Numerous articles have been published on faster methods for gas chromatography (GC), yet uncertainty remains on how best to speed up separations. Here, Dr. Hans-Gerd Janssen clarifies the confusion surrounding fast GC and recommends a strategic approach based on 3 steps: 1) minimizing resolution to a value just sufficient, 2) maximizing the selectivity of the chromatographic system, and 3) implementing a method that reduces analysis time while holding resolution constant.
Hydrogen offers a number of benefits over helium as a carrier gas for gas chromatography. With basic safety measures in place, labs can safely use hydrogen for faster chromatography. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
A detailed discussion of how syringe needle length impacts quantitative analysis. Includes optimal needle lengths for different scenarios. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
Discussion of the origins of the retention gap, a length of uncoated tubing used to focus large volume injections and prevent band broadening. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
The rule of using empty liners with splitless injection was developed in the days of manual injections. Does it still hold true? Sample evaporation is explored. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
Proper sample evaporation in the injection port cannot be assumed. Principles governing evaporation are discussed. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
Even validated methods run on certified equipment are unlikely to be foolproof. Real quality assurance needs to be based on direct verification using internal standards and control samples. By Dr. Konrad Grob, Kantonales Laboratory, Zurich
Scaling down column size is simple to do and can help reduce solvent use. Here is an easy-to-follow example that teaches you how to reduce column internal diameter.
Learn what de-wetting is, and how to avoid it, by using an Aqueous C18 column, instead of a conventional C18, when analyzing polar compounds by HPLC.
Protein precipitation is frequently used to minimize matrix impact when analyzing biological samples. However, the effects of highly organic protein precipitation sample extracts on LC peak shape can negatively impact accurate quantification. Dilution or further sample preparation steps are often used to minimize these effects; however, here we show that direct injection of sample extracts is a viable option that can be used to prevent peak distortion, while avoiding the time and variability associated with additional sample preparation.
