{"id":84076,"date":"2026-01-22T21:18:04","date_gmt":"2026-01-22T21:18:04","guid":{"rendered":"https:\/\/discover.restek.com\/?p=84076"},"modified":"2026-01-22T21:18:08","modified_gmt":"2026-01-22T21:18:08","slug":"one-column-one-setup-consolidating-lc-ms-ms-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/it\/posters-presentations-it\/gnot5374\/one-column-one-setup-consolidating-lc-ms-ms-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse","title":{"rendered":"One Column, One Setup: Consolidating LC-MS\/MS Method Conditions for the Analysis of Alcohol Metabolites, Barbiturates, and Drugs of Abuse"},"content":{"rendered":"\n<p>A panel of 136 ESI+\/ESI- therapeutic drugs; drugs of abuse and their metabolites; and biomarkers of alcohol consumption were analyzed using a Force Biphenyl analytical column equipped with a guard column and an online filter. Mobile phase A consisted of 0.1% formic acid in water, mobile phase B consisted of 0.1% formic acid in methanol, the temperature was 30\u00b0C, and the ESI+ isobars utilized gradient conditions with a 10 minute cycle time. Urine samples underwent glucuronide hydrolysis. Barbiturates, THCA-A, and THC-COOH were analyzed in ESI- and had a total run time of 5 minutes. They were spiked into urine and diluted at a 1:10 ratio with water. Finally, alcohol metabolites were monitored in ESI- with a 5 minute analysis time. Samples were prepared by diluting with water at a 1:10 ratio and injecting 10 \u03bcL.<\/p>\n\n\n\n<p>The Biphenyl stationary phase showed an improved resolution of isobars thanks to its unique selectivity due to the pi-pi interactions that occur between the phase and most drugs and drug metabolites. A demonstration of this powerful selectivity is exemplified for seven isobaric compounds sharing the m\/z 286 (morphine, hydromorphone, norcodeine, norhydrocodone, 7-aminoclonazepam, pentazocine, and asenapine), which are all baseline resolved. The problematic urinary interferences in alcohol metabolites analysis were resolved without the use of buffer or additional mobile phases. The ESI- panel with barbiturates achieved partial resolution of amobarbital and pentobarbital, which may eliminate the need for confirmatory testing.<\/p>\n\n\n\n<p>This work demonstrates that one LC-MS\/MS setup is possible for the analysis of multiple panels, which simplifies testing procedures, saves time, and ultimately reduces costs.<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-consolidating-LC-MSMS-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of poster-consolidating-LC-MSMS-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse.\"><\/object><a id=\"wp-block-file--media-6bf1bfb8-c329-4a1b-b88e-5a61bde4847b\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-consolidating-LC-MSMS-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse.pdf\">poster-consolidating-LC-MSMS-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-consolidating-LC-MSMS-method-conditions-for-the-analysis-of-alcohol-metabolites-barbiturates-and-drugs-of-abuse.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-6bf1bfb8-c329-4a1b-b88e-5a61bde4847b\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>A panel of 136 ESI+\/ESI- therapeutic drugs; drugs of abuse and their metabolites; and biomarkers of alcohol consumption were analyzed using a Force Biphenyl analytical column equipped with a guard column and an online filter. Mobile phase A consisted of 0.1% formic acid in water, mobile phase B consisted of 0.1% formic acid in methanol,&#8230;<\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[813],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[],"ppma_author":[436,579,702],"class_list":["post-84076","post","type-post","status-publish","format-standard","hentry","category-posters-presentations-it"],"acf":[],"taxonomy_info":{"category":[{"value":813,"label":"Posters &amp; Presentations"}]},"featured_image_src_large":false,"author_info":{"display_name":"Emanuele Ceccon","author_link":"https:\/\/discover.restek.com\/it\/author\/emanuele-ceccon\/"},"comment_info":0,"category_info":[{"term_id":813,"name":"Posters &amp; Presentations","slug":"posters-presentations-it","term_group":0,"term_taxonomy_id":813,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":813,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations-it","category_parent":0}],"tag_info":false,"authors":[{"term_id":436,"user_id":16,"is_guest":0,"slug":"emanuele-ceccon","display_name":"Emanuele Ceccon","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-square-ceccon_emanuele.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-square-ceccon_emanuele.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":702,"user_id":0,"is_guest":1,"slug":"justin-steimling","display_name":"Justin Steimling","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/84076","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/comments?post=84076"}],"version-history":[{"count":1,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/84076\/revisions"}],"predecessor-version":[{"id":84078,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/posts\/84076\/revisions\/84078"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/media?parent=84076"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/categories?post=84076"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/tags?post=84076"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/industries-application?post=84076"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/post-badge?post=84076"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-type?post=84076"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/product-library?post=84076"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/resource-technique?post=84076"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/hf_cat_post?post=84076"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/it\/wp-json\/wp\/v2\/ppma_author?post=84076"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}