{"id":42479,"date":"2019-05-21T04:00:00","date_gmt":"2019-05-21T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-column-cleaning-and-regeneration\/"},"modified":"2026-01-28T17:21:39","modified_gmt":"2026-01-28T17:21:39","slug":"lc-column-cleaning-and-regeneration","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/videos-ja\/gnav3616\/lc-column-cleaning-and-regeneration","title":{"rendered":"LC Column Cleaning and Regeneration"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Column Cleaning and Regeneration\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/F4cdVXmjRw8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>Over time, our liquid chromatography columns will accumulate some contamination, especially if we regularly analyze dirty samples. There are some steps that we can take to keep our columns clean. Further, we can do some limited column regeneration as well, which can often help restore some performance to our columns and extend their lifetime.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/articles\/restek-liquid-chromatography-lc-columns-cleaning-recommendations\" target=\"_blank\" rel=\"noopener\">LC Column Cleaning Recommendations<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/restek-lc-guard-column-selector\" target=\"_blank\" rel=\"noopener\">LC Guard Column Selector<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1119\" target=\"_blank\" rel=\"noopener\">LC Columns<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1277\" target=\"_blank\" rel=\"noopener\">Guard Cartridges and Hardware<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1123\" target=\"_blank\" rel=\"noopener\">Mobile Phase Accessories<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Thanks for joining us for the latest Restek tip, where I\u2019ll discuss LC column cleaning and regeneration. Column contamination from adsorption of analyte or matrix impurities can have adverse chromatographic effects\u2014such as split peaks, changes in selectivity, or reduced sensitivity\u2014all of which can ultimately impact quantitation. It is highly recommended to use guard columns or an UltraShield precolumn filter to protect your analytical columns. However, over time, even with this precaution, you may observe decreased column performance, increased backpressure, or other signs of column contamination.<\/p>\n\n\n\n<p>For HPLC or UHPLC column cleaning and regeneration, flushing using a series of stronger solvent combinations is recommended with the flow in the direction shown on the column.&nbsp;For reversed phase columns,&nbsp;flush&nbsp;the column with a minimum of 20 column volumes of each solvent in the order shown in the table starting from 5% methanol in water to highly organic solvents and return to your original mobile phase conditions. For normal phase columns, flush with a minimum of 20 column volumes of each solvent in the order shown in the table starting from&nbsp;isopropyl alcohol to ethanol and return to your original mobile phase conditions. Column volume can be estimated using the formula shown here. It is highly recommended to re-equilibrate your column to your original method conditions by performing a series of injections prior to starting your batch. After column regeneration, it is important to store your LC columns with end plugs securely fastened and with your column manufacturer\u2019s recommended solvent for either short-&nbsp;or long-term storage.<\/p>\n\n\n\n<p>If you enjoy these videos, please like, share,&nbsp;and subscribe. You can post your ideas for a future tech tip in the comments below.&nbsp;Thanks for joining us for the latest Restek Tip.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>In this Restek Tip let\u2019s talk liquid chromatography and specifically, column lifetime. We\u2019ve got some info to share on cleaning and regenerating LC columns.<\/p>\n","protected":false},"author":11,"featured_media":41142,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[774],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[565],"product-library":[2571,2553],"resource-technique":[2343],"ppma_author":[414],"class_list":["post-42479","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-ja","resource-type-maintenance","product-library-lc-columns-ja","product-library-liquid-chromatography-products-ja","resource-technique-liquid-chromatography-ja"],"acf":[],"taxonomy_info":{"category":[{"value":774,"label":"\u52d5\u753b"}],"resource-type":[{"value":565,"label":"Maintenance"}],"product-library":[{"value":2571,"label":"LC \u30ab\u30e9\u30e0"},{"value":2553,"label":"\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc"}],"resource-technique":[{"value":2343,"label":"\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3616-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":774,"name":"\u52d5\u753b","slug":"videos-ja","term_group":0,"term_taxonomy_id":774,"taxonomy":"category","description":"","parent":0,"count":136,"filter":"raw","cat_ID":774,"category_count":136,"category_description":"","cat_name":"\u52d5\u753b","category_nicename":"videos-ja","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42479","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=42479"}],"version-history":[{"count":6,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42479\/revisions"}],"predecessor-version":[{"id":85603,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42479\/revisions\/85603"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/41142"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=42479"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=42479"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=42479"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=42479"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=42479"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=42479"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=42479"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=42479"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=42479"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}