If you have a late-eluting peak that elutes very close to the end of your analysis under your original run conditions, it is possible that under the translated run conditions the peak will not elute without adding an isocratic hold. Alternatively, if you are able to reduce your instrument’s dwell volume that can also affect compound retention time, allowing the late-eluting peak to elute within the translated method conditions.
The translated Compound Retention Time is later than the end time of the method program. Why is this the case?
30 Jul 2025
Author
GNOT5140
