{"id":3074,"date":"2025-07-30T21:13:12","date_gmt":"2025-07-30T21:13:12","guid":{"rendered":"https:\/\/discover.restek.com\/?p=3074"},"modified":"2025-12-09T00:42:53","modified_gmt":"2025-12-09T00:42:53","slug":"what-types-of-samples-and-analytes-can-be-processed-with-sle","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/faq-fr\/gnot5192\/what-types-of-samples-and-analytes-can-be-processed-with-sle","title":{"rendered":"What types of samples and analytes can be processed with SLE?"},"content":{"rendered":"\n<p>For many compounds, excellent analytical results can be obtained with a quick-and-easy SLE cleanup. As shown in this comparison, accurate and precise results\u2014with performance matching a leading supplier\u2014were obtained for acidic, basic, and neutral analytes following a simple Resprep SLE cleanup of plasma samples.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3399-07-1024x402.jpg\" alt=\"chart showing furosemide, ketoprofen, amphetamine, methadone, betamethasone, and prednisone recovery using SLE\" class=\"wp-image-2927\" title=\"-\"><\/figure>\n\n\n\n<p><em>Experimental detail: Gender-pooled and K2EDTA-treated human plasma samples were fortified at concentrations ranging from 0.0125 \u00b5g\/mL to 2.18 \u00b5g\/mL. Acidic compound aliquots were diluted in 2% formic acid (aqueous) while basic compound aliquots were diluted in 5% ammonium hydroxide (aqueous). A 200 \u00b5L diluted sample (1:1 dilution with pretreatment solutions) was loaded onto a 200 mg Resprep SLE well plate. After five minutes, the samples were eluted with 1 ml of 50:50 dichloromethane:ethyl acetate. Then, 100 \u00b5L 50 mM HCl was added to the basic pretreated wells, and, all wells were blown down to dryness and reconstituted in 1:1 methanol:water. Recovery values were determined using matrix-matched calibration. N\u226536 wells for each treatment; 4.7\u201314.4 %RSD across all compounds.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>For many compounds, excellent analytical results can be obtained with a quick-and-easy SLE cleanup. As shown in this comparison, accurate and precise results\u2014with performance matching a leading supplier\u2014were obtained for acidic, basic, and neutral analytes following a simple Resprep SLE cleanup of plasma samples. Experimental detail: Gender-pooled and K2EDTA-treated human plasma samples were fortified at&#8230;<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[801],"tags":[2688,2716],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[414],"class_list":["post-3074","post","type-post","status-publish","format-standard","hentry","category-faq-fr","tag-faq-sample-preparation","tag-faq-supported-liquid-extraction-sle"],"acf":[],"taxonomy_info":{"category":[{"value":801,"label":"FAQ"}],"post_tag":[{"value":2688,"label":"FAQ: Sample Preparation"},{"value":2716,"label":"FAQ: Supported Liquid Extraction (SLE)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":801,"name":"FAQ","slug":"faq-fr","term_group":0,"term_taxonomy_id":801,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":801,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq-fr","category_parent":0}],"tag_info":[{"term_id":2688,"name":"FAQ: Sample Preparation","slug":"faq-sample-preparation","term_group":0,"term_taxonomy_id":2688,"taxonomy":"post_tag","description":"Browse frequently asked questions for sample preparation products.","parent":0,"count":52,"filter":"raw"},{"term_id":2716,"name":"FAQ: Supported Liquid Extraction (SLE)","slug":"faq-supported-liquid-extraction-sle","term_group":0,"term_taxonomy_id":2716,"taxonomy":"post_tag","description":"Browse frequently asked questions for SLE products.","parent":0,"count":3,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3074","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=3074"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3074\/revisions"}],"predecessor-version":[{"id":71376,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3074\/revisions\/71376"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=3074"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=3074"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=3074"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=3074"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=3074"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=3074"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=3074"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=3074"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=3074"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}