceftiofur<\/a><\/td><td class=\"oth\">4.11<\/td><td class=\"oth\">50<\/td><td class=\"oth\">524.14<\/td><td class=\"oth\">241.08<\/td><td class=\"oth\">125.24<\/td><\/tr>\n<tr><td class=\"num\">49.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Erythromycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/114-07-8\/Erythromycin\" target=\"_blank\" rel=\"noopener\">Erythromycin<\/a><\/td><td class=\"oth\">4.31<\/td><td class=\"oth\">25<\/td><td class=\"oth\">734.64<\/td><td class=\"oth\">576.40<\/td><td class=\"oth\">558.38<\/td><\/tr>\n<tr><td class=\"num\">50.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadimethoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/122-11-2\/Sulfadimethoxine\" target=\"_blank\" rel=\"noopener\">Sulfadimethoxine<\/a><\/td><td class=\"oth\">4.36<\/td><td class=\"oth\">10<\/td><td class=\"oth\">311.17<\/td><td class=\"oth\">156.09<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">51.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaquinoxaline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-40-5\/Sulfaquinoxaline\" target=\"_blank\" rel=\"noopener\">Sulfaquinoxaline<\/a><\/td><td class=\"oth\">4.42<\/td><td class=\"oth\">20<\/td><td class=\"oth\">301.18<\/td><td class=\"oth\">156.04<\/td><td class=\"oth\">108.02<\/td><\/tr>\n<tr><td class=\"num\">52.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tylosin**\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1401-69-0\/Tylosin\" target=\"_blank\" rel=\"noopener\">Tylosin**<\/a><\/td><td class=\"oth\">4.67<\/td><td class=\"oth\">100<\/td><td class=\"oth\">916.62<\/td><td class=\"oth\">772.49<\/td><td class=\"oth\">598.36<\/td><\/tr>\n<tr><td class=\"num\">53.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Penicillin G\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-33-6\/Penicillin G\" target=\"_blank\" rel=\"noopener\">Penicillin G<\/a><\/td><td class=\"oth\">5.07<\/td><td class=\"oth\">100<\/td><td class=\"oth\">335.18<\/td><td class=\"oth\">176.07<\/td><td class=\"oth\">160.07<\/td><\/tr>\n<tr><td class=\"num\">54.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flumequine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/42835-25-6\/Flumequine\" target=\"_blank\" rel=\"noopener\">Flumequine<\/a><\/td><td class=\"oth\">5.34<\/td><td class=\"oth\">5<\/td><td class=\"oth\">262.15<\/td><td class=\"oth\">244.11<\/td><td class=\"oth\">202.03<\/td><\/tr>\n<tr><td class=\"num\">55.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Penicillin V\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/87-08-1\/Penicillin V\" target=\"_blank\" rel=\"noopener\">Penicillin V<\/a><\/td><td class=\"oth\">5.56<\/td><td class=\"oth\">100<\/td><td class=\"oth\">351.10<\/td><td class=\"oth\">160.06<\/td><td class=\"oth\">114.07<\/td><\/tr>\n<tr><td class=\"num\">56.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66-79-5\/Oxacillin\" target=\"_blank\" rel=\"noopener\">Oxacillin<\/a><\/td><td class=\"oth\">5.99<\/td><td class=\"oth\">100<\/td><td class=\"oth\">402.15<\/td><td class=\"oth\">160.05<\/td><td class=\"oth\">114.06<\/td><\/tr>\n<tr><td class=\"num\">57.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Virginiamycin M1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21411-53-0\/Virginiamycin M1\" target=\"_blank\" rel=\"noopener\">Virginiamycin M1<\/a><\/td><td class=\"oth\">6.13<\/td><td class=\"oth\">50<\/td><td class=\"oth\">526.43<\/td><td class=\"oth\">508.31<\/td><td class=\"oth\">355.10<\/td><\/tr>\n<tr><td class=\"num\">58.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tylvalosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63409-12-1\/Tylvalosin\" target=\"_blank\" rel=\"noopener\">Tylvalosin<\/a><\/td><td class=\"oth\">6.19<\/td><td class=\"oth\">50<\/td><td class=\"oth\">1042.71<\/td><td class=\"oth\">814.46<\/td><td class=\"oth\">640.39<\/td><\/tr>\n<tr><td class=\"num\">59.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cloxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-72-3\/Cloxacillin\" target=\"_blank\" rel=\"noopener\">Cloxacillin<\/a><\/td><td class=\"oth\">6.39<\/td><td class=\"oth\">100<\/td><td class=\"oth\">436.15<\/td><td class=\"oth\">277.06<\/td><td class=\"oth\">160.05<\/td><\/tr>\n<tr><td class=\"num\">60.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nafcillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/147-52-4\/Nafcillin\" target=\"_blank\" rel=\"noopener\">Nafcillin<\/a><\/td><td class=\"oth\">6.60<\/td><td class=\"oth\">25<\/td><td class=\"oth\">415.19<\/td><td class=\"oth\">199.09<\/td><td class=\"oth\">171.06<\/td><\/tr>\n<tr><td class=\"num\">61.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Dicloxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3116-76-5\/Dicloxacillin\" target=\"_blank\" rel=\"noopener\">Dicloxacillin<\/a><\/td><td class=\"oth\">6.96<\/td><td class=\"oth\">100<\/td><td class=\"oth\">470.11<\/td><td class=\"oth\">160.05<\/td><td class=\"oth\">311.02<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">*Acquired in negative ion mode<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0506\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0506\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5\u2013300 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>4.50<\/td><td>0.4<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>7.00<\/td><td>0.4<\/td><td>45<\/td><td>55<\/td><\/tr><tr><td>7.01<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+\/ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>Scheduled MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>1. Positive and negative polarity data were collected simultaneously from a single injection.<br \/>2. Amphenicol compounds (chloramphenicol, thiamphenicol, and florfenicol) were detected with negative polarity.<br \/>3. The MRM was scheduled at +\/- 20 to 30 seconds for each analyte. <br \/>4. Multiclass antibiotics include penicillin, cephalosporin, tetracycline, sulfonamide, macrolide, lincosamide, streptogramin, amphenicol, and quinolone.<br \/>**The retention time for Tylosin is noted in the peak list; however, it was not included in the chromatogram.<br \/><br \/>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0506.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 1:<\/strong>\u00a0Macrolidi, lincosamidi e streptogramine in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0502.png\" alt=\"Macrolide, Lincosamide, and Streptogramin Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0502<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tildipirosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/328898-40-4\/Tildipirosin\" target=\"_blank\" rel=\"noopener\">Tildipirosin<\/a><\/td><td class=\"oth\">1.01<\/td><td class=\"oth\">200<\/td><td class=\"oth\">734.59<\/td><td class=\"oth\">561.45<\/td><td class=\"oth\">204.15<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lincomycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/154-21-2\/Lincomycin\" target=\"_blank\" rel=\"noopener\">Lincomycin<\/a><\/td><td class=\"oth\">1.11<\/td><td class=\"oth\">50<\/td><td class=\"oth\">407.32<\/td><td class=\"oth\">359.23<\/td><td class=\"oth\">389.28<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tulathromycin A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/217500-96-4\/Tulathromycin A\" target=\"_blank\" rel=\"noopener\">Tulathromycin A<\/a><\/td><td class=\"oth\">1.19<\/td><td class=\"oth\">100<\/td><td class=\"oth\">806.65<\/td><td class=\"oth\">577.42<\/td><td class=\"oth\">420.31<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Spiramycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/8025-81-8\/Spiramycin\" target=\"_blank\" rel=\"noopener\">Spiramycin<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">200<\/td><td class=\"oth\">843.64<\/td><td class=\"oth\">540.36<\/td><td class=\"oth\">699.48<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Pirlymycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79548-73-5\/Pirlymycin\" target=\"_blank\" rel=\"noopener\">Pirlymycin<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">20<\/td><td class=\"oth\">411.32<\/td><td class=\"oth\">363.18<\/td><td class=\"oth\">327.21<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Gamithromycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/145435-72-9\/Gamithromycin\" target=\"_blank\" rel=\"noopener\">Gamithromycin<\/a><\/td><td class=\"oth\">1.50<\/td><td class=\"oth\">100<\/td><td class=\"oth\">777.63<\/td><td class=\"oth\">619.52<\/td><td class=\"oth\">601.45<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tilmicosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108050-54-0\/Tilmicosin\" target=\"_blank\" rel=\"noopener\">Tilmicosin<\/a><\/td><td class=\"oth\">1.58<\/td><td class=\"oth\">100<\/td><td class=\"oth\">869.72<\/td><td class=\"oth\">696.50<\/td><td class=\"oth\">522.42<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Erythromycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/114-07-8\/Erythromycin\" target=\"_blank\" rel=\"noopener\">Erythromycin<\/a><\/td><td class=\"oth\">1.80<\/td><td class=\"oth\">25<\/td><td class=\"oth\">734.64<\/td><td class=\"oth\">576.40<\/td><td class=\"oth\">558.38<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tylosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1401-69-0\/Tylosin\" target=\"_blank\" rel=\"noopener\">Tylosin<\/a><\/td><td class=\"oth\">1.87<\/td><td class=\"oth\">100<\/td><td class=\"oth\">916.62<\/td><td class=\"oth\">772.49<\/td><td class=\"oth\">598.36<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tylvalosin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63409-12-1\/Tylvalosin\" target=\"_blank\" rel=\"noopener\">Tylvalosin<\/a><\/td><td class=\"oth\">2.30<\/td><td class=\"oth\">50<\/td><td class=\"oth\">1042.71<\/td><td class=\"oth\">814.46<\/td><td class=\"oth\">640.39<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Virginiamycin M1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21411-53-0\/Virginiamycin M1\" target=\"_blank\" rel=\"noopener\">Virginiamycin M1<\/a><\/td><td class=\"oth\">2.45<\/td><td class=\"oth\">50<\/td><td class=\"oth\">526.43<\/td><td class=\"oth\">508.31<\/td><td class=\"oth\">355.10<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0502\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0502\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>20\u2013200 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>3.00<\/td><td>0.4<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>3.01<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0502.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 2:<\/strong>\u00a0Amfenicoli e tetracicline in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0504.png\" alt=\"Amphenicol and Tetracycline Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0504<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxytetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-57-2\/Oxytetracycline\" target=\"_blank\" rel=\"noopener\">Oxytetracycline<\/a><\/td><td class=\"oth\">1.28<\/td><td class=\"oth\">25<\/td><td class=\"oth\">461.27<\/td><td class=\"oth\">426.15<\/td><td class=\"oth\">443.32<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-54-8\/Tetracycline\" target=\"_blank\" rel=\"noopener\">Tetracycline<\/a><\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">25<\/td><td class=\"oth\">445.28<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">427.32<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Thiamphenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/90-91-5\/Thiamphenicol\" target=\"_blank\" rel=\"noopener\">Thiamphenicol*<\/a><\/td><td class=\"oth\">1.48<\/td><td class=\"oth\">200<\/td><td class=\"oth\">354.16<\/td><td class=\"oth\">290.04<\/td><td class=\"oth\">184.98<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlortetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-62-5\/Chlortetracycline\" target=\"_blank\" rel=\"noopener\">Chlortetracycline<\/a><\/td><td class=\"oth\">1.56<\/td><td class=\"oth\">25<\/td><td class=\"oth\">479.27<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">371.06<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Florfenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73231-34-2\/Florfenicol\" target=\"_blank\" rel=\"noopener\">Florfenicol*<\/a><\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">200<\/td><td class=\"oth\">356.10<\/td><td class=\"oth\">336.02<\/td><td class=\"oth\">184.98<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chloramphenicol*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-75-7\/Chloramphenicol\" target=\"_blank\" rel=\"noopener\">Chloramphenicol*<\/a><\/td><td class=\"oth\">1.95<\/td><td class=\"oth\">200<\/td><td class=\"oth\">321.16<\/td><td class=\"oth\">151.99<\/td><td class=\"oth\">257.04<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">*Acquired in negative ion mode.<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0504\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0504\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25\u2013200 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>3.00<\/td><td>0.4<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>3.01<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+\/ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Tetracyclines and amphenicols were analyzed with ESI+ and ESI- mode, respectively.<br \/><br \/>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0504.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 3:<\/strong>\u00a0Chinoloni in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0505.png\" alt=\"Quinolone Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0505<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Marbofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/115550-35-1\/Marbofloxacin\" target=\"_blank\" rel=\"noopener\">Marbofloxacin<\/a><\/td><td class=\"oth\">1.28<\/td><td class=\"oth\">10<\/td><td class=\"oth\">363.20<\/td><td class=\"oth\">72.11<\/td><td class=\"oth\">320.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norfloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70458-96-7\/Norfloxacin\" target=\"_blank\" rel=\"noopener\">Norfloxacin<\/a><\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">20<\/td><td class=\"oth\">320.23<\/td><td class=\"oth\">276.20<\/td><td class=\"oth\">233.13<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/82419-36-1\/Ofloxacin\" target=\"_blank\" rel=\"noopener\">Ofloxacin<\/a><\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">10<\/td><td class=\"oth\">362.21<\/td><td class=\"oth\">318.20<\/td><td class=\"oth\">261.15<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ciprofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/85721-33-1\/Ciprofloxacin\" target=\"_blank\" rel=\"noopener\">Ciprofloxacin<\/a><\/td><td class=\"oth\">1.35<\/td><td class=\"oth\">20<\/td><td class=\"oth\">332.18<\/td><td class=\"oth\">288.22<\/td><td class=\"oth\">245.15<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Danofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/112398-08-0\/Danofloxacin\" target=\"_blank\" rel=\"noopener\">Danofloxacin<\/a><\/td><td class=\"oth\">1.40<\/td><td class=\"oth\">20<\/td><td class=\"oth\">358.22<\/td><td class=\"oth\">340.16<\/td><td class=\"oth\">314.21<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Enrofloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/93106-60-6\/Enrofloxacin\" target=\"_blank\" rel=\"noopener\">Enrofloxacin<\/a><\/td><td class=\"oth\">1.44<\/td><td class=\"oth\">10<\/td><td class=\"oth\">360.29<\/td><td class=\"oth\">316.22<\/td><td class=\"oth\">245.13<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Orbifloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/113617-63-3\/Orbifloxacin\" target=\"_blank\" rel=\"noopener\">Orbifloxacin<\/a><\/td><td class=\"oth\">1.47<\/td><td class=\"oth\">10<\/td><td class=\"oth\">396.22<\/td><td class=\"oth\">352.17<\/td><td class=\"oth\">226.12<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sarafloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98105-99-8\/Sarafloxacin\" target=\"_blank\" rel=\"noopener\">Sarafloxacin<\/a><\/td><td class=\"oth\">1.55<\/td><td class=\"oth\">10<\/td><td class=\"oth\">386.20<\/td><td class=\"oth\">342.20<\/td><td class=\"oth\">368.15<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Difloxacin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98106-17-3\/Difloxacin\" target=\"_blank\" rel=\"noopener\">Difloxacin<\/a><\/td><td class=\"oth\">1.57<\/td><td class=\"oth\">10<\/td><td class=\"oth\">400.23<\/td><td class=\"oth\">356.17<\/td><td class=\"oth\">299.13<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxolinic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14698-29-4\/Oxolinic acid\" target=\"_blank\" rel=\"noopener\">Oxolinic acid<\/a><\/td><td class=\"oth\">2.23<\/td><td class=\"oth\">5<\/td><td class=\"oth\">262.10<\/td><td class=\"oth\">244.06<\/td><td class=\"oth\">215.96<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flumequine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/42835-25-6\/Flumequine\" target=\"_blank\" rel=\"noopener\">Flumequine<\/a><\/td><td class=\"oth\">2.78<\/td><td class=\"oth\">5<\/td><td class=\"oth\">262.15<\/td><td class=\"oth\">244.11<\/td><td class=\"oth\">202.03<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0505\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0505\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5\u201320 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>3.00<\/td><td>0.4<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>3.01<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0505.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 4:<\/strong>\u00a0Penicilline, cefalosporine, e tetracicline in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0500.png\" alt=\"Penicillin, Cephalosporin, and Tetracycline Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0500<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desacetyl cephapirin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/38115-21-8\/Desacetyl cephapirin\" target=\"_blank\" rel=\"noopener\">Desacetyl cephapirin<\/a><\/td><td class=\"oth\">0.68<\/td><td class=\"oth\">150<\/td><td class=\"oth\">382.03<\/td><td class=\"oth\">111.92<\/td><td class=\"oth\">124.21<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amoxicillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26787-78-0\/Amoxicillin\" target=\"_blank\" rel=\"noopener\">Amoxicillin<\/a><\/td><td class=\"oth\">0.89<\/td><td class=\"oth\">100<\/td><td class=\"oth\">366.24<\/td><td class=\"oth\">349.10<\/td><td class=\"oth\">208.07<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cephapirin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21593-23-7\/Cephapirin\" target=\"_blank\" rel=\"noopener\">Cephapirin<\/a><\/td><td class=\"oth\">1.05<\/td><td class=\"oth\">50<\/td><td class=\"oth\">424.17<\/td><td class=\"oth\">292.08<\/td><td class=\"oth\">124.14<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desfuroyl ceftiofur cysteine disulfide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/158039-15-7\/Desfuroyl ceftiofur cysteine disulfide\" target=\"_blank\" rel=\"noopener\">Desfuroyl ceftiofur cysteine disulfide<\/a><\/td><td class=\"oth\">1.06<\/td><td class=\"oth\">300<\/td><td class=\"oth\">549.16<\/td><td class=\"oth\">183.02<\/td><td class=\"oth\">126.00<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefquinome\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/84957-30-2\/Cefquinome\" target=\"_blank\" rel=\"noopener\">Cefquinome<\/a><\/td><td class=\"oth\">1.16<\/td><td class=\"oth\">200<\/td><td class=\"oth\">529.19<\/td><td class=\"oth\">134.10<\/td><td class=\"oth\">125.12<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ampicillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/69-52-3\/Ampicillin\" target=\"_blank\" rel=\"noopener\">Ampicillin<\/a><\/td><td class=\"oth\">1.18<\/td><td class=\"oth\">50<\/td><td class=\"oth\">350.19<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">160.06<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefalexin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23325-78-2\/Cefalexin\" target=\"_blank\" rel=\"noopener\">Cefalexin<\/a><\/td><td class=\"oth\">1.19<\/td><td class=\"oth\">100<\/td><td class=\"oth\">348.10<\/td><td class=\"oth\">158.05<\/td><td class=\"oth\">174.05<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxytetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-57-2\/Oxytetracycline\" target=\"_blank\" rel=\"noopener\">Oxytetracycline<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">50<\/td><td class=\"oth\">461.27<\/td><td class=\"oth\">426.15<\/td><td class=\"oth\">443.32<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefalonium\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5575-21-3\/Cefalonium\" target=\"_blank\" rel=\"noopener\">Cefalonium<\/a><\/td><td class=\"oth\">1.29<\/td><td class=\"oth\">100<\/td><td class=\"oth\">459.16<\/td><td class=\"oth\">337.03<\/td><td class=\"oth\">123.10<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-54-8\/Tetracycline\" target=\"_blank\" rel=\"noopener\">Tetracycline<\/a><\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">50<\/td><td class=\"oth\">445.28<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">427.32<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefacetrile\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10206-21-0\/Cefacetrile\" target=\"_blank\" rel=\"noopener\">Cefacetrile<\/a><\/td><td class=\"oth\">1.37<\/td><td class=\"oth\">300<\/td><td class=\"oth\">362.07<\/td><td class=\"oth\">258.08<\/td><td class=\"oth\">178.01<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\">Cefazolin<\/td><td class=\"oth\">1.50<\/td><td class=\"oth\">100<\/td><td class=\"oth\">455.10<\/td><td class=\"oth\">323.06<\/td><td class=\"oth\">295.09<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlortetracycline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-62-5\/Chlortetracycline\" target=\"_blank\" rel=\"noopener\">Chlortetracycline<\/a><\/td><td class=\"oth\">1.54<\/td><td class=\"oth\">50<\/td><td class=\"oth\">479.27<\/td><td class=\"oth\">154.07<\/td><td class=\"oth\">371.06<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cefoperazone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/62893-19-0\/Cefoperazone\" target=\"_blank\" rel=\"noopener\">Cefoperazone<\/a><\/td><td class=\"oth\">1.69<\/td><td class=\"oth\">100<\/td><td class=\"oth\">646.26<\/td><td class=\"oth\">143.07<\/td><td class=\"oth\">148.02<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ceftiofur\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80370-57-6\/Ceftiofur\" target=\"_blank\" rel=\"noopener\">Ceftiofur<\/a><\/td><td class=\"oth\">1.85<\/td><td class=\"oth\">50<\/td><td class=\"oth\">524.14<\/td><td class=\"oth\">241.08<\/td><td class=\"oth\">125.24<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Penicillin G\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-33-6\/Penicillin G\" target=\"_blank\" rel=\"noopener\">Penicillin G<\/a><\/td><td class=\"oth\">2.18<\/td><td class=\"oth\">100<\/td><td class=\"oth\">335.18<\/td><td class=\"oth\">176.07<\/td><td class=\"oth\">160.07<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Penicillin V\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/87-08-1\/Penicillin V\" target=\"_blank\" rel=\"noopener\">Penicillin V<\/a><\/td><td class=\"oth\">2.33<\/td><td class=\"oth\">100<\/td><td class=\"oth\">351.10<\/td><td class=\"oth\">160.06<\/td><td class=\"oth\">114.07<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66-79-5\/Oxacillin\" target=\"_blank\" rel=\"noopener\">Oxacillin<\/a><\/td><td class=\"oth\">2.44<\/td><td class=\"oth\">100<\/td><td class=\"oth\">402.15<\/td><td class=\"oth\">160.05<\/td><td class=\"oth\">114.06<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cloxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-72-3\/Cloxacillin\" target=\"_blank\" rel=\"noopener\">Cloxacillin<\/a><\/td><td class=\"oth\">2.56<\/td><td class=\"oth\">100<\/td><td class=\"oth\">436.15<\/td><td class=\"oth\">277.06<\/td><td class=\"oth\">160.05<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nafcillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/147-52-4\/Nafcillin\" target=\"_blank\" rel=\"noopener\">Nafcillin<\/a><\/td><td class=\"oth\">2.63<\/td><td class=\"oth\">25<\/td><td class=\"oth\">415.19<\/td><td class=\"oth\">199.09<\/td><td class=\"oth\">171.06<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Dicloxacillin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3116-76-5\/Dicloxacillin\" target=\"_blank\" rel=\"noopener\">Dicloxacillin<\/a><\/td><td class=\"oth\">2.76<\/td><td class=\"oth\">100<\/td><td class=\"oth\">470.11<\/td><td class=\"oth\">160.05<\/td><td class=\"oth\">311.02<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0500\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0500\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25\u2013300 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>3.00<\/td><td>0.4<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>3.01<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0500.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 5:<\/strong>\u00a0Sulfamidici in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0501.png\" alt=\"Sulfonamide Antibiotics on Raptor C18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0501<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfanilamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-74-1\/Sulfanilamide\" target=\"_blank\" rel=\"noopener\">Sulfanilamide<\/a><\/td><td class=\"oth\">0.83<\/td><td class=\"oth\">200<\/td><td class=\"oth\">172.98<\/td><td class=\"oth\">93.07<\/td><td class=\"oth\">75.23<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadiazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68-35-9\/Sulfadiazine\" target=\"_blank\" rel=\"noopener\">Sulfadiazine<\/a><\/td><td class=\"oth\">1.45<\/td><td class=\"oth\">20<\/td><td class=\"oth\">251.18<\/td><td class=\"oth\">156.04<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfathiazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-14-0\/Sulfathiazole\" target=\"_blank\" rel=\"noopener\">Sulfathiazole<\/a><\/td><td class=\"oth\">1.60<\/td><td class=\"oth\">10<\/td><td class=\"oth\">256.16<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfapyridine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-83-2\/Sulfapyridine\" target=\"_blank\" rel=\"noopener\">Sulfapyridine<\/a><\/td><td class=\"oth\">1.67<\/td><td class=\"oth\">10<\/td><td class=\"oth\">250.13<\/td><td class=\"oth\">156.10<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamerazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-79-7\/Sulfamerazine\" target=\"_blank\" rel=\"noopener\">Sulfamerazine<\/a><\/td><td class=\"oth\">1.79<\/td><td class=\"oth\">20<\/td><td class=\"oth\">265.08<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">92.08<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-68-1\/Sulfamethazine\" target=\"_blank\" rel=\"noopener\">Sulfamethazine<\/a><\/td><td class=\"oth\">2.07<\/td><td class=\"oth\">10<\/td><td class=\"oth\">279.23<\/td><td class=\"oth\">186.08<\/td><td class=\"oth\">124.08<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethizole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-82-1\/Sulfamethizole\" target=\"_blank\" rel=\"noopener\">Sulfamethizole<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">10<\/td><td class=\"oth\">271.17<\/td><td class=\"oth\">156.02<\/td><td class=\"oth\">108.02<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethoxypyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-35-3\/Sulfamethoxypyridazine\" target=\"_blank\" rel=\"noopener\">Sulfamethoxypyridazine<\/a><\/td><td class=\"oth\">2.16<\/td><td class=\"oth\">10<\/td><td class=\"oth\">281.14<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">126.07<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfachlorpyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-32-0\/Sulfachlorpyridazine\" target=\"_blank\" rel=\"noopener\">Sulfachlorpyridazine<\/a><\/td><td class=\"oth\">2.55<\/td><td class=\"oth\">20<\/td><td class=\"oth\">285.05<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2447-57-6\/Sulfadoxine\" target=\"_blank\" rel=\"noopener\">Sulfadoxine<\/a><\/td><td class=\"oth\">2.75<\/td><td class=\"oth\">10<\/td><td class=\"oth\">311.17<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/723-46-6\/Sulfamethoxazole\" target=\"_blank\" rel=\"noopener\">Sulfamethoxazole<\/a><\/td><td class=\"oth\">2.78<\/td><td class=\"oth\">20<\/td><td class=\"oth\">254.18<\/td><td class=\"oth\">155.98<\/td><td class=\"oth\">147.06<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaethoxypyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/963-14-4\/Sulfaethoxypyridazine\" target=\"_blank\" rel=\"noopener\">Sulfaethoxypyridazine<\/a><\/td><td class=\"oth\">2.84<\/td><td class=\"oth\">20<\/td><td class=\"oth\">295.17<\/td><td class=\"oth\">267.07<\/td><td class=\"oth\">156.03<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfisoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-69-5\/Sulfisoxazole\" target=\"_blank\" rel=\"noopener\">Sulfisoxazole<\/a><\/td><td class=\"oth\">2.98<\/td><td class=\"oth\">20<\/td><td class=\"oth\">268.14<\/td><td class=\"oth\">156.03<\/td><td class=\"oth\">113.10<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadimethoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/122-11-2\/Sulfadimethoxine\" target=\"_blank\" rel=\"noopener\">Sulfadimethoxine<\/a><\/td><td class=\"oth\">3.37<\/td><td class=\"oth\">10<\/td><td class=\"oth\">311.17<\/td><td class=\"oth\">156.09<\/td><td class=\"oth\">108.09<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaquinoxaline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-40-5\/Sulfaquinoxaline\" target=\"_blank\" rel=\"noopener\">Sulfaquinoxaline<\/a><\/td><td class=\"oth\">3.40<\/td><td class=\"oth\">20<\/td><td class=\"oth\">301.18<\/td><td class=\"oth\">156.04<\/td><td class=\"oth\">108.02<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0501\" rel=\"noopener\">cat.# 9304A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9304A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0501\" rel=\"noopener\">cat.# 9304A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>10\u2013200 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>3.50<\/td><td>0.4<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>3.51<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0501.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 6:<\/strong>\u00a0Ionofori in LC-MS\/MS sulla Raptor C18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0503.png\" alt=\"Ionophore Antibiotics on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0503<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lasalocid A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25999-31-9\/Lasalocid A\" target=\"_blank\" rel=\"noopener\">Lasalocid A<\/a><\/td><td class=\"oth\">1.92<\/td><td class=\"oth\">100<\/td><td class=\"oth\">613.42<\/td><td class=\"oth\">377.28<\/td><td class=\"oth\">595.40<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Monensin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17090-79-8\/Monensin\" target=\"_blank\" rel=\"noopener\">Monensin<\/a><\/td><td class=\"oth\">2.12<\/td><td class=\"oth\">100<\/td><td class=\"oth\">693.50<\/td><td class=\"oth\">675.44<\/td><td class=\"oth\">461.30<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Salinomycin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/53003-10-4\/Salinomycin\" target=\"_blank\" rel=\"noopener\">Salinomycin<\/a><\/td><td class=\"oth\">2.19<\/td><td class=\"oth\">100<\/td><td class=\"oth\">773.57<\/td><td class=\"oth\">431.24<\/td><td class=\"oth\">531.39<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Maduramicin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79356-08-4\/Maduramicin\" target=\"_blank\" rel=\"noopener\">Maduramicin<\/a><\/td><td class=\"oth\">2.30<\/td><td class=\"oth\">100<\/td><td class=\"oth\">939.65<\/td><td class=\"oth\">877.58<\/td><td class=\"oth\">473.31<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Narasin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55134-13-9\/Narasin\" target=\"_blank\" rel=\"noopener\">Narasin<\/a><\/td><td class=\"oth\">2.58<\/td><td class=\"oth\">100<\/td><td class=\"oth\">787.59<\/td><td class=\"oth\">431.27<\/td><td class=\"oth\">531.35<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0503\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0503\" rel=\"noopener\">cat.# 9309A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:methanol (10:90)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.5% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.5% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>3.00<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>3.01<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0503.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>L&#8217;uso di antibiotici sugli animali destinati alla produzione alimentare \u00e8 un problema di salute e sicurezza pubblica in quanto potrebbero svilupparsi batteri farmacoresistenti. Poich\u00e9 le agenzie di sicurezza alimentare di tutto il mondo attuano politiche sull\u2019uso di questi farmaci, \u00e8 necessario disporre di un metodo analitico sensibile, efficiente e affidabile per le diverse classi di antibiotici; la colonna LC Raptor C18 di Restek soddisfa questa esigenza.<\/p>\n","protected":false},"author":11,"featured_media":7061,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[788],"tags":[],"industries-application":[2290],"post-badge":[],"resource-type":[],"product-library":[2535,2517],"resource-technique":[2334,2334,2364],"ppma_author":[414],"class_list":{"0":"post-43623","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","6":"hentry","7":"category-articoli","8":"industries-application-food-beverage-it","9":"product-library-colonne-lc","10":"product-library-prodotti-per-cromatografia-liquida","11":"resource-technique-cromatografia-liquida-lc","13":"resource-technique-ms-ms-it"},"acf":[],"taxonomy_info":{"category":[{"value":788,"label":"Articoli"}],"industries-application":[{"value":2290,"label":"Food &amp; 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