The plot has lines that represent hypothetical retention times for compounds that elute at different points in the gradient profile. They are not representations of your actual chromatography. In addition, the time you enter as the end of the gradient (7 minutes in this example) is the time at which the pumps switch over to the final %B. Extra time is required for that final mobile phase concentration to travel through the system to the detector.<\/p>\n","protected":false},"excerpt":{"rendered":"
The plot has lines that represent hypothetical retention times for compounds that elute at different points in the gradient profile. They are not representations of your actual chromatography. In addition, the time you enter as the end of the gradient (7 minutes in this example) is the time at which the pumps switch over to…<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[800],"tags":[2685],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[414],"class_list":["post-3081","post","type-post","status-publish","format-standard","hentry","category-faq-zh","tag-faq-pro-ezlc-method-translator"],"acf":[],"taxonomy_info":{"category":[{"value":800,"label":"FAQ"}],"post_tag":[{"value":2685,"label":"FAQ: Pro EZLC Method Translator"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":800,"name":"FAQ","slug":"faq-zh","term_group":0,"term_taxonomy_id":800,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":800,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq-zh","category_parent":0}],"tag_info":[{"term_id":2685,"name":"FAQ: Pro EZLC Method Translator","slug":"faq-pro-ezlc-method-translator","term_group":0,"term_taxonomy_id":2685,"taxonomy":"post_tag","description":"Browse frequently asked questions about Restek's Pro EZ<\/em>LC Method Translator.","parent":0,"count":12,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3081","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=3081"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3081\/revisions"}],"predecessor-version":[{"id":70977,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3081\/revisions\/70977"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=3081"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=3081"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=3081"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=3081"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=3081"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=3081"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=3081"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=3081"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=3081"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}