{"id":76444,"date":"2022-05-25T00:00:00","date_gmt":"2022-05-25T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76444"},"modified":"2025-12-26T19:17:06","modified_gmt":"2025-12-26T19:17:06","slug":"new-ezgc-library-residual-solvents-on-the-rtx-225","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/blogs\/gnbl5520\/new-ezgc-library-residual-solvents-on-the-rtx-225","title":{"rendered":"New EZGC library: Residual solvents on the Rtx-225"},"content":{"rendered":"\n<p>The Rtx-225 is a versatile column with unique selectivity for low-boiling-point compounds. &nbsp;The cyano-phenyl composition of this phase limits the maximum operating temperature to &lt;240\u00b0C.<\/p>\n\n\n\n<p>To help figure out if this column will work for your analytes of interest, a new residual solvents library has been added to EZGC, containing compounds from all three solvent classes designated by the USP and ICH&nbsp;<sup>1,2<\/sup>&nbsp;(Table 1).<\/p>\n\n\n\n<p><strong>Table 1:<\/strong>&nbsp;Residual solvents included in the Rtx-225 EZGC library.<\/p>\n\n\n\n<figure class=\"wp-block-table table\"><table><tbody><tr><td>\n<p>Chemical:<\/p>\n<\/td><td>\n<p>CAS (#)<\/p>\n<\/td><td>\n<p>Class (#)<\/p>\n<\/td><\/tr><tr><td>\n<p>Benzene<\/p>\n<\/td><td>\n<p>71-43-2<\/p>\n<\/td><td>\n<p>1<\/p>\n<\/td><\/tr><tr><td>\n<p>Chloroform<\/p>\n<\/td><td>\n<p>67-66-3<\/p>\n<\/td><td>\n<p>1<\/p>\n<\/td><\/tr><tr><td>\n<p>Trichloroethene<\/p>\n<\/td><td>\n<p>79-01-6<\/p>\n<\/td><td>\n<p>1<\/p>\n<\/td><\/tr><tr><td>\n<p>1,4-Dioxane<\/p>\n<\/td><td>\n<p>123-91-1<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Chlorobenzene<\/p>\n<\/td><td>\n<p>108-90-7<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>cis-1,2-Dichloroethene (Z)<\/p>\n<\/td><td>\n<p>156-59-2<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Cyclohexane<\/p>\n<\/td><td>\n<p>110-82-7<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Ethylbenzene<\/p>\n<\/td><td>\n<p>100-41-4<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>m\/p-Xylene<\/p>\n<\/td><td>\n<p>108-38-3<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Methylcyclohexane<\/p>\n<\/td><td>\n<p>108-87-2<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>o-Xylene<\/p>\n<\/td><td>\n<p>95-47-6<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Tetrahydrofuran<\/p>\n<\/td><td>\n<p>109-99-9<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>Toluene<\/p>\n<\/td><td>\n<p>108-88-3<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>trans-1,2-Dichloroethene (E)<\/p>\n<\/td><td>\n<p>156-60-5<\/p>\n<\/td><td>\n<p>2<\/p>\n<\/td><\/tr><tr><td>\n<p>1-Butanol<\/p>\n<\/td><td>\n<p>71-36-3<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>1-Pentanol<\/p>\n<\/td><td>\n<p>71-41-0<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>1-Propanol<\/p>\n<\/td><td>\n<p>71-23-8<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>2-Butanol (sec-butyl alcohol)<\/p>\n<\/td><td>\n<p>78-92-2<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>2-Butanone (MEK)<\/p>\n<\/td><td>\n<p>78-93-3<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>2-Propanol (isopropanol)<\/p>\n<\/td><td>\n<p>67-63-0<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>3-Methyl-1-butanol (isoamyl alcohol)<\/p>\n<\/td><td>\n<p>123-51-3<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>4-Methyl-2-pentanone (MIBK)<\/p>\n<\/td><td>\n<p>108-10-1<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Butyl acetate<\/p>\n<\/td><td>\n<p>123-86-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Diethyl ether (ethyl ether)<\/p>\n<\/td><td>\n<p>60-29-7<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Ethanol<\/p>\n<\/td><td>\n<p>64-17-5<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Ethyl acetate<\/p>\n<\/td><td>\n<p>141-78-6<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Ethyl formate<\/p>\n<\/td><td>\n<p>109-94-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Isobutanol (2-methyl-1-propanol)<\/p>\n<\/td><td>\n<p>78-83-1<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Isobutyl acetate (acetic acid 2-methylpropyl ester)<\/p>\n<\/td><td>\n<p>108-21-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Isopropyl acetate (acetic acid, 1-methylethyl ester)<\/p>\n<\/td><td>\n<p>108-21-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Methyl acetate<\/p>\n<\/td><td>\n<p>79-20-9<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Methyl-tert-butyl ether (MTBE)<\/p>\n<\/td><td>\n<p>1634-04-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><tr><td>\n<p>Propyl acetate<\/p>\n<\/td><td>\n<p>109-60-4<\/p>\n<\/td><td>\n<p>3<\/p>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>Using EZGC, we were able to build a method that could separate 25 of the 33 compounds in under 5 minutes (Figure 1).<\/p>\n\n\n\n<p><strong>Figure 1<\/strong>: EZGC method predictions for separation of class 1-3 residual solvents.<\/p>\n\n\n<style>.kb-image76444_44a08a-af:not(.kb-image-is-ratio-size) .kb-img, .kb-image76444_44a08a-af.kb-image-is-ratio-size{padding-top:var(--global-kb-spacing-xs, 1rem);padding-bottom:var(--global-kb-spacing-xs, 1rem);}.kb-image76444_44a08a-af .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image76444_44a08a-af size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"678\" height=\"1272\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-new-ezgc-library-residual-solvents-on-the-rtx-225-01.png\" alt=\"\" class=\"kb-img wp-image-78385\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-new-ezgc-library-residual-solvents-on-the-rtx-225-01.png 678w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-new-ezgc-library-residual-solvents-on-the-rtx-225-01-160x300.png 160w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-new-ezgc-library-residual-solvents-on-the-rtx-225-01-546x1024.png 546w\" sizes=\"auto, (max-width: 678px) 100vw, 678px\" \/><\/figure>\n\n\n\n<p>Our column was measured to be 29.09m. We used an inlet temperature of 250<sup>o<\/sup>C, Topaz straight inlet liner with wool (cat#23300), and a 400:1 split injection. The standards used were cat# 36004 (class 1), 36217 (class 2), 36013 (class 3).<\/p>\n\n\n\n<p>Even though we had quite a few coelutions, the use of a mass-spectrometers helped to identify individual peaks for all 33 compounds. If you are also using a mass-spectrometer, the EZGC chromatogram modeler can help you to select unique ions differentiating co-eluting peaks, saving valuable trial-and-error time.<\/p>\n\n\n\n<p><strong>For further reading:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Impurities: Guideline for Residual Solvents Q3C(R8). International council for harmonisation of technical requirements for pharmaceuticals for human use April 22, 2021.<\/li>\n\n\n\n<li>&lt;467> RESIDUAL SOLVENTS. United States Pharmacopia March 23, 2007.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Rtx-225 is a versatile column with unique selectivity low-boiling-point compounds. &nbsp;The cyano-phenyl composition of this phase limits the maximum operating temperature to &lt;240&deg;C.&nbsp;<br \/>To help figure out if this column will work for your analytes of interest, a new residual solvents library has been added to EZGC, containing compounds from all three solvent classes designated by the USP and ICH 1,2.<\/p>\n","protected":false},"author":34,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[454],"class_list":["post-76444","post","type-post","status-publish","format-standard","hentry","category-blogs"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}]},"featured_image_src_large":false,"author_info":{"display_name":"Erica Pack, PhD","author_link":"https:\/\/discover.restek.com\/it\/author\/erica-pack-phd\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":9,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":454,"user_id":34,"is_guest":0,"slug":"erica-pack-phd","display_name":"Erica Pack, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-pack-erica.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-pack-erica.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76444","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/34"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=76444"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76444\/revisions"}],"predecessor-version":[{"id":78396,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/76444\/revisions\/78396"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=76444"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=76444"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=76444"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=76444"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=76444"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=76444"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=76444"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=76444"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=76444"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}