As Applications Chemists in the LC lab, one of the most exciting parts of our jobs is the variety of analyses we are exposed to. One day you are developing a method for potency analysis in cannabis samples, the next you are looking at anti-epileptic drugs in urine. We\u2019re regularly challenged to think outside the box to provide the best solutions for our customers.<\/p>\n\n\n\n
In some recent work, my colleague, Shun-Hsin Liang, was tasked to develop a method for nicotine and related compounds in urine using LC-MS\/MS. The challenge was finding suitable analytical conditions for a rapid, accurate, and reproducible method geared toward high-throughput testing laboratories. Typically, this analysis uses high-pH chromatography with relatively high concentrations of additives to increase retention, improve peak shape, and reduce peak tailing. These conditions may lead to some additional non-routine maintenance (like changing pump seals and replacing tubing), and if you\u2019re in a high-throughput laboratory, you know non-routine maintenance events always pop up at the most inopportune time.<\/p>\n\n\n\n
Shun-Hsin was able to use the Raptor Biphenyl column and standard, low pH, reversed-phase LC-MS mobile phases that are compatible with a variety of LC-MS instrumentation. The method provided excellent performance for the simultaneous analysis of nicotine, two major metabolites (cotinine and trans<\/em>-3\u2019-hydroxycotinine), two minor metabolites (nornicotine and norcotinine), and a minor tobacco alkaloid, anabasine, in human urine. Accurate and reproducible analysis was achieved in less than 5 minutes of chromatographic analysis time, making the column and method well suited to low-cost, high-throughput analysis of nicotine-related compounds.<\/p>\n\n\n\n As Applications Chemists in the LC lab, one of the most exciting parts of our jobs is the variety of analyses we are exposed to. One day you are developing a method for potency analysis in cannabis samples, the next…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2153,2154,2155,2156],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2299],"ppma_author":[586],"class_list":["post-51604","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-clinical","industries-application-clinical-diagnostics","industries-application-clinical-research","industries-application-clinical-toxicology","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2153,"label":"Clinical"},{"value":2154,"label":"Clinical Diagnostics"},{"value":2155,"label":"Clinical Research"},{"value":2156,"label":"Clinical Toxicology"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":false,"author_info":{"display_name":"DRCadmin","author_link":"https:\/\/discover.restek.com\/it\/author\/drcadmin\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":444,"filter":"raw","cat_ID":9,"category_count":444,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":586,"user_id":0,"is_guest":1,"slug":"frances-carroll","display_name":"Frances Carroll","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51604","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=51604"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51604\/revisions"}],"predecessor-version":[{"id":58670,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51604\/revisions\/58670"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=51604"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=51604"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=51604"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=51604"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=51604"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=51604"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=51604"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=51604"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=51604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}