{"id":42452,"date":"2018-01-03T05:00:00","date_gmt":"2018-01-03T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/gc-troubleshooting-high-gc-column-bleed\/"},"modified":"2026-01-28T15:53:17","modified_gmt":"2026-01-28T15:53:17","slug":"gc-troubleshooting-high-gc-column-bleed","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/videos-zh\/gnav3597\/gc-troubleshooting-high-gc-column-bleed","title":{"rendered":"GC Troubleshooting\u2014High GC Column Bleed"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"GC Troubleshooting\u2014High GC Column Bleed\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/767MCWu86pQ?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description&nbsp;<\/h2>\n\n\n\n<p>High bleed is one of the most common issues&nbsp;you\u2019ll encounter&nbsp;when doing gas chromatography. Excessive bleed is often caused by something damaging the stationary phase of the column, things like oxygen from a leak or some aggressive component in the sample.&nbsp;Checking for leaks, installing&nbsp;inline oxygen filters on carrier gas, and taking care to ensure the sample isn\u2019t damaging the column are key steps to eliminating high-bleed problems.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/chromablography\/column-bleed-septa-bleed-same-old-thing\" target=\"_blank\" rel=\"noopener\">Column Bleed &amp; Septa Bleed\u2014Same Old Thing!<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/ultra-low-bleed-columns-improve-trace-level-analysis\" target=\"_blank\" rel=\"noopener\">Ultra\u2013Low Bleed Columns Improve Trace\u2013Level Analysis<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/articles\/rxi-gc-columns\" target=\"_blank\" rel=\"noopener\">Restek&#8217;s Inert and Low\u2013Bleed Rxi GC Columns<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Hi, folks! Welcome to another Restek Tip. We&#8217;re going to talk about high bleed. In gas chromatography, what is bleed, anyway?<\/p>\n\n\n\n<p>Bleed is the natural degradation process of our stationary phase. It&#8217;s going to break down a little bit over time, and those components of the stationary phase are going to pass through the column and contribute to some signal. High bleed, though, is when that moves from normal behavior into a problem. The&nbsp;signal&#8217;s&nbsp;really high, it starts occurring at lower temperatures, unusual circumstances. What can cause that to happen? Something is degrading your stationary phase. Most likely, it&#8217;s going to be some oxygen. Oxygen is a column killer. It can come into our system from leaks or contaminated carrier gas. Alternatively, we also can damage our stationary phase with aggressive chemicals. Maybe we&#8217;re injecting some&nbsp;derivatization&nbsp;reagents, or some strong acids, or bases. They also can chew up a column. In that case, likely what we will want to do, take some steps to make our injection a little&nbsp;more mild, so that what we&#8217;re putting on our column isn&#8217;t as aggressive. If we get back to the oxygen problem, though, that&#8217;s something that we want to always keep in our minds. We always want to be leak-checking our GC systems. We always want to be making sure that we are scrubbing oxygen from our carrier gas. Having some type of inline filter to remove that is really important.<\/p>\n\n\n\n<p>Those are the tools and techniques for managing bleed when you&#8217;re doing your GC work. Thanks for joining us for this Restek Tip.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>How do you bandage high bleed in gas chromatography? In this Restek Tip learn how to fix bleed and take care of your GC columns.<\/p>\n","protected":false},"author":11,"featured_media":41070,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[775],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[569],"product-library":[2408,2420],"resource-technique":[2307],"ppma_author":[414],"class_list":["post-42452","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-zh","resource-type-troubleshooting","product-library-gas-chromatography-products-zh","product-library-gc-columns-zh","resource-technique-gas-chromatography-gc-zh"],"acf":[],"taxonomy_info":{"category":[{"value":775,"label":"\u89c6\u9891"}],"resource-type":[{"value":569,"label":"Troubleshooting"}],"product-library":[{"value":2408,"label":"\u6c14\u76f8\u8272\u8c31\u76f8\u5173\u4ea7\u54c1"},{"value":2420,"label":"GC\u8272\u8c31\u67f1"}],"resource-technique":[{"value":2307,"label":"\u6c14\u76f8\u8272\u8c31\uff08GC\uff09"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3597-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":775,"name":"\u89c6\u9891","slug":"videos-zh","term_group":0,"term_taxonomy_id":775,"taxonomy":"category","description":"","parent":0,"count":134,"filter":"raw","cat_ID":775,"category_count":134,"category_description":"","cat_name":"\u89c6\u9891","category_nicename":"videos-zh","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42452","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=42452"}],"version-history":[{"count":5,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42452\/revisions"}],"predecessor-version":[{"id":85312,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42452\/revisions\/85312"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/41070"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=42452"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=42452"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=42452"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=42452"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=42452"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=42452"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=42452"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=42452"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=42452"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}