{"id":42556,"date":"2022-12-02T04:00:00","date_gmt":"2022-12-02T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-leak-checking\/"},"modified":"2026-01-27T23:29:08","modified_gmt":"2026-01-27T23:29:08","slug":"lc-leak-checking","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/videos-zh\/gnav3683\/lc-leak-checking","title":{"rendered":"LC Leak Checking"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Leak Checking\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/eJZDdd6SkE8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>Leaks are one of the most common problems users encounter when performing liquid chromatography. Even the smallest leak in your LC system can cause a loss of sample, a drop in efficiency, and break your entire analysis workflow.<\/p>\n\n\n\n<p>In this Restek Tip, we investigate the signs of a possible leak and outline the troubleshooting steps you should take if a leak is discovered.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/discover.restek.com\/articles\/GNAR3485\/effective-lc-troubleshooting-symptom-based-strategies-and-solutions\/\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/articles\/GNAR3485\/effective-lc-troubleshooting-symptom-based-strategies-and-solutions\/\">Effective LC Troubleshooting<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/discover.restek.com\/articles\/GNAR3214\/routine-lc-maintenance\/\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/articles\/GNAR3214\/routine-lc-maintenance\/\">Routine LC Maintenance<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts&nbsp;<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/search?Ntt=EXP&amp;Ns=bestMatch\" target=\"_blank\" rel=\"noopener\">EXP Reusable Fittings<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1119\" target=\"_blank\" rel=\"noopener\">Restek\u2019s HPLC &amp; UHPLC Columns<\/a><\/li>\n<\/ul>\n\n\n\n<p><strong>Restek&#8217;s LC Accessories<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1122\" target=\"_blank\" rel=\"noopener\">LC-MS Supplies<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1118\" target=\"_blank\" rel=\"noopener\">LC Autosampler Supplies<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1123\" target=\"_blank\" rel=\"noopener\">Mobile Phase Management<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1120\" target=\"_blank\" rel=\"noopener\">LC Detector Supplies<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1121\" target=\"_blank\" rel=\"noopener\">LC Tubing &amp; Fittings<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1124\" target=\"_blank\" rel=\"noopener\">LC Pump Supplies<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1125\" target=\"_blank\" rel=\"noopener\">Tools &amp; Organization<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Leaks are one of the most common problems that users encounter when doing liquid chromatography. Even the smallest leak in your system can cause a loss of sample, a drop in efficiency, and break your entire analysis workflow.<\/p>\n\n\n\n<p>In today\u2019s Restek Tip, we\u2019re going to explore the signs of a possible leak and what you should do if a leak is discovered.<\/p>\n\n\n\n<p>Modern LC instruments have sensitive leak detectors in every component, so if the system detects a leak, the pumps will automatically shut off to avoid a solvent spill. Unfortunately, this also means that any current run or batch will stop too.<\/p>\n\n\n\n<p>But even if the instrument doesn\u2019t detect a leak, one could still be present if you notice a loss of retention, signal response\u2014and most importantly\u2014pressure! So, what should you do?<\/p>\n\n\n\n<p>If the instrument displays a leak error code, start by visually inspecting every component of your LC.<\/p>\n\n\n\n<p>If you see where the leak is coming from, tighten the tubing with the appropriate wrench.<\/p>\n\n\n\n<p>If the leak is occurring at the inlet of the LC column, make sure the end-fittings between the mobile phase tubing and the column are tightened and secured; taking care not to overtighten and damage the fitting.<\/p>\n\n\n\n<p>If a leak is still present, remove the fitting and inspect it for any signs of damage. You can also consider using high-pressure fittings, which are more durable.<\/p>\n\n\n\n<p>What if the source of the leak is not easy to find?<\/p>\n\n\n\n<p>Start by following the entire mobile phase flow path with a lab wipe. Watch the wipe for any signs that it\u2019s absorbing liquid, and then troubleshoot the affected component according to the manufacturer\u2019s recommendations.<\/p>\n\n\n\n<p>Modern instruments will not start again until the leak detector is completely dry, so use a clean lab wipe to dry the area first, then wait a couple of minutes before restarting the system.<\/p>\n\n\n\n<p>Solvent leaks can happen at any time, so we recommend making leak checking part of your routine maintenance by looking for any residues or moisture.<\/p>\n\n\n\n<p>Also check for worn or damaged parts, so you can replace them before a leak occurs.<\/p>\n\n\n\n<p>If you have any questions about leak checking your instrument, contact us at restek.com. Thank you for joining us for this Restek Tip!<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Leaks are one of the most common problems users encounter when performing liquid chromatography. But even the smallest leak can cause a loss of sample, a drop in efficiency, and break entire analysis workflows. In this video, learn how to best troubleshoot LC leaks.<\/p>\n","protected":false},"author":11,"featured_media":41370,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[775],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2504,2481],"resource-technique":[2326],"ppma_author":[414],"class_list":["post-42556","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-zh","product-library-lc-schlauche-fittings","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie"],"acf":[],"taxonomy_info":{"category":[{"value":775,"label":"\u89c6\u9891"}],"product-library":[{"value":2504,"label":"LC Schl\u00e4uche &amp; Fittings"},{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3683-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":775,"name":"\u89c6\u9891","slug":"videos-zh","term_group":0,"term_taxonomy_id":775,"taxonomy":"category","description":"","parent":0,"count":136,"filter":"raw","cat_ID":775,"category_count":136,"category_description":"","cat_name":"\u89c6\u9891","category_nicename":"videos-zh","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42556","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=42556"}],"version-history":[{"count":5,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42556\/revisions"}],"predecessor-version":[{"id":85014,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/42556\/revisions\/85014"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/41370"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=42556"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=42556"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=42556"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=42556"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=42556"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=42556"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=42556"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=42556"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=42556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}