This study examines the stability of working solutions prepared by combining neutral and acidic cannabinoid standards. Stability was assessed for 16 cannabinoids across 30 days for solutions prepared in commonly used diluents and stored under typical lab conditions.<\/p>\n\n\n\n
To ensure accurate reporting, it is essential that the stability of cannabinoid standards be studied. For example, if improperly manufactured or stored, tetrahydrocannabinolic acid (THCA) will convert to its methoxy counterpart, tetrahydrocannabinol (THC), giving invalid results for both analytes. To extend stability and simplify the preparation of working standards, Restek has formulated two certified reference materials (CRMs) comprised of 16 commonly analyzed cannabinoids. These standards are formulated in two high-concentration (1000 \u00b5g\/mL) solutions: a cannabinoids acids 7 mix (cat.# 34144) and a cannabinoids neutrals 9 mix (cat.# 34132). Using these two CRMs instead of 16 single-compound standards saves labs time and money, and minimizes preparation errors. More important, because the acids and neutrals are formulated separately in appropriate diluents instead of as a single solution, long-term stability can be increased by preventing decarboxylation of the acids.<\/p>\n\n\n\n
Stability studies on sealed ampuls of both the cannabinoid acids and neutrals CRMs have been performed by Restek under accelerated conditions for standard stability for up to four weeks. However, to assess the stability of working standards, the current study was conducted by combining the cannabinoid CRMs in commonly used diluents and storing the mixed solutions under typical lab conditions.<\/p>\n\n\n\n
The components of each cannabinoid CRM are given below. To measure the stability of a combined solution of Restek\u2019s cannabinoids acids 7 standard (cat.# 34144) and cannabinoids neutrals 9 standard (cat.# 34132), duration, temperature, and diluent parameters (Figure 1) were selected to represent use in a typical production lab. \u00a0Peak response (area) data was then collected and compared. Data was normalized to Day 0 results, and the acceptance criterion was defined as \u00b15% of the original response on Day 0.<\/p>\n\n\n\n
Cannabinoids Acids 7 Standard (cat.# 34144)<\/strong>
1000 \u00b5g\/mL, acetonitrile with 1% DIPEA and 0.05% ascorbic acid