In previous blog posts for this series on QuEChERS, we discussed QuECHERS dSPE Selection, Modifying QuECHERS..-Dry Samples and Modifying QuEChERS..-High Fat Samples Once again, I suggest to use the following from the official QuEChERS website (QuEChERS.com), maintained by CVUA Stuttgart,…
Glyphosate is a broad-spectrum herbicide widely used throughout the world. The International Agency for Research on Cancer classified glyphosate as a probable human carcinogen. The safety residual level of glyphosate in food is regulated in the USA and Europe as well as other regions. Direct analysis of underivatized glyphosate, however, can be challenging due to its minimum retention on a reversed-phase or hydrophilic interaction chromatography (HILIC) column and severe adsorption onto the stainless-steel flow path. On the other hand, glyphosate and other polar anionic contaminants tend to strongly retain on an anion-exchange column and require high salt mobile phase to elute, which is not friendly to mass spectrometric analysis.
I can tell you that determining transitions (SRMs) for GC-MS/MS can be quite challenging with so many precursor ions to evaluate. I spent a considerable amount of time optimizing transitions for our GC Multiresidue Pesticide Kit which has 203 compounds….
