{"id":43362,"date":"2025-04-08T00:00:00","date_gmt":"2025-04-08T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-ms-ms-analysis-of-thc-isomers-and-metabolites-in-whole-blood-and-urine-japan\/"},"modified":"2025-11-25T20:52:52","modified_gmt":"2025-11-25T20:52:52","slug":"lc-ms-ms-analysis-of-thc-isomers-and-metabolites-in-whole-blood-and-urine-japan","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/application-notes-ja\/cfan4420-ja\/lc-ms-ms-analysis-of-thc-isomers-and-metabolites-in-whole-blood-and-urine-japan","title":{"rendered":"\u5168\u8840\u30fb\u5c3f\u4e2dTHC\u7570\u6027\u4f53\u304a\u3088\u3073\u4ee3\u8b1d\u7269\u306e\u9ad8\u611f\u5ea6LC-MS\/MS\u30e1\u30bd\u30c3\u30c9"},"content":{"rendered":"\n

\u6982\u8981<\/h2>\n\n\n\n

THC\u306e\u4ee3\u8b1d\u7269\u691c\u67fb\u306e\u91cd\u8981\u6027\u306f\u3001\u6cd5\u533b\u5b66\u30fb\u533b\u7642\u73fe\u5834\u306b\u304a\u3044\u3066\u8fd1\u5e74\u307e\u3059\u307e\u3059\u9ad8\u307e\u3063\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u03948-THC\u3001\u03949-THC\u306a\u3069\u306e\u7570\u6027\u4f53\u304a\u3088\u3073\u305d\u308c\u3089\u306e\u4ee3\u8b1d\u7269\u306f\u5316\u5b66\u69cb\u9020\u304c\u975e\u5e38\u306b\u985e\u4f3c\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u5f93\u6765\u306e\u5206\u6790\u6cd5\u3067\u306f\u6b63\u78ba\u306b\u8b58\u5225\u3059\u308b\u3053\u3068\u304c\u56f0\u96e3\u3067\u3059\u3002\u305d\u3053\u3067\u672c\u7814\u7a76\u3067\u306f\u3001\u3053\u306e\u8ab2\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u306b\u3001\u5168\u8840\u304a\u3088\u3073\u5c3f\u30b5\u30f3\u30d7\u30eb\u3092\u5bfe\u8c61\u3068\u3057\u305f\u65b0\u305f\u306aLC-MS\/MS\u5206\u6790\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u306f\u3058\u3081\u306b<\/h2>\n\n\n\n

\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb(\u03949-THC)\u306e\u6442\u53d6\u306b\u95a2\u3059\u308b\u8840\u6db2\u304a\u3088\u3073\u5c3f\u306e\u691c\u67fb\u306f\u3001\u85ac\u7269\u4f7f\u7528\u306e\u5224\u5b9a\u3084\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u306e\u624b\u6cd5\u3068\u3057\u3066\u9577\u5e74\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u4f53\u5185\u306b\u53d6\u308a\u8fbc\u307e\u308c\u305f\u03949-THC\u306f\u300111-\u30ce\u30eb-9-\u30ab\u30eb\u30dc\u30ad\u30b7-\u03949-THC\uff08\u03949-THC-COOH\uff09\u3078\u3068\u4ee3\u8b1d\u3055\u308c\u3001\u3053\u308c\u3089\u304c\u8840\u6db2\u3084\u5c3f\u304b\u3089\u691c\u51fa\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001\u03949-THC\u3068\u305d\u306e\u4ee3\u8b1d\u7269\u3067\u3042\u308b\u03949-THC-COOH\u306e\u4e8c\u3064\u3060\u3051\u304c\u3001THC\u6442\u53d6\u5224\u5b9a\u306e\u4e3b\u8981\u6307\u6a19\u3068\u3055\u308c\u3066\u304d\u307e\u3057\u305f [1]\u3002\u3057\u304b\u3057\u3001\u3088\u308a\u591a\u69d8\u306a\u03949-THC\u7570\u6027\u4f53\u304c\u5e02\u5834\u3067\u6d41\u901a\u3059\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u3053\u3068\u3067\u3001\u691c\u67fb\u306f\u6025\u6fc0\u306b\u8907\u96d1\u5316\u3057\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u3001\u30d8\u30f3\u30d7\uff08\u9ebb\uff09\u7531\u6765\u88fd\u54c1\u3092\u5408\u6cd5\u5316\u3057\u305f2018\u5e74\u306e\u8fb2\u696d\u6cd5\u6848\u306e\u6210\u7acb\u4ee5\u964d\u3001\u305d\u306e\u898f\u5236\u306e\u629c\u3051\u7a74\u3092\u5229\u7528\u3057\u305f\u65b0\u305f\u306a\u7570\u6027\u4f53\u306e\u6d41\u901a\u304c\u554f\u984c\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e00\u3064\u3067\u3042\u308b\u03948-THC\u306f\u3001\u4f53\u5185\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u306811-\u30ce\u30eb-9-\u30ab\u30eb\u30dc\u30ad\u30b7-\u03948-THC\uff08\u03948-THC-COOH\uff09\u3068\u3044\u3046\u4ee3\u8b1d\u7269\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u03948-THC-COOH\u306f\u3001\u5f93\u6765\u306e\u4e3b\u8981\u6307\u6a19\u3067\u3042\u308b\u03949-THC-COOH\u3068\u5316\u5b66\u69cb\u9020\u304c\u975e\u5e38\u306b\u985e\u4f3c\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u3053\u308c\u30892\u3064\u306e\u4ee3\u8b1d\u7269\u5206\u96e2\u304c\u4e0d\u5341\u5206\u306a\u5834\u5408\u3001\u7279\u306b\u4e00\u65b9\u306e\u7570\u6027\u4f53\u304c\u9ad8\u6fc3\u5ea6\u3067\u5b58\u5728\u3059\u308b\u72b6\u6cc1\u4e0b\u3067\u306f\u3001\u691c\u67fb\u7d50\u679c\u304c\u8aa4\u3063\u305f\u89e3\u91c8\u306b\u3064\u306a\u304c\u308b\u30ea\u30b9\u30af\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u307e\u305f\u3001\u81e8\u5e8a\u691c\u67fb\u30e9\u30dc\u3084\u6bd2\u7269\u691c\u67fb\u30e9\u30dc\u306b\u3068\u3063\u3066\u3001\u4e8b\u4ef6\u767a\u751f\u5f8c\u306b\u63a1\u53d6\u3057\u305f\u8840\u6db2\u691c\u4f53\u3092\u7528\u3044\u3066\u3001\u5bfe\u8c61\u8005\u304c\u5927\u9ebb\u306e\u5f71\u97ff\u4e0b\u306b\u3042\u3063\u305f\u304b\u3069\u3046\u304b\u3092\u5224\u5b9a\u3067\u304d\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u306e\u3088\u3046\u306a\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u306f\u3001\u4ed6\u306e\u4e00\u822c\u7684\u306a\u4e8b\u4ef6\u5f53\u6642\u306e\u691c\u67fb\u3068\u6bd4\u3079\u3066\u5bb9\u6613\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3068\u3044\u3046\u306e\u3082\u3001\u03949-THC\u306f\u4f53\u5185\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3068\u6025\u901f\u306b\u8840\u4e2d\u6fc3\u5ea6\u304c\u4f4e\u4e0b\u3057\u307e\u3059\u304c\u3001\u305d\u306e\u4ee3\u8b1d\u904e\u7a0b\u306f\u975e\u7dda\u5f62\u3067\u500b\u4eba\u5dee\u3082\u5927\u304d\u3044\u305f\u3081\u3001\u6442\u53d6\u6642\u671f\u3084\u66dd\u9732\u6642\u9593\u3092\u6b63\u78ba\u306b\u63a8\u5b9a\u3059\u308b\u3053\u3068\u306f\u56f0\u96e3\u3067\u3059[1]\u3002\u3055\u3089\u306b\u3001\u30d8\u30d3\u30fc\u30e6\u30fc\u30b6\u30fc\u306e\u5834\u5408\u3001\u6700\u7d42\u63a5\u7a2e\u304b\u308933\u65e5\u5f8c\u306bTHC\u304c\u3001\u307e\u305f\u7d0430\u65e5\u5f8c\u306b\u5c3f\u304b\u3089\u03949-THC-COOH\u304c\u691c\u51fa\u3055\u308c\u305f\u4f8b\u3082\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u5316\u5408\u7269\u306f\u3001\u691c\u4f53\u63a1\u53d6\u6642\u70b9\u3067\u5bfe\u8c61\u8005\u304c\u5927\u9ebb\u306e\u5f71\u97ff\u4e0b\u306b\u3042\u308b\u304b\u3069\u3046\u304b\u3092\u5224\u65ad\u3059\u308b\u6307\u6a19\u3068\u3057\u3066\u306f\u9069\u3057\u3066\u3044\u307e\u305b\u3093[1,2]\u3002\u03949-THC\u306f\u4f53\u5185\u306b\u5165\u308b\u3068\u9178\u5316\u3055\u308c\u3001\u77ed\u547d\u306a\u4e2d\u9593\u4ee3\u8b1d\u7269\u3067\u3042\u308b11-\u30d2\u30c9\u30ed\u30ad\u30b7-\u03949-\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\uff0811-OH-\u03949-THC\uff09\u3068\u306a\u308a\u3001\u6700\u7d42\u7684\u306b\u03949-THC-COOH\u306b\u4ee3\u8b1d\u3055\u308c\u307e\u3059\u300211-OH-\u03949-THC\u5358\u72ec\u3067\u306f\u3001\u5927\u9ebb\u306e\u6b63\u78ba\u306a\u6442\u53d6\u6642\u671f\u3084\u66dd\u9732\u6642\u9593\u3092\u6b63\u78ba\u306b\u63a8\u5b9a\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u304c\u3001\u3053\u306e\u4ee3\u8b1d\u7269\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3059\u308b\u3053\u3068\u3067\u3001\u5bfe\u8c61\u8005\u304c\u6700\u8fd1\u5927\u9ebb\u3092\u6442\u53d6\u3057\u305f\u53ef\u80fd\u6027\u3092\u793a\u3059\u6709\u529b\u306a\u624b\u304c\u304b\u308a\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u672c\u7814\u7a76\u3067\u306f\u30012\u3064\u306e\u5206\u6790\u65b9\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u5c3f\u4e2d\u306e\u03948-THC\u3068\u03949-THC\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u4ee3\u8b1d\u7269\uff08\u03949-THC-COOH\/\u03948-THC-COOH\uff09\u306e\u4e21\u65b9\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3059\u308b\u5206\u6790\u65b9\u6cd5\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306f\u30012\u3064\u306e\u7570\u6027\u4f53\u9593\u3067\u5341\u5206\u306a\u5206\u96e2\u304c\u5f97\u3089\u308c\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u7570\u6027\u4f53\u306e\u4e00\u65b9\u304c\u975e\u5e38\u306b\u9ad8\u6fc3\u5ea6\u3067\u5b58\u5728\u3057\u3066\u3082\u3001\u5b9a\u91cf\u30c7\u30fc\u30bf\u306e\u4fe1\u983c\u6027\u3068\u5b89\u5b9a\u6027\u306f\u7dad\u6301\u3055\u308c\u307e\u3059\u3002\u6b21\u306b\u958b\u767a\u3057\u305f\u306e\u306f\u3001\u89aa\u5316\u5408\u7269(\u03948-THC\/\u03949-THC)\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u4ee3\u8b1d\u7269(11-OH-\u03949-THC\/11-OH-\u03948-THC)\u3001\u30ab\u30eb\u30dc\u30ad\u30b7\u4ee3\u8b1d\u7269(\u03949-THC-COOH\/\u03948-THC-COOH)\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3059\u308b\u305f\u3081\u306e\u3001\u5168\u8840\u3092\u5bfe\u8c61\u3068\u3057\u305f\u5206\u6790\u65b9\u6cd5\u3067\u3059\u3002\u5b9f\u9a13\u8a73\u7d30\u3068\u305d\u306e\u7d50\u679c\u3068\u5171\u306b\u3001\u5404\u5206\u6790\u65b9\u6cd5\u3092\u8a73\u8aac\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

\u5b9f\u9a13<\/h2>\n\n\n\n

\u5206\u6790\u65b9\u6cd5<\/h3>\n\n\n\n

1. \u5c3f<\/strong><\/h4>\n\n\n\n

\u5c3f\u4e2d\u306e\u03948-THC-COOH\u304a\u3088\u3073\u03949-THC-COOH\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5206\u6790\u6761\u4ef6\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3067\u3059\u3002<\/p>\n\n\n

Figure 1:<\/strong> \u5c3f\u4e2d\u306e\u03948-THC-COOH\u304a\u3088\u3073\u03949-THC-COOH\u4ee3\u8b1d\u7269\uff08\u6fc3\u5ea650 ng\/mL\uff09\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\uff08\u5206\u6790\u6761\u4ef6\u306f\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u53ef\uff09<\/div>
\n
\"\u03948-THC-COOH<\/div>

LC_CF0828<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Precursor<\/th>Product 1<\/th>Product 2<\/th><\/tr><\/thead>\n
1.<\/td>\u03948-THC-COOH<\/a><\/td>4.44<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
2.<\/td>\u03949-THC-COOH<\/a><\/td>5.12<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

2. \u8840\u6db2<\/strong><\/h4>\n\n\n\n

\u5168\u8840\u4e2d\u306e\u03949-THC\/\u03948-THC\u3001\u03949-THC-COOH\/\u03948-THC-COOH\u300111-OH-\u03949-THC\/11-OH-\u03948-THC\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5206\u6790\u6761\u4ef6\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3067\u3059\u3002<\/p>\n\n\n

Figure 2:<\/strong> \u5168\u8840\u4e2d\u306e\u5316\u5408\u7269\u03948-THC\u3001\u03949-THC\u300111-OH-\u03949-THC\u300111-OH-\u03948-THC\uff08\u6fc3\u5ea650 ng\/mL\uff09\u3068\u03948-THC-COOH\u3001\u03949-THC-COOH\uff08\u6fc3\u5ea6250 ng\/mL\uff09\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\uff08\u5206\u6790\u6761\u4ef6\u306f\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u53ef\uff09<\/div>
\n
\"\u03949-THC;<\/div>

LC_CF0826<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor<\/th>Product 1<\/th>Product 2<\/th><\/tr><\/thead>\n
1.<\/td>11-OH-\u03948-THC<\/a><\/td>4.66<\/td>50<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
2.<\/td>11-OH-\u03949-THC<\/a><\/td>5.00<\/td>50<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
3.<\/td>\u03948-THC-COOH<\/a><\/td>5.04<\/td>250<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
4.<\/td>\u03949-THC-COOH<\/a><\/td>5.85<\/td>250<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
5.<\/td>\u03948-THC<\/a><\/td>10.88<\/td>50<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
6.<\/td>\u03949-THC<\/a><\/td>11.26<\/td>50<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406<\/h3>\n\n\n\n

\u6a19\u6e96\u7269\u8cea\uff08\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff09<\/h4>\n\n\n\n

\u6821\u6b63\u7528\u6a19\u6e96\u6eb6\u6db2\u3001QC\u7528\u6a19\u6e96\u6eb6\u6db2\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u306f\u30e1\u30bf\u30ce\u30fc\u30eb\u3067\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

1. \u5c3f<\/strong><\/h4>\n\n\n\n

\u5c3f\u6821\u6b63\u7528\u30b5\u30f3\u30d7\u30eb\u304a\u3088\u3073QC\u7528\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406<\/h4>\n\n\n\n

\u30d6\u30e9\u30f3\u30af\u306e\u30d2\u30c8\u5c3f\u306b\u3055\u307e\u3056\u307e\u306a\u6fc3\u5ea6\u306e\u6a19\u6e96\u7269\u8cea\u3092\u6dfb\u52a0\u3057\u3001\u691c\u91cf\u7dda\u7bc4\u56f2\u5185\u3067\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\u5404\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-COOH-D9 \u6fc3\u5ea6:1000ng\/mL\uff0950 \u03bcL\u3092\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u6dfb\u52a0\u3057\u305f\u5f8c\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u52a0\u6c34\u5206\u89e3\u5c3fQC\u7528\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406<\/h4>\n\n\n\n

\u30b0\u30eb\u30af\u30ed\u30cb\u30c9\u967d\u6027\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-COOH-\u30b0\u30eb\u30af\u30ed\u30cb\u30c9\uff09\u3092750ng\/mL\uff08\u904a\u96e2\u6642\uff09\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u30d6\u30e9\u30f3\u30af\u5c3f\u306b\u6dfb\u52a0\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-COOH-D9 \u6fc3\u5ea6:1000ng\/mL\uff0950 \u03bcL\u3092\u30b5\u30f3\u30d7\u30eb\u306b\u6dfb\u52a0\u3057\u305f\u5f8c\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u60a3\u8005\u7531\u6765\u5c3f\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406<\/h4>\n\n\n\n

\u60a3\u8005\u304b\u3089\u63a1\u53d6\u3057\u305f\u5c3f\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-COOH-D9 \u6fc3\u5ea6:1000ng\/mL\uff0950 \u03bcL\u3092\u30b5\u30f3\u30d7\u30eb\u306b\u6dfb\u52a0\u3057\u305f\u5f8c\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u5c3f\u30b5\u30f3\u30d7\u30eb\u306e\u30a2\u30eb\u30ab\u30ea\u52a0\u6c34\u5206\u89e3\u624b\u9806<\/h4>\n\n\n\n

THC\u306f\u4f53\u5185\u3067\u4ee3\u8b1d\u3055\u308c\u308b\u904e\u7a0b\u3067\u30b0\u30eb\u30af\u30ed\u30f3\u9178\u62b1\u5408\u4f53\u3068\u3057\u3066\u5c3f\u4e2d\u306b\u6392\u6cc4\u3055\u308c\u308b\u305f\u3081\u3001\u6b63\u78ba\u306a\u5206\u6790\u3092\u884c\u3046\u306b\u306f\u3001\u5c3f\u691c\u4f53\u3092\u62bd\u51fa\u3059\u308b\u524d\u306e\u52a0\u6c34\u5206\u89e3\u51e6\u7406\u304c\u4e0d\u53ef\u6b20\u3067\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u30a2\u30eb\u30ab\u30ea\u52a0\u6c34\u5206\u89e3\u3067\u3053\u306e\u51e6\u7406\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u5c3f\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u306b\u300110N\u6c34\u9178\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\uff08NaOH\uff0940 \u03bcL\u3092\u6dfb\u52a0\u3057\u307e\u3057\u305f\u3002\u30ad\u30e3\u30c3\u30d7\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u305f\u5f8c\u300160\u2103\u306720\u5206\u9593\u4fdd\u6e29\uff08\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\uff09\u3057\u3001\u30b0\u30eb\u30af\u30ed\u30f3\u9178\u62b1\u5408\u4f53\u3092\u904a\u96e2\u578b\u3078\u3068\u5909\u63db\u3001\u4fdd\u6e29\u5b8c\u4e86\u5f8c\u3001\u51b7\u5374\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306b\u6c37\u9162\u917825 \u03bcL\u3092\u6dfb\u52a0\u3057\u3001pH\u3092\u4e2d\u548c\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u52a0\u6c34\u5206\u89e3\u30b5\u30f3\u30d7\u30eb12 mL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u79fb\u3057\u3001\u6db2\u6db2\u62bd\u51fa\uff08LLE\uff09\u3067\u306e\u62bd\u51fa\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u5c3f\u30b5\u30f3\u30d7\u30eb\u306eLLE\u624b\u9806<\/h4>\n\n\n\n

HPLC\u30b0\u30ec\u30fc\u30c9\u306e\u6c34500 \u03bcL\u3092\u5404\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002\u7d9a\u3044\u3066\u5404\u8a66\u9a13\u7ba1\u306b10\uff05\u9162\u9178100 \u03bcL\u3092\u6dfb\u52a0\u3057\u3001\u518d\u5ea6\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u3001\u30d8\u30ad\u30b5\u30f3:\u9162\u9178\u30a8\u30c1\u30eb\u309280:20\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db22.5 mL\u3092\u5404\u8a66\u9a13\u7ba1\u306b\u6dfb\u52a0\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u8a66\u9a13\u7ba1\u306b\u30ad\u30e3\u30c3\u30d7\u3092\u3057\u3066\u3001\u76ee\u8996\u3067\u6df7\u5408\u304c\u78ba\u8a8d\u3067\u304d\u308b\u307e\u3067\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002\u7d9a\u3044\u3066\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u30922800 rpm\u306715\u5206\u9593\u3001\u307e\u305f\u306f2\u3064\u306e\u5c64\u304c\u5b8c\u5168\u306b\u5206\u96e2\u3059\u308b\u307e\u3067\u9060\u5fc3\u5206\u96e2\u3057\u3001\u4e0a\u6e05\u3092\u6e05\u6f54\u306a\u8a66\u9a13\u7ba1\u306b\u79fb\u3057\u305f\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u7a92\u7d20\u4e0b\u3067\u4e7e\u56fa\u3057\u307e\u3057\u305f\u3002\u4e7e\u56fa\u5f8c\u30010.1%\u30ae\u9178\u3092\u542b\u3080\u6c34:\u30e1\u30bf\u30ce\u30fc\u30eb\u309240:60\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db2100 \u00b5L\u3067\u30b5\u30f3\u30d7\u30eb\u3092\u518d\u6eb6\u89e3\u3057\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002\u6700\u5f8c\u306b\u3001\u518d\u6eb6\u89e3\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092LC\u30d0\u30a4\u30a2\u30eb\u30a4\u30f3\u30b5\u30fc\u30c8\u306b\u79fb\u3057\u3001LC-MS\/MS\u306b\u3088\u308b\u5206\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

2. \u8840\u6db2<\/strong><\/h4>\n\n\n\n

\u8840\u6db2\u6821\u6b63\u7528\u30b5\u30f3\u30d7\u30eb\u304a\u3088\u3073QC\u7528\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406<\/h4>\n\n\n\n

\u30d6\u30e9\u30f3\u30af\u306e\u30d2\u30c8\u8840\u6db2\uff08K2\u30fbEDTA\u3092\u542b\u3080\uff09\u306b\u3055\u307e\u3056\u307e\u306a\u6fc3\u5ea6\u306e\u6a19\u6e96\u7269\u8cea\u3092\u6dfb\u52a0\u3057\u3001\u691c\u91cf\u7dda\u7bc4\u56f2\u5185\u3067\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\u5404\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-D3\u300111-OH-\u03949-THC-D3\u3001\u03949-THC-COOH-D3 \u6fc3\u5ea6:1000ng\/mL\uff0950 \u03bcL\u3092\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u6dfb\u52a0\u3057\u305f\u5f8c\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u60a3\u8005\u7531\u6765\u8840\u6db2\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406<\/h4>\n\n\n\n

\u60a3\u8005\u304b\u3089\u63a1\u53d6\u3057\u305f\u8840\u6db2\u30b5\u30f3\u30d7\u30eb500 \u03bcL\u3092\u30ac\u30e9\u30b9\u88fd\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea\uff08\u03949-THC-D3\u300111-OH-\u03949-THC-D3\u3001\u03949-THC-COOH-D3 \u6fc3\u5ea6:1000ng\/mL\uff0950 \u03bcL\u3092\u30b5\u30f3\u30d7\u30eb\u306b\u6dfb\u52a0\u3057\u305f\u5f8c\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u8840\u6db2\u30b5\u30f3\u30d7\u30eb\u306eLLE\u624b\u9806<\/h4>\n\n\n\n

HPLC\u30b0\u30ec\u30fc\u30c9\u306e\u6c34500 \u03bcL\u3092\u5404\u8a66\u9a13\u7ba1\u306b\u52a0\u3048\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u305f\u306e\u3061\u300110\uff05\u9162\u9178100 \u03bcL\u3092\u6dfb\u52a0\u3057\u3001\u518d\u5ea6\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3057\u307e\u3057\u305f\u3002\u30d8\u30ad\u30b5\u30f3:\u9162\u9178\u30a8\u30c1\u30eb\u309280:20\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db22.5 mL\u3092\u5404\u8a66\u9a13\u7ba1\u306b\u6dfb\u52a0\u5f8c\u3001\u8a66\u9a13\u7ba1\u306b\u30ad\u30e3\u30c3\u30d7\u3092\u3057\u3066\u3001\u76ee\u8996\u3067\u6df7\u5408\u304c\u78ba\u8a8d\u3067\u304d\u308b\u307e\u3067\u64b9\u62cc\uff08\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\uff09\u3057\u307e\u3057\u305f\u3002\u6b21\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u30922800rpm\u306715\u5206\u9593\u3001\u307e\u305f\u306f2\u3064\u306e\u5c64\u304c\u5b8c\u5168\u306b\u5206\u96e2\u3059\u308b\u307e\u3067\u9060\u5fc3\u5206\u96e2\u3057\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u4e0a\u6e05\u3092\u6e05\u6f54\u306a\u8a66\u9a13\u7ba1\u306b\u79fb\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u7a92\u7d20\u4e0b\u3067\u4e7e\u56fa\u3057\u30010.1%\u30ae\u9178\u3092\u542b\u3080\u3001\u6c34:\u30e1\u30bf\u30ce\u30fc\u30eb\u309240:60\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db2100\u00b5L\u3067\u30b5\u30f3\u30d7\u30eb\u3092\u518d\u6eb6\u89e3\u3057\u307e\u3057\u305f\u3002\u6700\u5f8c\u306b\u3001\u518d\u6eb6\u89e3\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u64b9\u62cc\uff08\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\uff09\u3057\u3066LC\u30d0\u30a4\u30a2\u30eb\u30a4\u30f3\u30b5\u30fc\u30c8\u306b\u79fb\u3057\u3001LC-MS\/MS\u306b\u3088\u308b\u5206\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n\n\n\n

1. \u5c3f\u3000<\/strong><\/p>\n\n\n\n

\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6027\u80fd<\/h3>\n\n\n\n

Raptor FluoroPhenyl 2.7 \u00b5m, 100 x 3.0 mm\u30ab\u30e9\u30e0\u3092\u7528\u3044\u305f6.5\u5206\u306e\u30a4\u30bd\u30af\u30e9\u30c6\u30a3\u30c3\u30af\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\uff08\u7dcf\u5206\u6790\u6642\u95938.5\u5206\uff09\u3067\u5206\u6790\u3092\u884c\u3063\u305f\u7d50\u679c\u30012\u3064\u306e\u7570\u6027\u4f53\u306f\u826f\u597d\u306b\u5206\u96e2\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306e\u30e1\u30bd\u30c3\u30c9\u3067\u5f97\u3089\u308c\u305f\u5206\u96e2\u5ea6\u306f\u3001\u5c3f\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u6642\u6298\u898b\u3089\u308c\u308b\u7570\u6027\u4f53\u306e\u6fc3\u5ea6\u306b\u6975\u7aef\u306a\u5dee\u304c\u3042\u308b\u5834\u5408\u3067\u3082\u30012\u3064\u306e\u5206\u6790\u6210\u5206\u9593\u306e\u5b9a\u91cf\u7684\u306a\u5e72\u6e09\u3092\u5341\u5206\u306b\u9632\u3050\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\uff08Figure 3<\/strong>\u304a\u3088\u3073Figure 4<\/strong>\u53c2\u7167\uff09\u3002<\/p>\n\n\n

Figure 3:<\/strong> \u6975\u7aef\u306a\u7570\u6027\u4f53\u6fc3\u5ea6\u6bd4\uff08\u03948-THC-COOH\uff08\u6fc3\u5ea61 ng\/mL\uff09: \u03949-THC-COOH\uff08\u6fc3\u5ea61000 ng\/mL\uff09\uff09\u3067\u3082\u4e92\u3044\u306b\u3088\u304f\u5206\u96e2\u3055\u308c\u3066\u3044\u308b\u03948-THC-COOH\u3068\u03949-THC-COOH\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0<\/div>
\n
\"1000<\/div>

LC_CF0831<\/p>

<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n
Figure 4:<\/strong> \u6975\u7aef\u306a\u7570\u6027\u4f53\u6fc3\u5ea6\u6bd4\uff08\u03949-THC-COOH\uff08\u6fc3\u5ea61 ng\/mL\uff09\uff1a\u03948-THC-COOH\uff08\u6fc3\u5ea61000 ng\/mL\uff09\uff09\u3067\u3082\u4e92\u3044\u306b\u3088\u304f\u5206\u96e2\u3055\u308c\u3066\u3044\u308b\u03948-THC-COOH\u3068\u03949-THC-COOH\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0<\/div>
\n
\"10<\/div>

LC_CF0832<\/p>

<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u76f4\u7dda\u6027<\/h3>\n\n\n\n

1\/x \u91cd\u307f\u4ed8\u3051\u7dda\u5f62\u56de\u5e30\u3092\u7528\u3044\u3066\u76f4\u7dda\u6027\u3092\u78ba\u8a8d\u3057\u305f\u3068\u3053\u308d\u3001\u3059\u3079\u3066\u306e\u5206\u6790\u5bfe\u8c61\u6210\u5206\u304c\u30010.99\u4ee5\u4e0a\u306e\u8a31\u5bb9\u53ef\u80fd\u306ar2<\/sup>\u5024\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u512a\u308c\u305f\u76f4\u7dda\u6027\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002\u03948-THC-COOH\u304a\u3088\u3073\u03949-THC-COOH\u306e\u6821\u6b63\u7bc4\u56f2\u306f\u6fc3\u5ea65\uff5e1000 ng\/mL\uff08\u5c3f\u691c\u4f53\u4e2d\u306b\u304a\u3051\u308b\u5178\u578b\u7684\u306a\u6fc3\u5ea6\u5206\u5e03\u3092\u30ab\u30d0\u30fc\uff09\u306b\u8a2d\u5b9a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u7531\u6765\u306e\u5e72\u6e09\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u3001\u30d6\u30e9\u30f3\u30af\u5c3f\u30b5\u30f3\u30d7\u30eb3\u691c\u4f53\u3092\u524d\u51e6\u7406\u3057\u3066\u5206\u6790\u3057\u3001\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u7531\u6765\u3057\u306a\u3044\u30b7\u30b0\u30ca\u30eb\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u7531\u6765\u306e\u5e72\u6e09\u306f\u8a8d\u3081\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u307e\u305f\u3001\u3082\u3063\u3068\u3082\u9ad8\u3044\u6821\u6b63\u6fc3\u5ea6\uff081000 ng\/mL\uff09\u30b5\u30f3\u30d7\u30eb\u306e\u6ce8\u5165\u5f8c\u306b\u30e1\u30bf\u30ce\u30fc\u30eb\u30d6\u30e9\u30f3\u30af\u3092\u6ce8\u5165\u3057\u3001\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3057\u305f\u3068\u3053\u308d\u3001\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306f\u89b3\u5bdf\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

\u518d\u73fe\u6027\u3068\u7cbe\u5ea6<\/h3>\n\n\n\n

\u518d\u73fe\u6027\u3068\u7cbe\u5ea6\u306e\u8a55\u4fa1\u306f\u3001LLOQ\uff08\u5b9a\u91cf\u4e0b\u9650\uff09\u3001Low QC\uff08\u4f4e\u6fc3\u5ea6QC\uff09\u3001Medium QC\uff08\u4e2d\u6fc3\u5ea6QC\uff09\u3001High QC\uff08\u9ad8\u6fc3\u5ea6QC\uff09\u306e\u30014\u3064\u306e\u7570\u306a\u308b\u6fc3\u5ea6\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u5404\u6fc3\u5ea6\u306b\u3064\u3044\u3066\u3001\u65e5\u5185\u304a\u3088\u30733\u65e5\u9593\u306e\u6e2c\u5b9a\uff08N=9\uff09\u306e\u5e73\u5747\u5024\u3092\u7528\u3044\u3066\u8a55\u4fa1\u3057\u307e\u3057\u305f\u3002\u30e1\u30bd\u30c3\u30c9\u306e\u7cbe\u5ea6\u306f\u3001\u65e2\u77e5\u6fc3\u5ea6\u306b\u5bfe\u3059\u308b\u5b9f\u6e2c\u5b9a\u91cf\u6fc3\u5ea6\u306e\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\uff08%\uff09\u3068\u3057\u3066\u5b9a\u7fa9\u3055\u308c\u3001\u65e5\u9593\u518d\u73fe\u6027\u306f\u76f8\u5bfe\u6a19\u6e96\u504f\u5dee\uff08%RSD\uff09\u306b\u3088\u3063\u3066\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u3088\u308a\u3001\u672c\u30e1\u30bd\u30c3\u30c9\u304c\u3001\u691c\u8a0e\u3057\u305f\u6fc3\u5ea6\u7bc4\u56f2\u306b\u304a\u3044\u3066\u3001\u9ad8\u3044\u7cbe\u5ea6\u3068\u518d\u73fe\u6027\u3092\u6709\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\uff08Table I<\/strong>\u304a\u3088\u3073Table II<\/strong>\u53c2\u7167\uff09\u3002<\/p>\n\n\n\n

Table I:<\/strong> \u5c3f\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30e1\u30bd\u30c3\u30c9\u306e\u65e5\u9593\u518d\u73fe\u6027\u306b\u95a2\u3059\u308b\u5b9f\u9a13\u7d50\u679c\uff08%RSD\uff09<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u6210\u5206<\/strong><\/th>LLOQ (5 ng\/mL)<\/strong><\/th>Low QC (25 ng\/mL)<\/strong><\/th>Med QC (250 ng\/mL)<\/strong><\/th>High QC (750 ng\/mL)<\/strong><\/th><\/tr><\/thead>
\u03948-THC-COOH<\/td>2.14%<\/td>3.81%<\/td>1.87%<\/td>1.76%<\/td><\/tr>
\u03949-THC-COOH<\/td>1.04%<\/td>2.13%<\/td>1.56%<\/td>0.79%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Table II:<\/strong> \u5c3f\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30e1\u30bd\u30c3\u30c9\u306e\u7cbe\u5ea6\u306b\u95a2\u3059\u308b\u5b9f\u9a13\u7d50\u679c\uff08%R\uff09<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u6210\u5206<\/strong><\/th>LLOQ (5 ng\/mL)<\/strong><\/th>Low QC (25 ng\/mL)<\/strong><\/th>Med QC (250 ng\/mL)<\/strong><\/th>High QC (750 ng\/mL)<\/strong><\/th><\/tr><\/thead>
\u03948-THC-COOH<\/td>83.0%<\/td>94.7%<\/td>93.6%<\/td>96.3%<\/td><\/tr>
\u03949-THC-COOH<\/td>80.6%<\/td>98.1%<\/td>90.5%<\/td>95.2%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

2. \u8840\u6db2<\/strong><\/p>\n\n\n\n

\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6027\u80fd<\/h3>\n\n\n\n

Raptor FluoroPhenyl 2.7 \u00b5m, 100 x 3.0 mm\u30ab\u30e9\u30e0\uff08cat.# 9319A1E\uff09\u3092\u7528\u3044\u305f13\u5206\u9593\u306e\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u5206\u96e2\uff08\u7dcf\u5206\u6790\u6642\u959316\u5206\uff09\u306b\u3088\u308a\u30013\u7d44\u306e\u7570\u6027\u4f53\u3001\u8a086\u7a2e\u985e\u306e\u5206\u6790\u5bfe\u8c61\u6210\u5206\u3092\u826f\u597d\u306b\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u76f4\u7dda\u6027\u3000<\/h3>\n\n\n\n

1\/x \u91cd\u307f\u4ed8\u3051\u7dda\u5f62\u56de\u5e30\u3092\u7528\u3044\u3066\u76f4\u7dda\u6027\u3092\u691c\u8a3c\u3057\u305f\u3068\u3053\u308d\u3001\u3059\u3079\u3066\u306e\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u304a\u3044\u3066\u30010.99\u4ee5\u4e0a\u306e\u8a31\u5bb9\u53ef\u80fd\u306ar2<\/sup>\u5024\u3092\u793a\u3057\u3001\u512a\u308c\u305f\u76f4\u7dda\u6027\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u300211-OH-\u03948-THC\u300111-OH-\u03949-THC\u3001\u03948-THC\u3001\u03949-TH\u306e\u6821\u6b63\u6fc3\u5ea6\u7bc4\u56f2\u306f0.5\uff5e100 ng\/mL\u306b\u3001\u03948-THC-COOH\u304a\u3088\u3073\u03949-THC-COOH\u306f2.5\uff5e500 ng\/mL\uff08\u8840\u6db2\u691c\u4f53\u4e2d\u306b\u304a\u3051\u308b\u5178\u578b\u7684\u306a\u6fc3\u5ea6\u5206\u5e03\u3092\u30ab\u30d0\u30fc\uff09\u306b\u305d\u308c\u305e\u308c\u8a2d\u5b9a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u7531\u6765\u306e\u5e72\u6e09\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u30d6\u30e9\u30f3\u30af\u8840\u6db2\u30b5\u30f3\u30d7\u30eb3\u691c\u4f53\u3092\u524d\u51e6\u7406\u3057\u3066\u5206\u6790\u3057\u3001\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u7531\u6765\u3057\u306a\u3044\u30b7\u30b0\u30ca\u30eb\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u304c\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u7531\u6765\u306e\u5e72\u6e09\u306f\u8a8d\u3081\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u307e\u305f\u3001\u3082\u3063\u3068\u3082\u9ad8\u3044\u6821\u6b63\u6fc3\u5ea6\uff081000 ng\/mL\u307e\u305f\u306f500 ng\/mL\uff09\u30b5\u30f3\u30d7\u30eb\u306e\u6ce8\u5165\u5f8c\u306b\u30e1\u30bf\u30ce\u30fc\u30eb\u30d6\u30e9\u30f3\u30af\u3092\u6ce8\u5165\u3057\u3001\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3057\u305f\u3068\u3053\u308d\u3001\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306f\u89b3\u5bdf\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

\u518d\u73fe\u6027\u3068\u7cbe\u5ea6<\/h3>\n\n\n\n

\u518d\u73fe\u6027\u3068\u7cbe\u5ea6\u306e\u8a55\u4fa1\u306f\u3001LLOQ\uff08\u5b9a\u91cf\u4e0b\u9650\uff09\u3001Low QC\uff08\u4f4e\u6fc3\u5ea6QC\uff09\u3001Medium QC\uff08\u4e2d\u6fc3\u5ea6QC\uff09\u3001High QC\uff08\u9ad8\u6fc3\u5ea6QC\uff09\u306e\u30014\u3064\u306e\u7570\u306a\u308b\u6fc3\u5ea6\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u5404\u6fc3\u5ea6\u306b\u3064\u3044\u3066\u3001\u65e5\u5185\u304a\u3088\u30733\u65e5\u9593\u306e\u6e2c\u5b9a\uff08N=9\uff09\u306e\u5e73\u5747\u5024\u3092\u7528\u3044\u3066\u8a55\u4fa1\u3057\u307e\u3057\u305f\u3002\u65e5\u9593\u518d\u73fe\u6027\u306f\u76f8\u5bfe\u6a19\u6e96\u504f\u5dee\uff08%RSD\uff09\u3068\u3057\u307e\u3057\u305f\u3002Table III<\/strong>\u304a\u3088\u3073Table IV<\/strong>\u306e\u7d50\u679c\u304b\u3089\u3001\u672c\u30e1\u30bd\u30c3\u30c9\u304c\u3001\u691c\u8a0e\u3057\u305f\u6fc3\u5ea6\u7bc4\u56f2\u306b\u304a\u3044\u3066\u3001\u9ad8\u3044\u7cbe\u5ea6\u3068\u518d\u73fe\u6027\u3092\u6709\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

Table III:<\/strong> \u5168\u8840\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30e1\u30bd\u30c3\u30c9\u306e\u65e5\u9593\u6a19\u6e96\u504f\u5dee\u306b\u95a2\u3059\u308b\u5b9f\u9a13\u7d50\u679c\uff08%RSD\uff09<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u6210\u5206<\/strong><\/th>LLOQ (0.5\/2.5 ng\/mL)<\/strong><\/th>Low QC (5\/25 ng\/mL)<\/strong><\/th>Med QC (45\/225 ng\/mL)<\/strong><\/th>High QC (75\/375 ng\/mL)<\/strong><\/th><\/tr><\/thead>
11-OH-\u03948-THC<\/td>6.73%<\/td>6.12%<\/td>3.65%<\/td>3.44%<\/td><\/tr>
11-OH-\u03949-THC<\/td>9.90%<\/td>4.13%<\/td>3.61%<\/td>3.37%<\/td><\/tr>
\u03948-THC-COOH*<\/td>4.63%<\/td>4.15%<\/td>2.20%<\/td>3.33%<\/td><\/tr>
\u03949-THC-COOH*<\/td>4.04%<\/td>2.13%<\/td>1.78%<\/td>2.74%<\/td><\/tr>
\u03948-THC<\/td>11.4%<\/td>7.77%<\/td>8.89%<\/td>6.95%<\/td><\/tr>
\u03949-THC<\/td>9.32%<\/td>4.00%<\/td>1.56%<\/td>2.23%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*\u6ce8\uff1a\u0394-8\/9-THC-COOH\u306e\u30bf\u30fc\u30b2\u30c3\u30c8QC\u6fc3\u5ea6\u306f5\u500d\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

Table IV:<\/strong> \u5168\u8840\u4e2d\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30e1\u30bd\u30c3\u30c9\u306e\u7cbe\u5ea6\u306b\u95a2\u3059\u308b\u5b9f\u9a13\u7d50\u679c\uff08%\uff09<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u6210\u5206<\/strong><\/th>LLOQ (0.5\/2.5 ng\/mL)<\/strong><\/th>Low QC (5\/25 ng\/mL)<\/strong><\/th>Med QC (45\/225 ng\/mL)<\/strong><\/th>High QC (75\/375 ng\/mL)<\/strong><\/th><\/tr><\/thead>
11-OH-\u03948-THC<\/td>86.2%<\/td>92.6%<\/td>97.3%<\/td>96.7%<\/td><\/tr>
11-OH-\u03949-THC<\/td>91.1%<\/td>94.8%<\/td>97.2%<\/td>97.6%<\/td><\/tr>
\u03948-THC-COOH*<\/td>96.8%<\/td>96.0%<\/td>97.1%<\/td>97.2%<\/td><\/tr>
\u03949-THC-COOH*<\/td>93.6%<\/td>95.9%<\/td>98.4%<\/td>97.9%<\/td><\/tr>
\u03948-THC<\/td>90.9%<\/td>93.4%<\/td>92.4%<\/td>94.4%<\/td><\/tr>
\u03949-THC<\/td>91.1%<\/td>93.1%<\/td>98.8%<\/td>98.2%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*\u6ce8\uff1a\u0394-8\/9-THC-COOH\u306e\u30bf\u30fc\u30b2\u30c3\u30c8QC\u6fc3\u5ea6\u306f5\u500d\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u6210\u5206\u9593\u306e\u76f8\u4e92\u5e72\u6e09<\/h3>\n\n\n\n

\u540c\u91cd\u4f53\u307e\u305f\u306f\u69cb\u9020\u7684\u306b\u985e\u4f3c\u3057\u305f12\u7a2e\u985e\u306e\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u306b\u3088\u308b\u5e72\u6e09\u306b\u3064\u3044\u3066\u3082\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u5177\u4f53\u7684\u306b\u306f\u3001\u30ab\u30f3\u30ca\u30d3\u30b8\u30d0\u30ea\u30f3\uff08CBDV\uff09\uff1b\u30ab\u30f3\u30ca\u30d3\u30b8\u30aa\u30fc\u30eb\uff08CBD\uff09\uff1b\u30ab\u30f3\u30ca\u30d3\u30b2\u30ed\u30fc\u30eb\uff08CBG\uff09\uff1b\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30d0\u30ea\u30f3\uff08THCV\uff09\uff1b\u30a8\u30ad\u30bd\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\uff08\u30a8\u30ad\u30bd-THC\uff09\uff1b9\uff08S\uff09-\u30d8\u30ad\u30b5\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\uff089\uff08S\uff09-HHC\uff09\uff1b\u30ab\u30f3\u30ca\u30d3\u30b7\u30af\u30ed\u30fc\u30eb\uff08CBL\uff09\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\uff1b 9(R)-\u30d8\u30ad\u30b5\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb(9(R)-HHC)\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\uff08CBN\uff09\uff1b9(S)-\u03946a,10a-THC\uff08\u039410-THC\uff09\uff1b\u30ab\u30f3\u30ca\u30d3\u30af\u30ed\u30e1\u30f3\uff08CBC\uff09\uff1b\u304a\u3088\u3073\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\u9178A\uff08THCA-A\uff09\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3057\u3001\u958b\u767a\u3057\u305f\u5206\u6790\u65b9\u6cd5\u3067\u8a55\u4fa1\u3057\u305f\u3068\u3053\u308d\u3001\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3055\u308c\u305f\u5168\u3066\u306e\u5316\u5408\u7269\u306f\u5206\u6790\u5bfe\u8c61\u6210\u5206\u304b\u3089\u826f\u597d\u306b\u5206\u96e2\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002\u7279\u306b\u3001CBL\u306f\u03949-THC\u3068\u540c\u3058MRM\u30a6\u30a3\u30f3\u30c9\u30a6\u3067\u6eb6\u51fa\u3057\u307e\u3059\u304c\u3001\u4fdd\u6301\u6642\u9593\u306b\u660e\u78ba\u306a\u5dee\u304c\u3042\u308b\u305f\u3081\uff0811.16\u5206\u306811.40\u5206\uff09\u3001\u8aa4\u540c\u5b9a\u306e\u30ea\u30b9\u30af\u306f\u6975\u3081\u3066\u4f4e\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089\u3001\u672c\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u3053\u308c\u3089\u985e\u4f3c\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u306b\u3088\u308b\u5206\u6790\u5bfe\u8c61\u6210\u5206\u3078\u306e\u5e72\u6e09\u306f\u8a8d\u3081\u3089\u308c\u305a\u3001\u9ad8\u3044\u9078\u629e\u6027\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\uff08Figure 5<\/strong>\u53c2\u7167\uff09\u3002<\/p>\n\n\n

Figure 5:<\/strong> 12\u7a2e\u985e\u306e\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u304b\u3089\u306e\u03948\/9-THC\u7570\u6027\u4f53\u3068\u4ee3\u8b1d\u7269\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0<\/div>
\n
\"\u03949-THC;<\/div>

LC_CF0827<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Precursor<\/th>Product 1<\/th>Product 2<\/th><\/tr><\/thead>\n
1.<\/td>CBDV<\/a><\/td>3.22<\/td>286.9<\/td>165.1<\/td>122.9<\/td><\/tr>\n
2.<\/td>11-OH-\u03948-THC<\/a><\/td>4.60<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
3.<\/td>11-OH-\u03948-THC<\/a><\/td>4.94<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
4.<\/td>\u03948-THC-COOH<\/a><\/td>4.98<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
5.<\/td>\u03948-THC-COOH<\/a><\/td>5.77<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
6.<\/td>CBD<\/a><\/td>6.20<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
7.<\/td>THCV<\/a><\/td>6.85<\/td>286.9<\/td>165.1<\/td>122.9<\/td><\/tr>\n
8.<\/td>CBG<\/a><\/td>6.87<\/td>317.0<\/td>193.1<\/td>123.0<\/td><\/tr>\n
9.<\/td>exo-THC<\/a><\/td>9.88<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Precursor<\/th>Product 1<\/th>Product 2<\/th><\/tr><\/thead>
10.<\/td>\u03948-THC<\/a><\/td>10.80<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
11.<\/td>9(S)-HHC<\/a><\/td>11.05<\/td>317.0<\/td>193.0<\/td>123.1<\/td><\/tr>\n
12.<\/td>\u03949-THC<\/a><\/td>11.16<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
13.<\/td>CBL<\/a><\/td>11.40<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
14.<\/td>9(R)-HHC<\/a><\/td>11.85<\/td>317.0<\/td>193.0<\/td>123.1<\/td><\/tr>\n
15.<\/td>CBN<\/a><\/td>12.82<\/td>311.1<\/td>223.0<\/td>293.2<\/td><\/tr>\n
16.<\/td>\u039410-THC<\/a><\/td>13.05<\/td>315.0<\/td>193.0<\/td>259.2<\/td><\/tr>\n
17.<\/td>CBC<\/a><\/td>13.96<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
18.<\/td>THCA-A<\/a><\/td>14.08<\/td>359.3<\/td>341.2<\/td>219.0<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306e\u5229\u70b9<\/h2>\n\n\n\n

\u4e0d\u6d3b\u6027\u51e6\u7406\u3055\u308c\u305fHPLC\u30ab\u30e9\u30e0\u306f\u5f93\u6765\u306e\u30b9\u30c6\u30f3\u30ec\u30b9\u30ab\u30e9\u30e0\u306b\u6bd4\u3079\u3066\u591a\u304f\u306e\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u3002Restek\u306e\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306b\u306f\u3001\u30ab\u30e9\u30e0\u5185\u90e8\u306e\u30b9\u30c6\u30f3\u30ec\u30b9\u92fc\u8868\u9762\u306b\u9ad8\u5ea6\u306a\u4e0d\u6d3b\u6027\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u304c\u65bd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u3063\u3066\u3001\u91d1\u5c5e\u8868\u9762\u5168\u4f53\u306b\u5e83\u304f\u5206\u6790\u6210\u5206\u304c\u5438\u7740\u305b\u305a\u3001\u30ad\u30ec\u30fc\u30c8\u5316\u304c\u5927\u5e45\u306b\u6291\u5236\u3055\u308c\u307e\u3059\u3002\u9178\u6027\u5316\u5408\u7269\u306f\u30ad\u30ec\u30fc\u30c8\u5316\u3057\u3084\u3059\u3044\u305f\u3081\u3001\u30d4\u30fc\u30af\u5f62\u72b6\u60aa\u5316\u3001\u691c\u51fa\u611f\u5ea6\u4f4e\u4e0b\u306e\u50be\u5411\u304c\u3042\u308a\u307e\u3059\u304c\u3001Restek\u306e\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306b\u3088\u3063\u3066\u3053\u308c\u3089\u306e\u6539\u5584\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u3002\u5b9f\u969b\u306e\u6bd4\u8f03\u8a66\u9a13\u3067\u306f\u3001Raptor FluoroPhenyl\u30ab\u30e9\u30e0\uff08\u5f93\u6765\u578b\uff09\u3068Raptor\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u3092\u7528\u3044\u3066\u5206\u6790\u3092\u884c\u3063\u305f\u3068\u3053\u308d\uff08Figure 6<\/strong>\u53c2\u7167\uff09\u3001\u89aa\u5316\u5408\u7269\u306b\u3064\u3044\u3066\u306f\u4e21\u30ab\u30e9\u30e0\u9593\u3067\u5927\u304d\u306a\u9055\u3044\u306f\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u5316\u5408\u7269\u3084\u30ab\u30eb\u30dc\u30ad\u30b7\u5316\u5408\u7269\u3067\u306f\u3001\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u30d4\u30fc\u30af\u9762\u7a4d\u3068\u30d4\u30fc\u30af\u9ad8\u3055\u304c\u660e\u3089\u304b\u306b\u5897\u52a0\u3057\u3001\u691c\u51fa\u611f\u5ea6\u304c\u5411\u4e0a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u30a4\u30ca\u30fc\u30c8HPLC\u30ab\u30e9\u30e0\u306f\u3001\u5f93\u6765\u306e\u30b9\u30c6\u30f3\u30ec\u30b9\u30ab\u30e9\u30e0\u3067\u306f\u56f0\u96e3\u3060\u3063\u305f\u5206\u6790\u6210\u5206\uff08\u30d2\u30c9\u30ed\u30ad\u30b7\u304a\u3088\u3073\u30ab\u30eb\u30dc\u30ad\u30b7\uff09\u306e\u5206\u6790\u3092\u53ef\u80fd\u306b\u3057\u3001\u30e9\u30dc\u306e\u5206\u6790\u7cbe\u5ea6\u3068\u52b9\u7387\u3092\u5927\u304d\u304f\u5411\u4e0a\u3055\u305b\u307e\u3059\u3002<\/p>\n\n\n

Figure 6:<\/strong> \u03949-THC\u3001\u03948-THC\u304a\u3088\u3073\u4ee3\u8b1d\u7269\u306b\u95a2\u3059\u308bRaptor FluoroPhenyl\u30ab\u30e9\u30e0\u3068Raptor\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306e\u6bd4\u8f03\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0<\/div>
\n
\"Comparison<\/div>

LC_CF0829<\/p>

<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u7d50\u8ad6\u3000<\/h2>\n\n\n\n

\u30d2\u30c8\u5c3f\u4e2d\u304a\u3088\u3073\u5168\u8840\u4e2d\u306eTHC\u7570\u6027\u4f53\u3092\u4fe1\u983c\u6027\u9ad8\u304f\u6b63\u3057\u304f\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306e2\u3064\u306e\u9769\u65b0\u7684\u306a\u5206\u6790\u65b9\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u5404\u30e1\u30bd\u30c3\u30c9\u306b\u304a\u3044\u3066\u3001\u5168\u8840\u304a\u3088\u3073\u5c3f\u306e\u4e21\u65b9\u306e\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u306b\u5bfe\u3057\u3066\u3001\u7c21\u4fbf\u306a\u6db2\u6db2\u62bd\u51fa\u6cd5\u3092\u9069\u7528\u3057\u307e\u3057\u305f[3,4]\u3002\u5c3f\u5206\u6790\u306b\u304a\u3044\u3066\u306f\u3001THC\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u4ee3\u8b1d\u7269\u3092\u7570\u6027\u4f53\u9593\u3067\u5b8c\u5168\u306b\u5206\u96e2\u53ef\u80fd\u306aLC-MS\/MS\u30e1\u30bd\u30c3\u30c9\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u03948-THC\u3068\u03949-THC\u306a\u3069\u3001\u985e\u4f3c\u69cb\u9020\u3092\u6301\u3064\u7570\u6027\u4f53\u306e\u5b9a\u6027\u3092\u660e\u78ba\u306b\u884c\u3044\u3001\u6b63\u78ba\u306a\u5b9a\u91cf\u5831\u544a\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u6b21\u306b\u3001\u5168\u8840\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u3067\u306f\u3001\u4e8b\u4ef6\u767a\u751f\u6642\u306e\u88ab\u9a13\u8005\u306e\u4e2d\u6bd2\u72b6\u614b\u8a55\u4fa1\u3092\u76ee\u7684\u3068\u3057\u3066\u3001\u89aa\u5316\u5408\u7269\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u4ee3\u8b1d\u7269\u3001\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u4ee3\u8b1d\u7269\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3059\u308b\u65b9\u6cd5\u3092\u69cb\u7bc9\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u624b\u6cd5\u306f\u3001\u9ad8\u901f\u3067\u5805\u7262\u304b\u3064\u9ad8\u611f\u5ea6\u3067\u3042\u308b\u3053\u3068\u3082\u78ba\u8a8d\u3055\u308c\u3066\u304a\u308a\u3001\u4e8b\u4ef6\u767a\u751f\u6642\u306e\u6b63\u78ba\u306a\u8a3a\u65ad\u3084\u03948-THC\u3068\u03949-THC\u306e\u6442\u53d6\u8b58\u5225\u306b\u5f93\u4e8b\u3057\u3066\u3044\u308b\u81e8\u5e8a\u691c\u67fb\u30e9\u30dc\u3084\u6bd2\u7269\u691c\u67fb\u30e9\u30dc\u306b\u3068\u3063\u3066\u3001\u6975\u3081\u3066\u6709\u7528\u306a\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3068\u306a\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n
    \n
  1. E.L. Karschner, M.J. Swortwood-Gates, M.A. Huestis, Identifying and Quantifying Cannabinoids in Biological Matrices in the Medical and Legal Cannabis Era. Clinical Chemistry 66 (7) (2020) 888-914. https:\/\/academic.oup.com\/clinchem\/article\/66\/7\/888\/5867829?login=false<\/a><\/li>\n\n\n\n
  2. Mayo Clinic Laboratories, Marijuana: Comprehensive urine drug testing to confirm and monitor use  https:\/\/www.mayocliniclabs.com\/test-catalog\/drug-book\/specific-drug-groups\/marijuana<\/a> (Accessed April 1, 2025).<\/li>\n\n\n\n
  3. Virginia Department of Forensic Science, Toxicology Procedures Manual, 2024, https:\/\/dfs.virginia.gov\/wp-content\/uploads\/220-D100%20Toxicology%20Procedures%20Manual-2816-67ec03f17b822.pdf\u00a0<\/a><\/li>\n\n\n\n
  4. N.B. Tiscione, R . Miller, X. Shan, J. Sprague, D.T. Yeatman. An efficient, robust method for the determination of cannabinoids in whole blood by LC-MS-MS. J Anal. Toxicol., 40 (2016) (8) 639-648. https:\/\/doi.org\/10.1093\/jat\/bkw063<\/a><\/li>\n<\/ol>\n\n\n\n

    \u3053\u306e\u30e1\u30bd\u30c3\u30c9\u306f\u7814\u7a76\u7528\u9014\u306e\u307f\u306b\u958b\u767a\u3055\u308c\u3066\u304a\u308a\u3001\u8a3a\u65ad\u306b\u4f7f\u7528\u3059\u308b\u306b\u306f\u3055\u3089\u306a\u308b\u691c\u8a3c\u304c\u5fc5\u8981\u3067\u3059\u3002<\/em><\/p>\n\n\n\n

    <\/div>\n\n\n
    \n

    Products Mentioned<\/h3>\n
    \n
    \n
    \n
    \n Catalog No. 9319A1E <\/a> <\/div>\n
    \n
    Raptor FluoroPhenyl, 2.7 \u00b5m, 100 x 3.0 mm HPLC\u30ab\u30e9\u30e0<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 9319A1E-T <\/a> <\/div>\n
    \n
    Raptor Inert FluoroPhenyl, 2.7 \u00b5m, 100 x 3.0 mm HPLC\u30ab\u30e9\u30e0<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34090 <\/a> <\/div>\n
    \n
    delta 8-Tetrahydrocannabinol (Delta 8-THC) Standard, 1000 \u00b5g\/mL in P&T Methanol, 1 mL\/ampul<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34067 <\/a> <\/div>\n
    \n
    delta 9-Tetrahydrocannabinol (Delta 9-THC) Standard, 1000 \u00b5g\/mL, P&T Methanol, 1 mL\/ampul<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34068 <\/a> <\/div>\n
    \n
    (\u00b1)11-nor-9-carboxy-Delta-9-THC Standard, 100 \u00b5g\/mL, methanol, 1 mL\/ampul<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
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