31 Jul 2025
Injection volumes, as well as optimal flow rates, are limited by the size of the column. Ideally, if your sample is in the same solvent as your mobile phase, approximate volumes should range as follows:
| Column ID | Volume (µL) |
| 2.1 mm (30 -100 mm length) | 1-3 |
| 3.0-3.2 mm (50-150 mm length) | 2-12 |
| 4.6 mm (50-250 mm length) | 8-40 |
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