{"id":43836,"date":"2021-11-11T00:00:00","date_gmt":"2021-11-11T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/raptor-polar-x-separa-una-vasta-gamma-di-analiti-polari-con-uninnovativa-fase-stazionaria-ibrida\/"},"modified":"2026-01-28T22:11:00","modified_gmt":"2026-01-28T22:11:00","slug":"raptor-polar-x-separa-una-vasta-gamma-di-analiti-polari-con-uninnovativa-fase-stazionaria-ibrida","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/articoli\/gnss3195-it\/raptor-polar-x-separa-una-vasta-gamma-di-analiti-polari-con-uninnovativa-fase-stazionaria-ibrida","title":{"rendered":"Raptor Polar X: Separa una vasta gamma di analiti polari con un\u2019innovativa fase stazionaria ibrida"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Ideale per evitare lunghi processi di derivatizzazione o complessi accoppiamenti ionici.<\/li>\n\n\n\n<li>Possibilit\u00e0 di passare da una modalit\u00e0 di ritenzione a un\u2019altra con semplici modifiche della fase mobile e con tempi di equilibratura brevi.<\/li>\n\n\n\n<li>Ideale per aumentare la sensibilit\u00e0 e la selettivit\u00e0 delle analisi LC-MS.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Semplifica l\u2019analisi dei composti polari<\/h2>\n\n\n\n<p>Analizzare i composti polari utilizzando la cromatografia liquida \u00e8 da sempre una procedura impegnativa. Scarsa ritenzione e forma del picco, fasi mobili complesse che potrebbero essere incompatibili con MS, lunghi tempi di equilibratura, scarsa sensibilit\u00e0 e necessit\u00e0 di derivatizzare il campione sono tutte criticit\u00e0 che riducono la produttivit\u00e0 e l\u2019efficienza del laboratorio. Lo sviluppo di un\u2019innovativa colonna studiata appositamente per l\u2019analisi di una vasta gamma di composti polari consente agli scienziati di evitare queste problematiche sfruttando appieno le potenzialit\u00e0 della cromatografia.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">La vera forza della cromatografia<\/h2>\n\n\n\n<p>Uno degli aspetti pi\u00f9 significativi, ma meno compresi, che influenzano le prestazioni del metodo \u00e8 l\u2019importanza di utilizzare la fase stazionaria adatta a una particolare separazione. Per l\u2019analisi dei composti polari, le colonne a fase inversa non offrono un\u2019adeguata ritenzione se non con il ricorso a fasi mobili complesse o alla derivatizzazione del campione per compensare la mancanza di interazioni efficaci tra gli analiti e la colonna. Tuttavia, abbinare gli analiti a una fase stazionaria con un potere risolutivo ad hoc permetter\u00e0 di evitare complesse procedure di preparazione del campione, risparmiare tempo e denaro e ridurre il rischio di errore.<\/p>\n\n\n\n<p>La fase stazionaria delle colonne Raptor Polar X \u00e8 studiata appositamente per la ritenzione selettiva degli analiti polari attraverso il bilanciamento di due meccanismi di ritenzione. Si tratta di una fase ibrida e unica, che \u00e8 la soluzione ideale per analizzare una vasta gamma di composti polari, soprattutto quando accoppiata alla spettrometria di massa. Semplifica l\u2019analisi dei composti polari grazie alla selettivit\u00e0 unica garantita dalle colonne Raptor Polar X di Restek.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Un potenziale che va oltre i singoli vantaggi<\/h2>\n\n\n\n<p>Consapevole che i meccanismi di ritenzione pi\u00f9 usati per i composti polari sono la cromatografia a interazione idrofilica (HILIC) e lo scambio ionico, Restek ha sviluppato un\u2019innovativa fase stazionaria che combina entrambe le modalit\u00e0 in un unico ligando. Poich\u00e9 quest\u2019unico ligando si lega alle particelle superficialmente porose, le colonne Raptor Polar X sono in grado di effettuare una ritenzione affidabile e una separazione efficiente di una vasta gamma di analiti polari.<\/p>\n\n\n\n<p>Le colonne tradizionali ad applicazione specifica favoriscono in genere un solo metodo di ritenzione, e i vantaggi nella ritenzione di un tipo di composto polare sono conquistati a discapito della performance relativa agli altri. Ora&nbsp;invece, grazie all\u2019innovativa fase della colonna Raptor Polar X (in attesa di brevetto) sono disponibili due meccanismi di ritenzione indipendenti per una ritenzione flessibile e perfettamente bilanciata (Figura 1). Semplici variazioni nelle condizioni della fase mobile permettono agli analisti di passare da una modalit\u00e0 all\u2019altra e regolare in modo selettivo la ritenzione per i composti d\u2019interesse senza dover attendere i lunghi tempi di equilibratura prima dell\u2019utilizzo o tra un campione e l\u2019altro.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 1:<\/strong>\u00a0La colonna Raptor Polar X passa velocemente e facilmente da una modalit\u00e0 di ritenzione polare all\u2019altra grazie a semplici variazioni delle condizioni della fase mobile, offrendo cos\u00ec capacit\u00e0 senza precedenti di ritenzione e separazione di una vasta gamma di composti polari, addirittura nella stessa analisi.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43836_b08307-e1 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43836_b08307-e1\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"620\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-01-768x620.jpg\" alt=\"\" class=\"kb-img wp-image-23302\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-768x620.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-300x242.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1024x826.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1536x1239.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Funzionamento: da una modalit\u00e0 di ritenzione all\u2019altra<\/h2>\n\n\n\n<p>When using a mobile phase with a relatively high-percentage of acetonitrile to water, a layer of water is formed at the surface of the silica into which polar compounds can partition. Partitioning into the water layer results in effective interactions between polar analytes and the ligand bound to the silica surface (Figure 2). The novel Raptor Polar X ligand chemistry makes this technique even easier, allowing for faster-than-ever column equilibration and re-equilibration. This means new columns are ready for use quickly, and sample throughput increases because less re-equilibration time is required between samples.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 2:<\/strong>\u00a0La rapida formazione di uno strato acquoso sulla superficie della silice permette la partizione di un\u2019ampia gamma di composti polari tra l\u2019acetonitrile nella fase mobile e lo strato acquoso. Questa partizione, unitamente alle interazioni con la fase stazionaria, definisce il meccanismo di ritenzione HILIC.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43836_164bc2-bc .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43836_164bc2-bc\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"411\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-02-768x411.jpg\" alt=\"\" class=\"kb-img wp-image-23308\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-768x411.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-300x161.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-1024x548.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-1536x823.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p>For smaller polar compounds, these high-percentage acetonitrile conditions generate strong interactions between the analytes and the stationary phase, leading to excellent retention of small, highly polar, charged compounds. To fine-tune retention, simply increase the amount of water in the mobile phase. This will draw the charged, polar compounds into the mobile phase, effectively eluting them. When moving to a higher percent water mobile phase, the distinct HILIC partitioning retention will diminish and the stationary phase\u2019s ion-exchange characteristics will become the dominant retention mechanism (Figure 3).<\/p>\n\n\n\n<p>Per i composti polari di dimensioni inferiori, questa elevata percentuale di acetonitrile genera forti interazioni tra gli analiti e la fase stazionaria, dando luogo a un\u2019ottima ritenzione dei composti piccoli, molto polari e caricati. Per&nbsp;regolare la ritenzione basta semplicemente aumentare la quantit\u00e0 di acqua nella fase mobile. Questo trasciner\u00e0 i&nbsp;composti polari caricati nella fase mobile, consentendone un\u2019efficace eluizione. Passando a una fase mobile con&nbsp;una percentuale pi\u00f9 elevata di acqua, la ritenzione dovuta alla partizione HILIC diminuir\u00e0 e le caratteristiche di&nbsp;scambio ionico della fase stazionaria si riveleranno il meccanismo di ritenzione prevalente (Figura 3).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 3:<\/strong>\u00a0In condizioni di fase mobile con un maggiore contenuto di acqua, i meccanismi di scambio ionico subentrano come metodo di ritenzione dominante per l\u2019analisi dei composti polari.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43836_1dbdef-0c .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43836_1dbdef-0c\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"367\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-03-768x367.jpg\" alt=\"\" class=\"kb-img wp-image-23326\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-768x367.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-300x143.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1024x489.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1536x734.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Cambio della modalit\u00e0 di ritenzione: un esempio<\/h2>\n\n\n\n<p>Per illustrare l\u2019azione di questi principi, la Figura 4 mostra come due tipi diversi di composti polari interagiscono con la fase stazionaria e come le loro risposte sono influenzate da semplici variazioni della fase mobile. In questo esempio, abbiamo analizzato due vitamine idrosolubili: la vitamina B3 (niacina), che \u00e8 un acido organico, e la vitamina B1 (tiamina), che ha una carica positiva permanente.<\/p>\n\n\n\n<p>Nel cromatogramma in alto \u00e8 stata utilizzata una fase mobile altamente organica, che favorisce la separazione HILIC. Utilizzando la colonna Raptor Polar X con queste condizioni, si forma rapidamente uno strato acquoso sulla superficie delle particelle di silice. Questo favorisce la partizione dei composti polari dallo strato di acetonitrile nello strato acquoso, dove questi possono anche interagire con la superficie della silice e la fase stazionaria. In questo modo, i composti polari vengono trattenuti grazie alle interazioni idrofiliche con la fase stazionaria e vengono ancora eluiti in condizioni di fase mobile altamente organica. Ci\u00f2 \u00e8 di grande aiuto per l\u2019analisi LC-MS\/MS grazie alla migliore desolvatazione e a una maggiore efficienza della ionizzazione.<\/p>\n\n\n\n<p>Il cromatogramma in basso evidenzia i&nbsp;cambiamenti cromatografici rilevati quando si cambia il meccanismo di ritenzione passando a una fase mobile con una percentuale di acqua pi\u00f9 elevata. Pi\u00f9&nbsp;ci allontaniamo dalle condizioni ideali per la HILIC, meno vengono trattenute sia&nbsp;la vitamina B1 che la vitamina B3. Questo \u00e8 dovuto al fatto che, con l\u2019aumento della percentuale di acqua nella fase mobile, le&nbsp;condizioni si allontanano dalla ritenzione HILIC per avvicinarsi alle caratteristiche di ritenzione a scambio ionico. Anche l\u2019ordine di eluizione cambia perch\u00e9 in condizioni di scambio ionico la vitamina B1 \u00e8 meno trattenuta della vitamina B3.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 4:<\/strong>\u00a0Semplici variazioni alle fasi mobili, facili da preparare e compatibili con MS, possono enfatizzare o de-enfatizzare i vari meccanismi di ritenzione disponibili con la nuova fase stazionaria della colonna Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.png\" alt=\"Raptor Polar X Retention Mode Comparison: Buffered B Vitamins with 95% vs. 60% Mobile Phase B\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0652<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B3\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-67-6\/Vitamin B3\" target=\"_blank\" rel=\"noopener\">Vitamin B3<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-43-8\/Vitamin B1\" target=\"_blank\" rel=\"noopener\">Vitamin B1<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0652\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in acetonitrile<\/td><\/tr><td><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.5 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Mobile phase A: Water, 5 mM ammonium formate, 0.1% formic acid<br \/>Mobile phase B: Acetonitrile, 0.1% formic acid<br \/>Top chromatogram: 95% B, 10 min run, 2 \u03bcL injection (100 ppm B3, 0.01 ppm B1)<br \/>Bottom chromatogram: 60% B, 5 min run, 0.5 \u03bcL injection (100 ppm B3, 0.1 ppm B1)<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Una ritenzione ibrida ben bilanciata permette di analizzare pi\u00f9 composti utilizzando un unico metodo<\/h2>\n\n\n\n<p>In genere le colonne hanno un uso specifico, concentrandosi su un tipo di interazione ben determinato ed escludendo le altre modalit\u00e0 di interazione. Questo approccio funziona bene quando tutti gli analiti target hanno le stesse caratteristiche; tuttavia, i composti polari hanno tratti chimici ampiamente differenti. Nell\u2019analisi dei composti polari, un approccio specifico nella scelta della colonna spesso si traduce nel dover utilizzare pi\u00f9 metodi diversi con colonne o condizioni diverse a seconda dell\u2019analita. Come mostrano gli esempi che seguono, la fase ibrida delle colonne Raptor Polar X \u00e8 un\u2019alternativa migliore perch\u00e9 il profilo di ritenzione multimodale permette di analizzare una vasta gamma di analiti target con lo stesso metodo.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Lista di contaminanti polari ispirata al metodo QuPPe<\/h2>\n\n\n\n<p>Il metodo europeo QuPPe (<strong>Qu<\/strong>ick&nbsp;<strong>P<\/strong>olar&nbsp;<strong>Pe<\/strong>sticides) include una variet\u00e0 di analiti polari, dagli erbicidi polari anionici, come il glifosato e composti correlati, a tutta una serie di oxicloro contaminanti, tra cui il clorato e il perclorato. Come&nbsp;mostra la Figura 5, la colonna Raptor Polar X trattiene e separa rapidamente questo mix eterogeneo, in cui l\u2019ultimo composto eluisce in circa 10,5 minuti e con un tempo totale di corsa di soli 13 minuti. Questa separazione viene eseguita con fasi mobili semplici senza tampone, acidificate con lo 0,5% di acido formico per ridurre l\u2019allargamento e lo scodamento del picco. Il metodo mostrato nella Figura 5 ha permesso anche la separazione dei composti con frammenti di massa simile, come l\u2019AMPA dall\u2019N-acetile AMPA e il fosetil alluminio da acido fosfonico e acido fosforico (un componente della matrice comunemente osservato e fonte di interferenza).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 5:<\/strong>\u00a0in questa analisi dei composti polari, un gruppo diversificato di analiti pu\u00f2 essere separato con successo in una corsa singola grazie alle caratteristiche uniche di ritenzione bilanciata della colonna Raptor Polar X<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.png\" alt=\"17 Polar Pesticide Panel on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0546<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><th style=\"text-align: center;width: 75px\">Precursor 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Aminomethylphosphonic acid (AMPA)<\/td><td class=\"oth\">0.805<\/td><td class=\"oth\">200<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Bialophos<\/td><td class=\"oth\">0.847<\/td><td class=\"oth\">100<\/td><td class=\"oth\">322.2<\/td><td class=\"oth\">88.2<\/td><td class=\"oth\">216.1<\/td><td class=\"oth\">134.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perchlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14797-73-0\/Perchlorate\" target=\"_blank\" rel=\"noopener\">Perchlorate<\/a><\/td><td class=\"oth\">2.593<\/td><td class=\"oth\">5<\/td><td class=\"oth\">101.0<\/td><td class=\"oth\">84.95<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">98.9<\/td><td class=\"oth\">83<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">3.376<\/td><td class=\"oth\">200<\/td><td class=\"oth\">180.2<\/td><td class=\"oth\">85.2<\/td><td class=\"oth\">95.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">3-(Methylphophinico) propionic acid (MPPA)<\/td><td class=\"oth\">4.076<\/td><td class=\"oth\">100<\/td><td class=\"oth\">151.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">107.1<\/td><td class=\"oth\">133.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoracetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoracetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoracetic acid (TFA)<\/a><\/td><td class=\"oth\">4.423<\/td><td class=\"oth\">20<\/td><td class=\"oth\">113.0<\/td><td class=\"oth\">69.1<\/td><td class=\"oth\">19.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">2-Hydroxyethane phosphonic acid (HEPA)<\/td><td class=\"oth\">4.969<\/td><td class=\"oth\">100<\/td><td class=\"oth\">125.1<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">63<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Difluoroacetic acid (DFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/381-73-7\/Difluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Difluoroacetic acid (DFA)<\/a><\/td><td class=\"oth\">5.018<\/td><td class=\"oth\">200<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">51.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14866-68-3\/Chlorate\" target=\"_blank\" rel=\"noopener\">Chlorate<\/a><\/td><td class=\"oth\">5.542<\/td><td class=\"oth\">100<\/td><td class=\"oth\">85.0<\/td><td class=\"oth\">69.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">83.0<\/td><td class=\"oth\">67.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethephon\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/16672-87-0\/Ethephon\" target=\"_blank\" rel=\"noopener\">Ethephon<\/a><\/td><td class=\"oth\">5.564<\/td><td class=\"oth\">200<\/td><td class=\"oth\">143.1<\/td><td class=\"oth\">107.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">6.113<\/td><td class=\"oth\">200<\/td><td class=\"oth\">168.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\">Bromide<\/td><td class=\"oth\">6.423<\/td><td class=\"oth\">2000<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">79.0<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Bromate<\/td><td class=\"oth\">6.771<\/td><td class=\"oth\">600<\/td><td class=\"oth\">129.0<\/td><td class=\"oth\">113<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">127<\/td><td class=\"oth\">111.1<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\">N-acetyl AMPA<\/td><td class=\"oth\">6.932<\/td><td class=\"oth\">200<\/td><td class=\"oth\">152.1<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\">Fosetyl aluminum<\/td><td class=\"oth\">7.775<\/td><td class=\"oth\">80<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">81.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">78.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\">Phosphonic acid<\/td><td class=\"oth\">8.275<\/td><td class=\"oth\">500<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\">N-acetyl glufosinate<\/td><td class=\"oth\">9.980<\/td><td class=\"oth\">200<\/td><td class=\"oth\">222.2<\/td><td class=\"oth\">136.1<\/td><td class=\"oth\">134.1<\/td><td class=\"oth\">59.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0546\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>13<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p>Oltre al metodo sviluppato da Restek illustrato nella Figura 5, anche le colonne analitiche e le precolonne Raptor Polar X sono state sottoposte a valutazione indipendente e incluse nel metodo QuPPe [1]. Vengono impiegate in un metodo per la rilevazione di una vasta gamma di pesticidi polari in LC-MS\/MS in modalit\u00e0 ESI(-).<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Amminoacidi non derivatizzati<\/h2>\n\n\n\n<p>Amino acids are a diverse group of very polar compounds that are commonly analyzed with reversed-phase or ion-exchange chromatography using pre- or post-column derivatization. Direct analysis of underivatized amino acids is challenging due to limited retention and poor chromatographic performance. However, underivatized amino acids with nonpolar, polar, positively charged, and negatively charged side chains are all retained and easily separated in one method on a Raptor Polar X column. Figure 6 shows the analysis of 21 amino acids, including a taurine supplement, in a liquid baby formula matrix using a simple protein precipitation and direct analysis of the resulting extract.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 6:<\/strong>\u00a0I composti con polarit\u00e0 diverse, come questi amminoacidi, possono essere analizzati utilizzando lo stesso metodo su una colonna Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.png\" alt=\"Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0579<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tryptophan\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54-12-6\/Tryptophan\" target=\"_blank\" rel=\"noopener\">Tryptophan<\/a><\/td><td class=\"oth\">1.17<\/td><td class=\"oth\">205.07<\/td><td class=\"oth\">146.08<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenylalanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-91-2\/Phenylalanine\" target=\"_blank\" rel=\"noopener\">Phenylalanine<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">166.13<\/td><td class=\"oth\">120.10<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Leucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-90-5\/Leucine\" target=\"_blank\" rel=\"noopener\">Leucine<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Isoleucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73-32-5\/Isoleucine\" target=\"_blank\" rel=\"noopener\">Isoleucine<\/a><\/td><td class=\"oth\">1.55<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methionine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-51-8\/Methionine\" target=\"_blank\" rel=\"noopener\">Methionine<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">150.07<\/td><td class=\"oth\">104.10<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tyrosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/556-03-6\/Tyrosine\" target=\"_blank\" rel=\"noopener\">Tyrosine<\/a><\/td><td class=\"oth\">1.69<\/td><td class=\"oth\">182.10<\/td><td class=\"oth\">136.08<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Taurine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/107-35-7\/Taurine\" target=\"_blank\" rel=\"noopener\">Taurine<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">126.07<\/td><td class=\"oth\">108.07<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Valine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/516-06-3\/Valine\" target=\"_blank\" rel=\"noopener\">Valine<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">118.13<\/td><td class=\"oth\">72.11<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Proline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/609-36-9\/Proline\" target=\"_blank\" rel=\"noopener\">Proline<\/a><\/td><td class=\"oth\">2.29<\/td><td class=\"oth\">116.13<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-72-7\/Alanine\" target=\"_blank\" rel=\"noopener\">Alanine<\/a><\/td><td class=\"oth\">3.00<\/td><td class=\"oth\">90.03<\/td><td class=\"oth\">44.10<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Threonine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-68-2\/Threonine\" target=\"_blank\" rel=\"noopener\">Threonine<\/a><\/td><td class=\"oth\">3.12<\/td><td class=\"oth\">120.13<\/td><td class=\"oth\">74.08<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glycine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-40-6\/Glycine\" target=\"_blank\" rel=\"noopener\">Glycine<\/a><\/td><td class=\"oth\">3.62<\/td><td class=\"oth\">76.10<\/td><td class=\"oth\">30.11<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-85-9\/Glutamine\" target=\"_blank\" rel=\"noopener\">Glutamine<\/a><\/td><td class=\"oth\">3.87<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.07<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Serine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-84-1\/Serine\" target=\"_blank\" rel=\"noopener\">Serine<\/a><\/td><td class=\"oth\">3.93<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">60.09<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Asparagine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70-47-3\/Asparagine\" target=\"_blank\" rel=\"noopener\">Asparagine<\/a><\/td><td class=\"oth\">4.08<\/td><td class=\"oth\">133.13<\/td><td class=\"oth\">74.07<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Arginine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/74-79-3\/Arginine\" target=\"_blank\" rel=\"noopener\">Arginine<\/a><\/td><td class=\"oth\">4.47<\/td><td class=\"oth\">175.17<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Histidine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-00-1\/Histidine\" target=\"_blank\" rel=\"noopener\">Histidine<\/a><\/td><td class=\"oth\">4.66<\/td><td class=\"oth\">156.07<\/td><td class=\"oth\">110.16<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lysine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-87-1\/Lysine\" target=\"_blank\" rel=\"noopener\">Lysine<\/a><\/td><td class=\"oth\">4.97<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.13<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-65-2\/Glutamic acid\" target=\"_blank\" rel=\"noopener\">Glutamic acid<\/a><\/td><td class=\"oth\">5.89<\/td><td class=\"oth\">148.10<\/td><td class=\"oth\">84.10<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cystine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-89-3\/Cystine\" target=\"_blank\" rel=\"noopener\">Cystine<\/a><\/td><td class=\"oth\">6.10<\/td><td class=\"oth\">241.13<\/td><td class=\"oth\">152.00<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aspartic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-45-8\/Aspartic acid\" target=\"_blank\" rel=\"noopener\">Aspartic acid<\/a><\/td><td class=\"oth\">7.12<\/td><td class=\"oth\">134.07<\/td><td class=\"oth\">74.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0579\" rel=\"noopener\">cat.# 9311A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>20:80 Water:acetonitrile, 0.01 N HCl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>  Endogenous amino acids<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Mobile Phase B: 9:1 Acetonitrile:20 mM ammonium formate in water (pH 3.0) (The ammonium formate concentration is 20 mM relative to the total volume of mobile phase B. See preparation notes for instructions on diluting a 200 mM aqueous starting solution.) <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>3.50<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>8.01<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>10.0<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A 200 \u00b5L aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 \u00b5L of acetonitrile and 10 \u00b5L of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><i>Mobile Phase B Preparation:<\/i> To make 500 mL of mobile phase B, measure ~45 mL of water into a small beaker and add 1 mL of 10 M ammonium formate solution. Adjust pH to 3.0 by adding formic acid and then bring the volume to 50 mL with water. Combine this 50 mL ammonium formate solution (pH 3.0) with 450 mL of acetonitrile to complete the preparation.   <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Ultrashort- to Long-Chain PFAS<\/h2>\n\n\n\n<p>Gli amminoacidi sono un gruppo variegato di composti molto polari comunemente analizzati in cromatografia a fase inversa o&nbsp;a scambio ionico, utilizzando la derivatizzazione pre- o post-colonna. L\u2019analisi diretta degli amminoacidi non derivatizzati \u00e8 complessa a causa della ritenzione limitata e della scarsa prestazione cromatografica. Tuttavia, gli amminoacidi non derivatizzati con catene laterali non polari, polari, con carica positiva e con carica negativa vengono tutti trattenuti e separati facilmente in un unico metodo sulla colonna Raptor Polar X. La Figura\u202f6 mostra l\u2019analisi di 21\u202famminoacidi, incluso un supplemento di taurina, nella matrice liquida di un latte artificiale per bambini utilizzando una semplice precipitazione delle proteine e l\u2019analisi diretta dell\u2019estratto risultante.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 7:<\/strong>\u00a0I PFAS a catena ultra-corta, tradizionali o alternativi analizzati con un unico metodo sulla nuova colonna Raptor Polar X.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.png\" alt=\"Ultrashort- Through Long-Chain and Alternative PFAS on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11CL-PF3OUdS)<\/td><td class=\"oth\">1.25<\/td><td class=\"oth\">400<\/td><td class=\"oth\">630.78<\/td><td class=\"oth\">450.80<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)<\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">400<\/td><td class=\"oth\">530.78<\/td><td class=\"oth\">350.85<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">400<\/td><td class=\"oth\">498.84<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanesulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">400<\/td><td class=\"oth\">398.90<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanesulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanesulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">1.64<\/td><td class=\"oth\">400<\/td><td class=\"oth\">298.97<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoropropanesulfonic acid (PFPrS)<\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">400<\/td><td class=\"oth\">248.97<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">Perfluoroethanesulfonic acid (PFEtS)<\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">400<\/td><td class=\"oth\">198.98<\/td><td class=\"oth\">79.92<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hexafluoropropylene oxide dimer acid (HFPO-DA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13252-13-6\/Hexafluoropropylene oxide dimer acid\" target=\"_blank\" rel=\"noopener\">Hexafluoropropylene oxide dimer acid (HFPO-DA)<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">284.97<\/td><td class=\"oth\">168.92<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">400<\/td><td class=\"oth\">412.90<\/td><td class=\"oth\">368.91<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/919005-14-4\/Ammonium 4,8-dioxa-3H-perfluorononanoate\" target=\"_blank\" rel=\"noopener\">Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)<\/a><\/td><td class=\"oth\">2.15<\/td><td class=\"oth\">400<\/td><td class=\"oth\">376.90<\/td><td class=\"oth\">250.93<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">2.36<\/td><td class=\"oth\">400<\/td><td class=\"oth\">312.97<\/td><td class=\"oth\">268.90<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanoic acid (PFBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-22-4\/Perfluorobutanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanoic acid (PFBA)<\/a><\/td><td class=\"oth\">2.76<\/td><td class=\"oth\">400<\/td><td class=\"oth\">212.97<\/td><td class=\"oth\">168.97<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Perfluoropropionic acid (PFPrA)<\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">163.03<\/td><td class=\"oth\">119.01<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoroacetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoroacetic acid (TFA)<\/a><\/td><td class=\"oth\">3.77<\/td><td class=\"oth\">400<\/td><td class=\"oth\">113.03<\/td><td class=\"oth\">69.01<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0569\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>400  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 10 mM ammonium formate, 0.05% formic acid   <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>60:40 Acetonitrile:methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Passivare o non passivare?<\/h2>\n\n\n\n<p>Rilevare il glifosato in tracce \u00e8 difficile perch\u00e9 questo composto chela con i metalli attivi nel sistema LC. Anche se la colonna Raptor Polar X \u00e8 trattata e arriva in laboratorio pronta per il primo utilizzo, potrebbe essere utile passivare i componenti metallici presenti lungo il percorso del campione nella strumentazione LC. La necessit\u00e0 o meno della passivazione dipende dall\u2019applicazione e dal set-up specifico della strumentazione. Per l\u2019analisi dei composti polari con capacit\u00e0 di chelazione nota (come il glifosato), Restek consiglia di trattare il sistema con una soluzione passivante per LC (cat.#\u00a032475) prima di eseguire le analisi dei campioni.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">La qualit\u00e0 Raptor resiste alle prove del tempo e d\u00e0&nbsp;vita a una nuova fase stazionaria<\/h2>\n\n\n\n<p>La colonna Raptor Polar X \u00e8 stata progettata, realizzata e testata per rispettare gli stessi rigorosi standard che rendono le colonne Raptor sinonimo di qualit\u00e0. Puoi sempre contare sul marchio di qualit\u00e0 Raptor: avrai prestazioni stabili un\u2019iniezione dopo l\u2019altra nell\u2019analisi di campioni reali in matrici complesse come gli spinaci (Figura 8), e manterrai la stessa affidabilit\u00e0 colonna dopo colonna, quando dovrai sostituirla (Figura 9). Inoltre, l\u2019adozione di un singolo ligando per la ritenzione ibrida della colonna Raptor Polar X garantisce che ogni colonna sia realizzata in modo uniforme e sia in grado di sopportare anche condizioni aggressive, come le iniezioni di grandi volumi d\u2019acqua, senza alcuna perdita di fase stazionaria.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 8:<\/strong>\u00a0L\u2019eccellente forma del picco e la stabilit\u00e0 del tempo di ritenzione dopo centinaia di iniezioni di un estratto di spinaci dimostrano l\u2019eccellenza delle prestazioni ottenute con le colonne Raptor Polar X per l\u2019analisi dei composti polari.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figura 9:<\/strong>\u00a0Le colonne Raptor Polar X garantiscono un\u2019affidabile riproducibilit\u00e0 fra lotti diversi.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.png\" alt=\"Lot-to-Lot Comparison: Polar Pesticides in Solvent on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0543<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0543\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Colonne Raptor Polar X: rivoluziona l\u2019analisi LC dei composti polari<\/h2>\n\n\n\n<p>Scarsa ritenzione, bassa risposta e procedure di preparazione dei campioni complesse&#8230; l\u2019analisi dei composti polari pone da sempre delle difficolt\u00e0 per quanto riguarda la qualit\u00e0 dei dati e la produttivit\u00e0 del laboratorio. Ora la colonna Raptor Polar X, con l\u2019innovativa fase che unisce la ritenzione HILIC e quella a scambio ionico in un unico ligando, semplifica notevolmente l\u2019analisi di questi composti difficili e variegati. Inoltre, attraverso il legame di quell\u2019unico ligando alle particelle superficialmente porose (SPP), Restek ha creato una fase che trattiene e separa efficientemente una vasta gamma di composti polari. La sinergia creata da queste caratteristiche d\u00e0 origine a una capacit\u00e0 senza precedenti di analizzare una vasta gamma di composti polari, fornendo agli scienziati che eseguono analisi LC in svariati settori uno strumento potente, performante e versatile.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">References<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Bibliografia<\/li>\n\n\n\n<li>M. Anastassiades, A.-K. Wachtler, D. I. Kolberg, E. Eichhorn, H. Marks, A. Benkenstein, S. Zechmann; D. Mack, C. Wildgrube, A. Barth, I. Sigalov, S. G\u00f6rlich, D. D\u00f6rk, G. Cerchia, Quick method for the analysis of highly polar pesticides in food involving extraction with acidified methanol and LC &#8211; or ICMS\/MS Measurement &#8211; I. Food of Plant Origin (QuPPe-PO-Method)\u2013Version 12 (published on EURL-SRM website on July 23, 2021).\u00a0<a href=\"https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">                                Catalog No. 32475                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Soluzione passivante per LC, acido metilendifosfonico (acido medronico), 1760 \u00b5g\/mL, metanolo (grado HPLC)\/acqua (50:50), 1 mL\/ampolla<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/32475\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">                                Catalog No. 9311A32                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonna LC Raptor Polar X, 2,7um, 30&#215;2,1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A12\">                                Catalog No. 9311A12                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Colonna LC Raptor Polar X, 2,7um, 100&#215;2,1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A12\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">                                Catalog No. 9311A0252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Cartuccia per precolonna Raptor Polar X EXP, 2,7um, 5&#215;2,1mm<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">                                Catalog No. 25846                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Provette da centrifuga vuote da 50 ml, Polipropilene con tappo, 50 provette, 50-conf.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">                                Catalog No. 23984                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Filtro siringa da 30 mm, 0,22 \u00b5m, PTFE, bianco, 100 pz.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">Esplora Prodotto<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>L&#8217;esclusiva fase ibrida delle colonne Raptor Polar X bilancia le modalit\u00e0 di ritenzione HILIC e a scambio ionico, rendendole ideali per l&#8217;analisi di un&#8217;ampia gamma di composti polari, soprattutto se accoppiate alla spettrometria di 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