{"id":51643,"date":"2022-08-14T14:30:00","date_gmt":"2022-08-14T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/chiral-separations-8-essential-oils-and-fragrance-on-rt-%ce%b2dexsa\/"},"modified":"2026-01-28T15:50:56","modified_gmt":"2026-01-28T15:50:56","slug":"chiral-separations-8-essential-oils-and-fragrance-on-rt-bdexsa","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/blogs\/gnbl4625\/chiral-separations-8-essential-oils-and-fragrance-on-rt-bdexsa","title":{"rendered":"Chiral Separations 8: Essential oils and fragrance on Rt-\u03b2DEXsa"},"content":{"rendered":"\n
\"bottles<\/figure>\n\n\n\n

In this blog we come back to the Rt-\u03b2DEXsa column and its application to rose oils and fragrance. The Rt-bDEXsa has a significantly different selectivity than the other chiral columns. It provides the best separation of 1-octen-3-ol, carvone, camphor, b-citronellol, and rose oxides, which makes it a good fit for certain essential oils\u2019 analysis. We\u2019ll look at geranium oil, pure rose oil and rose fragrance.<\/p>\n\n\n\n

Geranium oil<\/h2>\n\n\n\n

Geranium is a plant from Rosids clade, thus its oil\u2019s composition is similar to the rose oil. Chiral constituents in geranium oils include cis and trans rose oxides, menthone, linalool, and \u03b2-citronellol. The Rt- \u03b2DEXsa column provides chiral resolution for all of these compounds. In authentic samples of geranium oil, (-)-(4R)-configured diastereomers of cis<\/em>– and trans<\/em>-rose oxides predominate over their (+)-enantiomers. The (-)-(S) form of \uf062-citronellol should be 74-80% of the enantiomeric ratio. Note that cis-<\/em> and trans-<\/em>rose oxides and \u03b2-citronellol are racemic in this particular commercial geranium oil, as shown in Figure 1. These racemic compounds indicate that this oil is not authentic.<\/p>\n\n\n\n

Figure 1: Geranium oil on Rt-bDEXsa.<\/em><\/p>\n\n\n

\"Geranium<\/div>

GC_FF1372<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> 1<\/th>tR<\/sub> 2<\/th>tR<\/sub> 3<\/th>tR<\/sub> 4<\/th><\/tr><\/thead>\n
1.<\/td>Rose oxides<\/a><\/td>22.701<\/td>22.931<\/td>23.370<\/td>23.616<\/td><\/tr>\n
2.<\/td>(-) Menthone<\/a><\/td>28.420<\/td>–<\/td>–<\/td>–<\/td><\/tr>\n
3.<\/td>Linalool (\u00b1)<\/a><\/td>30.919<\/td>31.298<\/td>–<\/td>–<\/td><\/tr>\n
4.<\/td>\u03b2-Citronellol (\u00b1)<\/a><\/td>37.090<\/td>37.245<\/td>–<\/td>–<\/td><\/tr>\n
5.<\/td>Geraniol<\/a><\/td>39.494<\/td>–<\/td>–<\/td>–<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n

Rose oil and rose fragrance<\/h2>\n\n\n\n

As geranium oil, rose oil also contains rose oxides and \u03b2-citronellol (Fig 2). Menthone is present as well, however in a lower amount. In this sample of rose oil, the rose oxides were also in much lower amount than in the geranium and all four isomers were present. The presence of all rose oxides with together with equally abundant \u03b2-citronellol enantiomers suggest that this sample is not authentic.<\/p>\n\n\n\n

Figure 2: Rose oil on Rt-bDEXsa<\/em><\/p>\n\n\n

\"Rose<\/div>

GC_FF1373<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> 1<\/th>tR<\/sub> 2<\/th>tR<\/sub> 3<\/th>tR<\/sub> 4<\/th><\/tr><\/thead>\n
1.<\/td>Rose oxides<\/a><\/td>22.700<\/td>22.920<\/td>23.360<\/td>23.610<\/td><\/tr>\n
2.<\/td>(-) Menthone<\/a><\/td>28.416<\/td>–<\/td>–<\/td>–<\/td><\/tr>\n
3.<\/td>\u03b2-Citronellol (\u00b1)<\/a><\/td>37.066<\/td>37.230<\/td>–<\/td>–<\/td><\/tr>\n
4.<\/td>Geraniol<\/a><\/td>39.805<\/td>–<\/td>–<\/td>–<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n

The rose fragrance had some of the typical components of the rose oil, however, the rose oxides were completely missing (Figure 3).<\/p>\n\n\n\n

Figure 3: Rose fragrance on Rt-bDEXsa<\/em><\/p>\n\n\n

\"Rose<\/div>

GC_FF1375<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> 1<\/th>tR<\/sub> 2<\/th><\/tr><\/thead>\n
1.<\/td>Menthone<\/a><\/td>28.416<\/td>–<\/td><\/tr>\n
2.<\/td>\u03b2-Citronellol (\u00b1)<\/a><\/td>37.066<\/td>37.230<\/td><\/tr>\n
3.<\/td>Geraniol<\/a><\/td>39.805<\/td>–<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n

This concludes our series on chiral chromatography. If you have any suggestions on expanding the scope, please let us know!<\/p>\n\n\n\n

<\/div>\n\n\n
\n

List of our chiral column offerings:<\/h3>\n
\n
\n
\n
\n Catalog No. 13108 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-bDEXsa 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13109 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-bDEXsa 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13102 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-bDEXcst 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13103 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-bDEXcst 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13100 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXm 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13101 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXm 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13106 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXse 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13107 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXse 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13104 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXsm 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13105 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-\u03b2DEXsm 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13112 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-gamma DEXsa 30 m, ID 0,32 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 13113 <\/a> <\/div>\n
\n
Colonna capillare GC Chiral, Rt-gamma DEXsa 30 m, ID 0,25 mm, 0,25 \u00b5m<\/div>\n <\/div>\n
\n Esplora Prodotto<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

In this blog we come back to the Rt-βDEXsa column and its application to rose oils and fragrance. The Rt-bDEXsa has a significantly different selectivity than the other chiral columns. It provides the best separation of 1-octen-3-ol, carvone, camphor, b-citronellol, and rose oxides, which makes it a good fit for certain essential oils’ analysis. We’ll look at geranium oil, pure rose oil and rose fragrance.<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2149,2167],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2294],"ppma_author":[591],"class_list":["post-51643","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-cannabis","industries-application-food-beverage","resource-technique-gas-chromatography-gc"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2149,"label":"Cannabis"},{"value":2167,"label":"Food & Beverage"}],"resource-technique":[{"value":2294,"label":"Gas Chromatography (GC)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jana Hepner, PhD","author_link":"https:\/\/discover.restek.com\/it\/author\/jana-hepner-phd\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":9,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":591,"user_id":50,"is_guest":0,"slug":"jana-hepner-phd","display_name":"Jana Hepner, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51643","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=51643"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51643\/revisions"}],"predecessor-version":[{"id":85259,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51643\/revisions\/85259"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=51643"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=51643"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=51643"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=51643"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=51643"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=51643"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=51643"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=51643"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=51643"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}