{"id":42493,"date":"2018-01-03T05:00:00","date_gmt":"2018-01-03T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-tubing-fitting-choices\/"},"modified":"2026-01-28T15:55:54","modified_gmt":"2026-01-28T15:55:54","slug":"lc-tubing-fitting-choices","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/videos\/gnav3623\/lc-tubing-fitting-choices","title":{"rendered":"LC Tubing &#038; Fitting Choices"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Tubing &amp; Fitting Choices\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/C2AGarS6wG4?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n<div class=\"row\">\n<div class=\"col-12\">\n<h2 aria-level=\"3\"><span data-contrast=\"none\">Description<\/span><\/h2>\n<p><span data-contrast=\"auto\">Tubing is a fact of life for all HPLC and UHPLC instruments, but making sure you have the correct tubing\u00a0<\/span><span data-contrast=\"auto\">and fittings\u00a0<\/span><span data-contrast=\"auto\">for your instrument and application is important.\u00a0\u00a0<\/span><span data-contrast=\"auto\">For tubing and fittings, matching\u00a0<\/span><span data-contrast=\"auto\">your material type and<\/span>\u00a0<span data-contrast=\"auto\">inner diameter<\/span><span data-contrast=\"auto\">\u00a0(ID)<\/span><span data-contrast=\"auto\">\u00a0to the work\u00a0<\/span><span data-contrast=\"auto\">you\u2019re doing\u00a0<\/span><span data-contrast=\"auto\">will help make sure you have the safest, most efficient analysis possible.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<h3>Additional Resources<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.restek.com\/articles\/frequently-asked-questions-hplc-and-uhplc#sec04-item08\" target=\"_blank\" rel=\"noopener\">How do I know what the internal diameter of my LC tubing is?<\/a><\/li>\n<\/ul>\n<h3>Restek Recommended Tools &amp; Parts<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.restek.com\/c\/1121\" target=\"_blank\" rel=\"noopener\">LC Tubing and Connectors<\/a><\/li>\n<\/ul>\n<div>\n<h3>Connect with Us<\/h3>\n<p><span data-contrast=\"auto\">YouTube:\u00a0<\/span><a href=\"https:\/\/www.youtube.com\/user\/RestekCorp\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"exc\" aria-label=\"https:\/\/www.youtube.com\/user\/RestekCorp - opens in new tab\" data-uw-rm-ext-link=\"\"><span data-contrast=\"none\">https:\/\/www.youtube.com\/user\/RestekCorp<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<br role=\"presentation\" data-uw-rm-sr=\"\" \/><\/span><span data-contrast=\"auto\">LinkedIn:\u00a0<a href=\"https:\/\/www.linkedin.com\/company\/restek-corporation\/\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"exc\" aria-label=\"https:\/\/www.linkedin.com\/company\/restek-corporation\/ - opens in new tab\" data-uw-rm-ext-link=\"\">https:\/\/www.linkedin.com\/company\/restek-corporation\/<\/a><br role=\"presentation\" data-uw-rm-sr=\"\" \/>Facebook:\u00a0<a href=\"https:\/\/www.facebook.com\/RestekCorporation\" target=\"_blank\" rel=\"noopener\" data-uw-rm-brl=\"false\" aria-label=\"https:\/\/www.facebook.com\/RestekCorporation - opens in new tab\" data-uw-rm-ext-link=\"\">https:\/\/www.facebook.com\/RestekCorporation<\/a><\/span><\/p>\n<\/div>\n<h2 aria-level=\"3\"><span data-contrast=\"none\">Transcript<\/span><\/h2>\n<p><span data-contrast=\"auto\">Hi everyone, thanks for joining us for this Restek Tip. Our topic today is tubing and fittings for LC systems.<\/span><\/p>\n<p><span data-contrast=\"auto\">Now, I&#8217;m sure you&#8217;re familiar with both PEEK and stainless steel, so let&#8217;s start out talking about PEEK tubing. This is used for pressures up to about 5,000\u00a0<\/span><span data-contrast=\"auto\">psi<\/span><span data-contrast=\"auto\">, and you may have experienced what happens if you get beyond this limit. The tubing will release the fitting with an audible popping sound. You&#8217;ll have a leak somewhere, and you notice your system pressure drops to zero. So, if you&#8217;re running a sample, and have an unexpected loss in pressure, the first place to check are the connections at your column or at your mixer, just to make sure that none of these fittings have come loose. Now, there&#8217;s two typical diameters of tubing used for LC systems. The first is a\u00a0<\/span><span data-contrast=\"auto\">0<\/span><span data-contrast=\"auto\">.3 millimeter with a blue band, and these are general<\/span><span data-contrast=\"auto\">&#8211;<\/span><span data-contrast=\"auto\">purpose connections for your pump to your mixer, the mixer up to the autosampler, and sometimes the autosampler out to the column. Another diameter most commonly used i<\/span><span data-contrast=\"auto\">s<\/span><span data-contrast=\"auto\">\u00a0a\u00a0<\/span><span data-contrast=\"auto\">0<\/span><span data-contrast=\"auto\">.13 millimeter with the red stripe, and this is used for connections from your column to your detector, and the small ID keeps the peaks in a tight band, so that helps you get a little better signal and a better detection limit. Now, it&#8217;s really important to use PEEK fittings when you&#8217;re using PEEK tubing, because if we use a metal fitting, the temptation is to overtighten, which will crimp that smaller inner diameter, cause some restriction, and could eventually completely block the flow of mobile phase.<\/span><\/p>\n<p><span data-contrast=\"auto\">Now, if you&#8217;re running above 5,000\u00a0<\/span><span data-contrast=\"auto\">psi<\/span><span data-contrast=\"auto\">, or have a UHPLC system, you always want to use stainless steel tubing, and they come in the same dimensions and the same color codes as the PEEK, so it&#8217;s very easy to transfer between systems. Now, you might be tempted to cut your own stainless-steel tubing, but we don&#8217;t recommend it, because it&#8217;s really hard to get a nice, square cut and to remove all the burrs from the cutting process. By having a really nice, clean<\/span><span data-contrast=\"auto\">,<\/span><span data-contrast=\"auto\">\u00a0square cut, you can seat the tubing into the port properly, reduce leaks or places where sample can build up and cause carryover in betwe<\/span><span data-contrast=\"auto\">en runs. As far as fitting<\/span><span data-contrast=\"auto\">s<\/span><span data-contrast=\"auto\">\u00a0go for stainless steel tubing, we recommend the EXP type. This has a metal nut and a ferrule with a polymer cone that fits into the port, and a metal portion that connects to the nut.<\/span><\/p>\n<p><span data-contrast=\"auto\">So, no matter what type of system you have, we&#8217;ve got the tubing and the fittings that&#8217;ll keep you up and running. Thanks for joining us, and stay tuned for more tips from your friends at Restek.<\/span><\/p>\n<\/div>\n<\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>It\u2019s LC tubing time again! If it is not cut correctly, you can have a host of problems. Learn some tips of the tubing trade in this Restek Tip.<\/p>\n","protected":false},"author":11,"featured_media":41184,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[8],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2504,2481],"resource-technique":[2326],"ppma_author":[414],"class_list":["post-42493","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos","product-library-lc-schlauche-fittings","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie"],"acf":[],"taxonomy_info":{"category":[{"value":8,"label":"Videos"}],"product-library":[{"value":2504,"label":"LC Schl\u00e4uche &amp; Fittings"},{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3623-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":8,"name":"Videos","slug":"videos","term_group":0,"term_taxonomy_id":8,"taxonomy":"category","description":"","parent":0,"count":136,"filter":"raw","cat_ID":8,"category_count":136,"category_description":"","cat_name":"Videos","category_nicename":"videos","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42493","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=42493"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42493\/revisions"}],"predecessor-version":[{"id":85373,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42493\/revisions\/85373"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/41184"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=42493"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=42493"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=42493"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=42493"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=42493"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=42493"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=42493"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=42493"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=42493"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}