{"id":51748,"date":"2023-09-07T00:00:00","date_gmt":"2023-09-07T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/more-than-you-ever-wanted-to-know-about-calibrations-series-index\/"},"modified":"2025-12-10T22:31:42","modified_gmt":"2025-12-10T22:31:42","slug":"more-than-you-ever-wanted-to-know-about-calibrations-series-index","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/blog-it\/gnbl4740\/more-than-you-ever-wanted-to-know-about-calibrations-series-index","title":{"rendered":"More Than You Ever Wanted to Know About Calibrations &#8211; Series Index"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Part 1 &#8211; Types of Calibrations<\/h2>\n\n\n\n<p>In previous blog posts on TO-15A and OTM-45 I\u2019ve expressed strong opinions about calibration acceptance criteria. While the acceptance criteria is important, I thought I\u2019d step back and delve more deeply into calibrations, starting with the different types of calibration. A calibration is just a way to convert your instrument response to a known of analyte, and then to a concentration based on injection amount and sample preparation steps, but there are several different flavors of calibration to choose from, and choosing the best one requires you to weigh the advantages and disadvantages. The following post will highlight some of those differences, therefore hopefully enabling you to make the best educated decision for your lab.&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\nhttps:\/\/discover.restek.com\/GNBL4741\/more-than-you-ever-wanted-to-know-about-calibrations-part-1-types-of-calibrations\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 2 &#8211; Curve Fits and Weighting<\/h2>\n\n\n\n<p>Once you\u2019ve determined that, the next step is how to mathematically go from a response reading to a concentration. Be warned, this blog will be a bit math heavy.&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"s65Q8Yqxos\"><a href=\"https:\/\/discover.restek.com\/GNBL4742\/more-than-you-ever-wanted-to-know-about-calibrations-part-2-curve-fits-and-weighting\/\">More Than You Ever Wanted to Know About Calibrations, Part 2 &ndash; Curve Fits and Weighting<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 2 &ndash; Curve Fits and Weighting&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4742\/more-than-you-ever-wanted-to-know-about-calibrations-part-2-curve-fits-and-weighting\/embed\/#?secret=cwJ9L5iIyB#?secret=s65Q8Yqxos\" data-secret=\"s65Q8Yqxos\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 3 &#8211; Dealing with Zero Points<\/h2>\n\n\n\n<p>In this installment I\u2019ll cover issues around the calibration zero point. This is a topic that many methods give little guidance on, and various methods can handle it very differently; so it\u2019s easy to be confused over what best practices are, and hopefully I can clear up some confusion. &nbsp;When I talk about zero points in calibrations, there are two different things to consider. First, whether a zero point or blank needs to be included in the calibration, and second, whether a calibration curve should be forced through zero. I\u2019ll go over how these might affect your calibration before discussing some examples of how various EPA methods handle them.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"CHnfzDjAiL\"><a href=\"https:\/\/discover.restek.com\/GNBL4744\/more-than-you-ever-wanted-to-know-about-calibrations-part-3-dealing-with-zero-points\/\">More Than You Ever Wanted to Know About Calibrations, Part 3 &ndash; Dealing with Zero Points<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 3 &ndash; Dealing with Zero Points&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4744\/more-than-you-ever-wanted-to-know-about-calibrations-part-3-dealing-with-zero-points\/embed\/#?secret=1E4glQ89RN#?secret=CHnfzDjAiL\" data-secret=\"CHnfzDjAiL\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 4 &#8211; Calibration Acceptance<\/h2>\n\n\n\n<p>In this post we\u2019ll cover how to tell if your calibration is accurate. The previous posts have been a bit dense and math heavy, but the TL;DR on this one is pretty simple: r2 is bad and you shouldn\u2019t use it.&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"LXoKuc6kpn\"><a href=\"https:\/\/discover.restek.com\/GNBL4745\/more-than-you-ever-wanted-to-know-about-calibrations-part-4-calibration-acceptance\/\">More Than You Ever Wanted to Know About Calibrations, Part 4 &ndash; Calibration Acceptance<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 4 &ndash; Calibration Acceptance&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4745\/more-than-you-ever-wanted-to-know-about-calibrations-part-4-calibration-acceptance\/embed\/#?secret=x4i0g2s9dE#?secret=LXoKuc6kpn\" data-secret=\"LXoKuc6kpn\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 5 &#8211; Accuracy, Precision and Detection Limits<\/h2>\n\n\n\n<p>In this post we\u2019ll talk about how calibrations affect your results, focusing especially on low-level accuracy, precision, and detection limits.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"8Zi5QkUHQm\"><a href=\"https:\/\/discover.restek.com\/GNBL4746\/more-than-you-ever-wanted-to-know-about-calibrations-part-5-accuracy-precision-and-detection-limits\/\">More Than You Ever Wanted to Know About Calibrations, Part 5 &ndash; Accuracy, Precision and Detection Limits<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 5 &ndash; Accuracy, Precision and Detection Limits&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4746\/more-than-you-ever-wanted-to-know-about-calibrations-part-5-accuracy-precision-and-detection-limits\/embed\/#?secret=CnLGOF89kJ#?secret=8Zi5QkUHQm\" data-secret=\"8Zi5QkUHQm\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 6 &#8211; Linearity and Calibration Spacing<\/h2>\n\n\n\n<p>Starting with this post we\u2019ll be looking more at the practical considerations of how to make a calibration, starting with how to choose your calibration points.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"aCAAtbTcit\"><a href=\"https:\/\/discover.restek.com\/GNBL4747\/more-than-you-ever-wanted-to-know-about-calibrations-part-6-linearity-and-calibration-spacing\/\">More Than You Ever Wanted to Know About Calibrations, Part 6 &ndash; Linearity and Calibration Spacing<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 6 &ndash; Linearity and Calibration Spacing&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4747\/more-than-you-ever-wanted-to-know-about-calibrations-part-6-linearity-and-calibration-spacing\/embed\/#?secret=OjkgfEF9Ts#?secret=aCAAtbTcit\" data-secret=\"aCAAtbTcit\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 6 (Continued) \u2013 Even More On Calibration Spacing<\/h2>\n\n\n\n<p>In this post we delve deeper into calibration spacing and revisit some of the assumptions we made in the previous post.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"q84GE9Xf8d\"><a href=\"https:\/\/discover.restek.com\/GNBL4748\/more-than-you-ever-wanted-to-know-about-calibrations-part-6-continued-even-more-on-calibration-spacing\/\">More Than You Ever Wanted to Know About Calibrations, Part 6 (Continued) &ndash; Even More On Calibration Spacing<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 6 (Continued) &ndash; Even More On Calibration Spacing&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4748\/more-than-you-ever-wanted-to-know-about-calibrations-part-6-continued-even-more-on-calibration-spacing\/embed\/#?secret=tpH6PBXJ5E#?secret=q84GE9Xf8d\" data-secret=\"q84GE9Xf8d\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 7 \u2013 Internal Standards, Surrogates, and Isotope Dilution<\/h2>\n\n\n\n<p>In this post of our calibration series, we demystify the differences between extracted internal standards and surrogates.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-provider-wordpress wp-block-embed-wordpress\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"hvExhogAUg\"><a href=\"https:\/\/discover.restek.com\/GNBL4743\/more-than-you-ever-wanted-to-know-about-calibrations-part-7-internal-standards-surrogates-and-isotope-dilution\/\">More Than You Ever Wanted to Know About Calibration, Part 7 &ndash; Internal Standards, Surrogates, and Isotope Dilution<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibration, Part 7 &ndash; Internal Standards, Surrogates, and Isotope Dilution&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4743\/more-than-you-ever-wanted-to-know-about-calibrations-part-7-internal-standards-surrogates-and-isotope-dilution\/embed\/#?secret=JzCqXkpgRk#?secret=hvExhogAUg\" data-secret=\"hvExhogAUg\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Part 8 \u2013 Low Level Replicates<\/h2>\n\n\n\n<p>Does changing from duplicate to triplicate (or from triplicate to quadruplicate) determination in the highest variation region improve the calibration performance? Find out in this blog post!<\/p>\n\n\n\n<figure class=\"wp-block-embed\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"WIg35PbGr4\"><a href=\"https:\/\/discover.restek.com\/GNBL4924\/more-than-you-ever-wanted-to-know-about-calibrations-part-8-low-level-replicates\/\">More Than You Ever Wanted to Know About Calibrations, Part 8 \u2013 Low Level Replicates<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;More Than You Ever Wanted to Know About Calibrations, Part 8 \u2013 Low Level Replicates&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/GNBL4924\/more-than-you-ever-wanted-to-know-about-calibrations-part-8-low-level-replicates\/embed\/#?secret=sIDmgslS2z#?secret=WIg35PbGr4\" data-secret=\"WIg35PbGr4\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Part 1 &#8211; Types of Calibrations In previous blog posts on TO-15A and OTM-45 I\u2019ve expressed strong opinions about calibration acceptance criteria. While the acceptance criteria is important, I thought I\u2019d step back and delve more deeply into calibrations, starting with the different types of calibration. A calibration is just a way to convert your&#8230;<\/p>\n","protected":false},"author":27,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[793],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2294,2299],"hf_cat_post":[623],"ppma_author":[447],"class_list":["post-51748","post","type-post","status-publish","format-standard","hentry","category-blog-it","resource-technique-gas-chromatography-gc","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":793,"label":"Blog"}],"resource-technique":[{"value":2294,"label":"Gas Chromatography (GC)"},{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jason Hoisington","author_link":"https:\/\/discover.restek.com\/it\/author\/jason-hoisington\/"},"comment_info":0,"category_info":[{"term_id":793,"name":"Blog","slug":"blog-it","term_group":0,"term_taxonomy_id":793,"taxonomy":"category","description":"","parent":0,"count":442,"filter":"raw","cat_ID":793,"category_count":442,"category_description":"","cat_name":"Blog","category_nicename":"blog-it","category_parent":0}],"tag_info":false,"authors":[{"term_id":447,"user_id":27,"is_guest":0,"slug":"jason-hoisington","display_name":"Jason Hoisington","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hoisington-jason-01.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hoisington-jason-01.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51748","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=51748"}],"version-history":[{"count":9,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51748\/revisions"}],"predecessor-version":[{"id":73314,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/51748\/revisions\/73314"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=51748"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=51748"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=51748"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=51748"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=51748"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=51748"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=51748"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=51748"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/hf_cat_post?post=51748"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=51748"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}