The development and optimization of Liquid Chromatography (LC) separations can be time-consuming and costly, often requiring a number of steps including literature research, column selection, method scouting, method development, and method optimization. In an effort to eliminate these steps, an instrument-free, software modeling tool was developed that gives users the ability to select compounds from a database and instantly model a separation on different column phases.
Optimization of the chromatogram model can be performed while maintaining critical pair separations by adjusting parameters, such as instrument/system effects (e.g., dwell volume and extra column volume), mobile phase preferences, number of gradient steps, and more. The first version contains an initial library of approximately 250 Drugs of Abuse (DoA) with plans to expand by adding additional compounds, more libraries, and even more utility.
The modeler delivers a fast, no-cost starting point for LC method developers, novice, and expert, who either lack the expertise, or the time, to develop or optimize separations quickly and accurately. This novel, virtual method development software can improve turnaround time, increase throughput to existing methods, and offer an on-demand consultative user experience.
In this seminar, you will learn how to use Restek’s free Pro EZLC Chromatogram Modeler in order to develop and optimize LC-MS/MS methods before even setting foot in the lab.
