{"id":3343,"date":"2025-07-31T14:31:14","date_gmt":"2025-07-31T14:31:14","guid":{"rendered":"https:\/\/discover.restek.com\/?p=3343"},"modified":"2025-12-09T00:07:35","modified_gmt":"2025-12-09T00:07:35","slug":"is-my-raptor-hilic-si-column-ready-to-use-right-out-of-the-box","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/faq\/gnot5084\/is-my-raptor-hilic-si-column-ready-to-use-right-out-of-the-box","title":{"rendered":"Is my Raptor HILIC-Si column ready to use right out of the box?"},"content":{"rendered":"\n<p>Prior to use, your Raptor HILIC-Si column must be properly conditioned with the mobile phase that will be used during analysis. For isocratic HILIC methods, at least 50 column volumes should be used and, for gradient HILIC methods, at least 10 blank injections running the complete time program should be performed. Conditioning is also required when you change the mobile phase composition or the concentration of any additives. Use the same number of column volumes or blank injections as you did when conditioning the column for the first time. Table I below gives the column volumes for Raptor HILIC-Si columns based on their dimensions.<\/p>\n\n\n\n<p><strong>Table I:<\/strong>&nbsp;Column Volumes (mL) Based upon Raptor HILIC-Si Column Dimensions<\/p>\n\n\n<style>.kb-table-container3343_8067df-99{overflow-x:auto;}.kb-table-container .kb-table3343_8067df-99 th{padding-top:var(--global-kb-spacing-xxs, 0.5rem);padding-right:var(--global-kb-spacing-xxs, 0.5rem);padding-bottom:var(--global-kb-spacing-xxs, 0.5rem);padding-left:var(--global-kb-spacing-xxs, 0.5rem);text-align:center;}.kb-table-container .kb-table3343_8067df-99 caption{text-align:center;}.kb-table-container .kb-table3343_8067df-99 td{padding-top:var(--global-kb-spacing-xxs, 0.5rem);padding-right:var(--global-kb-spacing-xxs, 0.5rem);padding-bottom:var(--global-kb-spacing-xxs, 0.5rem);padding-left:var(--global-kb-spacing-xxs, 0.5rem);text-align:left;}<\/style><div class=\"kb-table-container kb-table-container3343_8067df-99 wp-block-kadence-table\"><table class=\"kb-table kb-table3343_8067df-99\">\n<tr class=\"kb-table-row kb-table-row3343_fc69c2-f4\">\n<td class=\"kb-table-data kb-table-data3343_5cc109-db\">\n\n<p><strong>Column ID<\/strong><\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_67dbc7-16\">\n\n<p><strong>30 mm Length<\/strong> <\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_df936e-12\">\n\n<p><strong>50 mm Length<\/strong> <\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_2ee634-66\">\n\n<p><strong>100 mm Length<\/strong><\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_d43e79-3c\">\n\n<p><strong>150 mm Length<\/strong><\/p>\n\n<\/td>\n<\/tr>\n\n<tr class=\"kb-table-row kb-table-row3343_e11c8e-ff\">\n<td class=\"kb-table-data kb-table-data3343_526837-a1\">\n\n<p>2.1 mm<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_2c713b-81\">\n\n<p>0.05<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_038b9c-4e\">\n\n<p>0.1<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_edb711-b7\">\n\n<p>0.2<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_f94cb7-8b\">\n\n<p>0.3<\/p>\n\n<\/td>\n<\/tr>\n\n<tr class=\"kb-table-row kb-table-row3343_04855c-0a\">\n<td class=\"kb-table-data kb-table-data3343_d97523-36\">\n\n<p>3.0 mm<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_700a0a-f2\">\n\n<p>\u2014<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_e894e5-b2\">\n\n<p>0.2<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_e08c30-fa\">\n\n<p>0.4<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_aba27c-69\">\n\n<p>0.5<\/p>\n\n<\/td>\n<\/tr>\n\n<tr class=\"kb-table-row kb-table-row3343_5198bf-1f\">\n<td class=\"kb-table-data kb-table-data3343_dc2afd-a9\">\n\n<p>4.6 mm<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_a7a62a-8b\">\n\n<p>\u2014<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_7ceb5e-8f\">\n\n<p>0.4<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_7e8cac-43\">\n\n<p>0.8<\/p>\n\n<\/td>\n\n<td class=\"kb-table-data kb-table-data3343_c1a843-5a\">\n\n<p>1.2<\/p>\n\n<\/td>\n<\/tr>\n<\/table><\/div>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Prior to use, your Raptor HILIC-Si column must be properly conditioned with the mobile phase that will be used during analysis. For isocratic HILIC methods, at least 50 column volumes should be used and, for gradient HILIC methods, at least 10 blank injections running the complete time program should be performed. Conditioning is also required&#8230;<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[23],"tags":[2702,2682,2699],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[414],"class_list":["post-3343","post","type-post","status-publish","format-standard","hentry","category-faq","tag-faq-hilic-methods","tag-faq-hplc-and-uhplc","tag-faq-raptor-lc-columns"],"acf":[],"taxonomy_info":{"category":[{"value":23,"label":"FAQ"}],"post_tag":[{"value":2702,"label":"FAQ: HILIC Methods"},{"value":2682,"label":"FAQ: HPLC and UHPLC"},{"value":2699,"label":"FAQ: Raptor LC Columns"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":23,"name":"FAQ","slug":"faq","term_group":0,"term_taxonomy_id":23,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":23,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq","category_parent":0}],"tag_info":[{"term_id":2702,"name":"FAQ: HILIC Methods","slug":"faq-hilic-methods","term_group":0,"term_taxonomy_id":2702,"taxonomy":"post_tag","description":"Browse frequently asked questions for HILIC methods.","parent":0,"count":6,"filter":"raw"},{"term_id":2682,"name":"FAQ: HPLC and UHPLC","slug":"faq-hplc-and-uhplc","term_group":0,"term_taxonomy_id":2682,"taxonomy":"post_tag","description":"Browse frequently asked questions for HPLC and UHPLC products.","parent":0,"count":61,"filter":"raw"},{"term_id":2699,"name":"FAQ: Raptor LC Columns","slug":"faq-raptor-lc-columns","term_group":0,"term_taxonomy_id":2699,"taxonomy":"post_tag","description":"Browse frequently asked questions for Restek's Raptor LC columns.","parent":0,"count":13,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3343","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=3343"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3343\/revisions"}],"predecessor-version":[{"id":70930,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/3343\/revisions\/70930"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=3343"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=3343"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=3343"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=3343"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=3343"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=3343"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=3343"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=3343"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=3343"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}