{"id":76402,"date":"2022-07-27T00:00:00","date_gmt":"2022-07-27T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76402"},"modified":"2026-01-28T15:50:16","modified_gmt":"2026-01-28T15:50:16","slug":"beer-volatiles-analysis-part-1","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/blog-fr\/gnbl5471\/beer-volatiles-analysis-part-1","title":{"rendered":"Beer Volatiles Analysis \u2013 Part 1"},"content":{"rendered":"<style>.kb-image76402_af5b3d-da .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image76402_af5b3d-da size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"214\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-01-1024x214.jpg\" alt=\"\" class=\"kb-img wp-image-77156\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-01-1024x214.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-01-300x63.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-01-768x161.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-01.jpg 1253w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Over the past year, I have had the opportunity to work with some great breweries. In those conversations, I came across a consistent issue; the analysis of Beer Volatiles represents a significant hurdle due to sample complexity. After joining the American Society of Brewing Chemists (ASBC), I realized this is a critical test in brew labs&nbsp;[1]. We\u2019re here to help, hence we decided to start this series.<\/p>\n\n\n\n<p>Our first challenge is proper sample preparation and chromatographic separation. Since these compounds of interest are volatile (see Table 1), they will not be retained well on a column with a thin film. Additionally, these compounds are polar, so it is important to make sure the correct stationary phase is selected.\u00a0 So, how do we find a column with the right polarity and column dimensions? Easy! We go to the\u00a0<a href=\"https:\/\/ez.restek.com\/proezgc\/\" data-type=\"link\" data-id=\"https:\/\/ez.restek.com\/proezgc\/\" target=\"_blank\" rel=\"noopener\">Restek ProEZGC Chromatogram Modeler<\/a>!<\/p>\n\n\n<p>Table 1: Beer Volatiles<\/p>\n<figure class=\"wp-block-table table table-bordered\">\n<table style=\"border-style: none;\">\n<tbody>\n<tr>\n<td>\n<p><strong>#<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Compound<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>1<\/strong><\/p>\n<\/td>\n<td>\n<p>Acetaldehyde<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>2<\/strong><\/p>\n<\/td>\n<td>\n<p>Ethanol<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>3<\/strong><\/p>\n<\/td>\n<td>\n<p>Ethyl Acetate<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>4<\/strong><\/p>\n<\/td>\n<td>\n<p>n-Butanol<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>5<\/strong><\/p>\n<\/td>\n<td>\n<p>Isoamyl Alcohol<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>6<\/strong><\/p>\n<\/td>\n<td>\n<p>Isoamyl Acetate<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<p>After adding our compounds of interest into the compound table, we hit \u201csolve,\u201d and are given several column options. These include the Rtx-502.2, Stabilwax, and Rxi-624Sil MS. Out of these, I recommend the Rxi-624Sil MS for its selectivity, inertness, low bleed, and compatibility with the mass spec. In ProEZGC, you can select column dimensions and change the method parameters to improve the chromatography to your liking. After selecting the 30m x 0.25mm x 1.4\u00b5m dimension, I did some method optimization and came up with the method shown below.<\/p>\n\n\n<style>.kb-image76402_014f74-8e .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image76402_014f74-8e size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"404\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-02-1024x404.png\" alt=\"\" class=\"kb-img wp-image-77142\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-02-1024x404.png 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-02-300x118.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-02-768x303.png 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-beer-volatiles-analysis-part-1-02.png 1213w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>As you can see, the modeled chromatogram for this method looks pretty good! We have great resolution between acetaldehyde and ethanol, and the remaining compounds of interest are separated. ProEZGC Chromatogram modeler is a great tool for starting a new application! Stay tuned for ProEZGC to be put to the test with some real beer samples! Of course, that is assuming the samples make it safely to the lab\u2026 &nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">References<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>American Society of Brewing Chemists, &#8220;HEADSPACE GAS CHROMATOGRAPHY\u2013FLAME IONIZATION DETECTION ANALYSIS OF BEER VOLATILES (Beer-48),&#8221; pp. 1-4, 2012.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Over the past year, I have had the opportunity to work with some great breweries. In those conversations, I came across a consistent issue; the analysis of Beer Volatiles represents a significant hurdle due to sample complexity. After joining the American Society of Brewing Chemists (ASBC), I realized this is a critical test in brew labs [1]. We&rsquo;re here to help, hence we decided to start this series.<\/p>\n","protected":false},"author":32,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[791],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[623],"ppma_author":[452],"class_list":["post-76402","post","type-post","status-publish","format-standard","hentry","category-blog-fr"],"acf":[],"taxonomy_info":{"category":[{"value":791,"label":"Blog"}]},"featured_image_src_large":false,"author_info":{"display_name":"Colton Myers","author_link":"https:\/\/discover.restek.com\/it\/author\/colton-myers\/"},"comment_info":0,"category_info":[{"term_id":791,"name":"Blog","slug":"blog-fr","term_group":0,"term_taxonomy_id":791,"taxonomy":"category","description":"","parent":0,"count":442,"filter":"raw","cat_ID":791,"category_count":442,"category_description":"","cat_name":"Blog","category_nicename":"blog-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":452,"user_id":32,"is_guest":0,"slug":"colton-myers","display_name":"Colton Myers","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-myers-colton.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-myers-colton.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76402","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/32"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=76402"}],"version-history":[{"count":7,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76402\/revisions"}],"predecessor-version":[{"id":85242,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76402\/revisions\/85242"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=76402"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=76402"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=76402"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=76402"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=76402"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=76402"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=76402"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=76402"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/hf_cat_post?post=76402"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=76402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}